Name: Microlunatus ginsengisoli DSM 17942
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 12626

Type strain:

Species: Microlunatus ginsengisoli

Culture col. no.: DSM 17942, CIP 109594, KCTC 13940, Gsoil 633, JCM 16929, JCM 15306

Strain history: CIP <- 2007, DSMZ <- S. T. Lee <- W. T. Im, Korea Ad. Inst. Technol., Guseong-dong, Daejon, Korea: strain Gsoil 633

NCBI tax ID(s): 363863 (species)

SI-ID 302767

DSM 17942, KCTC 13940, JCM 15306, CIP 109594

Section

Microlunatus ginsengisoli DSM 17942 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from soil, ginseng field.

coccus-shaped
Gram-positive
aerobe
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Propionibacteriales*
	Family	*Propionibacteriaceae*
	Genus	*Microlunatus*
	Species	**Microlunatus ginsengisoli**
	Full Scientific Name (LPSN)	Microlunatus ginsengisoli Cui et al. 2007

Information on morphological and physiological properties Morphology

[Ref.: #31819]	Gram stain	positive
[Ref.: #67771]	Gram stain	positive
[Ref.: #119627]	Gram stain	positive

[Ref.: #31819]	Cell width	0.5-0.8 µm

[Ref.: #31819]	Cell shape	coccus-shaped
[Ref.: #119627]	Cell shape	coccus-shaped

[Ref.: #31819]	Motility	no
[Ref.: #119627]	Motility	no

[Ref.: #19757]	Colony color	Honey yellow (1005)
[Ref.: #19757]	Incubation period	10-14 days
[Ref.: #19757]	Cultivation medium used	ISP 3

[Ref.: #31819]

Pigment production

yes

[Ref.: #19757]	Multicellular complex forming ability	no
[Ref.: #19757]	Medium Name (multicellularity)	ISP 3

Information on culture and growth conditions Culture and growth conditions

[Ref.: #7279]

Culture medium

MICROLUNATUS MEDIUM (DSMZ Medium 776)

[Ref.: #7279]

Culture medium growth

[Ref.: #7279]

Culture medium link

Medium recipe at MediaDive

[Ref.: #7279]

Culture medium composition

expand / minimize

[Ref.: #19757]

Culture medium

ISP 3

[Ref.: #19757]

Culture medium growth

[Ref.: #19757]

Culture medium composition

expand / minimize

[Ref.: #33312]

Culture medium

MEDIUM 135 - for Microlunatus phosphovorus and Microsphaera multipartita

[Ref.: #33312]

Culture medium growth

[Ref.: #33312]

Culture medium composition

expand / minimize

[Ref.: #119627]

Culture medium

CIP Medium 135

[Ref.: #119627]

Culture medium growth

[Ref.: #119627]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #7279]	growth	28 °C
[Ref.: #19757]	optimum	28 °C
[Ref.: #31819]	growth	16-37 °C
[Ref.: #31819]	optimum	20-30 °C
[Ref.: #33312]	growth	25 °C
[Ref.: #67770]	growth	28 °C
[Ref.: #67771]	growth	30 °C

[Ref.: #7279]	Temperature range	mesophilic
[Ref.: #19757]	Temperature range	mesophilic
[Ref.: #33312]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #67771]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #31819]			optimum	7
[Ref.: #31819]			growth	5.5-8.5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #31819]	Oxygen tolerance	aerobe
[Ref.: #67771]	Oxygen tolerance	aerobe

[Ref.: #31819]

Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #31819]		NaCl		growth	04-05	%
[Ref.: #31819]		NaCl		optimum	4.5	%

[Ref.: #67770]

Observation

quinones: MK-9(H₄)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #31819]	alanine ChEBI	+	carbon source
[Ref.: #31819]	asparagine ChEBI	+	carbon source
[Ref.: #31819]	aspartate ChEBI	+	carbon source
[Ref.: #68379]	D-glucose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-mannitol ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-ribose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-xylose ChEBI	-	fermentation	* from API Coryne
[Ref.: #31819]	dextrin ChEBI	+	carbon source
[Ref.: #68379]	esculin ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #31819]	fructose ChEBI	+	carbon source
[Ref.: #68379]	gelatin ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #68379]	glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #31819]	histidine ChEBI	+	carbon source
[Ref.: #31819]	lactate ChEBI	+	carbon source
[Ref.: #68379]	lactose ChEBI	-	fermentation	* from API Coryne
[Ref.: #31819]	malate ChEBI	+	carbon source
[Ref.: #68379]	maltose ChEBI	-	fermentation	* from API Coryne
[Ref.: #31819]	nitrate ChEBI	+	reduction
[Ref.: #119627]	nitrate ChEBI	+	reduction
[Ref.: #68379]	nitrate ChEBI	-	reduction	* from API Coryne
[Ref.: #119627]	nitrite ChEBI	-	reduction
[Ref.: #31819]	phenylalanine ChEBI	+	carbon source
[Ref.: #31819]	proline ChEBI	+	carbon source
[Ref.: #31819]	propionate ChEBI	+	carbon source
[Ref.: #31819]	raffinose ChEBI	+	carbon source
[Ref.: #31819]	rhamnose ChEBI	+	carbon source
[Ref.: #31819]	ribitol ChEBI	+	carbon source
[Ref.: #31819]	ribose ChEBI	+	carbon source
[Ref.: #31819]	salicin ChEBI	+	carbon source
[Ref.: #31819]	sorbitol ChEBI	+	carbon source
[Ref.: #31819]	sucrose ChEBI	+	carbon source
[Ref.: #68379]	sucrose ChEBI	+	fermentation	* from API Coryne
[Ref.: #31819]	trehalose ChEBI	+	carbon source
[Ref.: #68379]	urea ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #31819]	valerate ChEBI	+	carbon source
[Ref.: #31819]	xylose ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #119627]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68379]	alkaline phosphatase	+	3.1.3.1	* from API Coryne
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68379]	alpha-glucosidase	+	3.2.1.20	* from API Coryne, API zym
[Ref.: #68379]	beta-galactosidase	+	3.2.1.23	* from API Coryne, API zym
[Ref.: #68379]	beta-glucosidase	+	3.2.1.21	* from API Coryne, API zym
[Ref.: #68379]	beta-glucuronidase	+	3.2.1.31	* from API Coryne
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #31819]	catalase	+	1.11.1.6
[Ref.: #119627]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68379]	gelatinase	+		* from API Coryne
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68379]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API Coryne
[Ref.: #119627]	oxidase	-
[Ref.: #68379]	pyrazinamidase	-	3.5.1.B15	* from API Coryne
[Ref.: #68379]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API Coryne
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #119627]	urease	+	3.5.1.5
[Ref.: #68379]	urease	+	3.5.1.5	* from API Coryne
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #19757]	299	-	-	-	+	+	+	+	+	+	+	+	not determinedn.d.	-	-	-	-	-	-	+	-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #19757]	581	not determinedn.d.	+	-	+	-	+	-	-	-	-	+	-	+	+	-	+	+	+	+	+
[Ref.: #119627]	11985	-	-	+	-	-	+	-	-	-	-	+	+	-	+	-	+	+	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #7279]	Sample type/isolated from	soil, ginseng field
[Ref.: #7279]	Geographic location (country and/or sea, region)	Pocheon Province
[Ref.: #7279]	Country	Republic of Korea
[Ref.: #7279]	Country ISO 3 Code	KOR
[Ref.: #7279]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Soil from a ginseng field
[Ref.: #67770]	Geographic location (country and/or sea, region)	Pocheon Province
[Ref.: #67770]	Country	Republic of Korea
[Ref.: #67770]	Country ISO 3 Code	KOR
[Ref.: #67770]	Continent	Asia

[Ref.: #67771]	Sample type/isolated from	From soil of the ginseng field
[Ref.: #67771]	Country	Republic of Korea
[Ref.: #67771]	Country ISO 3 Code	KOR
[Ref.: #67771]	Continent	Asia

[Ref.: #119627]	Sample type/isolated from	Environment, Soil, ginseng field
[Ref.: #119627]	Geographic location (country and/or sea, region)	Pocheon
[Ref.: #119627]	Country	Republic of Korea
[Ref.: #119627]	Country ISO 3 Code	KOR
[Ref.: #119627]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Engineered	#Agriculture	#Field
#Environmental	#Terrestrial	#Soil
#Host	#Plants	#Herbaceous plants (Grass,Crops)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AB245389 (>99% sequence identity) for Microlunatus ginsengisoli subclade from Microbeatlas

Aquatic Samples	28
Soil Samples	427
Animal Samples	94
Plant Samples	9
Total Samples	558

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #7279]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #19757]	Biosafety level	1	Risk group (German classification)
[Ref.: #119627]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #7279]	Microlunatus ginsengisoli gene for 16S rRNA, partial sequence, strain: Gsoil 633	AB245389	1472	ENA	363863 tax ID

[Ref.: #7279]	GC-content	69.8 mol%
[Ref.: #67770]	GC-content	69.8 mol%	high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #7279]

Culture collection no.

DSM 17942, CIP 109594, KCTC 13940, Gsoil 633, JCM 16929, JCM 15306

[Ref.: #81840]

SI-ID 302767

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Microlunatus elymi sp. nov., a novel actinobacterium isolated from rhizospheric soil of the wild plant Elymus tsukushiensis.	Son JS, Lee SY, Hwang YJ, Shin JH, Ghim SY	Int J Syst Evol Microbiol	10.1099/ijsem.0.004428	2020	*
Phylogeny	Microlunatus parietis sp. nov., isolated from an indoor wall.	Kampfer P, Schafer J, Lodders N, Martin K	Int J Syst Evol Microbiol	10.1099/ijs.0.020115-0	2009	*
Phylogeny	Microlunatus ginsengisoli sp. nov., isolated from soil of a ginseng field.	Cui YS, Im WT, Yin CR, Yang DC, Lee ST	Int J Syst Evol Microbiol	10.1099/ijs.0.64395-0	2007	*

References

#7279

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 17942

#19757

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#31819

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #28086 (see below)

#33312 ; Curators of the CIP;
#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#68379

Automatically annotated from API Coryne .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#81840

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID302767.1 )

#119627 Collection of Institut Pasteur ; Curators of the CIP; CIP 109594

#28086

IJSEM 713 2007 ( DOI 10.1099/ijs.0.64395-0 , PubMed 17392192 )

* These data were automatically processed and therefore are not curated

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