Microlunatus phosphovorus (DSM 10555, JCM 9379, NM-1)

Name: Microlunatus phosphovorus (DSM 10555, JCM 9379, NM-1)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 12625

Type strain

Strain Designation NM-1

Culture col. no. DSM 10555 JCM 9379 NM-1 ATCC 700054 CIP 104466 7 more

NCBI tax ID(s) 29405

Special Collections DSMZ JCM KCTC CIP Wink compendium

History <- JCM <- K. Nakamura, NM-1 K. Nakamura NM-1. CIP <- 1995, K. Nakamura, Nat. Inst. of Bioscience Human Technol., Ibaraki, Japan: strain NM-1

Links

Microlunatus phosphovorus NM-1 is an obligate aerobe, Gram-positive, coccus-shaped bacterium that was isolated from activated sludge.

Gram-positive coccus-shaped obligate aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 10852

LMG 18116,ATCC 700054,CIP 104466,DSM 10555,JCM 9379,NCIMB 13414,VKM Ac-1990,KCTC 9798,HAMBI 2303,NBRC 101784

@ref 20215

Last LPSN update 2026-02-09 11:20:53

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Propionibacteriales

Family Propionibacteriaceae

Genus Microlunatus

Species Microlunatus phosphovorus

Full scientific name Microlunatus phosphovorus Nakamura et al. 1995

BacDive ID	Other strains from **Microlunatus phosphovorus** (3)	Type strain
149945	M. phosphovorus CCUG 38730, CCM 4645
163117	M. phosphovorus JCM 21246, IAM 14536
166028	M. phosphovorus JCM 9380, FERM P-10115, KCTC 9799, LMG 18117

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Confidence
121810	positive	coccus-shaped
125438			94.5
125439	positive		96.9

Colony morphology

@ref	Colony color	Incubation period	Medium used
18357	Ivory (1014)	10-14 days	5006
18357	Ivory (1014)	10-14 days	5425
18357	Ivory (1014)	10-14 days	5428
18357	Ivory (1014)	10-14 days	DSM 776
18357	Transparent	10-14 days	ISP 2

Multicellular morphology

@ref	Forms multicellular complex	Medium name
18357		ISP 2
18357		5006
18357		5425
18357		5428
18357		DSM 776

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
3973	MICROLUNATUS MEDIUM (DSMZ Medium 776)	Medium recipe at MediaDive	Name: MICROLUNATUS MEDIUM (DSMZ Medium 776) Composition: KH2PO4 0.5 g/l Na glutamate 0.5 g/l Yeast extract 0.5 g/l Peptone 0.5 g/l Glucose 0.5 g/l (NH4)2SO4 0.1 g/l MgSO4 x 7 H2O 0.1 g/l Distilled water
18357	ISP 2		Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
18357	5006		Medium: 5006 Name: Composition (g/l) Sucrose 3,0 Dextrin 15,0 Meat extract 1,0 Yeast extract 2,0 Tryptone soy broth (Oxoid) 5,0 NaCl 0,5 K2HPO4 0,5 MgSO4 x 7 H2O 0,5 FeSO4 x 7 H2O 0,01 Agar 20,0 Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7,3 Usage: Maintenance Organisms: All Actinomycetes
18357	5425		Name: Brain Heart Infusion (BHI) Agar; 5425 Composition: Calf brain infusion 200.0 g/l Beef heart infusion 250.0 g/l Casein (meat) peptone 10.0 g/l Glucose 2.0 g/l NaCl 5.0 g/l Na2HPO4 2.5 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.4 Usage: Maintenance and revitalization Organisms: Dermatophilus congolensis, Nocardia brasieliensis, N. asteroides, Rhodococcus equi, R. fascians, R. rhodochrous, Streptomyces thermoviol aceus subsp. apingens, Coryneform species
18357	5428
18357	DSM 776		Name: DSM 776; MICROLUNATUS MEDIUM Glucose 0.5 g Peptone 0.5 g Yeast extract 0.5 g Na glutamate 0.5 g KH2PO4 0.5 g (NH4)2SO4 0.1 g MgSO4 x 7 H2O 0.1 g Distilled water 1000.0 ml Adjust pH to 7.0.
37178	MEDIUM 135 - for Microlunatus phosphovorus and Microsphaera multipartita		Distilled water make up to (1000.000 ml);Magnesium sulphate heptahydrate (0.100 g);Agar (18.000 g);Glucose (0.500 g);Yeast extract (0.500 g);Ammonium sulphate (0.100 g);Peptone (0.500 g);Potassium di-hydrogen phosphate (0.440 g);Sodium glutamate (0.500 g)
121810	CIP Medium 135	Medium recipe at CIP
121810	CIP Medium 216	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
3973	positive	growth	30
18357	positive	optimum	28
37178	positive	growth	30
67770	positive	growth	25
121810	negative	growth	41
121810	negative	growth	37
121810	negative	growth	22
121810	negative	growth	10
121810	negative	growth	5
121810	positive	growth	30
121810	negative	growth	45

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
121810	obligate aerobe
125439	obligate aerobe	98.8

Murein

@ref	Murein short key	Type
3973	A42.01	A3gamma' LL-Dpm-Gly

Observation

67770

Observationquinones: MK-9(H₄)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68371	27613 ChEBI	amygdalin	-	builds acid from	from API 50CH acid
68371	18305 ChEBI	arbutin	-	builds acid from	from API 50CH acid
68368	29016 ChEBI	arginine	+	hydrolysis	from API 20E
68371	17057 ChEBI	cellobiose	-	builds acid from	from API 50CH acid
68368	16947 ChEBI	citrate	+	assimilation	from API 20E
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
68371	15824 ChEBI	D-fructose	-	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	12936 ChEBI	D-galactose	-	builds acid from	from API 50CH acid
68379	17634 ChEBI	D-glucose	-	fermentation	from API Coryne
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68379	16899 ChEBI	D-mannitol	-	fermentation	from API Coryne
68371	16899 ChEBI	D-mannitol	-	builds acid from	from API 50CH acid
68371	16024 ChEBI	D-mannose	-	builds acid from	from API 50CH acid
68379	16988 ChEBI	D-ribose	-	fermentation	from API Coryne
68371	16988 ChEBI	D-ribose	-	builds acid from	from API 50CH acid
68371	17924 ChEBI	D-sorbitol	-	builds acid from	from API 50CH acid
68371	16443 ChEBI	D-tagatose	-	builds acid from	from API 50CH acid
68379	65327 ChEBI	D-xylose	-	fermentation	from API Coryne
68371	65327 ChEBI	D-xylose	-	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
68379	4853 ChEBI	esculin	+	hydrolysis	from API Coryne
121810	4853 ChEBI	esculin	+	hydrolysis
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
68379	5291 ChEBI	gelatin	-	hydrolysis	from API Coryne
68368	5291 ChEBI	gelatin	-	hydrolysis	from API 20E
68371	28066 ChEBI	gentiobiose	-	builds acid from	from API 50CH acid
68371	24265 ChEBI	gluconate	-	builds acid from	from API 50CH acid
68371	17754 ChEBI	glycerol	-	builds acid from	from API 50CH acid
68379	28087 ChEBI	glycogen	-	fermentation	from API Coryne
68371	28087 ChEBI	glycogen	-	builds acid from	from API 50CH acid
121810	606565 ChEBI	hippurate	-	hydrolysis
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
68371	30849 ChEBI	L-arabinose	-	builds acid from	from API 50CH acid
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	-	builds acid from	from API 50CH acid
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
68379	17716 ChEBI	lactose	-	fermentation	from API Coryne
68371	17716 ChEBI	lactose	-	builds acid from	from API 50CH acid
68368	25094 ChEBI	lysine	-	degradation	from API 20E
68379	17306 ChEBI	maltose	-	fermentation	from API Coryne
68371	17306 ChEBI	maltose	-	builds acid from	from API 50CH acid
68371	6731 ChEBI	melezitose	-	builds acid from	from API 50CH acid
68371	28053 ChEBI	melibiose	-	builds acid from	from API 50CH acid
68371	320061 ChEBI	methyl alpha-D-glucopyranoside	-	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
68371	59640 ChEBI	N-acetylglucosamine	-	builds acid from	from API 50CH acid
68379	17632 ChEBI	nitrate	-	reduction	from API Coryne
121810	17632 ChEBI	nitrate	+	reduction
121810	16301 ChEBI	nitrite	-	reduction
68368	18257 ChEBI	ornithine	-	degradation	from API 20E
68371		Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371		Potassium 5-ketogluconate	-	builds acid from	from API 50CH acid
68371	16634 ChEBI	raffinose	-	builds acid from	from API 50CH acid
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
68371	17814 ChEBI	salicin	-	builds acid from	from API 50CH acid
68371	28017 ChEBI	starch	-	builds acid from	from API 50CH acid
68379	17992 ChEBI	sucrose	+	fermentation	from API Coryne
68371	17992 ChEBI	sucrose	-	builds acid from	from API 50CH acid
68371	27082 ChEBI	trehalose	-	builds acid from	from API 50CH acid
68368	27897 ChEBI	tryptophan	-	energy source	from API 20E
68371	32528 ChEBI	turanose	-	builds acid from	from API 50CH acid
68379	16199 ChEBI	urea	+	hydrolysis	from API Coryne
68368	16199 ChEBI	urea	+	hydrolysis	from API 20E
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

Metabolite production

@ref	Chebi-ID	Metabolite
68368	15688 ChEBI	acetoin	from API 20E
68368	16136 ChEBI	hydrogen sulfide	from API 20E
121810	35581 ChEBI	indole
68368	35581 ChEBI	indole	from API 20E

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Indole test
68368	15688 ChEBI	acetoin	-		from API 20E
68368	35581 ChEBI	indole		-	from API 20E

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
121810	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68379	alkaline phosphatase	+	3.1.3.1	from API Coryne
68382	alpha-galactosidase	+	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68379	alpha-glucosidase	+	3.2.1.20	from API Coryne
68382	alpha-mannosidase	+	3.2.1.24	from API zym
68368	arginine dihydrolase	+	3.5.3.6	from API 20E
68382	beta-galactosidase	+	3.2.1.23	from API zym
121810	beta-galactosidase	+	3.2.1.23
68379	beta-galactosidase	+	3.2.1.23	from API Coryne
68368	beta-galactosidase	+	3.2.1.23	from API 20E
68382	beta-glucosidase	+	3.2.1.21	from API zym
68379	beta-glucosidase	+	3.2.1.21	from API Coryne
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68379	beta-glucuronidase	-	3.2.1.31	from API Coryne
121810	catalase	+	1.11.1.6
121810	coagulase	-
121810	DNase	-
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
121810	gamma-glutamyltransferase	-	2.3.2.2
121810	gelatinase	-
68379	gelatinase	-		from API Coryne
68368	gelatinase	-		from API 20E
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
121810	lysine decarboxylase	-	4.1.1.18
68368	lysine decarboxylase	-	4.1.1.18	from API 20E
68382	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API zym
68379	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API Coryne
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
121810	ornithine decarboxylase	-	4.1.1.17
68368	ornithine decarboxylase	-	4.1.1.17	from API 20E
121810	oxidase	+
68379	pyrazinamidase	-	3.5.1.B15	from API Coryne
68379	pyrrolidonyl arylamidase	-	3.4.19.3	from API Coryne
68382	trypsin	+	3.4.21.4	from API zym
68368	tryptophan deaminase	-	4.1.99.1	from API 20E
121810	urease	+	3.5.1.5
68379	urease	+	3.5.1.5	from API Coryne
68368	urease	+	3.5.1.5	from API 20E
68382	valine arylamidase	+		from API zym

API 20E

@ref	ONPG	ADH (Arg)	LDC (Lys)	ODC	CIT	H2S productionH2S	URE	TDA (Trp)	IND	Acetoin production (Voges Proskauer test)VP	GEL	GLU	MAN	INO	Sor	RHA	SAC	MEL	AMY	ARA	OX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	OF-O	OF-F
18357	+	+	-	-	+	-	+	-	-	-	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API coryne

@ref	Reduction of nitrateNIT	PYZ	PYRA	PAL	beta GUR	beta GAL	alpha GLU	beta NAG	ESC	URE	GEL	Control fermentationControl	GLU	RIB	XYL	MAN	MAL	LAC	SAC	GLYG	CAT
18357	-	-	-	+	-	+	+	+	+	+	-	not determinedn.d.	-	-	-	-	-	-	+	-	not determinedn.d.

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
18357	not determinedn.d.	+	+	+	-	+	+	+	+	+	+	+	+	+	-	+	+	+	+	+
121810	-	+	+	+	-	+	+	-	+	-	+	+	+	+	-	+	+	+	+	-

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
121810	not determinedn.d.	-	-	-	-	-	-	-	-	-	-	+/-	-	-	-	-	-	-	-	-	-	-	-	-	-	+/-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

API biotype100

@ref	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
121810	not determinedn.d.	+	+	+	+	+	-	+	+	+	+	+	+	+	-	+	+	+	+	+	+	-	+	+	+	-	+	+	+	+	+	-	+	+	+	+	+	+	+	-	+	-	+	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	+	+	-	-	-	+	-	-	-	-	-	-	-	+	-	-	+	-	-	-	-	-	-	+	-	-	-	+	-	-	+	+	+	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Waste	#Activated sludge

Isolation

@ref	Sample type	Country	Country ISO 3 Code	Continent	Isolation date
3973	activated sludge	Japan	JPN	Asia
67770	Activated sludge grown on diluted corn steep liquor	Japan	JPN	Asia
121810	Environment, Activated sludge	Japan	JPN	Asia	1989

Taxonmaps

69479

Global distribution of 16S sequence Z78207 (>99% sequence identity) for Microlunatus phosphovorus from Microbeatlas

Aquatic Samples	1937
Soil Samples	494
Animal Samples	341
Plant Samples	69
Total Samples	2841

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
18357	1	German classification According to TRBA 466
3973	1	Risk group (German classification) According to TRBA 466
121810	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM27024v1 assembly for Microlunatus phosphovorus NM-1	complete	GCA_000270245	1032480.4	650716058	1032480	96.25

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	M.phosphovorus 16S rRNA gene	Z78207	1468		29405
3973	Microlunatus phosphovorus gene for 16S rRNA	D26169	1444		1032480

GC content

@ref	GC-content (mol%)	Method
3973	68	high performance liquid chromatography (HPLC)
67770	67.9	high performance liquid chromatography (HPLC)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Microlunatus phosphovorus NM-1
PATRIC: 1032480.4

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	98.80	no
125439	gram_stain	BacteriaNetⓘ	positive	96.90	no
125439	motility	BacteriaNetⓘ	no	75.00	no
125439	spore_formation	BacteriaNetⓘ	no	51.20	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Microlunatus phosphovorus NM-1 NM-1
NCBI: GCA_000270245.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	84.81	no
125438	anaerobic	anaerobicⓘ	no	93.98	yes
125438	aerobic	aerobicⓘ	yes	85.41	no
125438	spore-forming	spore-formingⓘ	no	59.79	no
125438	thermophilic	thermophileⓘ	no	95.00	no
125438	flagellated	motile2+ⓘ	no	94.50	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Dynamics of polyphosphate-accumulating bacteria in wastewater treatment plant microbial communities detected via DAPI (4',6'-diamidino-2-phenylindole) and tetracycline labeling.	Gunther S, Trutnau M, Kleinsteuber S, Hause G, Bley T, Roske I, Harms H, Muller S.	Appl Environ Microbiol	10.1128/aem.01540-08	2009
Metabolism	Glucose metabolism and kinetics of phosphorus removal by the fermentative bacterium Microlunatus phosphovorus.	Santos MM, Lemos PC, Reis MA, Santos H.	Appl Environ Microbiol	10.1128/aem.65.9.3920-3928.1999	1999
Phylogeny	Distribution and phylogenetic analysis of family 19 chitinases in Actinobacteria.	Kawase T, Saito A, Sato T, Kanai R, Fujii T, Nikaidou N, Miyashita K, Watanabe T.	Appl Environ Microbiol	10.1128/aem.70.2.1135-1144.2004	2004
Pathogenicity	Classroom microbiome, functional pathways and sick-building syndrome (SBS) in urban and rural schools - Potential roles of indoor microbial amino acids and vitamin metabolites.	Fu X, Ou Z, Zhang M, Meng Y, Li Y, Chen Q, Jiang J, Zhang X, Norback D, Zhao Z, Sun Y.	Sci Total Environ	10.1016/j.scitotenv.2021.148879	2021
Metabolism	Polyphosphate metabolic gene expression analyses reveal mechanisms of phosphorus accumulation and release in Microlunatus phosphovorus strain JN459.	Zhong C, Fu J, Jiang T, Zhang C, Cao G.	FEMS Microbiol Lett	10.1093/femsle/fny034	2018
Genetics	Integrated genomics provides insights into the evolution of the polyphosphate accumulation trait of Ca. Accumulibacter.	Xie X, Deng X, Chen L, Yuan J, Chen H, Wei C, Liu X, Wuertz S, Qiu G.	Environ Sci Ecotechnol	10.1016/j.ese.2023.100353	2024
	[Research progress on the genus Microlunatus].	Zhang C, Yu L, Zhang Y.	Wei Sheng Wu Xue Bao		2017
Genetics	Composition, antibiotic resistance, and virulence analysis of microbiota in dormitory drain pipes.	Hu Y, Zhang K, Li N, Wang S.	Front Microbiol	10.3389/fmicb.2023.1272605	2023
Metabolism	Microbial selection on enhanced biological phosphorus removal systems fed exclusively with glucose.	Begum SA, Batista JR.	World J Microbiol Biotechnol	10.1007/s11274-012-1024-3	2012
Phylogeny	Amplicon-guided isolation and cultivation of previously uncultured microbial species from activated sludge.	Verhoeven MD, Nielsen PH, Dueholm MKD.	Appl Environ Microbiol	10.1128/aem.01151-23	2023
	Population dynamics of phage-host system of Microlunatus phosphovorus indigenous in activated sludge.	Lee SH, Otawa K, Onuki M, Satoh H, Mino T.	J Microbiol Biotechnol		2007
	Composition and biodiversity of soil and root-associated microbiome in Vitis vinifera cultivar Lambrusco distinguish the microbial terroir of the Lambrusco DOC protected designation of origin area on a local scale.	Nanetti E, Palladino G, Scicchitano D, Trapella G, Cinti N, Fabbrini M, Cozzi A, Accetta G, Tassini C, Iannaccone L, Candela M, Rampelli S.	Front Microbiol	10.3389/fmicb.2023.1108036	2023
Enzymology	All1371 is a polyphosphate-dependent glucokinase in Anabaena sp. PCC 7120.	Klemke F, Beyer G, Sawade L, Saitov A, Korte T, Maldener I, Lockau W, Nurnberg DJ, Volkmer T.	Microbiology (Reading)	10.1099/mic.0.081836-0	2014
Phylogeny	Blind spots of universal primers and specific FISH probes for functional microbe and community characterization in EBPR systems.	Yuan J, Deng X, Xie X, Chen L, Wei C, Feng C, Qiu G.	ISME Commun	10.1093/ismeco/ycae011	2024
Metabolism	Accumulation of polyhydroxyalkanoates by Microlunatus phosphovorus under various growth conditions.	Akar A, Akkaya EU, Yesiladali SK, Celikyilmaz G, Cokgor EU, Tamerler C, Tamerler C, Orhon D, Cakar ZP.	J Ind Microbiol Biotechnol	10.1007/s10295-004-0201-2	2006
Metabolism	Effects of influent COD/N ratios on nitrous oxide emission in a sequencing biofilm batch reactor for simultaneous nitrogen and phosphorus removal.	Ge G, Zhao J, Li X, Ding X, Chen A, Chen Y, Hu B, Wang S.	Sci Rep	10.1038/s41598-017-06943-0	2017
Metabolism	Functional Genetic Diversity and Plant Growth Promoting Potential of Polyphosphate Accumulating Bacteria in Soil.	Srivastava S, Anand V, Kaur J, Ranjan M, Bist V, Asif MH, Srivastava S.	Microbiol Spectr	10.1128/spectrum.00345-21	2022
	ATP- and Polyphosphate-Dependent Glucokinases from Aerobic Methanotrophs.	Reshetnikov AS, Solntseva NP, Rozova ON, Mustakhimov II, Trotsenko YA, Khmelenina VN.	Microorganisms	10.3390/microorganisms7020052	2019
Metabolism	Isolation and characterization of a Gram-positive polyphosphate-accumulating bacterium.	Onda S, Takii S.	J Gen Appl Microbiol	10.2323/jgam.48.125	2002
	The composition of environmental microbiota in three tree fruit packing facilities changed over seasons and contained taxa indicative of L. monocytogenes contamination.	Rolon ML, Tan X, Chung T, Gonzalez-Escalona N, Chen Y, Macarisin D, LaBorde LF, Kovac J.	Microbiome	10.1186/s40168-023-01544-8	2023
Phylogeny	SyntTax: a web server linking synteny to prokaryotic taxonomy.	Oberto J.	BMC Bioinformatics	10.1186/1471-2105-14-4	2013
Metabolism	Strictly polyphosphate-dependent glucokinase in a polyphosphate-accumulating bacterium, Microlunatus phosphovorus.	Tanaka S, Lee SO, Hamaoka K, Kato J, Takiguchi N, Nakamura K, Ohtake H, Kuroda A.	J Bacteriol	10.1128/jb.185.18.5654-5656.2003	2003
Metabolism	Microbial selection of polyphosphate-accumulating bacteria in activated sludge wastewater treatment processes for enhanced biological phosphate removal.	Mino T.	Biochemistry (Mosc)		2000
Genetics	Prokaryotic diversity of tropical coastal sand dunes ecosystem using metagenomics.	Shet SA, Garg S.	3 Biotech	10.1007/s13205-021-02809-5	2021
	Polyamine profiles within genera of the class Actinobacteria with LL-diaminopimelic acid in the peptidoglycan.	Busse HJ, Schumann P.	Int J Syst Bacteriol	10.1099/00207713-49-1-179	1999
	Mass-immigration determines the assembly of activated sludge microbial communities.	Dottorini G, Michaelsen TY, Kucheryavskiy S, Andersen KS, Kristensen JM, Peces M, Wagner DS, Nierychlo M, Nielsen PH.	Proc Natl Acad Sci U S A	10.1073/pnas.2021589118	2021
Enzymology	Coevolution of both Thermostability and Activity of Polyphosphate Glucokinase from Thermobifida fusca YX.	Zhou W, Huang R, Zhu Z, Zhang YPJ.	Appl Environ Microbiol	10.1128/aem.01224-18	2018
	Characterisation of Phosphate Accumulating Organisms and Techniques for Polyphosphate Detection: A Review.	Tarayre C, Nguyen HT, Brognaux A, Delepierre A, De Clercq L, Charlier R, Michels E, Meers E, Delvigne F.	Sensors (Basel)	10.3390/s16060797	2016
Phylogeny	Evolutionary and Comparative Analysis of Bacterial Nonhomologous End Joining Repair.	Sharda M, Badrinarayanan A, Seshasayee ASN.	Genome Biol Evol	10.1093/gbe/evaa223	2020
Metabolism	The genome sequence of Propionibacterium acidipropionici provides insights into its biotechnological and industrial potential.	Parizzi LP, Grassi MC, Llerena LA, Carazzolle MF, Queiroz VL, Lunardi I, Zeidler AF, Teixeira PJ, Mieczkowski P, Rincones J, Pereira GA.	BMC Genomics	10.1186/1471-2164-13-562	2012
Phylogeny	A Critical Assessment of the Microorganisms Proposed to be Important to Enhanced Biological Phosphorus Removal in Full-Scale Wastewater Treatment Systems.	Stokholm-Bjerregaard M, McIlroy SJ, Nierychlo M, Karst SM, Albertsen M, Nielsen PH.	Front Microbiol	10.3389/fmicb.2017.00718	2017
Metabolism	Intracellular Accumulation of Glycine in Polyphosphate-Accumulating Organisms in Activated Sludge, a Novel Storage Mechanism under Dynamic Anaerobic-Aerobic Conditions.	Nguyen HT, Kristiansen R, Vestergaard M, Wimmer R, Nielsen PH.	Appl Environ Microbiol	10.1128/aem.01012-15	2015
Metabolism	Metabolic model for the filamentous 'Candidatus Microthrix parvicella' based on genomic and metagenomic analyses.	McIlroy SJ, Kristiansen R, Albertsen M, Karst SM, Rossetti S, Nielsen JL, Tandoi V, Seviour RJ, Nielsen PH.	ISME J	10.1038/ismej.2013.6	2013
Phylogeny	'Candidatus Competibacter'-lineage genomes retrieved from metagenomes reveal functional metabolic diversity.	McIlroy SJ, Albertsen M, Andresen EK, Saunders AM, Kristiansen R, Stokholm-Bjerregaard M, Nielsen KL, Nielsen PH.	ISME J	10.1038/ismej.2013.162	2014
Genetics	Synthetic spike-in standards for high-throughput 16S rRNA gene amplicon sequencing.	Tourlousse DM, Yoshiike S, Ohashi A, Matsukura S, Noda N, Sekiguchi Y.	Nucleic Acids Res	10.1093/nar/gkw984	2017
Enzymology	Structural basis for phosphatidylinositol-phosphate biosynthesis.	Clarke OB, Tomasek D, Jorge CD, Dufrisne MB, Kim M, Banerjee S, Rajashankar KR, Shapiro L, Hendrickson WA, Santos H, Mancia F.	Nat Commun	10.1038/ncomms9505	2015
Phylogeny	Comparative phylogenetic assignment of environmental sequences of genes encoding 16S rRNA and numerically abundant culturable bacteria from an anoxic rice paddy soil.	Hengstmann U, Chin KJ, Janssen PH, Liesack W.	Appl Environ Microbiol	10.1128/aem.65.11.5050-5058.1999	1999
Genetics	Genome-Based Taxonomic Classification of the Phylum Actinobacteria.	Nouioui I, Carro L, Garcia-Lopez M, Meier-Kolthoff JP, Woyke T, Kyrpides NC, Pukall R, Klenk HP, Goodfellow M, Goker M.	Front Microbiol	10.3389/fmicb.2018.02007	2018
Metabolism	Carbon uptake bioenergetics of PAOs and GAOs in full-scale enhanced biological phosphorus removal systems.	Chen L, Chen H, Hu Z, Tian Y, Wang C, Xie P, Deng X, Zhang Y, Tang X, Lin X, Li B, Wei C, Qiu G	Water Res	10.1016/j.watres.2022.118258	2022
Metabolism	The PolS-PolR Two-Component System Regulates Genes Involved in Poly-P Metabolism and Phosphate Transport in Microlunatus phosphovorus.	Zhong C, Zhang P, Liu C, Liu M, Chen W, Fu J, Qi X, Cao G	Front Microbiol	10.3389/fmicb.2019.02127	2019
Metabolism	Metagenomic characterization of 'Candidatus Defluviicoccus tetraformis strain TFO71', a tetrad-forming organism, predominant in an anaerobic-aerobic membrane bioreactor with deteriorated biological phosphorus removal.	Nobu MK, Tamaki H, Kubota K, Liu WT	Environ Microbiol	10.1111/1462-2920.12383	2014
Genetics	Deciphering the genome of polyphosphate accumulating actinobacterium Microlunatus phosphovorus.	Kawakoshi A, Nakazawa H, Fukada J, Sasagawa M, Katano Y, Nakamura S, Hosoyama A, Sasaki H, Ichikawa N, Hanada S, Kamagata Y, Nakamura K, Yamazaki S, Fujita N	DNA Res	10.1093/dnares/dss020	2012
Phylogeny	Isolation, characterization of bacteriophages specific to Microlunatus phosphovorus and their application for rapid host detection.	Lee SH, Onuki M, Satoh H, Mino T	Lett Appl Microbiol	10.1111/j.1472-765X.2006.01840.x	2006
Phylogeny	Microlunatus panaciterrae sp. nov., a beta-glucosidase-producing bacterium isolated from soil in a ginseng field.	An DS, Im WT, Yoon MH.	Int J Syst Evol Microbiol	10.1099/ijs.0.65004-0	2008
Phylogeny	Microlunatus aurantiacus sp. nov., a novel actinobacterium isolated from a rhizosphere soil sample.	Wang YX, Cai M, Zhi XY, Zhang YQ, Tang SK, Xu LH, Cui XL, Li WJ.	Int J Syst Evol Microbiol	10.1099/ijs.0.65518-0	2008
Phylogeny	Propionicimonas paludicola gen. nov., sp. nov., a novel facultatively anaerobic, Gram-positive, propionate-producing bacterium isolated from plant residue in irrigated rice-field soil.	Akasaka H, Ueki A, Hanada S, Kamagata Y, Ueki K.	Int J Syst Evol Microbiol	10.1099/ijs.0.02764-0	2003
Phylogeny	Micropruina glycogenica gen. nov., sp. nov., a new Gram-positive glycogen-accumulating bacterium isolated from activated sludge.	Shintani T, Liu WT, Hanada S, Kamagata Y, Miyaoka S, Suzuki T, Nakamura K.	Int J Syst Evol Microbiol	10.1099/00207713-50-1-201	2000
Phylogeny	Tessaracoccus bendigoensis gen. nov., sp. nov., a gram-positive coccus occurring in regular packages or tetrads, isolated from activated sludge biomass.	Maszenan AM, Seviour RJ, Patel BK, Schumann P, Rees GN.	Int J Syst Bacteriol	10.1099/00207713-49-2-459	1999
Metabolism	Friedmanniella antarctica gen. nov., sp. nov., an LL-diaminopimelic acid-containing actinomycete from Antarctic sandstone.	Schumann P, Prauser H, Rainey FA, Stackebrandt E, Hirsch P.	Int J Syst Bacteriol	10.1099/00207713-47-2-278	1997
Phylogeny	Microlunatus parietis sp. nov., isolated from an indoor wall.	Kampfer P, Schafer J, Lodders N, Martin K	Int J Syst Evol Microbiol	10.1099/ijs.0.020115-0	2009
Phylogeny	Microlunatus phosphovorus gen. nov., sp. nov., a new gram-positive polyphosphate-accumulating bacterium isolated from activated sludge.	Nakamura K, Hiraishi A, Yoshimi Y, Kawaharasaki M, Masuda K, Kamagata Y	Int J Syst Bacteriol	10.1099/00207713-45-1-17	1995

References

#3973	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 10555
#18357	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#37178	; Curators of the CIP;
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68368	Automatically annotated from API 20E .
#68371	Automatically annotated from API 50CH acid .
#68379	Automatically annotated from API Coryne .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#121810	Collection of Institut Pasteur ; Curators of the CIP; CIP 104466
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Microlunatus phosphovorus (DSM 10555, JCM 9379, NM-1)

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API 20E

API coryne

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