Microlunatus spumicola (DSM 21308, ACM 5121, JCM 16540)

Name: Microlunatus spumicola (DSM 21308, ACM 5121, JCM 16540)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 12615

Type strain

Strain Designation J14, Ben-107

Culture col. no. DSM 21308 ACM 5121 JCM 16540 Ben 107 J14 3 more

NCBI tax ID(s) 81499

Special Collections DSMZ JCM CIP

History DSMZ <- R.J. Seviour; J14 JCM <-- DSM 21308 <-- R. J. Seviour J14 (=Ben 107). CIP <- 2001, M. Dougall, Victoria, Australia: strain Ben-107

Links

Microlunatus spumicola J14 is a Gram-positive, coccus-shaped bacterium that was isolated from activated sludge, sewage treatment plant.

Gram-positive coccus-shaped genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 87673

DSM 21308,ACM 5121,CIP 107258,JCM 16540

@ref 20215

Last LPSN update 2026-05-29 08:27:50

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Propionibacteriales

Family Propionibacteriaceae

Genus Microlunatus

Species Microlunatus spumicola

Full scientific name Microlunatus spumicola corrig. (Maszenan et al. 1999) Nouioui et al. 2018

Synonyms (2)

Friedmanniella spumicola
Microlunatus spumicolus

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Confidence
123276	positive	coccus-shaped
125438			90
125439	positive		97.531

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
15510	R2A MEDIUM (DSMZ Medium 830)	Medium recipe at MediaDive	Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
32863	MEDIUM 4 - Columbia agar with 20 % sterile defibrinated horse serum		Horse serum (200.000 ml);Columbia agar (39.000 g);Distilled water make up to (1000.000 ml)
123276	CIP Medium 4	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
15510	positive	growth	25-28
32863	positive	growth	25
67770	positive	growth	25
123276	positive	growth	10-37
123276	negative	growth	41
123276	negative	growth	45

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
125439	obligate aerobe	99.47

Halophily

@ref	Salt	Tested relation	Concentration
123276	NaCl	growth	0 %
123276	NaCl	growth	2 %
123276	NaCl	growth	4 %
123276	NaCl	growth	6 %
123276	NaCl	growth	8 %
123276	NaCl	growth	10 %

Observation

67770

Observationquinones: MK-9(H₄), MK-7(H₂)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
123276	16947 ChEBI	citrate	-	carbon source
123276	4853 ChEBI	esculin	+	hydrolysis
123276	606565 ChEBI	hippurate	-	hydrolysis
123276	17632 ChEBI	nitrate	-	reduction
123276	16301 ChEBI	nitrite	-	reduction

Metabolite production

@ref	Chebi-ID	Metabolite	Production
123276	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Methylred-test
123276	15688 ChEBI	acetoin	-
123276	17234 ChEBI	glucose		-

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
123276	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	+	3.2.1.51	from API zym
68382	alpha-galactosidase	+	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	-	3.2.1.23	from API zym
123276	beta-galactosidase	+	3.2.1.23
68382	beta-glucosidase	+	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
123276	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
123276	gamma-glutamyltransferase	+	2.3.2.2
123276	gelatinase	-
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
123276	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	-		from API zym
123276	ornithine decarboxylase	-	4.1.1.17
123276	oxidase	-
123276	phenylalanine ammonia-lyase	-	4.3.1.24
68382	trypsin	-	3.4.21.4	from API zym
123276	tryptophan deaminase	-
123276	urease	-	3.5.1.5
68382	valine arylamidase	+		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
123276	-	-	+	+	-	+	+	-	-	-	+	-	+	-	-	+	+	+	-	+

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Waste	#Activated sludge
#Engineered	#Waste	#Water treatment plant

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Isolation date
15510	activated sludge, sewage treatment plant		Australia	AUS	Australia and Oceania
67770	Stable foam on the surface of the aerobic reactor in an activated sludge plant treating mainly wastewater from an orange-juice-processing plant	Mildura, Victoria	Australia	AUS	Australia and Oceania
123276	Environment, Activated sludge		Australia	AUS	Australia and Oceania	1992

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
15510	1	Risk group (German classification) According to TRBA 466
123276	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	IMG accession	NCBI tax ID	Score	BV-BRC accession	INSDC accession
66792	Friedmanniella spumicola DSM 21308	complete	2891974067	81499	99.26
66792	Microlunatus spumicola strain DSM 21308	contig		81499	75.63	81499.3
124043	ASM3953644v1 assembly for Microlunatus spumicola JCM 16540	scaffold		81499	64.19		GCA_039536445

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
15510	Friedmanniella spumicola 16S ribosomal RNA gene, partial sequence	AF062535	1475		81499

GC content

@ref	GC-content (mol%)	Method
15510	69
67770	69	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Microlunatus spumicola strain DSM 21308
BV-BRC: 81499.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	99.47	no
125439	motility	BacteriaNetⓘ	no	73.91	no
125439	gram_stain	BacteriaNetⓘ	positive	97.53	no
125439	spore_formation	BacteriaNetⓘ	no	84.08	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Microlunatus spumicola strain DSM 21308
PATRIC: 81499.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	85.53	no
125438	anaerobic	anaerobicⓘ	no	96.98	no
125438	aerobic	aerobicⓘ	yes	88.10	no
125438	spore-forming	spore-formingⓘ	no	61.38	no
125438	thermophilic	thermophileⓘ	no	97.00	yes
125438	flagellated	motile2+ⓘ	no	90.00	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Friedmanniella spumicola sp. nov. and Friedmanniella capsulata sp. nov. from activated sludge foam: gram-positive cocci that grow in aggregates of repeating groups of cocci.	Maszenan AM, Seviour RJ, Patel BK, Schumann P, Burghardt J, Webb RI, Soddell JA, Rees GN	Int J Syst Bacteriol	10.1099/00207713-49-4-1667	1999

References

#15510	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 21308
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#32863	; Curators of the CIP;
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68382	Automatically annotated from API zym .
#123276	Collection of Institut Pasteur ; Curators of the CIP; CIP 107258
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Microlunatus spumicola (DSM 21308, ACM 5121, JCM 16540)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Halophily

Observation

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_039536445

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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