Name: Microlunatus spumicola J14
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 12615

Type strain:

Species: Microlunatus spumicola

Strain Designation: J14, Ben-107

Culture col. no.: DSM 21308, ACM 5121, JCM 16540, Ben 107, CIP 107258, KACC 14247

Strain history: CIP <- 2001, M. Dougall, Victoria, Australia: strain Ben-107

NCBI tax ID(s): 81499 (species)

SI-ID 87673

ACM 5121, CIP 107258, JCM 16540

Section

Microlunatus spumicola J14 is a spore-forming, mesophilic bacterium that was isolated from activated sludge, sewage treatment plant.

spore-forming
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Propionibacteriales*
	Family	*Propionibacteriaceae*
	Genus	*Microlunatus*
	Species	**Microlunatus spumicola**
	Full Scientific Name (LPSN)	Microlunatus spumicola corrig. (Maszenan et al. 1999) Nouioui et al. 2018

	Synonym	Microlunatus spumicolus
	Synonym	Friedmanniella spumicola

Information on morphological and physiological properties Morphology

[Ref.: #123276]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #123276]	Cell shape	coccus-shaped

[Ref.: #123276]	Motility	no
[Ref.: #69480]	Motility	no Confidence in %96.668

Information on culture and growth conditions Culture and growth conditions

[Ref.: #15510]

Culture medium

R2A MEDIUM (DSMZ Medium 830)

[Ref.: #15510]

Culture medium growth

[Ref.: #15510]

Culture medium link

Medium recipe at MediaDive

[Ref.: #15510]

Culture medium composition

expand / minimize

[Ref.: #32863]

Culture medium

MEDIUM 4 - Columbia agar with 20 % sterile defibrinated horse serum

[Ref.: #32863]

Culture medium growth

[Ref.: #32863]

Culture medium composition

expand / minimize

[Ref.: #123276]

Culture medium

CIP Medium 4

[Ref.: #123276]

Culture medium growth

[Ref.: #123276]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #15510]		growth	25-28 °C
[Ref.: #32863]		growth	25 °C
[Ref.: #67770]		growth	25 °C
[Ref.: #123276]		growth	10-37 °C
[Ref.: #123276]	no	growth	41 °C
[Ref.: #123276]	no	growth	45 °C

[Ref.: #15510]	Temperature range	mesophilic
[Ref.: #32863]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #123276]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #69481]	Ability of spore formation	yes Confidence in %90
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.397

	Salt	Tested relation		Salt conc.
[Ref.: #123276]	NaCl	no	growth	0	%
[Ref.: #123276]	NaCl	no	growth	2	%
[Ref.: #123276]	NaCl	no	growth	4	%
[Ref.: #123276]	NaCl	no	growth	6	%
[Ref.: #123276]	NaCl	no	growth	8	%
[Ref.: #123276]	NaCl	no	growth	10	%

[Ref.: #67770]

Observation

quinones: MK-9(H₄), MK-7(H₂)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #123276]	citrate ChEBI	-	carbon source
[Ref.: #123276]	esculin ChEBI	+	hydrolysis
[Ref.: #123276]	hippurate ChEBI	-	hydrolysis
[Ref.: #123276]	nitrate ChEBI	-	reduction
[Ref.: #123276]	nitrite ChEBI	-	reduction

	Metabolite production	Metabolite	Production
[Ref.: #123276]		indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test	Methylred-test
[Ref.: #123276]		acetoin ChEBI	-
[Ref.: #123276]		glucose ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #123276]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	+	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	+	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #123276]	beta-galactosidase	+	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #123276]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #123276]	gamma-glutamyltransferase	+	2.3.2.2
[Ref.: #123276]	gelatinase	-
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #123276]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #123276]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #123276]	oxidase	-
[Ref.: #123276]	phenylalanine ammonia-lyase	-	4.3.1.24
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #123276]	tryptophan deaminase	-
[Ref.: #123276]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #123276]	10206	-	-	+	+	-	+	+	-	-	-	+	-	+	-	-	+	+	+	-	+

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #15510]	Sample type/isolated from	activated sludge, sewage treatment plant
[Ref.: #15510]	Country	Australia
[Ref.: #15510]	Country ISO 3 Code	AUS
[Ref.: #15510]	Continent	Australia and Oceania

[Ref.: #67770]	Sample type/isolated from	Stable foam on the surface of the aerobic reactor in an activated sludge plant treating mainly wastewater from an orange-juice-processing plant
[Ref.: #67770]	Geographic location (country and/or sea, region)	Mildura, Victoria
[Ref.: #67770]	Country	Australia
[Ref.: #67770]	Country ISO 3 Code	AUS
[Ref.: #67770]	Continent	Australia and Oceania

[Ref.: #123276]	Sample type/isolated from	Environment, Activated sludge
[Ref.: #123276]	Country	Australia
[Ref.: #123276]	Country ISO 3 Code	AUS
[Ref.: #123276]	Continent	Australia and Oceania
[Ref.: #123276]	Isolation date	1992
* marker position based on {}

Isolation sources categories

#Engineered	#Waste	#Activated sludge
#Engineered	#Waste	#Water treatment plant

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #15510]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #123276]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #15510]	Friedmanniella spumicola 16S ribosomal RNA gene, partial sequence	AF062535	1475	ENA	81499 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Microlunatus spumicola strain DSM 21308	81499.3	wgs	PATRIC	81499 tax ID	*
[Ref.: #66792]	Friedmanniella spumicola DSM 21308	2891974067	draft	JGI IMG/M	81499 tax ID	*

[Ref.: #15510]	GC-content	69 mol%
[Ref.: #67770]	GC-content	69 mol%	thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Microlunatus spumicola strain DSM 21308 PATRIC: 81499.3
[Ref.: #69481]	spore-forming	yes	90	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Microlunatus spumicola strain DSM 21308 PATRIC: 81499.3
[Ref.: #69480]	motile	no	91.118	no
[Ref.: #69480]	flagellated	no	98.408	no
[Ref.: #69480]	gram-positive	yes	86.629	no
[Ref.: #69480]	anaerobic	no	99.176	no
[Ref.: #69480]	halophile	no	96.513	no
[Ref.: #69480]	spore-forming	no	85.067	no
[Ref.: #69480]	glucose-util	yes	86.557	no
[Ref.: #69480]	aerobic	yes	93.674	no
[Ref.: #69480]	thermophile	no	99.207	yes
[Ref.: #69480]	glucose-ferment	no	87.172	no

Availability in culture collections External links

[Ref.: #15510]

Culture collection no.

DSM 21308, ACM 5121, JCM 16540, Ben 107, CIP 107258, KACC 14247

[Ref.: #81829]

SI-ID 87673

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Friedmanniella spumicola sp. nov. and Friedmanniella capsulata sp. nov. from activated sludge foam: gram-positive cocci that grow in aggregates of repeating groups of cocci.	Maszenan AM, Seviour RJ, Patel BK, Schumann P, Burghardt J, Webb RI, Soddell JA, Rees GN	Int J Syst Bacteriol	10.1099/00207713-49-4-1667	1999	*

References

#15510

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 21308

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#32863 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68382

Automatically annotated from API zym .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#81829

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID87673.1 )

#123276 Collection of Institut Pasteur ; Curators of the CIP; CIP 107258
* These data were automatically processed and therefore are not curated

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Microlunatus spumicola strain DSM 21308

Microlunatus spumicola strain DSM 21308

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