Name: Isoptericola jiangsuensis CLG
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 12586

Type strain:

Species: Isoptericola jiangsuensis

Strain Designation: CLG

Culture col. no.: DSM 21863, CCTCC AB 208287, JCM 17812

Strain history: DSM 21863 <-- Y. Wu strain CLG.

NCBI tax ID(s): 548579 (species)

SI-ID 400465

DSM 21863

Section

Isoptericola jiangsuensis CLG is a mesophilic, Gram-positive, rod-shaped bacterium that was isolated from beach soil.

Gram-positive
rod-shaped
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Micrococcales*
	Family	*Promicromonosporaceae*
	Genus	*Isoptericola*
	Species	**Isoptericola jiangsuensis**
	Full Scientific Name (LPSN)	Isoptericola jiangsuensis Wu et al. 2010

Information on morphological and physiological properties Morphology

[Ref.: #29424]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #29424]	Cell shape	rod-shaped

[Ref.: #29424]	Motility	no
[Ref.: #69480]	Motility	no Confidence in %94.842

[Ref.: #21363]	Colony color	Sand yellow (1002)
[Ref.: #21363]	Incubation period	10-14 days
[Ref.: #21363]	Cultivation medium used	ISP 2

[Ref.: #21363]	Colony color	Traffic yellow (1023)
[Ref.: #21363]	Incubation period	10-14 days
[Ref.: #21363]	Cultivation medium used	ISP 3

[Ref.: #21363]	Colony color	Honey yellow (1005)
[Ref.: #21363]	Incubation period	10-14 days
[Ref.: #21363]	Cultivation medium used	ISP 4

[Ref.: #21363]	Colony color	Signal yellow (1003)
[Ref.: #21363]	Incubation period	10-14 days
[Ref.: #21363]	Cultivation medium used	ISP 5

[Ref.: #21363]	Colony color	Traffic yellow (1023)
[Ref.: #21363]	Incubation period	10-14 days
[Ref.: #21363]	Cultivation medium used	ISP 7

[Ref.: #21363]	Colony color	Ochre brown (8001)
[Ref.: #21363]	Incubation period	10-14 days
[Ref.: #21363]	Cultivation medium used	suter with tyrosine

[Ref.: #21363]	Colony color	Corn yellow (1006)
[Ref.: #21363]	Incubation period	10-14 days
[Ref.: #21363]	Cultivation medium used	suter without tyrosine

[Ref.: #29424]

Pigment production

yes

[Ref.: #21363]	Multicellular complex forming ability	no
[Ref.: #21363]	Medium Name (multicellularity)	ISP 2

[Ref.: #21363]	Multicellular complex forming ability	no
[Ref.: #21363]	Medium Name (multicellularity)	ISP 3

[Ref.: #21363]	Multicellular complex forming ability	no
[Ref.: #21363]	Medium Name (multicellularity)	ISP 4

[Ref.: #21363]	Multicellular complex forming ability	no
[Ref.: #21363]	Medium Name (multicellularity)	ISP 5

[Ref.: #21363]	Multicellular complex forming ability	no
[Ref.: #21363]	Medium Name (multicellularity)	ISP 7

[Ref.: #21363]	Multicellular complex forming ability	no
[Ref.: #21363]	Medium Name (multicellularity)	Suter with tyrosine

[Ref.: #21363]	Multicellular complex forming ability	no
[Ref.: #21363]	Medium Name (multicellularity)	Suter without tyrosine

Information on culture and growth conditions Culture and growth conditions

[Ref.: #15999]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

[Ref.: #15999]

Culture medium growth

[Ref.: #15999]

Culture medium link

Medium recipe at MediaDive

[Ref.: #15999]

Culture medium composition

expand / minimize

[Ref.: #21363]

Culture medium

ISP 2

[Ref.: #21363]

Culture medium growth

[Ref.: #21363]

Culture medium composition

expand / minimize

[Ref.: #21363]

Culture medium

ISP 3

[Ref.: #21363]

Culture medium growth

[Ref.: #21363]

Culture medium composition

expand / minimize

[Ref.: #21363]

Culture medium

ISP 4

[Ref.: #21363]

Culture medium growth

[Ref.: #21363]

Culture medium composition

expand / minimize

[Ref.: #21363]

Culture medium

ISP 5

[Ref.: #21363]

Culture medium growth

[Ref.: #21363]

Culture medium composition

expand / minimize

[Ref.: #21363]

Culture medium

ISP 7

[Ref.: #21363]

Culture medium growth

[Ref.: #21363]

Culture medium composition

expand / minimize

[Ref.: #21363]

Culture medium

suter with tyrosine

[Ref.: #21363]

Culture medium growth

[Ref.: #21363]

Culture medium composition

expand / minimize

[Ref.: #21363]

Culture medium

suter without tyrosine

[Ref.: #21363]

Culture medium growth

[Ref.: #21363]

Culture medium composition

expand / minimize

[Ref.: #15999]

Culture medium

LB (Luria-Bertani) MEDIUM (DSMZ Medium 381)

[Ref.: #15999]

Culture medium growth

[Ref.: #15999]

Culture medium link

Medium recipe at MediaDive

[Ref.: #15999]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #15999]	growth	28 °C
[Ref.: #29424]	optimum	30 °C
[Ref.: #67770]	growth	28 °C

[Ref.: #15999]	Temperature range	mesophilic
[Ref.: #29424]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #29424]			optimum	7

Information on physiology and metabolism Physiology and metabolism

[Ref.: #29424]	Ability of spore formation	no
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.884

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #21363]		NaCl		maximum	7.5	%
[Ref.: #29424]		NaCl		optimum	2	%

[Ref.: #15999]	Murein short key	A11.31
[Ref.: #15999]	Murein types	A4alpha L-Lys-D-Asp

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #21363]	arabinose ChEBI	+
[Ref.: #21363]	cellulose ChEBI	+
[Ref.: #68379]	D-glucose ChEBI	+	fermentation	* from API Coryne
[Ref.: #68379]	D-mannitol ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-ribose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-xylose ChEBI	+	fermentation	* from API Coryne
[Ref.: #68379]	esculin ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #21363]	fructose ChEBI	+
[Ref.: #68379]	gelatin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #29424]	gluconate ChEBI	+	carbon source
[Ref.: #21363]	glucose ChEBI	+
[Ref.: #68379]	lactose ChEBI	-	fermentation	* from API Coryne
[Ref.: #21363]	mannose ChEBI	+
[Ref.: #21363]	myo-inositol ChEBI	-
[Ref.: #68379]	nitrate ChEBI	+	reduction	* from API Coryne
[Ref.: #21363]	raffinose ChEBI	-
[Ref.: #21363]	rhamnose ChEBI	+
[Ref.: #21363]	sucrose ChEBI	+
[Ref.: #68379]	sucrose ChEBI	+	fermentation	* from API Coryne
[Ref.: #21363]	xylose ChEBI	+
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #68379]	alkaline phosphatase	-	3.1.3.1	* from API Coryne, API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68379]	alpha-glucosidase	-	3.2.1.20	* from API Coryne
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68379]	beta-galactosidase	-	3.2.1.23	* from API Coryne, API zym
[Ref.: #68379]	beta-glucosidase	+	3.2.1.21	* from API Coryne
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68379]	beta-glucuronidase	-	3.2.1.31	* from API Coryne, API zym
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #29424]	gelatinase	+
[Ref.: #68379]	gelatinase	-		* from API Coryne
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API zym
[Ref.: #68379]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API Coryne
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #68379]	pyrazinamidase	+	3.5.1.B15	* from API Coryne
[Ref.: #68379]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API Coryne
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #21363]	492	+	+	-	-	-	-	-	-	+	+/-	-	not determinedn.d.	+	-	+	-	+/-	-	+	+/-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #21363]	1004	not determinedn.d.	-	+/-	+	-	+	+/-	-	-	-	-	-	+/-	-	-	+	-	+	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #15999]	Sample type/isolated from	beach soil
[Ref.: #15999]	Geographic location (country and/or sea, region)	Lian Yungang, Jiangsu province
[Ref.: #15999]	Country	China
[Ref.: #15999]	Country ISO 3 Code	CHN
[Ref.: #15999]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Beach sand
[Ref.: #67770]	Geographic location (country and/or sea, region)	Jiangsu
[Ref.: #67770]	Country	China
[Ref.: #67770]	Country ISO 3 Code	CHN
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental	#Terrestrial	#Coast
#Environmental	#Terrestrial	#Soil

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence EU852101 (>99% sequence identity) for Isoptericola from Microbeatlas

Aquatic Samples	31
Soil Samples	82
Animal Samples	53
Plant Samples	29
Total Samples	195

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #15999]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #15999]	Isoptericola jiangsuensis strain CLG 16S ribosomal RNA gene, partial sequence	EU852101	1478	ENA	548579 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Isoptericola jiangsuensis DSM 21863	GCA_002563715	contig	GenBank	548579 tax ID
[Ref.: #66792]	Isoptericola jiangsuensis strain DSM 21863	548579.3	wgs	PATRIC	548579 tax ID	*
[Ref.: #66792]	Isoptericola jiangsuensis DSM 21863	2630968264	draft	JGI IMG/M	548579 tax ID	*

[Ref.: #15999]	GC-content	70.3 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #29424]	GC-content	70.3 mol%
[Ref.: #67770]	GC-content	73.8 mol%	genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Isoptericola jiangsuensis strain DSM 21863 PATRIC: 548579.3
[Ref.: #69481]	spore-forming	no	82	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Isoptericola jiangsuensis strain DSM 21863 PATRIC: 548579.3
[Ref.: #69480]	gram-positive	yes	89.717	yes
[Ref.: #69480]	anaerobic	no	99.005	no
[Ref.: #69480]	halophile	no	85.464	no
[Ref.: #69480]	spore-forming	no	83.13	yes
[Ref.: #69480]	glucose-util	yes	88.964	yes
[Ref.: #69480]	thermophile	no	97.544	no
[Ref.: #69480]	aerobic	yes	88.859	no
[Ref.: #69480]	motile	no	92.261	no
[Ref.: #69480]	flagellated	no	97.783	yes
[Ref.: #69480]	glucose-ferment	no	73.996	no

Availability in culture collections External links

[Ref.: #15999]

Culture collection no.

DSM 21863, CCTCC AB 208287, JCM 17812

[Ref.: #81800]

SI-ID 400465

Literature:

Topic	Title	Authors	Journal	DOI	Year
Enzymology	Identification of chitinases Is-chiA and Is-chiB from Isoptericola jiangsuensis CLG and their characterization.	Wu Y, Liu F, Liu YC, Zhang ZH, Zhou TT, Liu X, Shen QR, Shen B	Appl Microbiol Biotechnol	10.1007/s00253-010-2917-4	2010	*
Phylogeny	Isoptericola jiangsuensis sp. nov., a chitin-degrading bacterium.	Wu Y, Li WJ, Tian W, Zhang LP, Xu L, Shen QR, Shen B	Int J Syst Evol Microbiol	10.1099/ijs.0.012864-0	2009	*

References

#15999

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 21863

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#21363

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#29424

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #25830 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68379

Automatically annotated from API Coryne .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#81800

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID400465.1 )

#25830

IJSEM 904 2010 ( DOI 10.1099/ijs.0.012864-0 , PubMed 19661504 )

* These data were automatically processed and therefore are not curated

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Isoptericola jiangsuensis strain DSM 21863

Isoptericola jiangsuensis strain DSM 21863

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