Name: Prevotella maculosa W1609
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 12557

Type strain:

Species: Prevotella maculosa

Strain Designation: W1609

Culture col. no.: DSM 19339, CCUG 54766, JCM 15638

Strain history: CCUG 54766 <-- J. Downes <-- W. G. Wade W1609.

NCBI tax ID(s): 439703 (species)

SI-ID 402457

DSM 19339, CCUG 54766

Special Collections

Section

Prevotella maculosa W1609 is an anaerobe, mesophilic, Gram-negative bacterium that was isolated from human oral cavity.

Gram-negative
rod-shaped
anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	09-12-2024 (DD-MM-YYYY)
	Domain	*Bacteria*
	Phylum	*Bacteroidota*
	Class	*Bacteroidia*
	Order	*Bacteroidales*
	Family	*Prevotellaceae*
	Genus	*Prevotella*
	Species	**Prevotella maculosa**
	Full Scientific Name (LPSN)	Prevotella maculosa Downes et al. 2007

Synonym

Segatella maculosa

Information on morphological and physiological properties Morphology

[Ref.: #32300]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %96.337

[Ref.: #32300]	Cell length	2 µm

[Ref.: #32300]	Cell width	0.7 µm

[Ref.: #32300]	Cell shape	rod-shaped

[Ref.: #32300]	Motility	no
[Ref.: #69480]	Motility	no Confidence in %91.5

[Ref.: #60318]

Incubation period

2 days

[Ref.: #32300]

Pigment production

Information on culture and growth conditions Culture and growth conditions

[Ref.: #8056]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #8056]

Culture medium growth

[Ref.: #8056]

Culture medium link

Medium recipe at MediaDive

[Ref.: #8056]

Culture medium composition

expand / minimize

[Ref.: #8056]

Culture medium

PYG MEDIUM (MODIFIED) (DSMZ Medium 104)

[Ref.: #8056]

Culture medium growth

[Ref.: #8056]

Culture medium link

Medium recipe at MediaDive

[Ref.: #8056]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #8056]	growth	37 °C
[Ref.: #60318]	growth	37 °C
[Ref.: #67770]	growth	37 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #8056]	Oxygen tolerance	anaerobe
[Ref.: #32300]	Oxygen tolerance	anaerobe
[Ref.: #60318]	Oxygen tolerance	anaerobe
[Ref.: #69480]	Oxygen tolerance	anaerobe Confidence in %94.937

[Ref.: #69480]	Ability of spore formation	no Confidence in %91.055
[Ref.: #69481]	Ability of spore formation	no Confidence in %100

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #32300]	acetate ChEBI	+	carbon source
[Ref.: #32300]	arabinose ChEBI	+	carbon source
[Ref.: #68380]	arginine ChEBI	-	hydrolysis	* from API rID32A
[Ref.: #32300]	cellobiose ChEBI	+	carbon source
[Ref.: #68367]	cellobiose ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	D-glucose ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	D-mannitol ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	D-mannose ChEBI	-	builds acid from	* from API 20A
[Ref.: #68380]	D-mannose ChEBI	-	fermentation	* from API rID32A
[Ref.: #68367]	D-xylose ChEBI	-	builds acid from	* from API 20A
[Ref.: #32300]	esculin ChEBI	+	hydrolysis
[Ref.: #68367]	esculin ChEBI	-	hydrolysis	* from API 20A
[Ref.: #32300]	fructose ChEBI	+	carbon source
[Ref.: #68367]	gelatin ChEBI	-	hydrolysis	* from API 20A
[Ref.: #32300]	glucose ChEBI	+	carbon source
[Ref.: #68367]	glycerol ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	L-arabinose ChEBI	-	builds acid from	* from API 20A
[Ref.: #68380]	L-glutamate ChEBI	-	degradation	* from API rID32A
[Ref.: #68367]	L-rhamnose ChEBI	-	builds acid from	* from API 20A
[Ref.: #32300]	lactose ChEBI	+	carbon source
[Ref.: #68367]	lactose ChEBI	-	builds acid from	* from API 20A
[Ref.: #32300]	maltose ChEBI	+	carbon source
[Ref.: #68367]	maltose ChEBI	-	builds acid from	* from API 20A
[Ref.: #32300]	mannose ChEBI	+	carbon source
[Ref.: #68367]	melezitose ChEBI	-	builds acid from	* from API 20A
[Ref.: #32300]	melibiose ChEBI	+	carbon source
[Ref.: #68380]	nitrate ChEBI	-	reduction	* from API rID32A
[Ref.: #32300]	raffinose ChEBI	+	carbon source
[Ref.: #68367]	raffinose ChEBI	-	builds acid from	* from API 20A
[Ref.: #68380]	raffinose ChEBI	-	fermentation	* from API rID32A
[Ref.: #32300]	rhamnose ChEBI	+	carbon source
[Ref.: #32300]	salicin ChEBI	+	carbon source
[Ref.: #68367]	salicin ChEBI	+	builds acid from	* from API 20A
[Ref.: #68367]	sorbitol ChEBI	-	builds acid from	* from API 20A
[Ref.: #32300]	succinate ChEBI	+	carbon source
[Ref.: #32300]	sucrose ChEBI	+	carbon source
[Ref.: #68367]	sucrose ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	trehalose ChEBI	-	builds acid from	* from API 20A
[Ref.: #68380]	tryptophan ChEBI	-	energy source	* from API rID32A
[Ref.: #68380]	urea ChEBI	-	hydrolysis	* from API rID32A, API 20A
[Ref.: #32300]	xylose ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #68380]		indole ChEBI	no	* from API rID32A

	Physiological tests	Metabolite	Indole test
[Ref.: #68380]		indole ChEBI	-	* from API rID32A

	Enzyme	Enzyme activity	EC number
[Ref.: #68380]	alanine arylamidase	+	3.4.11.2	* from API rID32A
[Ref.: #32300]	alkaline phosphatase	+	3.1.3.1
[Ref.: #68380]	alkaline phosphatase	+	3.1.3.1	* from API rID32A
[Ref.: #68380]	alpha-arabinosidase	+	3.2.1.55	* from API rID32A
[Ref.: #68380]	alpha-fucosidase	-	3.2.1.51	* from API rID32A
[Ref.: #32300]	alpha-galactosidase	+	3.2.1.22
[Ref.: #68380]	alpha-galactosidase	+	3.2.1.22	* from API rID32A
[Ref.: #68380]	alpha-glucosidase	+	3.2.1.20	* from API rID32A
[Ref.: #68380]	arginine dihydrolase	-	3.5.3.6	* from API rID32A
[Ref.: #68380]	beta-galactosidase	+	3.2.1.23	* from API rID32A
[Ref.: #68380]	beta-glucosidase	+	3.2.1.21	* from API rID32A
[Ref.: #68367]	beta-glucosidase	-	3.2.1.21	* from API 20A
[Ref.: #68380]	beta-glucuronidase	+	3.2.1.31	* from API rID32A
[Ref.: #68367]	gelatinase	-		* from API 20A
[Ref.: #68380]	glutamate decarboxylase	-	4.1.1.15	* from API rID32A
[Ref.: #68380]	glutamyl-glutamate arylamidase	-		* from API rID32A
[Ref.: #68380]	glycin arylamidase	-		* from API rID32A
[Ref.: #68380]	histidine arylamidase	-		* from API rID32A
[Ref.: #68380]	L-arginine arylamidase	-		* from API rID32A
[Ref.: #68380]	leucine arylamidase	-	3.4.11.1	* from API rID32A
[Ref.: #68380]	leucyl glycin arylamidase	+	3.4.11.1	* from API rID32A
[Ref.: #68380]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API rID32A
[Ref.: #68380]	phenylalanine arylamidase	-		* from API rID32A
[Ref.: #68380]	proline-arylamidase	-	3.4.11.5	* from API rID32A
[Ref.: #68380]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API rID32A
[Ref.: #68380]	serine arylamidase	-		* from API rID32A
[Ref.: #68380]	tryptophan deaminase	-	4.1.99.1	* from API rID32A
[Ref.: #68380]	tyrosine arylamidase	-		* from API rID32A
[Ref.: #68380]	urease	-	3.5.1.5	* from API rID32A, API 20A
	Only first 10 entries are displayed. Click here to see all.

API® 20A:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Indole productionIND	Urease/urea hydrolysisURE	Acid from D-glucoseGLU	Acid from D-mannitolMAN	Acid from lactoseLAC	Acid from sucroseSAC	Acid from maltoseMAL	Acid from salicinSAL	Acid from D-xyloseXYL	Acid from L-arabinoseARA	Gelatinase/gelatin hydrolysisGEL	Esculin hydrolysis/beta-glucosidaseESC	Acid from glycerolGLY	Acid from cellobioseCEL	Acid from D-mannoseMNE	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from sorbitolSOR	Acid from L-rhamnoseRHA	Acid from trehaloseTRE	CatalaseCAT	Spores presentSPOR	GramGRAM	Morphology coccus="+" rod="-"COCC
[Ref.: #8056]	2281	not determinedn.d.	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API® rID32A:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Urease/urea hydrolysisURE	Arginine dihydrolaseADH (Arg)	alpha-Galactosidasealpha GAL	beta-Galactosidasebeta GAL	beta-Galactosidase 6-phosphatebeta GP	alpha-Glucosidasealpha GLU	beta-Glucosidasebeta GLU	alpha-Arabinosidasealpha ARA	beta-Glucuronidasebeta GUR	beta-N-Acetyl-beta-glucosaminidasebeta NAG	Fermentation of D-mannoseMNE	Fermentation of raffinoseRAF	Glutamate decarboxylaseGDC	alpha-Fucosidasealpha FUC	Reduction of nitrateNIT	Indole productionIND	Alkaline phosphatasePAL	L-arginine arylamidaseArgA	Proline arylamidaseProA	Leucyl glycin arylamidaseLGA	Phenylalanine arylamidasePheA	Leucine arylamidaseLeuA	Pyrrolidonyl arylamidasePyrA	Tyrosine arylamidaseTyrA	Alanine arylamidaseAlaA	Glycin arylamidaseGlyA	Histidine arylamidaseHisA	Glutamyl-glutamate arylamidaseGGA	Serine arylamidaseSerA
[Ref.: #8056]	13621	-	-	+	+	+/-	+	+	+	+	+	-	-	-	-	-	-	+	-	-	+	-	-	-	-	+	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #8056]	Sample type/isolated from	human oral cavity
[Ref.: #8056]	Geographic location (country and/or sea, region)	Cardiff
[Ref.: #8056]	Country	United Kingdom
[Ref.: #8056]	Country ISO 3 Code	GBR
[Ref.: #8056]	Continent	Europe

[Ref.: #60318]	Sample type/isolated from	Human oral cavity,pericoronitis
[Ref.: #60318]	Sampling date	1989
[Ref.: #60318]	Geographic location (country and/or sea, region)	Cardiff
[Ref.: #60318]	Country	United Kingdom
[Ref.: #60318]	Country ISO 3 Code	GBR
[Ref.: #60318]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Human oral cavity
* marker position based on {}

Isolation sources categories

#Host	#Human	-
#Host Body-Site	#Oral cavity and airways	-
#Host Body-Site	#Oral cavity and airways	#Mouth

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AB547690 (>99% sequence identity) for Prevotella maculosa subclade from Microbeatlas

Aquatic Samples	232
Soil Samples	188
Animal Samples	16676
Plant Samples	57
Total Samples	17153

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #8056]	Pathogenicity (human)	yes, in single cases
[Ref.: #8056]	Biosafety level	1	Risk group (German classification) According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #8056]	Prevotella maculosa strain W1609 16S ribosomal RNA gene, partial sequence	EF534314	1454	GenBank ENA	439703 tax ID
[Ref.: #8056]	Prevotella maculosa strain W1609 16S ribosomal RNA gene, partial sequence	EF534315	1454	GenBank ENA	439703 tax ID
[Ref.: #67770]	Prevotella maculosa gene for 16S ribosomal RNA, partial sequence, strain: JCM 15638	AB547690	1405	GenBank ENA	439703 tax ID

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Prevotella maculosa DSM 19339 = JCM 15638	GCA_000382385	scaffold	GenBank ENA	1122986 tax ID
[Ref.: #67770]	Prevotella maculosa DSM 19339 = JCM 15638	GCA_000613965	contig	GenBank ENA	1122986 tax ID
[Ref.: #66792]	Prevotella maculosa DSM 19339	1122986.3	wgs	PATRIC	1122986 tax ID	*
[Ref.: #66792]	Prevotella maculosa JCM 15638	1236506.4	wgs	PATRIC	1122986 tax ID	*
[Ref.: #66792]	Prevotella maculosa JCM 15638	2585427901	draft	JGI IMG/M	1122986 tax ID	*
[Ref.: #66792]	Prevotella maculosa DSM 19339	2521172638	draft	JGI IMG/M	1122986 tax ID	*

[Ref.: #8056]	GC-content	48 mol%
[Ref.: #67770]	GC-content	48 mol%	high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Prevotella maculosa JCM 15638 PATRIC: 1236506.4
[Ref.: #69481]	spore-forming	spore-formingⓘ	no	100	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Prevotella maculosa DSM 19339 PATRIC: 1122986.3
[Ref.: #69480]	gram-positive	gram-positiveⓘ	no	96.337	yes
[Ref.: #69480]	anaerobic	anaerobicⓘ	yes	94.937	yes
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	91.055	no
[Ref.: #69480]	aerobic	aerobicⓘ	no	93.713	yes
[Ref.: #69480]	thermophilic	thermophileⓘ	no	89.047	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	no	91.5	yes

Availability in culture collections External links

[Ref.: #8056]

Culture collection no.

DSM 19339, CCUG 54766, JCM 15638

[Ref.: #81771]

SI-ID 402457

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Prevotella maculosa sp. nov., isolated from the human oral cavity.	Downes J, Sutcliffe IC, Booth V, Wade WG	Int J Syst Evol Microbiol	10.1099/ijs.0.65281-0	2007	*

References

#8056

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 19339

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#32300

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #28539 (see below)

#60318 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 54766

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68367

Automatically annotated from API 20A .

#68380

Automatically annotated from API rID32A .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#81771

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID402457.1 )

#28539

IJSEM 2936 2007 ( DOI 10.1099/ijs.0.65281-0 , PubMed 18048753 )

* These data were automatically processed and therefore are not curated

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Search for species Segatella maculosa in external resources:
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Prevotella maculosa JCM 15638

Prevotella maculosa DSM 19339

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