Name: Hoylesella timonensis 4401737
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 12556

Type strain:

Species: Hoylesella timonensis

Strain Designation: 4401737

Culture col. no.: JCM 50105, DSM 22865, CCUG 50105, CIP 108522, JCM 15640, KCTC 15080

Strain history: CIP <- 2004, V. Roux, Timone Hosp., Marseille, France: strain 4401737

NCBI tax ID(s): 386414 (species)

SI-ID 302772

CCUG 50105, CIP 108522, JCM 15640, DSM 22865

Special Collections

Section

Hoylesella timonensis 4401737 is an anaerobe, Gram-negative, rod-shaped human pathogen that was isolated from human breast abscess .

Gram-negative
rod-shaped
anaerobe
human pathogen
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacteroidota*
	Class	*Bacteroidia*
	Order	*Bacteroidales*
	Family	*Prevotellaceae*
	Genus	*Hoylesella*
	Species	**Hoylesella timonensis**
	Full Scientific Name (LPSN)	Hoylesella timonensis (Glazunova et al. 2007) Hitch et al. 2023

Synonym

Prevotella timonensis

Information on morphological and physiological properties Morphology

[Ref.: #31893]	Gram stain	negative
[Ref.: #123550]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %94.779

[Ref.: #31893]	Cell length	1.1 µm

[Ref.: #31893]	Cell width	0.4 µm

[Ref.: #31893]	Cell shape	rod-shaped
[Ref.: #123550]	Cell shape	rod-shaped

[Ref.: #31893]	Motility	no
[Ref.: #123550]	Motility	no
[Ref.: #69480]	Motility	no Confidence in %91.5

[Ref.: #58743]

Incubation period

2-3 days

[Ref.: #31893]

Pigment production

Information on culture and growth conditions Culture and growth conditions

[Ref.: #16581]

Culture medium

CHOPPED MEAT MEDIUM WITH CARBOHYDRATES (DSMZ Medium 110)

[Ref.: #16581]

Culture medium growth

[Ref.: #16581]

Culture medium link

Medium recipe at MediaDive

[Ref.: #16581]

Culture medium composition

expand / minimize

[Ref.: #40014]

Culture medium

MEDIUM 6 - Columbia agar with 10 % horse blood

[Ref.: #40014]

Culture medium growth

[Ref.: #40014]

Culture medium composition

expand / minimize

[Ref.: #123550]

Culture medium

CIP Medium 6

[Ref.: #123550]

Culture medium growth

[Ref.: #123550]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #123550]

Culture medium

CIP Medium 45

[Ref.: #123550]

Culture medium growth

[Ref.: #123550]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #16581]	growth	37 °C
[Ref.: #31893]	growth	25-37 °C
[Ref.: #31893]	optimum	37 °C
[Ref.: #40014]	growth	30 °C
[Ref.: #58743]	growth	30-37 °C
[Ref.: #67770]	growth	37 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #16581]	Oxygen tolerance	anaerobe
[Ref.: #31893]	Oxygen tolerance	anaerobe
[Ref.: #58743]	Oxygen tolerance	anaerobe
[Ref.: #123550]	Oxygen tolerance	anaerobe

[Ref.: #31893]	Ability of spore formation	no
[Ref.: #69480]	Ability of spore formation	no Confidence in %92.472
[Ref.: #69481]	Ability of spore formation	no Confidence in %100

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #31893]	5-dehydro-D-gluconate ChEBI	+	carbon source
[Ref.: #31893]	arginine ChEBI	+	carbon source
[Ref.: #68380]	arginine ChEBI	-	hydrolysis	* from API rID32A
[Ref.: #68367]	cellobiose ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	D-glucose ChEBI	+	builds acid from	* from API 20A
[Ref.: #68367]	D-mannitol ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	D-mannose ChEBI	+	builds acid from	* from API 20A
[Ref.: #68380]	D-mannose ChEBI	-	fermentation	* from API rID32A
[Ref.: #68367]	D-xylose ChEBI	-	builds acid from	* from API 20A
[Ref.: #31893]	esculin ChEBI	+	hydrolysis
[Ref.: #68367]	esculin ChEBI	-	hydrolysis	* from API 20A
[Ref.: #68367]	gelatin ChEBI	-	hydrolysis	* from API 20A
[Ref.: #31893]	glucose ChEBI	+	carbon source
[Ref.: #68367]	glycerol ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	L-arabinose ChEBI	-	builds acid from	* from API 20A
[Ref.: #68380]	L-glutamate ChEBI	-	degradation	* from API rID32A
[Ref.: #68367]	L-rhamnose ChEBI	-	builds acid from	* from API 20A
[Ref.: #31893]	lactose ChEBI	+	carbon source
[Ref.: #68367]	lactose ChEBI	+	builds acid from	* from API 20A
[Ref.: #31893]	maltose ChEBI	+	carbon source
[Ref.: #68367]	maltose ChEBI	+	builds acid from	* from API 20A
[Ref.: #68367]	melezitose ChEBI	-	builds acid from	* from API 20A
[Ref.: #31893]	N-acetylglucosamine ChEBI	+	carbon source
[Ref.: #123550]	nitrate ChEBI	-	reduction
[Ref.: #68380]	nitrate ChEBI	-	reduction	* from API rID32A
[Ref.: #123550]	nitrite ChEBI	-	reduction
[Ref.: #68367]	raffinose ChEBI	-	builds acid from	* from API 20A
[Ref.: #68380]	raffinose ChEBI	-	fermentation	* from API rID32A
[Ref.: #31893]	ribose ChEBI	+	carbon source
[Ref.: #68367]	salicin ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	sorbitol ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	sucrose ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	trehalose ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	tryptophan ChEBI	-	energy source	* from API 20A, API rID32A
[Ref.: #68367]	urea ChEBI	-	hydrolysis	* from API 20A, API rID32A
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #123550]		indole ChEBI	no
[Ref.: #68367]		indole ChEBI	no	* from API 20A, API rID32A

	Physiological tests	Metabolite	Indole test
[Ref.: #68380]		indole ChEBI	-	* from API rID32A, API 20A

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68380]	alanine arylamidase	+	3.4.11.2	* from API rID32A
[Ref.: #123550]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #31893]	alkaline phosphatase	+	3.1.3.1
[Ref.: #68380]	alkaline phosphatase	+	3.1.3.1	* from API rID32A, API zym
[Ref.: #68380]	alpha-arabinosidase	-	3.2.1.55	* from API rID32A
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68380]	alpha-fucosidase	+	3.2.1.51	* from API rID32A, API zym
[Ref.: #31893]	alpha-galactosidase	+	3.2.1.22
[Ref.: #68380]	alpha-galactosidase	-	3.2.1.22	* from API rID32A, API zym
[Ref.: #68380]	alpha-glucosidase	+	3.2.1.20	* from API rID32A, API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68380]	arginine dihydrolase	-	3.5.3.6	* from API rID32A
[Ref.: #68380]	beta-galactosidase	+	3.2.1.23	* from API rID32A, API zym
[Ref.: #68380]	beta-Galactosidase 6-phosphate	-		* from API rID32A
[Ref.: #68380]	beta-glucosidase	-	3.2.1.21	* from API rID32A, API 20A, API zym
[Ref.: #68380]	beta-glucuronidase	-	3.2.1.31	* from API rID32A, API zym
[Ref.: #123550]	catalase	-	1.11.1.6
[Ref.: #68367]	catalase	-	1.11.1.6	* from API 20A
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68367]	gelatinase	-		* from API 20A
[Ref.: #68380]	glutamate decarboxylase	-	4.1.1.15	* from API rID32A
[Ref.: #68380]	glutamyl-glutamate arylamidase	+		* from API rID32A
[Ref.: #68380]	glycin arylamidase	-		* from API rID32A
[Ref.: #68380]	histidine arylamidase	-		* from API rID32A
[Ref.: #68380]	L-arginine arylamidase	-		* from API rID32A
[Ref.: #68380]	leucine arylamidase	-	3.4.11.1	* from API rID32A, API zym
[Ref.: #68380]	leucyl glycin arylamidase	+	3.4.11.1	* from API rID32A
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #123550]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68380]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API rID32A, API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #123550]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #123550]	oxidase	-
[Ref.: #68380]	phenylalanine arylamidase	-		* from API rID32A
[Ref.: #68380]	proline-arylamidase	-	3.4.11.5	* from API rID32A
[Ref.: #68380]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API rID32A
[Ref.: #68380]	serine arylamidase	-		* from API rID32A
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68380]	tryptophan deaminase	-	4.1.99.1	* from API rID32A
[Ref.: #68380]	tyrosine arylamidase	-		* from API rID32A
[Ref.: #123550]	urease	-	3.5.1.5
[Ref.: #68380]	urease	-	3.5.1.5	* from API rID32A, API 20A
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 20A:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Indole productionIND	Urease/urea hydrolysisURE	Acid from D-glucoseGLU	Acid from D-mannitolMAN	Acid from lactoseLAC	Acid from sucroseSAC	Acid from maltoseMAL	Acid from salicinSAL	Acid from D-xyloseXYL	Acid from L-arabinoseARA	Gelatinase/gelatin hydrolysisGEL	Esculin hydrolysis/beta-glucosidaseESC	Acid from glycerolGLY	Acid from cellobioseCEL	Acid from D-mannoseMNE	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from sorbitolSOR	Acid from L-rhamnoseRHA	Acid from trehaloseTRE	CatalaseCAT	Spores presentSPOR	GramGRAM	Morphology coccus="+" rod="-"COCC
[Ref.: #16581]	2073	-	-	+	-	+	-	+	-	-	-	-	-	-	-	+	-	-	-	-	-	-	not determinedn.d.	not determinedn.d.	not determinedn.d.

API® rID32A:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Urease/urea hydrolysisURE	Arginine dihydrolaseADH (Arg)	alpha-Galactosidasealpha GAL	beta-Galactosidasebeta GAL	beta-Galactosidase 6-phosphatebeta GP	alpha-Glucosidasealpha GLU	beta-Glucosidasebeta GLU	alpha-Arabinosidasealpha ARA	beta-Glucuronidasebeta GUR	beta-N-Acetyl-beta-glucosaminidasebeta NAG	Fermentation of D-mannoseMNE	Fermentation of raffinoseRAF	Glutamate decarboxylaseGDC	alpha-Fucosidasealpha FUC	Reduction of nitrateNIT	Indole productionIND	Alkaline phosphatasePAL	L-arginine arylamidaseArgA	Proline arylamidaseProA	Leucyl glycin arylamidaseLGA	Phenylalanine arylamidasePheA	Leucine arylamidaseLeuA	Pyrrolidonyl arylamidasePyrA	Tyrosine arylamidaseTyrA	Alanine arylamidaseAlaA	Glycin arylamidaseGlyA	Histidine arylamidaseHisA	Glutamyl-glutamate arylamidaseGGA	Serine arylamidaseSerA
[Ref.: #16581]	12416	-	-	-	+	-	+	-	-	-	+	-	-	-	+	-	-	+	-	-	+	-	-	-	-	+	-	-	+	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #123550]	11253	-	+	+	+	-	-	-	-	-	-	+	+	-	+	-	+	-	+	-	+

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #16581]	Sample type/isolated from	human breast abscess (40-year-old woman)
[Ref.: #16581]	Geographic location (country and/or sea, region)	Marseille
[Ref.: #16581]	Country	France
[Ref.: #16581]	Country ISO 3 Code	FRA
[Ref.: #16581]	Continent	Europe

[Ref.: #58743]	Sample type/isolated from	Human abscess,breast,40-yr-old woman
[Ref.: #58743]	Sampling date	2004-06-01
[Ref.: #58743]	Geographic location (country and/or sea, region)	Marseille
[Ref.: #58743]	Country	France
[Ref.: #58743]	Country ISO 3 Code	FRA
[Ref.: #58743]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Human breast abscess

[Ref.: #123550]	Sample type/isolated from	Human, Mammary abscess
[Ref.: #123550]	Geographic location (country and/or sea, region)	Marseille
[Ref.: #123550]	Country	France
[Ref.: #123550]	Country ISO 3 Code	FRA
[Ref.: #123550]	Continent	Europe
[Ref.: #123550]	Isolation date	2004
* marker position based on {}

Isolation sources categories

#Host	#Human	#Female
#Host Body-Site	-	-
#Host Body-Site	#Other	#Abscess

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AB547706 (>99% sequence identity) for Prevotella timonensis subclade from Microbeatlas

Aquatic Samples	2334
Soil Samples	1601
Animal Samples	98467
Plant Samples	689
Total Samples	103091

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #16581]	Pathogenicity (human)	yes, in single cases
[Ref.: #16581]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #123550]	Biosafety level	2	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #16581]	Prevotella timonensis strain 4401737 16S ribosomal RNA gene, partial sequence	DQ518919	1454	GenBank ENA	1122992 tax ID
[Ref.: #20218]	Prevotella timonensis gene for 16S ribosomal RNA, partial sequence, strain: JCM 15640	AB547706	1493	GenBank ENA	1122992 tax ID	*

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Prevotella timonensis 4401737, DSM 22865	2523533627	draft	JGI IMG/M	1122992 tax ID	*
[Ref.: #66792]	Hoylesella timonensis 4401737 = DSM 22865 = JCM 15640	GCA_000430565	scaffold	GenBank ENA	1122992 tax ID	*
[Ref.: #66792]	Hoylesella timonensis 4401737 = DSM 22865 = JCM 15640	GCA_000614005	contig	GenBank ENA	1122992 tax ID	*
[Ref.: #66792]	Prevotella timonensis 4401737 = DSM 22865 = JCM 15640	1122992.3	wgs	PATRIC	1122992 tax ID	*
[Ref.: #66792]	Prevotella timonensis 4401737 = DSM 22865 = JCM 15640	1122992.4	wgs	PATRIC	1122992 tax ID	*
[Ref.: #66792]	Prevotella timonensis 4401737 = DSM 22865 = JCM 15640	1122992.6	wgs	PATRIC	1122992 tax ID	*

[Ref.: #16581]

GC-content

42.4 mol%

sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Prevotella timonensis 4401737 = DSM 22865 = JCM 15640 DSM 22865 NCBI: GCA_000430565.1
[Ref.: #69481]	spore-forming	spore-formingⓘ	no	100	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Prevotella timonensis 4401737 = DSM 22865 = JCM 15640 4401737 NCBI: GCA_000455445.1
[Ref.: #69480]	gram-positive	gram-positiveⓘ	no	94.779	yes
[Ref.: #69480]	anaerobic	anaerobicⓘ	yes	89.668	yes
[Ref.: #69480]	aerobic	aerobicⓘ	no	96.971	no
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	92.472	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	94.101	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	no	91.5	no

Availability in culture collections External links

[Ref.: #16581]

Culture collection no.

JCM 50105, DSM 22865, CCUG 50105, CIP 108522, JCM 15640, KCTC 15080

[Ref.: #81770]

SI-ID 302772

Literature:

Topic	Title	Authors	Journal	DOI	Year
Genetics	Non-contiguous finished genome sequence of Prevotella timonensis type strain 4401737(T.).	Roux V, Robert C, Raoult D	Stand Genomic Sci	10.4056/sigs.5098948	2014	*
Phylogeny	Prevotella timonensis sp. nov., isolated from a human breast abscess.	Glazunova OO, Launay T, Raoult D, Roux V	Int J Syst Evol Microbiol	10.1099/ijs.0.64609-0	2007	*

References

#16581

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 22865

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#31893

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #28152 (see below)

#40014 ; Curators of the CIP;
#58743 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 50105

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68367

Automatically annotated from API 20A .

#68380

Automatically annotated from API rID32A .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#81770

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID302772.1 )

#123550 Collection of Institut Pasteur ; Curators of the CIP; CIP 108522

#28152

IJSEM 883 2007 ( DOI 10.1099/ijs.0.64609-0 , PubMed 17392225 )

* These data were automatically processed and therefore are not curated

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Prevotella timonensis 4401737 = DSM 22865 = JCM 15640 DSM 22865

Prevotella timonensis 4401737 = DSM 22865 = JCM 15640 4401737

Change proposal

Change proposal