Name: Hoylesella loescheii DSM 19665
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 12553

Type strain:

Species: Hoylesella loescheii

Culture col. no.: DSM 19665, ATCC 15930, JCM 12249, NCTC 11321, CCUG 5914, Loesche strain 8B, JCM 8530, BCRC 10621, VPI 9085

Strain history: Tokyo Med. Dent. Univ. <-- Tama Biochem. Co., Ltd. <-- ATCC 15930 <-- W. J. Loesche 8B (Bacteroides oralis).

NCBI tax ID(s): 1122985 (strain), 840 (species)

SI-ID 92360

ATCC 15930, NCTC 11321, CCUG 5914, JCM 8530, CCRC 10621, KCTC 3691, BCRC 10621, JCM 12249, KCTC 5076, DSM 19665

Section

Hoylesella loescheii DSM 19665 is an anaerobe, mesophilic bacterium that was isolated from gingival crevice.

anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacteroidota*
	Class	*Bacteroidia*
	Order	*Bacteroidales*
	Family	*Prevotellaceae*
	Genus	*Hoylesella*
	Species	**Hoylesella loescheii**
	Full Scientific Name (LPSN)	Hoylesella loescheii (Holdeman and Johnson 1982) Hitch et al. 2023

	Synonym	Prevotella loescheii
	Synonym	Bacteroides loescheii

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	negative Confidence in %99.997

[Ref.: #69480]	Motility	no Confidence in %93.975

Information on culture and growth conditions Culture and growth conditions

[Ref.: #8242]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #8242]

Culture medium growth

[Ref.: #8242]

Culture medium link

Medium recipe at MediaDive

[Ref.: #8242]

Culture medium composition

expand / minimize

[Ref.: #8242]

Culture medium

PYG MEDIUM (MODIFIED) (DSMZ Medium 104)

[Ref.: #8242]

Culture medium growth

[Ref.: #8242]

Culture medium link

Medium recipe at MediaDive

[Ref.: #8242]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #8242]	growth	37 °C
[Ref.: #44876]	growth	37 °C
[Ref.: #67770]	growth	37 °C

[Ref.: #8242]	Temperature range	mesophilic
[Ref.: #44876]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #8242]	Oxygen tolerance	anaerobe
[Ref.: #44876]	Oxygen tolerance	anaerobe
[Ref.: #69480]	Oxygen tolerance	anaerobe Confidence in %98.651

[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.996

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68380]	arginine ChEBI	-	hydrolysis	* from API rID32A
[Ref.: #68380]	D-mannose ChEBI	+	fermentation	* from API rID32A
[Ref.: #68380]	L-glutamate ChEBI	-	degradation	* from API rID32A
[Ref.: #68380]	nitrate ChEBI	-	reduction	* from API rID32A
[Ref.: #68380]	raffinose ChEBI	+	fermentation	* from API rID32A
[Ref.: #68380]	tryptophan ChEBI	-	energy source	* from API rID32A
[Ref.: #68380]	urea ChEBI	-	hydrolysis	* from API rID32A

	Metabolite production	Metabolite	Production
[Ref.: #68380]		indole ChEBI	no	* from API rID32A

	Physiological tests	Metabolite	Indole test
[Ref.: #68380]		indole ChEBI	-	* from API rID32A

	Enzyme	Enzyme activity	EC number
[Ref.: #68380]	alanine arylamidase	+	3.4.11.2	* from API rID32A
[Ref.: #68380]	alkaline phosphatase	+	3.1.3.1	* from API rID32A
[Ref.: #68380]	alpha-arabinosidase	-	3.2.1.55	* from API rID32A
[Ref.: #68380]	alpha-fucosidase	+	3.2.1.51	* from API rID32A
[Ref.: #68380]	alpha-galactosidase	+	3.2.1.22	* from API rID32A
[Ref.: #68380]	alpha-glucosidase	+	3.2.1.20	* from API rID32A
[Ref.: #68380]	arginine dihydrolase	-	3.5.3.6	* from API rID32A
[Ref.: #68380]	beta-galactosidase	+	3.2.1.23	* from API rID32A
[Ref.: #68380]	beta-Galactosidase 6-phosphate	-		* from API rID32A
[Ref.: #68380]	beta-glucosidase	+	3.2.1.21	* from API rID32A
[Ref.: #68380]	beta-glucuronidase	-	3.2.1.31	* from API rID32A
[Ref.: #68380]	glutamate decarboxylase	-	4.1.1.15	* from API rID32A
[Ref.: #68380]	glutamyl-glutamate arylamidase	+		* from API rID32A
[Ref.: #68380]	glycin arylamidase	-		* from API rID32A
[Ref.: #68380]	histidine arylamidase	-		* from API rID32A
[Ref.: #68380]	L-arginine arylamidase	-		* from API rID32A
[Ref.: #68380]	leucine arylamidase	-	3.4.11.1	* from API rID32A
[Ref.: #68380]	leucyl glycin arylamidase	+	3.4.11.1	* from API rID32A
[Ref.: #68380]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API rID32A
[Ref.: #68380]	phenylalanine arylamidase	-		* from API rID32A
[Ref.: #68380]	proline-arylamidase	-	3.4.11.5	* from API rID32A
[Ref.: #68380]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API rID32A
[Ref.: #68380]	serine arylamidase	-		* from API rID32A
[Ref.: #68380]	tryptophan deaminase	-	4.1.99.1	* from API rID32A
[Ref.: #68380]	tyrosine arylamidase	-		* from API rID32A
[Ref.: #68380]	urease	-	3.5.1.5	* from API rID32A
	Only first 10 entries are displayed. Click here to see all.

API® rID32A:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Urease/urea hydrolysisURE	Arginine dihydrolaseADH (Arg)	alpha-Galactosidasealpha GAL	beta-Galactosidasebeta GAL	beta-Galactosidase 6-phosphatebeta GP	alpha-Glucosidasealpha GLU	beta-Glucosidasebeta GLU	alpha-Arabinosidasealpha ARA	beta-Glucuronidasebeta GUR	beta-N-Acetyl-beta-glucosaminidasebeta NAG	Fermentation of D-mannoseMNE	Fermentation of raffinoseRAF	Glutamate decarboxylaseGDC	alpha-Fucosidasealpha FUC	Reduction of nitrateNIT	Indole productionIND	Alkaline phosphatasePAL	L-arginine arylamidaseArgA	Proline arylamidaseProA	Leucyl glycin arylamidaseLGA	Phenylalanine arylamidasePheA	Leucine arylamidaseLeuA	Pyrrolidonyl arylamidasePyrA	Tyrosine arylamidaseTyrA	Alanine arylamidaseAlaA	Glycin arylamidaseGlyA	Histidine arylamidaseHisA	Glutamyl-glutamate arylamidaseGGA	Serine arylamidaseSerA
[Ref.: #8242]	12289	-	-	+	+	-	+	+	-	-	+	+	+	-	+	-	-	+	-	-	+	-	-	-	-	+	-	-	+	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #8242]	Sample type/isolated from	gingival crevice

[Ref.: #44876]	Sample type/isolated from	Gingival crevice

[Ref.: #67770]	Sample type/isolated from	Gingival crevice
* marker position based on {}

Isolation sources categories

#Host Body-Site

#Oral cavity and airways

#Gingiva

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AB547689 (>99% sequence identity) for Prevotella loescheii subclade from Microbeatlas

Aquatic Samples	404
Soil Samples	377
Animal Samples	26967
Plant Samples	102
Total Samples	27850

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #8242]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Prevotella loescheii gene for 16S ribosomal RNA, partial sequence, strain: JCM 8530	AB547689	1476	ENA	840 tax ID
[Ref.: #8242]	Prevotella loescheii ATCC 15930 16S ribosomal RNA gene, complete sequence	L16481	1480	ENA	840 tax ID
[Ref.: #20218]	Prevotella loescheii strain NCTC 11321 16S ribosomal RNA gene, partial sequence	AY836508	1451	ENA	840 tax ID	*
[Ref.: #20218]	Prevotella loescheii gene for 16S ribosomal RNA, partial sequence, strain: JCM 12249	AB547688	1490	ENA	840 tax ID	*

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Hoylesella loescheii DSM 19665 = JCM 12249 = ATCC 15930	GCA_000378085	scaffold	GenBank	1122985 tax ID
[Ref.: #67770]	Hoylesella loescheii DSM 19665 = JCM 12249 = ATCC 15930	GCA_000613485	contig	GenBank	1122985 tax ID
[Ref.: #67770]	Hoylesella loescheii DSM 19665 = JCM 12249 = ATCC 15930	GCA_000698925	scaffold	GenBank	1122985 tax ID
[Ref.: #66792]	Prevotella loescheii DSM 19665 = JCM 12249 = ATCC 15930	1122985.7	wgs	PATRIC	1122985 tax ID	*
[Ref.: #66792]	Prevotella loescheii DSM 19665 = JCM 12249 = ATCC 15930	1122985.3	wgs	PATRIC	1122985 tax ID	*
[Ref.: #66792]	Prevotella loescheii DSM 19665 = JCM 12249 = ATCC 15930	1122985.6	wgs	PATRIC	1122985 tax ID	*

[Ref.: #8242]

GC-content

46.6 mol%

sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Prevotella loescheii DSM 19665 = JCM 12249 = ATCC 15930 DSM 19665 NCBI: GCA_000378085.1
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Prevotella loescheii DSM 19665 = JCM 12249 = ATCC 15930 PATRIC: 1122985.7
[Ref.: #69480]	motile	no	92.497	no
[Ref.: #69480]	flagellated	no	96.634	no
[Ref.: #69480]	gram-positive	no	97.139	no
[Ref.: #69480]	anaerobic	yes	98.167	yes
[Ref.: #69480]	aerobic	no	98.077	no
[Ref.: #69480]	halophile	no	84.396	no
[Ref.: #69480]	spore-forming	no	95.723	no
[Ref.: #69480]	glucose-util	yes	83.117	no
[Ref.: #69480]	thermophile	no	99.072	yes
[Ref.: #69480]	glucose-ferment	yes	57.488	no

Availability in culture collections External links

[Ref.: #8242]

Culture collection no.

DSM 19665, ATCC 15930, JCM 12249, NCTC 11321, CCUG 5914, Loesche strain 8B, JCM 8530, BCRC 10621, VPI 9085

[Ref.: #81768]

SI-ID 92360

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Prevotella shahii sp. nov. and Prevotella salivae sp. nov., isolated from the human oral cavity.	Sakamoto M, Suzuki M, Huang Y, Umeda M, Ishikawa I, Benno Y	Int J Syst Evol Microbiol	10.1099/ijs.0.02876-0	2004	*
Metabolism	Inhibitory effect of lactoperoxidase-generated hypothiocyanite upon black pigmented anaerobe growth.	Fadel M, Courtois P	Int J Mol Med		2001	*
Enzymology	Isolation and characterisation of dipeptidyl peptidase IV from Prevotella loescheii ATCC 15930.	Koreeda Y, Hayakawa M, Ikemi T, Abiko Y	Arch Oral Biol	10.1016/s0003-9969(00)00065-0	2001	*
Metabolism	Fermentation product analysis in the identification of black-pigmented bacteria from periodontitis.	Takada K, Hirasawa M	Microbios		1997	*
Pathogenicity	Effect of periodontal treatments on serum IgG antibody titers against periodontopathic bacteria.	Horibe M, Watanabe H, Ishikawa I	J Clin Periodontol	10.1111/j.1600-051x.1995.tb00798.x	1995	*
Pathogenicity	Suppressive effect of soluble factor(s) derived from Prevotella loescheii ATCC 15930 on proliferation of human lymphocytes.	Terao N, Saito S, Hayakawa M, Abiko Y	Oral Microbiol Immunol	10.1111/j.1399-302x.1992.tb00030.x	1992	*
Enzymology	Purification and properties of neuraminidase from the culture medium of Bacteroides loescheii.	Takeshita T, Takagaki M, Amano A, Murakami Y, Nagata H, Shizukuishi S	Oral Microbiol Immunol	10.1111/j.1399-302x.1991.tb00501.x	1991	*
Enzymology	[Purification and some properties of neuraminidase from the culture medium of Bacteroides loescheii ATCC 15930].	Takeshita T	Osaka Daigaku Shigaku Zasshi		1989	*
Phylogeny	Immunoglobulin G response to subgingival gram-negative bacteria in human subjects.	Naito Y, Okuda K, Takazoe I	Infect Immun	10.1128/iai.45.1.47-51.1984	1984	*

References

#8242

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 19665

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#44876 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 5914

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68380

Automatically annotated from API rID32A .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#81768

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID92360.1 )

* These data were automatically processed and therefore are not curated

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Prevotella loescheii DSM 19665 = JCM 12249 = ATCC 15930 DSM 19665

Prevotella loescheii DSM 19665 = JCM 12249 = ATCC 15930

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