Name: Leyella stercorea CCUG 55595
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 12548

Type strain:

Species: Leyella stercorea

Culture col. no.: CCUG 55595, CB 35, DSM 18206, JCM 13469, KCTC 15079

Strain history: H. Hayashi CB35.

NCBI tax ID(s): 1002367 (strain), 363265 (species)

SI-ID 293996

JCM 13469, DSM 18206, CCUG 55595

Section

Leyella stercorea CCUG 55595 is an anaerobe, mesophilic, Gram-negative bacterium that was isolated from human faeces.

Gram-negative
rod-shaped
anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacteroidota*
	Class	*Bacteroidia*
	Order	*Bacteroidales*
	Family	*Prevotellaceae*
	Genus	*Leyella*
	Species	**Leyella stercorea**
	Full Scientific Name (LPSN)	Leyella stercorea (Hayashi et al. 2007) Hitch et al. 2023

Synonym

Prevotella stercorea

Information on morphological and physiological properties Morphology

[Ref.: #31995]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %100

[Ref.: #31995]	Cell length	1.16 µm

[Ref.: #31995]	Cell width	0.33 µm

[Ref.: #31995]	Cell shape	rod-shaped

[Ref.: #31995]	Motility	no

[Ref.: #7430]	Incubation period	3-7 days

[Ref.: #60661]	Incubation period	2 days

[Ref.: #31995]

Pigment production

Information on culture and growth conditions Culture and growth conditions

[Ref.: #7430]

Culture medium

FASTIDIOUS ANAEROBE BROTH (DSMZ Medium 1203a)

[Ref.: #7430]

Culture medium growth

[Ref.: #7430]

Culture medium link

Medium recipe at MediaDive

[Ref.: #7430]

Culture medium composition

expand / minimize

[Ref.: #7430]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #7430]

Culture medium growth

[Ref.: #7430]

Culture medium link

Medium recipe at MediaDive

[Ref.: #7430]

Culture medium composition

expand / minimize

[Ref.: #7430]

Culture medium

PYG MEDIUM (MODIFIED) (DSMZ Medium 104)

[Ref.: #7430]

Culture medium growth

[Ref.: #7430]

Culture medium link

Medium recipe at MediaDive

[Ref.: #7430]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #7430]	growth	37 °C
[Ref.: #31995]	optimum	37 °C
[Ref.: #60661]	growth	37 °C
[Ref.: #67770]	growth	37 °C

[Ref.: #7430]	Temperature range	mesophilic
[Ref.: #31995]	Temperature range	mesophilic
[Ref.: #60661]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #7430]	Oxygen tolerance	anaerobe
[Ref.: #31995]	Oxygen tolerance	anaerobe
[Ref.: #60661]	Oxygen tolerance	anaerobe
[Ref.: #69480]	Oxygen tolerance	anaerobe Confidence in %99.688

[Ref.: #31995]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.998

[Ref.: #67770]

Observation

quinones: MK-12, MK-13

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68380]	D-mannose ChEBI	-	fermentation	* from API rID32A
[Ref.: #31995]	glucose ChEBI	+	carbon source
[Ref.: #68380]	L-glutamate ChEBI	-	degradation	* from API rID32A
[Ref.: #31995]	lactose ChEBI	+	carbon source
[Ref.: #31995]	maltose ChEBI	+	carbon source
[Ref.: #31995]	mannose ChEBI	+	carbon source
[Ref.: #31995]	N-acetylglucosamine ChEBI	+	carbon source
[Ref.: #68380]	nitrate ChEBI	-	reduction	* from API rID32A
[Ref.: #31995]	raffinose ChEBI	+	carbon source
[Ref.: #31995]	sucrose ChEBI	+	carbon source
[Ref.: #68380]	tryptophan ChEBI	-	energy source	* from API rID32A
[Ref.: #68380]	urea ChEBI	-	hydrolysis	* from API rID32A
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #68380]		indole ChEBI	no	* from API rID32A

	Physiological tests	Metabolite	Indole test
[Ref.: #68380]		indole ChEBI	-	* from API rID32A

	Enzyme	Enzyme activity	EC number
[Ref.: #31995]	acid phosphatase	+	3.1.3.2
[Ref.: #68380]	alanine arylamidase	+	3.4.11.2	* from API rID32A
[Ref.: #31995]	alkaline phosphatase	+	3.1.3.1
[Ref.: #68380]	alpha-arabinosidase	-	3.2.1.55	* from API rID32A
[Ref.: #68380]	alpha-fucosidase	+	3.2.1.51	* from API rID32A
[Ref.: #68380]	alpha-galactosidase	+	3.2.1.22	* from API rID32A
[Ref.: #68380]	alpha-glucosidase	+	3.2.1.20	* from API rID32A
[Ref.: #68380]	beta-galactosidase	+	3.2.1.23	* from API rID32A
[Ref.: #68380]	beta-glucosidase	-	3.2.1.21	* from API rID32A
[Ref.: #68380]	beta-glucuronidase	-	3.2.1.31	* from API rID32A
[Ref.: #68380]	glutamate decarboxylase	-	4.1.1.15	* from API rID32A
[Ref.: #68380]	glycin arylamidase	-		* from API rID32A
[Ref.: #68380]	histidine arylamidase	-		* from API rID32A
[Ref.: #68380]	L-arginine arylamidase	-		* from API rID32A
[Ref.: #68380]	leucine arylamidase	-	3.4.11.1	* from API rID32A
[Ref.: #68380]	leucyl glycin arylamidase	+	3.4.11.1	* from API rID32A
[Ref.: #68380]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API rID32A
[Ref.: #68380]	phenylalanine arylamidase	-		* from API rID32A
[Ref.: #68380]	proline-arylamidase	-	3.4.11.5	* from API rID32A
[Ref.: #68380]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API rID32A
[Ref.: #68380]	serine arylamidase	-		* from API rID32A
[Ref.: #68380]	tryptophan deaminase	-	4.1.99.1	* from API rID32A
[Ref.: #68380]	tyrosine arylamidase	-		* from API rID32A
[Ref.: #68380]	urease	-	3.5.1.5	* from API rID32A
	Only first 10 entries are displayed. Click here to see all.

API® rID32A:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Urease/urea hydrolysisURE	Arginine dihydrolaseADH (Arg)	alpha-Galactosidasealpha GAL	beta-Galactosidasebeta GAL	beta-Galactosidase 6-phosphatebeta GP	alpha-Glucosidasealpha GLU	beta-Glucosidasebeta GLU	alpha-Arabinosidasealpha ARA	beta-Glucuronidasebeta GUR	beta-N-Acetyl-beta-glucosaminidasebeta NAG	Fermentation of D-mannoseMNE	Fermentation of raffinoseRAF	Glutamate decarboxylaseGDC	alpha-Fucosidasealpha FUC	Reduction of nitrateNIT	Indole productionIND	Alkaline phosphatasePAL	L-arginine arylamidaseArgA	Proline arylamidaseProA	Leucyl glycin arylamidaseLGA	Phenylalanine arylamidasePheA	Leucine arylamidaseLeuA	Pyrrolidonyl arylamidasePyrA	Tyrosine arylamidaseTyrA	Alanine arylamidaseAlaA	Glycin arylamidaseGlyA	Histidine arylamidaseHisA	Glutamyl-glutamate arylamidaseGGA	Serine arylamidaseSerA
[Ref.: #7430]	12243	-	-	+	+	+	+	-	-	-	+	-	-	-	+	-	-	+	-	-	+	-	-	-	-	+	-	-	-	-
[Ref.: #7430]	12242	-	+	+	+	-	+	-	-	-	+	-	+/-	-	+	-	-	+	-	-	+	-	-	-	-	+	-	-	-	-
[Ref.: #7430]	12241	-	-	+	+	-	+	-	-	-	+	-	-	-	+	-	-	-	-	-	+	-	-	-	-	+	-	-	+	-
[Ref.: #7430]	12240	-	-	+	+	-	+	-	-	-	+	-	-	-	+	-	-	+	-	-	+	-	-	-	-	+	-	-	+	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #7430]	Sample type/isolated from	human faeces
[Ref.: #7430]	Country	Japan
[Ref.: #7430]	Country ISO 3 Code	JPN
[Ref.: #7430]	Continent	Asia

[Ref.: #60661]	Sample type/isolated from	Human feces,52-yr-old healthy man
[Ref.: #60661]	Country	Japan
[Ref.: #60661]	Country ISO 3 Code	JPN
[Ref.: #60661]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Human feces
* marker position based on {}

Isolation sources categories

#Host	#Human	-
#Host Body Product	#Gastrointestinal tract	#Feces (Stool)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AB244774 (>99% sequence identity) for Prevotella stercorea subclade from Microbeatlas

Aquatic Samples	437
Soil Samples	191
Animal Samples	21622
Plant Samples	63
Total Samples	22313

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #7430]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #7430]	Prevotella stercorea gene for 16S ribosomal RNA, partial sequence, strain: CB35	AB244774	1488	ENA	1002367 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Leyella stercorea DSM 18206	GCA_000235885	scaffold	GenBank	1002367 tax ID
[Ref.: #66792]	Prevotella stercorea DSM 18206	1002367.3	wgs	PATRIC	1002367 tax ID	*
[Ref.: #66792]	Prevotella stercorea DSM 18206	2513237318	draft	JGI IMG/M	1002367 tax ID	*

[Ref.: #7430]	GC-content	48.2 mol%
[Ref.: #67770]	GC-content	48.2 mol%	high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Prevotella stercorea DSM 18206 DSM 18206 NCBI: GCA_000235885.1
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Prevotella stercorea DSM 18206 PATRIC: 1002367.3
[Ref.: #69480]	motile	no	90.735	no
[Ref.: #69480]	flagellated	no	96.89	yes
[Ref.: #69480]	gram-positive	no	96.906	yes
[Ref.: #69480]	anaerobic	yes	98.965	yes
[Ref.: #69480]	aerobic	no	97.731	yes
[Ref.: #69480]	halophile	no	89.038	no
[Ref.: #69480]	spore-forming	no	96.871	yes
[Ref.: #69480]	thermophile	no	97.361	yes
[Ref.: #69480]	glucose-util	yes	86.201	yes
[Ref.: #69480]	glucose-ferment	yes	59.511	no

Availability in culture collections External links

[Ref.: #7430]

Culture collection no.

CCUG 55595, CB 35, DSM 18206, JCM 13469, KCTC 15079

[Ref.: #81763]

SI-ID 293996

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Prevotella copri sp. nov. and Prevotella stercorea sp. nov., isolated from human faeces.	Hayashi H, Shibata K, Sakamoto M, Tomita S, Benno Y	Int J Syst Evol Microbiol	10.1099/ijs.0.64778-0	2007	*
Phylogeny	Prevotella merdae sp. nov., a new bacterial species isolated from human faeces.	Maaloum M, Afouda P, Lo CI, Dubourg G, Nguyen TT, Levasseur A, Saile R, Raoult D, Fournier PE	FEMS Microbiol Lett	10.1093/femsle/fnac066	2022	*

References

#7430

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 18206

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#31995

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #28249 (see below)

#60661 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 55595

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68380

Automatically annotated from API rID32A .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#81763

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID293996.1 )

#28249

IJSEM 941 2007 ( DOI 10.1099/ijs.0.64778-0 , PubMed 17473237 )

* These data were automatically processed and therefore are not curated

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Prevotella stercorea DSM 18206 DSM 18206

Prevotella stercorea DSM 18206

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