Prevotella multisaccharivorax (DSM 17128, JCM 12954, CCUG 58057)

Name: Prevotella multisaccharivorax (DSM 17128, JCM 12954, CCUG 58057)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 12543

Type strain

Strain Designation PPPA20

Culture col. no. DSM 17128 JCM 12954 CCUG 58057 PPPA20 KCTC 15700

NCBI tax ID(s) 688246 310514

Special Collections DSMZ CCUG JCM KCTC Literature strains

Links

Prevotella multisaccharivorax PPPA20 is an anaerobe, mesophilic, Gram-negative prokaryote that was isolated from subgingival plaque, chronic periodontitis.

Gram-negative coccus-shaped anaerobe mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 138338

JCM 12954,DSM 17128,CCUG 54633

@ref 20215

Last LPSN update 2025-12-16 09:49:52

Domain Pseudomonadati

Phylum Bacteroidota

Class Bacteroidia

Order Bacteroidales

Family Prevotellaceae

Genus Prevotella

Species Prevotella multisaccharivorax

Full scientific name Prevotella multisaccharivorax Sakamoto et al. 2005

Synonyms (1)

Hallella multisaccharivorax

BacDive ID	Other strains from **Prevotella multisaccharivorax** (5)	Type strain
161284	P. multisaccharivorax JCM 12955
161285	P. multisaccharivorax JCM 12956
161286	P. multisaccharivorax JCM 12957
161287	P. multisaccharivorax JCM 12958
161288	P. multisaccharivorax JCM 12959

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape
31524	negative	5.4 µm	0.8 µm	coccus-shaped
125438	negative
125439	negative

Pigmentation

31524

Productionno

Multimedia

@ref:	66793
multimedia content:	EM_DSM_17128_1.jpg
multimedia.multimedia content:	EM_DSM_17128_1.jpg
caption:	electron microscopic image
intellectual property rights:	© HZI/Manfred Rohde
manual_annotation:	1

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
6775	PYG MEDIUM (MODIFIED) (DSMZ Medium 104)		Medium recipe at MediaDive	Name: PYG MEDIUM (modified) (DSMZ Medium 104) Composition: Yeast extract 10.0 g/l Peptone 5.0 g/l Trypticase peptone 5.0 g/l Beef extract 5.0 g/l Glucose 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Tween 80 Vitamin K1 NaOH Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
6775	positive	growth	37	mesophilic
67770	positive	growth	37	mesophilic

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance
6775	anaerobe
31524	anaerobe
125439	anaerobe

Spore formation

@ref	Spore formation
31524
125439

Observation

@ref	Observation
31524	aggregates in clumps
67770	quinones: MK-12, MK-13

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
31524	22599 ChEBI	arabinose	+	carbon source
68380	29016 ChEBI	arginine	-	hydrolysis	from API rID32A
31524	17057 ChEBI	cellobiose	+	carbon source
31524	4853 ChEBI	esculin	+	hydrolysis
31524	17234 ChEBI	glucose	+	carbon source
68380	29985 ChEBI	L-glutamate	-	degradation	from API rID32A
31524	17716 ChEBI	lactose	+	carbon source
31524	17306 ChEBI	maltose	+	carbon source
31524	29864 ChEBI	mannitol	+	carbon source
31524	37684 ChEBI	mannose	+	carbon source
68380	17632 ChEBI	nitrate	-	reduction	from API rID32A
31524	16634 ChEBI	raffinose	+	carbon source
31524	26546 ChEBI	rhamnose	+	carbon source
31524	17814 ChEBI	salicin	+	carbon source
31524	30911 ChEBI	sorbitol	+	carbon source
31524	17992 ChEBI	sucrose	+	carbon source
31524	27082 ChEBI	trehalose	+	carbon source
68380	27897 ChEBI	tryptophan	-	energy source	from API rID32A
68380	16199 ChEBI	urea	-	hydrolysis	from API rID32A
31524	18222 ChEBI	xylose	+	carbon source

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68380	35581 ChEBI	indole		from API rID32A

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test
68380	35581 ChEBI	indole	-	from API rID32A

Enzymes

@ref	Value	Activity	Ec
68380	alanine arylamidase	+	3.4.11.2	from API rID32A
68380	alkaline phosphatase	+	3.1.3.1	from API rID32A
68380	alpha-arabinosidase	+	3.2.1.55	from API rID32A
68380	alpha-glucosidase	+	3.2.1.20	from API rID32A
68380	arginine dihydrolase	-	3.5.3.6	from API rID32A
68380	beta-galactosidase	+	3.2.1.23	from API rID32A
68380	beta-Galactosidase 6-phosphate	-		from API rID32A
68380	beta-glucuronidase	-	3.2.1.31	from API rID32A
68380	glutamate decarboxylase	-	4.1.1.15	from API rID32A
68380	glutamyl-glutamate arylamidase	-		from API rID32A
68380	glycin arylamidase	-		from API rID32A
68380	histidine arylamidase	-		from API rID32A
68380	L-arginine arylamidase	-		from API rID32A
68380	leucine arylamidase	-	3.4.11.1	from API rID32A
68380	leucyl glycin arylamidase	+	3.4.11.1	from API rID32A
68380	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API rID32A
68380	phenylalanine arylamidase	-		from API rID32A
68380	proline-arylamidase	-	3.4.11.5	from API rID32A
68380	pyrrolidonyl arylamidase	-	3.4.19.3	from API rID32A
68380	serine arylamidase	-		from API rID32A
68380	tryptophan deaminase	-	4.1.99.1	from API rID32A
68380	tyrosine arylamidase	-		from API rID32A
68380	urease	-	3.5.1.5	from API rID32A

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	cis-vaccenate biosynthesis	100	2 of 2
66794	glycogen metabolism	100	5 of 5
66794	C4 and CAM-carbon fixation	100	8 of 8
66794	ppGpp biosynthesis	100	4 of 4
66794	CMP-KDO biosynthesis	100	4 of 4
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	folate polyglutamylation	100	1 of 1
66794	palmitate biosynthesis	90.91	20 of 22
66794	starch degradation	90	9 of 10
66794	d-mannose degradation	88.89	8 of 9
66794	lipid A biosynthesis	88.89	8 of 9
66794	gluconeogenesis	87.5	7 of 8
66794	peptidoglycan biosynthesis	80	12 of 15
66794	vitamin K metabolism	80	4 of 5
66794	photosynthesis	78.57	11 of 14
66794	chorismate metabolism	77.78	7 of 9
66794	valine metabolism	77.78	7 of 9
66794	glycogen biosynthesis	75	3 of 4
66794	acetate fermentation	75	3 of 4
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	75	6 of 8
66794	sulfopterin metabolism	75	3 of 4
66794	isoleucine metabolism	75	6 of 8
66794	NAD metabolism	72.22	13 of 18
66794	tetrahydrofolate metabolism	71.43	10 of 14
66794	cardiolipin biosynthesis	71.43	5 of 7
66794	glycolysis	70.59	12 of 17
66794	phenylalanine metabolism	69.23	9 of 13
66794	leucine metabolism	69.23	9 of 13
66794	acetoin degradation	66.67	2 of 3
66794	formaldehyde oxidation	66.67	2 of 3
66794	serine metabolism	66.67	6 of 9
66794	aspartate and asparagine metabolism	66.67	6 of 9
66794	octane oxidation	66.67	2 of 3
66794	purine metabolism	63.83	60 of 94
66794	vitamin B6 metabolism	63.64	7 of 11
66794	methionine metabolism	61.54	16 of 26
66794	hydrogen production	60	3 of 5
66794	phenylacetate degradation (aerobic)	60	3 of 5
66794	threonine metabolism	60	6 of 10
66794	ubiquinone biosynthesis	57.14	4 of 7
66794	citric acid cycle	57.14	8 of 14
66794	glutamate and glutamine metabolism	57.14	16 of 28
66794	CO2 fixation in Crenarchaeota	55.56	5 of 9
66794	pyrimidine metabolism	55.56	25 of 45
66794	isoprenoid biosynthesis	53.85	14 of 26
66794	flavin biosynthesis	53.33	8 of 15
66794	alanine metabolism	51.72	15 of 29
66794	myo-inositol biosynthesis	50	5 of 10
66794	kanosamine biosynthesis II	50	1 of 2
66794	adipate degradation	50	1 of 2
66794	dTDPLrhamnose biosynthesis	50	4 of 8
66794	glycolate and glyoxylate degradation	50	3 of 6
66794	non-pathway related	50	19 of 38
66794	coenzyme A metabolism	50	2 of 4
66794	phenylmercury acetate degradation	50	1 of 2
66794	dolichol and dolichyl phosphate biosynthesis	50	1 of 2
66794	ethanol fermentation	50	1 of 2
66794	suberin monomers biosynthesis	50	1 of 2
66794	cysteine metabolism	50	9 of 18
66794	lipid metabolism	48.39	15 of 31
66794	vitamin B1 metabolism	46.15	6 of 13
66794	d-xylose degradation	45.45	5 of 11
66794	pentose phosphate pathway	45.45	5 of 11
66794	metabolism of disaccharids	45.45	5 of 11
66794	proline metabolism	45.45	5 of 11
66794	histidine metabolism	44.83	13 of 29
66794	reductive acetyl coenzyme A pathway	42.86	3 of 7
66794	oxidative phosphorylation	42.86	39 of 91
66794	tryptophan metabolism	42.11	16 of 38
66794	arginine metabolism	41.67	10 of 24
66794	lysine metabolism	40.48	17 of 42
66794	coenzyme M biosynthesis	40	4 of 10
66794	lipoate biosynthesis	40	2 of 5
66794	propionate fermentation	40	4 of 10
66794	methylglyoxal degradation	40	2 of 5
66794	metabolism of amino sugars and derivatives	40	2 of 5
66794	arachidonate biosynthesis	40	2 of 5
66794	degradation of hexoses	38.89	7 of 18
66794	sulfate reduction	38.46	5 of 13
66794	ketogluconate metabolism	37.5	3 of 8
66794	acetyl CoA biosynthesis	33.33	1 of 3
66794	selenocysteine biosynthesis	33.33	2 of 6
66794	enterobactin biosynthesis	33.33	1 of 3
66794	cyanate degradation	33.33	1 of 3
66794	degradation of aromatic, nitrogen containing compounds	33.33	4 of 12
66794	IAA biosynthesis	33.33	1 of 3
66794	L-lactaldehyde degradation	33.33	1 of 3
66794	nitrate assimilation	33.33	3 of 9
66794	degradation of pentoses	32.14	9 of 28
66794	degradation of sugar alcohols	31.25	5 of 16
66794	urea cycle	30.77	4 of 13
66794	polyamine pathway	30.43	7 of 23
66794	Entner Doudoroff pathway	30	3 of 10
66794	glycine metabolism	30	3 of 10
66794	tyrosine metabolism	28.57	4 of 14
66794	propanol degradation	28.57	2 of 7
66794	dolichyl-diphosphooligosaccharide biosynthesis	27.27	3 of 11
66794	lactate fermentation	25	1 of 4
66794	biotin biosynthesis	25	1 of 4
66794	cyclohexanol degradation	25	1 of 4
66794	butanoate fermentation	25	1 of 4
66794	phenylpropanoid biosynthesis	23.08	3 of 13
66794	phosphatidylethanolamine bioynthesis	23.08	3 of 13
66794	molybdenum cofactor biosynthesis	22.22	2 of 9
66794	glutathione metabolism	21.43	3 of 14
66794	heme metabolism	21.43	3 of 14

API rID32A

@ref	URE	ADH (Arg)	alpha GAL	beta GAL	beta-Galactosidase 6-phosphatebeta GP	alpha GLU	beta GLU	alpha ARA	beta GUR	beta-N-Acetyl-beta-glucosaminidasebeta NAG	MNE	RAF	GDC	alpha FUC	Reduction of nitrateNIT	IND	PAL	L-arginine arylamidaseArgA	ProA	LGA	Phenylalanine arylamidasePheA	Leucine arylamidaseLeuA	PyrA	Tyrosine arylamidaseTyrA	Alanine arylamidaseAlaA	Glycin arylamidaseGlyA	Histidine arylamidaseHisA	Glutamyl-glutamate arylamidaseGGA	Serine arylamidaseSerA
6775	-	-	-	+	-	+	-	+	-	+	-	-	-	-	-	-	+	-	-	+	-	-	-	-	+	-	-	-	-
6775	-	-	-	+	-	+	-	+	-	+	-	-	-	-	-	-	+	-	-	+	-	-	-	-	+	-	-	-	-
6775	-	-	-	+	-	+	-	+	-	+	-	-	-	-	-	-	+	-	-	+	-	-	-	-	+	-	-	-	-
6775	-	-	+	+	-	+	+	+	-	+	+/-	+/-	-	+/-	-	-	+	-	-	+	-	-	-	-	+	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2
#Infection	#Inflammation
#Host Body-Site	#Oral cavity and airways

Isolation

@ref	Sample type	Country	Country ISO 3 Code	Continent
6775	subgingival plaque, chronic periodontitis	Japan	JPN	Asia
61578	Human subgingival plaque,chronic periodontitis	Japan	JPN	Asia
67770	Subgingival plaque of a patient with chronic periodontitis

Taxonmaps

69479

Global distribution of 16S sequence AB200414 (>99% sequence identity) for Prevotella multisaccharivorax subclade from Microbeatlas

Aquatic Samples	179
Soil Samples	71
Animal Samples	9226
Plant Samples	50
Total Samples	9526

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
6775	2	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM21823v1 assembly for Hallella multisaccharivorax DSM 17128	scaffold	GCA_000218235	688246.3	2503754046	688246	73.32
66792	Prevotella multisaccharivorax DSM 17128 strain JCM 12954	contig		688246.6		688246	73.2
66792	Prevotella multisaccharivorax DSM 17128 strain JCM 12954	contig		688246.9		688246	73.2
66792	Prevotella multisaccharivorax DSM 17128 strain JCM 12954	contig		688246.7		688246	73.2
66792	Prevotella multisaccharivorax DSM 17128 strain JCM 12954	contig		688246.8		688246	73.2
66792	ASM1997331v1 assembly for Hallella multisaccharivorax DSM 17128 JCM 12954	scaffold	GCA_019973315	688246.10	8112594425	688246	71.15

Genome browser: GCA_000218235

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
6775	Prevotella multisaccharivorax gene for 16S ribosomal RNA, partial sequence	AB200414	1491		688246

GC content

@ref	GC-content (mol%)
6775	49.9
6775	48.3
67770	49.9

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Hallella multisaccharivorax DSM 17128 JCM 12954
NCBI: GCA_019973315

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	99.80	no
125439	motility	BacteriaNetⓘ	yes	56.20	no
125439	gram_stain	BacteriaNetⓘ	negative	99.80	no
125439	oxygen_tolerance	BacteriaNetⓘ	anaerobe	92.10	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Prevotella multisaccharivorax DSM 17128 JCM 12954
NCBI: GCA_019973315.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	93.09	no
125438	anaerobic	anaerobicⓘ	yes	89.59	no
125438	aerobic	aerobicⓘ	no	96.25	no
125438	spore-forming	spore-formingⓘ	no	89.99	yes
125438	thermophilic	thermophileⓘ	no	91.63	yes
125438	flagellated	motile2+ⓘ	no	86.00	yes

Literature

Topic	Title	Authors	Journal	DOI	Year
Genetics	Non-contiguous finished genome sequence of the opportunistic oral pathogen Prevotella multisaccharivorax type strain (PPPA20).	Pati A, Gronow S, Lu M, Lapidus A, Nolan M, Lucas S, Hammon N, Deshpande S, Cheng JF, Tapia R, Han C, Goodwin L, Pitluck S, Liolios K, Pagani I, Mavromatis K, Mikhailova N, Huntemann M, Chen A, Palaniappan K, Land M, Hauser L, Detter JC, Brambilla EM, Rohde M, Goker M, Woyke T, Bristow J, Eisen JA, Markowitz V, Hugenholtz P, Kyrpides NC, Klenk HP, Ivanova N	Stand Genomic Sci	10.4056/sigs.2164949	2011
Phylogeny	Prevotella lacticifex sp. nov., isolated from the rumen of cows.	Shinkai T, Ikeyama N, Kumagai M, Ohmori H, Sakamoto M, Ohkuma M, Mitsumori M	Int J Syst Evol Microbiol	10.1099/ijsem.0.005278	2022
Phylogeny	Prevotella multisaccharivorax sp. nov., isolated from human subgingival plaque.	Sakamoto M, Umeda M, Ishikawa I, Benno Y	Int J Syst Evol Microbiol	10.1099/ijs.0.63739-0	2005

References

#6775	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 17128
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#31524	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27818 (see below)
#61578	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 58057
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#66793	Mukherjee et al.: GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life. 35: 676 - 683 2017 ( DOI 10.1038/nbt.3886 , PubMed 28604660 )
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68380	Automatically annotated from API rID32A .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Prevotella multisaccharivorax (DSM 17128, JCM 12954, CCUG 58057)

Name and taxonomic classification

Morphology

Cell morphology

Pigmentation

Multimedia

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Observation

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

Pathway annotation

API rID32A

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000218235

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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