Prevotella multiformis (CCUG 51937, DSM 16608, JCM 12541)

Name: Prevotella multiformis (CCUG 51937, DSM 16608, JCM 12541)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 12540

Type strain

Strain Designation PPPA21

Culture col. no. CCUG 51937 DSM 16608 JCM 12541 PPPA21

NCBI tax ID(s) 888743 282402

Special Collections DSMZ CCUG JCM Literature strains

History <- M. Sakamoto; PPPA21 M. Sakamoto PPPA21.

Links

Prevotella multiformis PPPA21 is an anaerobe, Gram-negative, rod-shaped bacterium that was isolated from subgingival plaque of a patient with chronic periodontitis.

Gram-negative rod-shaped anaerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 138019

DSM 16608,JCM 12541,CCUG 51937

@ref 20215

Last LPSN update 2026-02-09 11:20:51

Domain Bacteria

Phylum Bacteroidota

Class Bacteroidia

Order Bacteroidales

Family Prevotellaceae

Genus Prevotella

Species Prevotella multiformis

Full scientific name Prevotella multiformis Sakamoto et al. 2005

BacDive ID	Other strains from **Prevotella multiformis** (3)	Type strain
161248	P. multiformis JCM 12542
161249	P. multiformis JCM 12543
161250	P. multiformis JCM 12544

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Confidence
31388	negative	4.1 µm	0.65 µm	rod-shaped
68367				rod-shaped		from API 20A
68367	negative					from API 20A
125439	negative				99
125438	negative				95.213

Pigmentation

31388

Productionyes

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
6518	COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)		Medium recipe at MediaDive	Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
6518	PYG MEDIUM (MODIFIED) (DSMZ Medium 104)		Medium recipe at MediaDive	Name: PYG MEDIUM (modified) (DSMZ Medium 104) Composition: Yeast extract 10.0 g/l Peptone 5.0 g/l Trypticase peptone 5.0 g/l Beef extract 5.0 g/l Glucose 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Tween 80 Vitamin K1 NaOH Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
6518	positive	growth	37
59375	positive	growth	37
67770	positive	growth	37

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
6518	anaerobe
31388	anaerobe
59375	anaerobe
125438	anaerobe	91.911
125439	anaerobe	99

Spore formation

@ref	Confidence
31388
68367		from API 20A
125439	99.9
125438	92.472

Observation

67770

Observationquinones: MK-10, MK-11, MK-12, MK-13

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68380	29016 ChEBI	arginine	-	hydrolysis	from API rID32A
31388	17057 ChEBI	cellobiose	+	carbon source
68367	17057 ChEBI	cellobiose	+	builds acid from	from API 20A
68367	17634 ChEBI	D-glucose	+	builds acid from	from API 20A
68380	16024 ChEBI	D-mannose	+	fermentation	from API rID32A
68367	16024 ChEBI	D-mannose	+	builds acid from	from API 20A
68367	4853 ChEBI	esculin	-	hydrolysis	from API 20A
31388	17234 ChEBI	glucose	+	carbon source
31388	17754 ChEBI	glycerol	+	carbon source
68380	29985 ChEBI	L-glutamate	-	degradation	from API rID32A
31388	17716 ChEBI	lactose	+	carbon source
31388	17306 ChEBI	maltose	+	carbon source
68367	17306 ChEBI	maltose	+	builds acid from	from API 20A
31388	37684 ChEBI	mannose	+	carbon source
68380	17632 ChEBI	nitrate	-	reduction	from API rID32A
31388	16634 ChEBI	raffinose	+	carbon source
68380	16634 ChEBI	raffinose	+	fermentation	from API rID32A
68367	16634 ChEBI	raffinose	+	builds acid from	from API 20A
31388	17992 ChEBI	sucrose	+	carbon source
68367	17992 ChEBI	sucrose	+	builds acid from	from API 20A
68380	27897 ChEBI	tryptophan	-	energy source	from API rID32A
68367	27897 ChEBI	tryptophan	-	energy source	from API 20A
68380	16199 ChEBI	urea	-	hydrolysis	from API rID32A
68367	16199 ChEBI	urea	-	hydrolysis	from API 20A

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68380	35581 ChEBI	indole		from API rID32A
68367	35581 ChEBI	indole		from API 20A

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test
68380	35581 ChEBI	indole	-	from API rID32A
68367	35581 ChEBI	indole	-	from API 20A

Enzymes

@ref	Value	Activity	Ec
68380	alanine arylamidase	+	3.4.11.2	from API rID32A
68380	alkaline phosphatase	+	3.1.3.1	from API rID32A
68380	alpha-arabinosidase	-	3.2.1.55	from API rID32A
68380	alpha-fucosidase	+	3.2.1.51	from API rID32A
68380	alpha-glucosidase	+	3.2.1.20	from API rID32A
68380	arginine dihydrolase	-	3.5.3.6	from API rID32A
68380	beta-galactosidase	+	3.2.1.23	from API rID32A
68380	beta-Galactosidase 6-phosphate	+		from API rID32A
68367	beta-glucosidase	-	3.2.1.21	from API 20A
68380	beta-glucuronidase	-	3.2.1.31	from API rID32A
68367	catalase	-	1.11.1.6	from API 20A
68380	glutamate decarboxylase	-	4.1.1.15	from API rID32A
68380	glutamyl-glutamate arylamidase	+		from API rID32A
68380	glycin arylamidase	-		from API rID32A
68380	histidine arylamidase	-		from API rID32A
68380	L-arginine arylamidase	-		from API rID32A
68380	leucyl glycin arylamidase	+	3.4.11.1	from API rID32A
68380	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API rID32A
68380	phenylalanine arylamidase	-		from API rID32A
68380	proline-arylamidase	-	3.4.11.5	from API rID32A
68380	pyrrolidonyl arylamidase	-	3.4.19.3	from API rID32A
68380	serine arylamidase	-		from API rID32A
68380	tryptophan deaminase	-	4.1.99.1	from API rID32A
68380	tyrosine arylamidase	-		from API rID32A
68380	urease	-	3.5.1.5	from API rID32A
68367	urease	-	3.5.1.5	from API 20A

API 20A

@ref	IND	URE	GLU	MAN	LAC	SAC	MAL	SAL	XYL	ARA	GEL	ESC	GLY	CEL	MNE	MLZ	RAF	SOR	RHA	TRE	CAT	Spores presentSPOR	GramGRAM	Morphology coccus="+" rod="-"COCC
6518	-	-	+	-	+	+	+	-	-	-	+	-	+	+	+	-	+	-	-	-	-	-	-	-
6518	-	-	+	+	+/-	+	+	+/-	+/-	+/-	-	-	+/-	+	+	+/-	+	+/-	+/-	+/-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API rID32A

@ref	URE	ADH (Arg)	alpha GAL	beta GAL	beta-Galactosidase 6-phosphatebeta GP	alpha GLU	beta GLU	alpha ARA	beta GUR	beta-N-Acetyl-beta-glucosaminidasebeta NAG	MNE	RAF	GDC	alpha FUC	Reduction of nitrateNIT	IND	PAL	L-arginine arylamidaseArgA	ProA	LGA	Phenylalanine arylamidasePheA	Leucine arylamidaseLeuA	PyrA	Tyrosine arylamidaseTyrA	Alanine arylamidaseAlaA	Glycin arylamidaseGlyA	Histidine arylamidaseHisA	Glutamyl-glutamate arylamidaseGGA	Serine arylamidaseSerA
6518	-	-	+	+	+	+	+	-	-	+	+	+	-	+	-	-	+	-	-	+	-	+/-	-	-	+	-	-	+	-
6518	-	-	-	+	+	+	-	-	-	+	+	+	-	+	-	-	+	-	-	+	-	+/-	-	-	+	-	-	+	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Infection	#Inflammation	-
#Infection	#Patient	-
#Host Body-Site	#Oral cavity and airways	#Plaque

Isolation

@ref	Sample type	Country	Country ISO 3 Code	Continent
6518	subgingival plaque of a patient with chronic periodontitis	Japan	JPN	Asia
59375	Human subgingival plaque
67770	Human subgingival plaque

Taxonmaps

69479

Global distribution of 16S sequence AB182483 (>99% sequence identity) for Prevotella multiformis subclade from Microbeatlas

Aquatic Samples	396
Soil Samples	243
Animal Samples	19978
Plant Samples	113
Total Samples	20730

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
6518	2	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM19106v1 assembly for Prevotella multiformis DSM 16608	scaffold	GCA_000191065	888743.3	649989987	888743	65.32

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
6518	Prevotella multiformis gene for 16S rRNA, partial sequence	AB182483	1494		888743

GC content

@ref	GC-content (mol%)	Method
6518	51.1
67770	51.1	high performance liquid chromatography (HPLC)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Prevotella multiformis DSM 16608
NCBI: GCA_000191065

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	99.90	no
125439	motility	BacteriaNetⓘ	no	52.90	no
125439	gram_stain	BacteriaNetⓘ	negative	99.00	no
125439	oxygen_tolerance	BacteriaNetⓘ	anaerobe	99.00	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Prevotella multiformis DSM 16608
PATRIC: 888743.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	95.21	yes
125438	anaerobic	anaerobicⓘ	yes	91.91	yes
125438	aerobic	aerobicⓘ	no	97.37	yes
125438	spore-forming	spore-formingⓘ	no	92.47	yes
125438	thermophilic	thermophileⓘ	no	96.20	yes
125438	flagellated	motile2+ⓘ	no	89.00	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Growth of succinate consumer Dialister hominis is supported by Bacteroides thetaiotaomicron.	Sakamoto M, Ikeyama N, Iino T, Ohkuma M.	Anaerobe	10.1016/j.anaerobe.2022.102642	2022
Metabolism	Intra-Genomic Heterogeneity in 16S rRNA Genes in Strictly Anaerobic Clinical Isolates from Periodontal Abscesses.	Chen J, Miao X, Xu M, He J, Xie Y, Wu X, Chen G, Yu L, Zhang W.	PLoS One	10.1371/journal.pone.0130265	2015
Enzymology	Lactobacilli are prominent in the initial stages of polymicrobial infection of dental pulp.	Nadkarni MA, Simonian MR, Harty DW, Zoellner H, Jacques NA, Hunter N.	J Clin Microbiol	10.1128/jcm.01912-09	2010
Phylogeny	Prevotella dentasini sp. nov., a black-pigmented species isolated from the oral cavity of donkeys.	Takada K, Hayashi K, Sato Y, Hirasawa M	Int J Syst Evol Microbiol	10.1099/ijs.0.017020-0	2009
Phylogeny	Prevotella multiformis sp. nov., isolated from human subgingival plaque.	Sakamoto M, Huang Y, Umeda M, Ishikawa I, Benno Y	Int J Syst Evol Microbiol	10.1099/ijs.0.63451-0	2005

References

#6518	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 16608
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#27699	IJSEM 815 2005 ( DOI 10.1099/ijs.0.63451-0 , PubMed 15774668 )
#31388	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27699
#59375	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 51937
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68367	Automatically annotated from API 20A .
#68380	Automatically annotated from API rID32A .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Prevotella multiformis (CCUG 51937, DSM 16608, JCM 12541)

Name and taxonomic classification

Morphology

Cell morphology

Pigmentation

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Observation

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API 20A

API rID32A

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000191065

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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