Name: Segatella bryantii B14
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 12536

Type strain:

Species: Segatella bryantii

Strain Designation: B14, B₁4

Culture col. no.: DSM 11371, CIP 105474, JCM 13993

Strain history: CIP <- 1998, G. Avgustin, Univ. Ljubljana, Slovenia: strain B14 <- H.J. Flint, Rowett. Res. Inst., Aberdeen, Scotland <- J.B. Russell, Cornell Univ., Ithaca, N.Y., Bacteroides rumicola subsp. brevis

NCBI tax ID(s): 77095 (species)

SI-ID 49087

DSM 11371, CIP 105474, CCUG 52343, JCM 13993

Section

Segatella bryantii B14 is an anaerobe, mesophilic, Gram-negative bacterium that was isolated from rumen of cow.

Gram-negative
rod-shaped
anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacteroidota*
	Class	*Bacteroidia*
	Order	*Bacteroidales*
	Family	*Prevotellaceae*
	Genus	*Segatella*
	Species	**Segatella bryantii**
	Full Scientific Name (LPSN)	Segatella bryantii (Avguštin et al. 1997) Hitch et al. 2023

Synonym

Prevotella bryantii

Information on morphological and physiological properties Morphology

[Ref.: #117065]	Gram stain	negative

[Ref.: #117065]	Cell shape	rod-shaped

[Ref.: #117065]	Motility	no

Information on culture and growth conditions Culture and growth conditions

[Ref.: #4314]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #4314]

Culture medium growth

[Ref.: #4314]

Culture medium link

Medium recipe at MediaDive

[Ref.: #4314]

Culture medium composition

expand / minimize

[Ref.: #4314]

Culture medium

TREPONEMA MEDIUM (DSMZ Medium 159)

[Ref.: #4314]

Culture medium growth

[Ref.: #4314]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #40178]

Culture medium

MEDIUM 20 - for Anaerobic bacteria

[Ref.: #40178]

Culture medium growth

[Ref.: #40178]

Culture medium composition

expand / minimize

[Ref.: #117065]

Culture medium

CIP Medium 20

[Ref.: #117065]

Culture medium growth

[Ref.: #117065]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #4314]	growth	37 °C
[Ref.: #40178]	growth	37 °C
[Ref.: #117065]	growth	37 °C

[Ref.: #4314]	Temperature range	mesophilic
[Ref.: #40178]	Temperature range	mesophilic
[Ref.: #117065]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #4314]

Oxygen tolerance

anaerobe

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68380]	arginine ChEBI	-	hydrolysis	* from API rID32A
[Ref.: #68380]	D-mannose ChEBI	-	fermentation	* from API rID32A
[Ref.: #68380]	L-glutamate ChEBI	-	degradation	* from API rID32A
[Ref.: #68380]	nitrate ChEBI	-	reduction	* from API rID32A
[Ref.: #68380]	raffinose ChEBI	-	fermentation	* from API rID32A
[Ref.: #68380]	tryptophan ChEBI	-	energy source	* from API rID32A
[Ref.: #68380]	urea ChEBI	-	hydrolysis	* from API rID32A

	Metabolite production	Metabolite	Production
[Ref.: #68380]		indole ChEBI	no	* from API rID32A

	Physiological tests	Metabolite	Indole test
[Ref.: #68380]		indole ChEBI	-	* from API rID32A

	Enzyme	Enzyme activity	EC number
[Ref.: #68380]	alanine arylamidase	+	3.4.11.2	* from API rID32A
[Ref.: #68380]	alkaline phosphatase	+	3.1.3.1	* from API rID32A
[Ref.: #68380]	alpha-arabinosidase	+	3.2.1.55	* from API rID32A
[Ref.: #68380]	alpha-fucosidase	-	3.2.1.51	* from API rID32A
[Ref.: #68380]	arginine dihydrolase	-	3.5.3.6	* from API rID32A
[Ref.: #68380]	beta-glucosidase	+	3.2.1.21	* from API rID32A
[Ref.: #68380]	beta-glucuronidase	-	3.2.1.31	* from API rID32A
[Ref.: #68380]	glutamate decarboxylase	-	4.1.1.15	* from API rID32A
[Ref.: #68380]	glycin arylamidase	-		* from API rID32A
[Ref.: #68380]	histidine arylamidase	-		* from API rID32A
[Ref.: #68380]	L-arginine arylamidase	-		* from API rID32A
[Ref.: #68380]	leucine arylamidase	-	3.4.11.1	* from API rID32A
[Ref.: #68380]	leucyl glycin arylamidase	+	3.4.11.1	* from API rID32A
[Ref.: #68380]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API rID32A
[Ref.: #68380]	phenylalanine arylamidase	-		* from API rID32A
[Ref.: #68380]	proline-arylamidase	-	3.4.11.5	* from API rID32A
[Ref.: #68380]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API rID32A
[Ref.: #68380]	serine arylamidase	-		* from API rID32A
[Ref.: #68380]	tryptophan deaminase	-	4.1.99.1	* from API rID32A
[Ref.: #68380]	tyrosine arylamidase	-		* from API rID32A
[Ref.: #68380]	urease	-	3.5.1.5	* from API rID32A
	Only first 10 entries are displayed. Click here to see all.

API® rID32A:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Urease/urea hydrolysisURE	Arginine dihydrolaseADH (Arg)	alpha-Galactosidasealpha GAL	beta-Galactosidasebeta GAL	beta-Galactosidase 6-phosphatebeta GP	alpha-Glucosidasealpha GLU	beta-Glucosidasebeta GLU	alpha-Arabinosidasealpha ARA	beta-Glucuronidasebeta GUR	beta-N-Acetyl-beta-glucosaminidasebeta NAG	Fermentation of D-mannoseMNE	Fermentation of raffinoseRAF	Glutamate decarboxylaseGDC	alpha-Fucosidasealpha FUC	Reduction of nitrateNIT	Indole productionIND	Alkaline phosphatasePAL	L-arginine arylamidaseArgA	Proline arylamidaseProA	Leucyl glycin arylamidaseLGA	Phenylalanine arylamidasePheA	Leucine arylamidaseLeuA	Pyrrolidonyl arylamidasePyrA	Tyrosine arylamidaseTyrA	Alanine arylamidaseAlaA	Glycin arylamidaseGlyA	Histidine arylamidaseHisA	Glutamyl-glutamate arylamidaseGGA	Serine arylamidaseSerA
[Ref.: #4314]	13358	-	-	+/-	+	-	+	+	+	-	-	-	-	-	-	-	-	+	-	-	+	-	-	-	-	+	-	-	+/-	-
[Ref.: #4314]	13357	-	-	+	+	+/-	+	+	+	-	-	-	-	-	-	-	-	+	-	-	+	-	-	-	-	+	-	-	-	-
[Ref.: #4314]	12164	-	-	-	+	-	+/-	+	+	-	-	-	-	-	-	-	-	+	-	-	+	-	-	-	-	+	-	-	-	-
[Ref.: #4314]	12163	-	-	-	-	-	+	+	+	-	-	-	-	-	-	-	-	+	-	-	+	-	-	-	-	+	-	-	-	-
[Ref.: #4314]	12162	-	-	+	+	+/-	+	+	+	-	-	-	-	-	-	-	-	+	-	-	+	-	-	-	-	+	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #4314]	Sample type/isolated from	rumen of cow
[Ref.: #4314]	Country	USA
[Ref.: #4314]	Country ISO 3 Code	USA
[Ref.: #4314]	Continent	North America

[Ref.: #117065]	Sample type/isolated from	Animal, Cattle, rumen
[Ref.: #117065]	Country	United States of America
[Ref.: #117065]	Country ISO 3 Code	USA
[Ref.: #117065]	Continent	North America
[Ref.: #117065]	Isolation date	1958
* marker position based on {}

Isolation sources categories

#Host	#Mammals	#Bovinae (Cow, Cattle)
#Host Body-Site	#Organ	#Rumen
#Host Body-Site	#Gastrointestinal tract	#Stomach

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence NR_028866 (>99% sequence identity) for Prevotella bryantii subclade from Microbeatlas

Aquatic Samples	489
Soil Samples	406
Animal Samples	22190
Plant Samples	441
Total Samples	23526

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #4314]	Biosafety level	2	Risk group (German classification) According to TRBA 466
[Ref.: #117065]	Biosafety level	2	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #4314]	Prevotella bryantii B14 16S ribosomal RNA, partial sequence	NR_028866	1486	GenBank	752555 tax ID
[Ref.: #20218]	Prevotella bryantii 16S rRNA gene	AJ006457	1486	ENA	752555 tax ID	*

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Prevotella bryantii strain DSM 11371	77095.21	wgs	PATRIC	77095 tax ID	*
[Ref.: #66792]	Prevotella bryantii B14	GCA_900110745	contig	GenBank	752555 tax ID	*
[Ref.: #66792]	Prevotella bryantii B14	GCA_000179055	contig	GenBank	752555 tax ID	*
[Ref.: #66792]	Prevotella bryantii B14	752555.7	wgs	PATRIC	752555 tax ID	*
[Ref.: #66792]	Prevotella bryantii B14	752555.5	wgs	PATRIC	752555 tax ID	*
[Ref.: #66792]	Prevotella bryantii strain DSM 11371	77095.19	wgs	PATRIC	77095 tax ID	*

[Ref.: #4314]

GC-content

39.1 mol%

sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Prevotella bryantii B14 B14 NCBI: GCA_022024235.1
[Ref.: #69480]	flagellated	no	94.867	no
[Ref.: #69480]	gram-positive	no	97.481	no
[Ref.: #69480]	anaerobic	yes	99.376	yes
[Ref.: #69480]	aerobic	no	98.535	yes
[Ref.: #69480]	halophile	no	89.29	no
[Ref.: #69480]	spore-forming	no	95.922	no
[Ref.: #69480]	motile	no	91.076	no
[Ref.: #69480]	glucose-ferment	yes	55.159	no
[Ref.: #69480]	thermophile	no	96.571	yes
[Ref.: #69480]	glucose-util	yes	82.686	no

Availability in culture collections External links

[Ref.: #4314]

Culture collection no.

DSM 11371, CIP 105474, JCM 13993

[Ref.: #81753]

SI-ID 49087

References

#4314

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 11371

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#40178 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68380

Automatically annotated from API rID32A .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#81753

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID49087.1 )

#117065 Collection of Institut Pasteur ; Curators of the CIP; CIP 105474
* These data were automatically processed and therefore are not curated

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Prevotella bryantii B14 B14

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