Name: Hoylesella buccalis HS4
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 12528

Type strain:

Species: Hoylesella buccalis

Strain Designation: HS4

Culture col. no.: CCUG 15557, NCTC 13064, JCM 12246, DSM 20616, ATCC 35310, NCDO 2354

Strain history: CCUG 15557 <-- NCDO 2354 <-- H. N. Shah HS4.

NCBI tax ID(s): 679190 (strain), 28127 (species)

SI-ID 40988

ATCC 35310, NCDO 2354, DSM 20616, CCUG 15557, NCFB 2354, JCM 12246, NCTC 13064

Section

Hoylesella buccalis HS4 is an anaerobe, mesophilic bacterium that was isolated from dental plaque.

anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacteroidota*
	Class	*Bacteroidia*
	Order	*Bacteroidales*
	Family	*Prevotellaceae*
	Genus	*Hoylesella*
	Species	**Hoylesella buccalis**
	Full Scientific Name (LPSN)	Hoylesella buccalis (Shah and Collins 1982) Hitch et al. 2023

	Synonym	Prevotella buccalis
	Synonym	Bacteroides buccalis

Information on morphological and physiological properties Morphology

[Ref.: #8964]

Incubation period

2-3 days

Information on culture and growth conditions Culture and growth conditions

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Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

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Culture medium

FASTIDIOUS ANAEROBE BROTH (DSMZ Medium 1203a)

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Culture medium growth

[Ref.: #8964]

Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

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Culture medium

PYG MEDIUM (MODIFIED) (DSMZ Medium 104)

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Culture medium growth

[Ref.: #8964]

Culture medium link

Medium recipe at MediaDive

[Ref.: #8964]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #8964]	growth	37 °C
[Ref.: #46278]	growth	37 °C
[Ref.: #67770]	growth	37 °C

[Ref.: #8964]	Temperature range	mesophilic
[Ref.: #46278]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #8964]	Oxygen tolerance	anaerobe
[Ref.: #46278]	Oxygen tolerance	anaerobe

[Ref.: #67770]

Observation

quinones: MK-11, MK-12

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68380]	arginine ChEBI	-	hydrolysis	* from API rID32A
[Ref.: #68380]	D-mannose ChEBI	-	fermentation	* from API rID32A
[Ref.: #68380]	L-glutamate ChEBI	-	degradation	* from API rID32A
[Ref.: #68380]	nitrate ChEBI	-	reduction	* from API rID32A
[Ref.: #68380]	raffinose ChEBI	-	fermentation	* from API rID32A
[Ref.: #68380]	tryptophan ChEBI	-	energy source	* from API rID32A
[Ref.: #68380]	urea ChEBI	-	hydrolysis	* from API rID32A

	Metabolite production	Metabolite	Production
[Ref.: #68380]		indole ChEBI	no	* from API rID32A

	Physiological tests	Metabolite	Indole test
[Ref.: #68380]		indole ChEBI	-	* from API rID32A

	Enzyme	Enzyme activity	EC number
[Ref.: #68380]	alanine arylamidase	+	3.4.11.2	* from API rID32A
[Ref.: #68380]	alkaline phosphatase	+	3.1.3.1	* from API rID32A
[Ref.: #68380]	alpha-arabinosidase	-	3.2.1.55	* from API rID32A
[Ref.: #68380]	alpha-galactosidase	+	3.2.1.22	* from API rID32A
[Ref.: #68380]	alpha-glucosidase	+	3.2.1.20	* from API rID32A
[Ref.: #68380]	arginine dihydrolase	-	3.5.3.6	* from API rID32A
[Ref.: #68380]	beta-galactosidase	+	3.2.1.23	* from API rID32A
[Ref.: #68380]	beta-glucuronidase	-	3.2.1.31	* from API rID32A
[Ref.: #68380]	glutamate decarboxylase	-	4.1.1.15	* from API rID32A
[Ref.: #68380]	glycin arylamidase	-		* from API rID32A
[Ref.: #68380]	leucine arylamidase	-	3.4.11.1	* from API rID32A
[Ref.: #68380]	leucyl glycin arylamidase	+	3.4.11.1	* from API rID32A
[Ref.: #68380]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API rID32A
[Ref.: #68380]	phenylalanine arylamidase	-		* from API rID32A
[Ref.: #68380]	proline-arylamidase	-	3.4.11.5	* from API rID32A
[Ref.: #68380]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API rID32A
[Ref.: #68380]	serine arylamidase	-		* from API rID32A
[Ref.: #68380]	tryptophan deaminase	-	4.1.99.1	* from API rID32A
[Ref.: #68380]	tyrosine arylamidase	-		* from API rID32A
[Ref.: #68380]	urease	-	3.5.1.5	* from API rID32A
	Only first 10 entries are displayed. Click here to see all.

API® rID32A:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Urease/urea hydrolysisURE	Arginine dihydrolaseADH (Arg)	alpha-Galactosidasealpha GAL	beta-Galactosidasebeta GAL	beta-Galactosidase 6-phosphatebeta GP	alpha-Glucosidasealpha GLU	beta-Glucosidasebeta GLU	alpha-Arabinosidasealpha ARA	beta-Glucuronidasebeta GUR	beta-N-Acetyl-beta-glucosaminidasebeta NAG	Fermentation of D-mannoseMNE	Fermentation of raffinoseRAF	Glutamate decarboxylaseGDC	alpha-Fucosidasealpha FUC	Reduction of nitrateNIT	Indole productionIND	Alkaline phosphatasePAL	L-arginine arylamidaseArgA	Proline arylamidaseProA	Leucyl glycin arylamidaseLGA	Phenylalanine arylamidasePheA	Leucine arylamidaseLeuA	Pyrrolidonyl arylamidasePyrA	Tyrosine arylamidaseTyrA	Alanine arylamidaseAlaA	Glycin arylamidaseGlyA	Histidine arylamidaseHisA	Glutamyl-glutamate arylamidaseGGA	Serine arylamidaseSerA
[Ref.: #8964]	13158	-	-	+	+	+/-	+	+	-	-	+	-	-	-	+	-	-	+	+	-	+	-	-	-	-	+	-	+/-	+/-	-
[Ref.: #8964]	12909	-	-	+	+	-	+	+/-	-	-	+	-	-	-	+/-	-	-	+	+	-	+	-	-	-	-	+	-	-	-	-
[Ref.: #8964]	12339	-	-	+	+	+/-	+	+	-	-	+	-	-	-	+	-	-	+	-	-	+	-	-	-	-	+	-	-	+	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #8964]	Sample type/isolated from	dental plaque

[Ref.: #46278]	Sample type/isolated from	Human dental plaque

[Ref.: #67770]	Sample type/isolated from	Dental plaque
* marker position based on {}

Isolation sources categories

#Host Body-Site

#Oral cavity and airways

#Plaque

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AB547676 (>99% sequence identity) for Prevotella buccalis subclade from Microbeatlas

Aquatic Samples	675
Soil Samples	904
Animal Samples	63648
Plant Samples	175
Total Samples	65402

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #8964]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #8964]	Prevotella buccalis ATCC 35310 16S ribosomal RNA gene, partial sequence	L16476	1474	ENA	679190 tax ID
[Ref.: #20218]	Prevotella buccalis gene for 16S ribosomal RNA, partial sequence, strain: JCM 12246	AB547676	1493	ENA	28127 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Hoylesella buccalis ATCC 35310	GCA_000177075	contig	GenBank	679190 tax ID
[Ref.: #66792]	Prevotella buccalis ATCC 35310	679190.3	wgs	PATRIC	679190 tax ID	*
[Ref.: #66792]	Prevotella buccalis ATCC 35310	647000293	draft	JGI IMG/M	679190 tax ID	*
[Ref.: #66792]	Hoylesella buccalis ATCC 35310	GCA_025151385	complete	GenBank	679190 tax ID	*
[Ref.: #66792]	Prevotella buccalis ATCC 35310	679190.26	complete	PATRIC	679190 tax ID	*

[Ref.: #8964]	GC-content	46.0 mol%
[Ref.: #67770]	GC-content	51 mol%	thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Prevotella buccalis ATCC 35310 ATCC 35310 NCBI: GCA_025151385.1
[Ref.: #69480]	motile	no	92.937	no
[Ref.: #69480]	flagellated	no	97.464	no
[Ref.: #69480]	gram-positive	no	96.911	no
[Ref.: #69480]	anaerobic	yes	98.175	no
[Ref.: #69480]	aerobic	no	97.639	yes
[Ref.: #69480]	halophile	no	82.986	no
[Ref.: #69480]	spore-forming	no	97.255	no
[Ref.: #69480]	glucose-ferment	yes	56.342	no
[Ref.: #69480]	thermophile	no	97.265	yes
[Ref.: #69480]	glucose-util	yes	86.941	no

Availability in culture collections External links

[Ref.: #8964]

Culture collection no.

CCUG 15557, NCTC 13064, JCM 12246, DSM 20616, ATCC 35310, NCDO 2354

[Ref.: #81747]

SI-ID 40988

References

#8964

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 20616

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#46278 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 15557

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68380

Automatically annotated from API rID32A .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#81747

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID40988.1 )

* These data were automatically processed and therefore are not curated

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Prevotella buccalis ATCC 35310 ATCC 35310

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