Name: Barnesiella intestinihominis DSM 21032
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 12519

Type strain:

Species: Barnesiella intestinihominis

Culture col. no.: DSM 21032, JCM 15079, YIT 11860

Strain history: M. Morotomi 7-38 (=YIT 11860).

NCBI tax ID(s): 742726 (strain), 487174 (species)

SI-ID 400531

DSM 21032

Section

Barnesiella intestinihominis DSM 21032 is an anaerobe, mesophilic, Gram-negative bacterium that was isolated from human faeces.

Gram-negative
rod-shaped
anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacteroidota*
	Class	*Bacteroidia*
	Order	*Bacteroidales*
	Family	*Barnesiellaceae*
	Genus	*Barnesiella*
	Species	**Barnesiella intestinihominis**
	Full Scientific Name (LPSN)	Barnesiella intestinihominis Morotomi et al. 2008

Information on morphological and physiological properties Morphology

[Ref.: #31196]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.932

[Ref.: #31196]	Cell length	1.3-3 µm

[Ref.: #31196]	Cell width	0.4-1 µm

[Ref.: #31196]	Cell shape	rod-shaped

[Ref.: #31196]	Motility	no
[Ref.: #69480]	Motility	no Confidence in %93.04

[Ref.: #15518]

Incubation period

2-3 days

[Ref.: #31196]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #15518]

Culture medium

CHOPPED MEAT MEDIUM (DSMZ Medium 78)

[Ref.: #15518]

Culture medium growth

[Ref.: #15518]

Culture medium link

Medium recipe at MediaDive

[Ref.: #15518]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #15518]	growth	37 °C
[Ref.: #31196]	growth	37 °C
[Ref.: #31196]	optimum	37 °C
[Ref.: #67770]	growth	37 °C

[Ref.: #15518]	Temperature range	mesophilic
[Ref.: #31196]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #31196]			optimum	7
[Ref.: #31196]			growth	7

Information on physiology and metabolism Physiology and metabolism

[Ref.: #15518]	Oxygen tolerance	anaerobe
[Ref.: #31196]	Oxygen tolerance	anaerobe
[Ref.: #69480]	Oxygen tolerance	anaerobe Confidence in %99.38

[Ref.: #31196]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.999

[Ref.: #31196]

Observation

aggregates in chains

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68380]	arginine ChEBI	-	hydrolysis	* from API rID32A
[Ref.: #68380]	D-mannose ChEBI	-	fermentation	* from API rID32A
[Ref.: #31196]	glucose ChEBI	+	carbon source
[Ref.: #68380]	L-glutamate ChEBI	+	degradation	* from API rID32A
[Ref.: #31196]	lactose ChEBI	+	carbon source
[Ref.: #31196]	maltose ChEBI	+	carbon source
[Ref.: #31196]	mannose ChEBI	+	carbon source
[Ref.: #68380]	nitrate ChEBI	-	reduction	* from API rID32A
[Ref.: #68380]	tryptophan ChEBI	-	energy source	* from API rID32A
[Ref.: #68380]	urea ChEBI	-	hydrolysis	* from API rID32A

	Metabolite production	Metabolite	Production
[Ref.: #68380]		indole ChEBI	no	* from API rID32A

	Physiological tests	Metabolite	Indole test
[Ref.: #68380]		indole ChEBI	-	* from API rID32A

	Enzyme	Enzyme activity	EC number
[Ref.: #31196]	acid phosphatase	+	3.1.3.2
[Ref.: #68380]	alanine arylamidase	+	3.4.11.2	* from API rID32A
[Ref.: #31196]	alkaline phosphatase	+	3.1.3.1
[Ref.: #68380]	alkaline phosphatase	+	3.1.3.1	* from API rID32A
[Ref.: #68380]	alpha-arabinosidase	-	3.2.1.55	* from API rID32A
[Ref.: #31196]	alpha-galactosidase	+	3.2.1.22
[Ref.: #68380]	alpha-glucosidase	+	3.2.1.20	* from API rID32A
[Ref.: #68380]	arginine dihydrolase	-	3.5.3.6	* from API rID32A
[Ref.: #68380]	beta-galactosidase	+	3.2.1.23	* from API rID32A
[Ref.: #68380]	beta-Galactosidase 6-phosphate	-		* from API rID32A
[Ref.: #68380]	beta-glucuronidase	-	3.2.1.31	* from API rID32A
[Ref.: #68380]	glutamate decarboxylase	+	4.1.1.15	* from API rID32A
[Ref.: #68380]	glutamyl-glutamate arylamidase	-		* from API rID32A
[Ref.: #68380]	histidine arylamidase	-		* from API rID32A
[Ref.: #68380]	L-arginine arylamidase	-		* from API rID32A
[Ref.: #68380]	leucine arylamidase	-	3.4.11.1	* from API rID32A
[Ref.: #68380]	leucyl glycin arylamidase	+	3.4.11.1	* from API rID32A
[Ref.: #68380]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API rID32A
[Ref.: #68380]	proline-arylamidase	-	3.4.11.5	* from API rID32A
[Ref.: #68380]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API rID32A
[Ref.: #68380]	serine arylamidase	-		* from API rID32A
[Ref.: #68380]	tryptophan deaminase	-	4.1.99.1	* from API rID32A
[Ref.: #68380]	urease	-	3.5.1.5	* from API rID32A
	Only first 10 entries are displayed. Click here to see all.

API® rID32A:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Urease/urea hydrolysisURE	Arginine dihydrolaseADH (Arg)	alpha-Galactosidasealpha GAL	beta-Galactosidasebeta GAL	beta-Galactosidase 6-phosphatebeta GP	alpha-Glucosidasealpha GLU	beta-Glucosidasebeta GLU	alpha-Arabinosidasealpha ARA	beta-Glucuronidasebeta GUR	beta-N-Acetyl-beta-glucosaminidasebeta NAG	Fermentation of D-mannoseMNE	Fermentation of raffinoseRAF	Glutamate decarboxylaseGDC	alpha-Fucosidasealpha FUC	Reduction of nitrateNIT	Indole productionIND	Alkaline phosphatasePAL	L-arginine arylamidaseArgA	Proline arylamidaseProA	Leucyl glycin arylamidaseLGA	Phenylalanine arylamidasePheA	Leucine arylamidaseLeuA	Pyrrolidonyl arylamidasePyrA	Tyrosine arylamidaseTyrA	Alanine arylamidaseAlaA	Glycin arylamidaseGlyA	Histidine arylamidaseHisA	Glutamyl-glutamate arylamidaseGGA	Serine arylamidaseSerA
[Ref.: #15518]	12986	-	-	+	+	-	+	+/-	-	-	+	-	-	+	+	-	-	+	-	-	+	+/-	-	-	+/-	+	+/-	-	-	-
[Ref.: #15518]	12385	-	-	+/-	+	-	+	+/-	-	-	+	-	+/-	+	+/-	-	-	+	-	-	+	-	-	-	-	+	-	-	-	-
[Ref.: #15518]	12384	-	-	+	+	-	+	+	-	-	+	-	-	+	+/-	-	-	+	-	-	+	-	-	-	-	+	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #15518]	Sample type/isolated from	human faeces
[Ref.: #15518]	Geographic location (country and/or sea, region)	Tokyo
[Ref.: #15518]	Country	Japan
[Ref.: #15518]	Country ISO 3 Code	JPN
[Ref.: #15518]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Human feces
* marker position based on {}

Isolation sources categories

#Host	#Human	-
#Host Body Product	#Gastrointestinal tract	#Feces (Stool)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AB547647 (>99% sequence identity) for Barnesiella intestinihominis subclade from Microbeatlas

Aquatic Samples	1533
Soil Samples	278
Animal Samples	64067
Plant Samples	103
Total Samples	65981

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #15518]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #15518]	Barnesiella intestinihominis gene for 16S ribosomal RNA, partial sequence	AB370251	1484	ENA	742726 tax ID
[Ref.: #20218]	Barnesiella intestinihominis gene for 16S ribosomal RNA, partial sequence, strain: JCM 15079	AB547647	1481	ENA	487174 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Barnesiella intestinihominis YIT 11860	GCA_000296465	scaffold	GenBank	742726 tax ID
[Ref.: #66792]	Barnesiella intestinihominis YIT 11860	742726.3	wgs	PATRIC	742726 tax ID	*
[Ref.: #66792]	Barnesiella intestinihominis YIT 11860	2529292932	draft	JGI IMG/M	742726 tax ID	*

[Ref.: #15518]	GC-content	45.5 mol%
[Ref.: #15518]	GC-content	43.9 mol%	sequence analysis
[Ref.: #67770]	GC-content	45.5 mol%	high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Barnesiella intestinihominis YIT 11860 YIT 11860 NCBI: GCA_000296465.1
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Barnesiella intestinihominis YIT 11860 PATRIC: 742726.3
[Ref.: #69480]	motile	no	91.647	yes
[Ref.: #69480]	flagellated	no	96.489	yes
[Ref.: #69480]	gram-positive	no	97.794	yes
[Ref.: #69480]	anaerobic	yes	99.211	yes
[Ref.: #69480]	aerobic	no	98.478	yes
[Ref.: #69480]	halophile	no	85.896	no
[Ref.: #69480]	spore-forming	no	95.639	yes
[Ref.: #69480]	glucose-util	yes	85.595	yes
[Ref.: #69480]	thermophile	no	97.959	yes
[Ref.: #69480]	glucose-ferment	yes	58.534	no

Availability in culture collections External links

[Ref.: #15518]

Culture collection no.

DSM 21032, JCM 15079, YIT 11860

[Ref.: #81738]

SI-ID 400531

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Dialister succinatiphilus sp. nov. and Barnesiella intestinihominis sp. nov., isolated from human faeces.	Morotomi M, Nagai F, Sakon H, Tanaka R	Int J Syst Evol Microbiol	10.1099/ijs.0.2008/000810-0	2008	*

References

#15518

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 21032

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#31196

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27523 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68380

Automatically annotated from API rID32A .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#81738

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID400531.1 )

#27523

IJSEM 2716 2008 ( DOI 10.1099/ijs.0.2008/000810-0 , PubMed 19060046 )

* These data were automatically processed and therefore are not curated

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Barnesiella intestinihominis YIT 11860 YIT 11860

Barnesiella intestinihominis YIT 11860

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