Proteiniphilum acetatigenes TB107 | Type strain | DSM 18083, CGMCC 1.5024, JCM 12891, CCUG 53886 | BacDiveID:12514

Strain identifier

BacDive ID: 12514

Type strain:

Species: Proteiniphilum acetatigenes

Strain Designation: TB107

Culture col. no.: DSM 18083, CGMCC 1.5024, JCM 12891, CCUG 53886

Strain history: S. Chen and X. Dong TB107.

NCBI tax ID(s): 1123008 (strain), 294710 (species)

SI-ID 281464

AS 1.5024, JCM 12891, DSM 18083, CGMCC 1.5024, CCUG 53886

Section

Proteiniphilum acetatigenes TB107 is an anaerobe, mesophilic, Gram-negative bacterium that was isolated from granule sludge, UASB reactor treating brewery wastewater.

Gram-negative
motile
rod-shaped
anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacteroidota*
	Class	*Bacteroidia*
	Order	*Bacteroidales*
	Family	*Dysgonomonadaceae*
	Genus	*Proteiniphilum*
	Species	**Proteiniphilum acetatigenes**
	Full Scientific Name (LPSN)	Proteiniphilum acetatigenes Chen and Dong 2005

Information on morphological and physiological properties Morphology

[Ref.: #31558]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.992

[Ref.: #31558]	Cell length	2.05 µm

[Ref.: #31558]	Cell width	0.75 µm

[Ref.: #31558]	Cell shape	rod-shaped

[Ref.: #31558]	Motility	yes

Incubation period

1-2 days

Multimedia content

Caption:

electron microscopic image

Intellectual property rights:

© HZI/Manfred Rohde

Caption:

electron microscopic image

Intellectual property rights:

© HZI/Manfred Rohde

Caption:

electron microscopic image

Intellectual property rights:

© HZI/Manfred Rohde

Caption:

electron microscopic image

Intellectual property rights:

© HZI/Manfred Rohde

Caption:

electron microscopic image

Intellectual property rights:

© HZI/Manfred Rohde

Information on culture and growth conditions Culture and growth conditions

Culture medium

PYG MEDIUM (MODIFIED) (DSMZ Medium 104)

Culture medium growth

Culture medium link

Medium recipe at MediaDive

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #7384]			growth	37 °C
[Ref.: #31558]			growth	20-45 °C
[Ref.: #31558]			optimum	37 °C
[Ref.: #67770]			growth	37 °C

[Ref.: #7384]	Temperature range	mesophilic
[Ref.: #31558]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #31558]			optimum	7.75
[Ref.: #31558]			growth	6-9.7

Information on physiology and metabolism Physiology and metabolism

[Ref.: #7384]	Oxygen tolerance	anaerobe
[Ref.: #31558]	Oxygen tolerance	anaerobe
[Ref.: #69480]	Oxygen tolerance	anaerobe Confidence in %97.961

[Ref.: #31558]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.981

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #31558]		NaCl		growth	0-5	%
[Ref.: #31558]		NaCl		optimum	2.5	%

	Metabolite utilization	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #31558]		acetate ChEBI	+	carbon source
[Ref.: #31558]		alanine ChEBI	+	carbon source
[Ref.: #31558]		arginine ChEBI	+	carbon source
[Ref.: #68380]		arginine ChEBI	-	hydrolysis	* from API rID32A
[Ref.: #31558]		esculin ChEBI	+	hydrolysis
[Ref.: #31558]		glycine ChEBI	+	carbon source
[Ref.: #68380]		L-glutamate ChEBI	-	degradation	* from API rID32A
[Ref.: #68380]		nitrate ChEBI	-	reduction	* from API rID32A
[Ref.: #31558]		propionate ChEBI	+	carbon source
[Ref.: #31558]		pyruvate ChEBI	+	carbon source
[Ref.: #31558]		serine ChEBI	+	carbon source
[Ref.: #31558]		threonine ChEBI	+	carbon source
[Ref.: #68380]		tryptophan ChEBI	-	energy source	* from API rID32A
[Ref.: #68380]		urea ChEBI	-	hydrolysis	* from API rID32A
		Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #68380]		indole ChEBI	no	* from API rID32A

	Physiological tests	Metabolite	Indole test
[Ref.: #68380]		indole ChEBI	-	* from API rID32A

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #68380]		alanine arylamidase	+	3.4.11.2	* from API rID32A
[Ref.: #68380]		alkaline phosphatase	+	3.1.3.1	* from API rID32A
[Ref.: #68380]		alpha-fucosidase	-	3.2.1.51	* from API rID32A
[Ref.: #68380]		alpha-galactosidase	+	3.2.1.22	* from API rID32A
[Ref.: #68380]		alpha-glucosidase	+	3.2.1.20	* from API rID32A
[Ref.: #68380]		arginine dihydrolase	-	3.5.3.6	* from API rID32A
[Ref.: #68380]		beta-galactosidase	+	3.2.1.23	* from API rID32A
[Ref.: #68380]		beta-Galactosidase 6-phosphate	-		* from API rID32A
[Ref.: #68380]		beta-glucosidase	+	3.2.1.21	* from API rID32A
[Ref.: #68380]		beta-glucuronidase	-	3.2.1.31	* from API rID32A
[Ref.: #68380]		glutamate decarboxylase	-	4.1.1.15	* from API rID32A
[Ref.: #68380]		glycin arylamidase	-		* from API rID32A
[Ref.: #68380]		histidine arylamidase	-		* from API rID32A
[Ref.: #68380]		L-arginine arylamidase	-		* from API rID32A
[Ref.: #68380]		leucine arylamidase	-	3.4.11.1	* from API rID32A
[Ref.: #68380]		leucyl glycin arylamidase	+	3.4.11.1	* from API rID32A
[Ref.: #68380]		N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API rID32A
[Ref.: #68380]		phenylalanine arylamidase	-		* from API rID32A
[Ref.: #68380]		proline-arylamidase	-	3.4.11.5	* from API rID32A
[Ref.: #68380]		pyrrolidonyl arylamidase	-	3.4.19.3	* from API rID32A
[Ref.: #68380]		serine arylamidase	-		* from API rID32A
[Ref.: #68380]		tryptophan deaminase	-	4.1.99.1	* from API rID32A
[Ref.: #68380]		tyrosine arylamidase	-		* from API rID32A
[Ref.: #68380]		urease	-	3.5.1.5	* from API rID32A
		Only first 10 entries are displayed. Click here to see all.

API® rID32A:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Urease/urea hydrolysisURE	Arginine dihydrolaseADH (Arg)	alpha-Galactosidasealpha GAL	beta-Galactosidasebeta GAL	beta-Galactosidase 6-phosphatebeta GP	alpha-Glucosidasealpha GLU	beta-Glucosidasebeta GLU	alpha-Arabinosidasealpha ARA	beta-Glucuronidasebeta GUR	beta-N-Acetyl-beta-glucosaminidasebeta NAG	Fermentation of D-mannoseMNE	Fermentation of raffinoseRAF	Glutamate decarboxylaseGDC	alpha-Fucosidasealpha FUC	Reduction of nitrateNIT	Indole productionIND	Alkaline phosphatasePAL	L-arginine arylamidaseArgA	Proline arylamidaseProA	Leucyl glycin arylamidaseLGA	Phenylalanine arylamidasePheA	Leucine arylamidaseLeuA	Pyrrolidonyl arylamidasePyrA	Tyrosine arylamidaseTyrA	Alanine arylamidaseAlaA	Glycin arylamidaseGlyA	Histidine arylamidaseHisA	Glutamyl-glutamate arylamidaseGGA	Serine arylamidaseSerA
[Ref.: #7384]	12226	-	-	+	+	-	+	+	+	-	+	+	+	-	-	-	-	+	-	-	+	-	-	-	-	+	-	-	+/-	-
[Ref.: #7384]	12225	-	-	+	+	-	+	+	-	-	+	+/-	+/-	-	-	-	-	+	-	-	+	-	-	-	-	+	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #7384]	Sample type/isolated from	granule sludge, UASB reactor treating brewery wastewater
[Ref.: #7384]	Country	China
[Ref.: #7384]	Country ISO 3 Code	CHN
[Ref.: #7384]	Continent	Asia

[Ref.: #60055]	Sample type/isolated from	Granule sludge,UASB reactor,brewery waistwater

[Ref.: #67770]	Sample type/isolated from	Granule sludge of a UASB reactor treating brewery wastewater
* marker position based on {}

Isolation sources categories

#Engineered	#Biodegradation	#Anaerobic digestor
#Engineered	#Waste	#Industrial wastewater
#Engineered	#Waste	#Activated sludge

What are isolation sources categories?

Global distribution of 16S sequence AY742226 (>99% sequence identity) for Proteiniphilum acetatigenes subclade from Microbeatlas

Aquatic Samples	150
Soil Samples	86
Animal Samples	1236
Plant Samples	86
Total Samples	1558

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

Biosafety level

1

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #7384]	Proteiniphilum acetatigenes strain TB107 16S ribosomal RNA gene, complete sequence	AY742226	1523	ENA	294710 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Proteiniphilum acetatigenes DSM 18083	GCA_000380985	scaffold	GenBank	1123008 tax ID
[Ref.: #66792]	Proteiniphilum acetatigenes DSM 18083	1123008.3	wgs	PATRIC	1123008 tax ID	*
[Ref.: #66792]	Proteiniphilum acetatigenes DSM 18083	2518645612	draft	JGI IMG/M	1123008 tax ID	*

[Ref.: #7384]	GC-content	46.6 mol%
[Ref.: #7384]	GC-content	43.1 mol%	sequence analysis
[Ref.: #67770]	GC-content	38 mol%	thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Proteiniphilum acetatigenes DSM 18083 DSM 18083 NCBI: GCA_000380985.1
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Proteiniphilum acetatigenes DSM 18083 PATRIC: 1123008.3
[Ref.: #69480]	motile	no	80.007	yes
[Ref.: #69480]	flagellated	no	97.689	no
[Ref.: #69480]	gram-positive	no	95.201	yes
[Ref.: #69480]	anaerobic	yes	61.243	no
[Ref.: #69480]	aerobic	no	90.773	yes
[Ref.: #69480]	halophile	no	83.225	no
[Ref.: #69480]	spore-forming	no	91.772	yes
[Ref.: #69480]	glucose-util	yes	88.467	no
[Ref.: #69480]	thermophile	no	99.137	no
[Ref.: #69480]	glucose-ferment	yes	57.928	no

Availability in culture collections External links

Culture collection no.

DSM 18083, CGMCC 1.5024, JCM 12891, CCUG 53886

SI-ID 281464

*

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Proteiniphilum acetatigenes gen. nov., sp. nov., from a UASB reactor treating brewery wastewater.	Chen S, Dong X	Int J Syst Evol Microbiol	10.1099/ijs.0.63807-0	2005	*

References

#7384

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 18083

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#31558

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27848 (see below)

#60055 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 53886

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#66793

Mukherjee et al.: GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life. 35: 676 - 683 2017 ( DOI 10.1038/nbt.3886 , PubMed 28604660 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68380

Automatically annotated from API rID32A .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#81733

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID281464.1 )

#27848

IJSEM 2257 2005 ( DOI 10.1099/ijs.0.63807-0 , PubMed 16280479 )

* These data were automatically processed and therefore are not curated

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