Name: Porphyromonas levii LEV
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 12508

Type strain:

Species: Porphyromonas levii

Strain Designation: LEV

Culture col. no.: DSM 23370, ATCC 29147, CCUG 34320, JCM 13866, NCTC 11028, ACM 5042, CCUG 21027, HAMBI 467, VPI 10450, VPI 3300

Strain history: CCUG 34320 <-- NCTC 11028 <-- M. Lev strain LEV 1.

NCBI tax ID(s): 1122973 (strain), 28114 (species)

SI-ID 40353

ATCC 29147, CCUG 21027, CCUG 34320, NCTC 11028, HAMBI 467, ACM 5042, JCM 13866, DSM 23370

Section

Porphyromonas levii LEV is an anaerobe, mesophilic, Gram-negative human pathogen that was isolated from bovine rumen.

Gram-negative
anaerobe
human pathogen
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacteroidota*
	Class	*Bacteroidia*
	Order	*Bacteroidales*
	Family	*Porphyromonadaceae*
	Genus	*Porphyromonas*
	Species	**Porphyromonas levii**
	Full Scientific Name (LPSN)	Porphyromonas levii (Johnson and Holdeman 1983 ex Holdeman et al. 1977) Shah et al. 1995

Synonym

Bacteroides levii

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	negative Confidence in %99.998

[Ref.: #16711]	Type of hemolysis	gamma
[Ref.: #16711]	Incubation period	1-2 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #16711]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #16711]

Culture medium growth

[Ref.: #16711]

Culture medium link

Medium recipe at MediaDive

[Ref.: #16711]

Culture medium composition

expand / minimize

[Ref.: #16711]

Culture medium

CHOPPED MEAT MEDIUM (DSMZ Medium 78)

[Ref.: #16711]

Culture medium growth

[Ref.: #16711]

Culture medium link

Medium recipe at MediaDive

[Ref.: #16711]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #16711]			growth	37 °C
[Ref.: #67770]			growth	37 °C

[Ref.: #16711]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #16711]	Oxygen tolerance	anaerobe
[Ref.: #69480]	Oxygen tolerance	anaerobe Confidence in %99.622

[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.999

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68380]	arginine ChEBI	-	hydrolysis	* from API rID32A
[Ref.: #68367]	cellobiose ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	D-glucose ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	D-mannitol ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	D-mannose ChEBI	-	builds acid from	* from API 20A
[Ref.: #68380]	D-mannose ChEBI	-	fermentation	* from API rID32A
[Ref.: #68367]	D-xylose ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	esculin ChEBI	-	hydrolysis	* from API 20A
[Ref.: #68367]	gelatin ChEBI	+	hydrolysis	* from API 20A
[Ref.: #68367]	glycerol ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	L-arabinose ChEBI	-	builds acid from	* from API 20A
[Ref.: #68380]	L-glutamate ChEBI	-	degradation	* from API rID32A
[Ref.: #68367]	L-rhamnose ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	lactose ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	maltose ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	melezitose ChEBI	-	builds acid from	* from API 20A
[Ref.: #68380]	nitrate ChEBI	-	reduction	* from API rID32A
[Ref.: #68367]	raffinose ChEBI	-	builds acid from	* from API 20A
[Ref.: #68380]	raffinose ChEBI	-	fermentation	* from API rID32A
[Ref.: #68367]	salicin ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	sorbitol ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	sucrose ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	trehalose ChEBI	-	builds acid from	* from API 20A
[Ref.: #68380]	tryptophan ChEBI	-	energy source	* from API rID32A
[Ref.: #68367]	urea ChEBI	-	hydrolysis	* from API 20A, API rID32A
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #68380]		indole ChEBI	no	* from API rID32A

	Physiological tests	Metabolite	Indole test
[Ref.: #68380]		indole ChEBI	-	* from API rID32A

	Enzyme	Enzyme activity	EC number
[Ref.: #68380]	alanine arylamidase	+	3.4.11.2	* from API rID32A
[Ref.: #68380]	alkaline phosphatase	+	3.1.3.1	* from API rID32A
[Ref.: #68380]	alpha-arabinosidase	-	3.2.1.55	* from API rID32A
[Ref.: #68380]	alpha-fucosidase	-	3.2.1.51	* from API rID32A
[Ref.: #68380]	alpha-galactosidase	-	3.2.1.22	* from API rID32A
[Ref.: #68380]	alpha-glucosidase	-	3.2.1.20	* from API rID32A
[Ref.: #68380]	arginine dihydrolase	-	3.5.3.6	* from API rID32A
[Ref.: #68380]	beta-galactosidase	+	3.2.1.23	* from API rID32A
[Ref.: #68380]	beta-Galactosidase 6-phosphate	-		* from API rID32A
[Ref.: #68380]	beta-glucosidase	-	3.2.1.21	* from API rID32A, API 20A
[Ref.: #68380]	beta-glucuronidase	-	3.2.1.31	* from API rID32A
[Ref.: #16711]	catalase	-	1.11.1.6
[Ref.: #16711]	cytochrome-c oxidase	-	1.9.3.1
[Ref.: #68367]	gelatinase	+		* from API 20A
[Ref.: #68380]	glutamate decarboxylase	-	4.1.1.15	* from API rID32A
[Ref.: #68380]	glutamyl-glutamate arylamidase	+		* from API rID32A
[Ref.: #68380]	histidine arylamidase	-		* from API rID32A
[Ref.: #68380]	L-arginine arylamidase	-		* from API rID32A
[Ref.: #68380]	leucine arylamidase	-	3.4.11.1	* from API rID32A
[Ref.: #68380]	leucyl glycin arylamidase	+	3.4.11.1	* from API rID32A
[Ref.: #68380]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API rID32A
[Ref.: #68380]	serine arylamidase	-		* from API rID32A
[Ref.: #68380]	tryptophan deaminase	-	4.1.99.1	* from API rID32A
[Ref.: #68380]	urease	-	3.5.1.5	* from API rID32A, API 20A
	Only first 10 entries are displayed. Click here to see all.

API® 20A:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Indole productionIND	Urease/urea hydrolysisURE	Acid from D-glucoseGLU	Acid from D-mannitolMAN	Acid from lactoseLAC	Acid from sucroseSAC	Acid from maltoseMAL	Acid from salicinSAL	Acid from D-xyloseXYL	Acid from L-arabinoseARA	Gelatinase/gelatin hydrolysisGEL	Esculin hydrolysis/beta-glucosidaseESC	Acid from glycerolGLY	Acid from cellobioseCEL	Acid from D-mannoseMNE	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from sorbitolSOR	Acid from L-rhamnoseRHA	Acid from trehaloseTRE	CatalaseCAT	Spores presentSPOR	GramGRAM	Morphology coccus="+" rod="-"COCC
[Ref.: #16711]	2080	not determinedn.d.	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API® rID32A:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Urease/urea hydrolysisURE	Arginine dihydrolaseADH (Arg)	alpha-Galactosidasealpha GAL	beta-Galactosidasebeta GAL	beta-Galactosidase 6-phosphatebeta GP	alpha-Glucosidasealpha GLU	beta-Glucosidasebeta GLU	alpha-Arabinosidasealpha ARA	beta-Glucuronidasebeta GUR	beta-N-Acetyl-beta-glucosaminidasebeta NAG	Fermentation of D-mannoseMNE	Fermentation of raffinoseRAF	Glutamate decarboxylaseGDC	alpha-Fucosidasealpha FUC	Reduction of nitrateNIT	Indole productionIND	Alkaline phosphatasePAL	L-arginine arylamidaseArgA	Proline arylamidaseProA	Leucyl glycin arylamidaseLGA	Phenylalanine arylamidasePheA	Leucine arylamidaseLeuA	Pyrrolidonyl arylamidasePyrA	Tyrosine arylamidaseTyrA	Alanine arylamidaseAlaA	Glycin arylamidaseGlyA	Histidine arylamidaseHisA	Glutamyl-glutamate arylamidaseGGA	Serine arylamidaseSerA
[Ref.: #16711]	13323	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	+	-	+/-	+	+/-	-	-	+/-	+	+/-	-	+	-
[Ref.: #16711]	12425	-	-	-	+	-	-	-	-	-	+	-	-	-	-	-	-	+	-	-	+	-	-	-	-	+	-	-	+	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #16711]	Sample type/isolated from	bovine rumen

[Ref.: #67770]	Sample type/isolated from	Bovine rumen
* marker position based on {}

Isolation sources categories

#Host	#Mammals	#Bovinae (Cow, Cattle)
#Host Body-Site	#Organ	#Rumen

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence FJ792536 (>99% sequence identity) for Porphyromonas levii subclade from Microbeatlas

Aquatic Samples	607
Soil Samples	894
Animal Samples	6931
Plant Samples	148
Total Samples	8580

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #16711]	Pathogenicity (human)	yes
[Ref.: #16711]	Pathogenicity (animal)	yes
[Ref.: #16711]	Biosafety level	2	Risk group (German classification) According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #16711]	Porphyromonas levii ATCC 29147 16S ribosomal RNA gene, complete sequence	L16493	1476	ENA	28114 tax ID
[Ref.: #20218]	Porphyromonas levii strain ATCC 29147 16S ribosomal RNA gene, partial sequence	FJ792536	1375	ENA	1122973 tax ID	*
[Ref.: #20218]	Porphyromonas levii strain ATCC 29147 16S-23S ribosomal RNA intergenic spacer, partial sequence	AY546480	852	ENA	1122973 tax ID	*
[Ref.: #20218]	Porphyromonas levii gene for 16S ribosomal RNA, partial sequence, strain: JCM 13866	AB547664	1495	ENA	1122973 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Porphyromonas levii DSM 23370	GCA_000379925	scaffold	GenBank	1122973 tax ID
[Ref.: #66792]	Porphyromonas levii DSM 23370	1122973.3	wgs	PATRIC	1122973 tax ID	*
[Ref.: #66792]	Porphyromonas levii DSM 23370	2515154110	draft	JGI IMG/M	1122973 tax ID	*

[Ref.: #16711]	GC-content	46 mol%
[Ref.: #16711]	GC-content	45.7 mol%	sequence analysis
[Ref.: #67770]	GC-content	45-46 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #67770]	GC-content	46-48 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Porphyromonas levii DSM 23370 PATRIC: 1122973.3
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Porphyromonas levii DSM 23370 PATRIC: 1122973.3
[Ref.: #69480]	motile	no	91.444	no
[Ref.: #69480]	flagellated	no	97.088	no
[Ref.: #69480]	gram-positive	no	96.581	no
[Ref.: #69480]	anaerobic	yes	99.052	no
[Ref.: #69480]	aerobic	no	97.577	no
[Ref.: #69480]	halophile	no	88.255	no
[Ref.: #69480]	spore-forming	no	96.593	no
[Ref.: #69480]	thermophile	no	97.745	no
[Ref.: #69480]	glucose-util	yes	78.963	no
[Ref.: #69480]	glucose-ferment	no	53.05	no

Availability in culture collections External links

[Ref.: #16711]

Culture collection no.

DSM 23370, ATCC 29147, CCUG 34320, JCM 13866, NCTC 11028, ACM 5042, CCUG 21027, HAMBI 467, VPI 10450, VPI 3300

[Ref.: #81727]

SI-ID 40353

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Porphyromonas somerae sp. nov., a pathogen isolated from humans and distinct from porphyromonas levii.	Summanen PH, Durmaz B, Vaisanen ML, Liu C, Molitoris D, Eerola E, Helander IM, Finegold SM	J Clin Microbiol	10.1128/JCM.43.9.4455-4459.2005	2005	*

References

#16711

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 23370

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68367

Automatically annotated from API 20A .

#68380

Automatically annotated from API rID32A .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#81727

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID40353.1 )

* These data were automatically processed and therefore are not curated

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Porphyromonas levii DSM 23370

Porphyromonas levii DSM 23370

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