Name: Fictibacillus macauensis ZFHKF-1
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 1245

Type strain:

Species: Fictibacillus macauensis

Strain Designation: ZFHKF-1

Culture col. no.: DSM 17262, JCM 13285, CIP 109160, NCIMB 14300

Strain history: CIP <- 2006, JCM, Bacillus macauensis <- 2005, H.H.P. Fang, Hong Kong Univ., Hong Kong, China: strain ZFHKF-1 <- DIT030428, Bacillus macauensis

NCBI tax ID(s): 245160 (species)

SI-ID 289967

DSM 17262, JCM 13285, CIP 109160

Section

Fictibacillus macauensis ZFHKF-1 is a facultative anaerobe, spore-forming, mesophilic bacterium that was isolated from drinking water supply.

Gram-positive
rod-shaped
spore-forming
facultative anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Fictibacillus*
	Species	**Fictibacillus macauensis**
	Full Scientific Name (LPSN)	Fictibacillus macauensis (Zhang et al. 2006) Glaeser et al. 2013

Synonym

Bacillus macauensis

Information on morphological and physiological properties Morphology

[Ref.: #31597]	Gram stain	positive
[Ref.: #120731]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #31597]	Cell length	02-05 µm

[Ref.: #31597]	Cell width	0.8-1.2 µm

[Ref.: #31597]	Cell shape	rod-shaped
[Ref.: #120731]	Cell shape	rod-shaped

[Ref.: #120731]	Motility	yes
[Ref.: #69480]	Motility	yes Confidence in %95.314

[Ref.: #120731]

Hemolysis ability

[Ref.: #31597]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #6862]

Culture medium

CASO AGAR (MERCK 105458) (DSMZ Medium 220)

[Ref.: #6862]

Culture medium growth

[Ref.: #6862]

Culture medium link

Medium recipe at MediaDive

[Ref.: #6862]

Culture medium composition

expand / minimize

[Ref.: #33279]

Culture medium

MEDIUM 368 - for Luria bertani

[Ref.: #33279]

Culture medium growth

[Ref.: #33279]

Culture medium composition

expand / minimize

[Ref.: #120731]

Culture medium

CIP Medium 368

[Ref.: #120731]

Culture medium growth

[Ref.: #120731]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #6862]	growth	30 °C
[Ref.: #31597]	growth	20-40 °C
[Ref.: #31597]	optimum	30 °C
[Ref.: #33279]	growth	30 °C
[Ref.: #67770]	growth	30 °C

[Ref.: #6862]	Temperature range	mesophilic
[Ref.: #31597]	Temperature range	mesophilic
[Ref.: #33279]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #31597]			optimum	8.5
[Ref.: #31597]			growth	06-10

Information on physiology and metabolism Physiology and metabolism

[Ref.: #31597]	Oxygen tolerance	facultative anaerobe
[Ref.: #120731]	Oxygen tolerance	obligate aerobe

[Ref.: #31597]	Ability of spore formation	yes
[Ref.: #69481]	Ability of spore formation	yes Confidence in %100
[Ref.: #69480]	Ability of spore formation	yes Confidence in %100

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #31597]		NaCl		growth	2	%
[Ref.: #31597]		NaCl		optimum	2	%

[Ref.: #31597]	Observation	aggregates in chains
[Ref.: #67770]	Observation	quinones: MK-7

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68371]	amygdalin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	arbutin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31597]	cellobiose ChEBI	+	carbon source
[Ref.: #68371]	cellobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fructose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-galactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	esculin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31597]	galactose ChEBI	+	carbon source
[Ref.: #68371]	gentiobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31597]	lactate ChEBI	+	carbon source
[Ref.: #68371]	lactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31597]	malate ChEBI	+	carbon source
[Ref.: #31597]	mannitol ChEBI	+	carbon source
[Ref.: #31597]	mannose ChEBI	+	carbon source
[Ref.: #68371]	melezitose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melibiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	N-acetylglucosamine ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #120731]	nitrate ChEBI	-	reduction
[Ref.: #120731]	nitrite ChEBI	-	reduction
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31597]	pyruvate ChEBI	+	carbon source
[Ref.: #68371]	raffinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31597]	rhamnose ChEBI	+	carbon source
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31597]	ribose ChEBI	+	carbon source
[Ref.: #31597]	salicin ChEBI	+	carbon source
[Ref.: #68371]	salicin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	starch ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31597]	sucrose ChEBI	+	carbon source
[Ref.: #68371]	sucrose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	trehalose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	turanose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #120731]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #120731]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	+	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #31597]	catalase	+	1.11.1.6
[Ref.: #120731]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #120731]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #120731]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #120731]	oxidase	+
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #120731]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #120731]	11489	not determinedn.d.	+/-	-	-	-	+/-	-	-	-	-	-	+/-	-	+/-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+/-	-	-	-	-	-	-	-	-	+/-	-	-	-	-	-	-	-	-	-	-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #120731]	11690	-	+	+	+	-	+	-	-	-	+	+	+	-	+	-	+	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #6862]	Sample type/isolated from	drinking water supply
[Ref.: #6862]	Geographic location (country and/or sea, region)	Macau
[Ref.: #6862]	Country	China
[Ref.: #6862]	Country ISO 3 Code	CHN
[Ref.: #6862]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Influent of a drinking water treatment plant in Macau
[Ref.: #67770]	Country	China
[Ref.: #67770]	Country ISO 3 Code	CHN
[Ref.: #67770]	Continent	Asia

[Ref.: #120731]	Sample type/isolated from	Environment, Drinking water, supply of Macao
[Ref.: #120731]	Geographic location (country and/or sea, region)	Pearl river
[Ref.: #120731]	Country	China
[Ref.: #120731]	Country ISO 3 Code	CHN
[Ref.: #120731]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental

#Aquatic

#Freshwater

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AY373018 (>99% sequence identity) for Fictibacillus macauensis subclade from Microbeatlas

Aquatic Samples	27
Soil Samples	158
Animal Samples	141
Plant Samples	10
Total Samples	336

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #6862]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #120731]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #6862]	Bacillus macauensis strain ZFHKF-1 16S ribosomal RNA gene, partial sequence	AY373018	1355	ENA	1196324 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Fictibacillus macauensis ZFHKF-1	GCA_000269865	contig	GenBank	1196324 tax ID
[Ref.: #66792]	Bacillus macauensis ZFHKF-1	1196324.3	wgs	PATRIC	1196324 tax ID	*
[Ref.: #66792]	Fictibacillus macauensis ZFHKF-1	2524023182	draft	JGI IMG/M	1196324 tax ID	*

[Ref.: #6862]	GC-content	40.8 mol%
[Ref.: #67770]	GC-content	40.6 mol%	genome sequence analysis
[Ref.: #67770]	GC-content	40.8 mol%	high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Bacillus macauensis ZFHKF-1 PATRIC: 1196324.3
[Ref.: #69481]	spore-forming	yes	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Bacillus macauensis ZFHKF-1 PATRIC: 1196324.3
[Ref.: #69480]	motile	yes	82.747	no
[Ref.: #69480]	gram-positive	yes	92.113	yes
[Ref.: #69480]	anaerobic	no	98.234	yes
[Ref.: #69480]	aerobic	yes	85.709	yes
[Ref.: #69480]	halophile	no	87.691	no
[Ref.: #69480]	spore-forming	yes	95.564	no
[Ref.: #69480]	glucose-util	yes	89.688	no
[Ref.: #69480]	flagellated	yes	65.995	no
[Ref.: #69480]	thermophile	no	92.758	yes
[Ref.: #69480]	glucose-ferment	no	91.227	no

Availability in culture collections External links

[Ref.: #6862]

Culture collection no.

DSM 17262, JCM 13285, CIP 109160, NCIMB 14300

[Ref.: #70901]

SI-ID 289967

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Fictibacillus phosphorivorans gen. nov., sp. nov. and proposal to reclassify Bacillus arsenicus, Bacillus barbaricus, Bacillus macauensis, Bacillus nanhaiensis, Bacillus rigui, Bacillus solisalsi and Bacillus gelatini in the genus Fictibacillus.	Glaeser SP, Dott W, Busse HJ, Kampfer P	Int J Syst Evol Microbiol	10.1099/ijs.0.049171-0	2013	*
Genetics	Genome of Bacillus macauensis ZFHKF-1, a long-chain-forming bacterium.	Cai L, Zhang T	J Bacteriol	10.1128/JB.01049-12	2012	*
Phylogeny	Bacillus solisalsi sp. nov., a halotolerant, alkaliphilic bacterium isolated from soil around a salt lake.	Liu H, Zhou Y, Liu R, Zhang KY, Lai R	Int J Syst Evol Microbiol	10.1099/ijs.0.000653-0	2009	*
Phylogeny	Bacillus tianmuensis sp. nov., isolated from soil in Tianmu Mountain national natural reserve, Hangzhou, China.	Wen YP, Wu XC, Qian CD, Zhao YH, Fang HH, Li O	FEMS Microbiol Lett	10.1111/j.1574-6968.2009.01610.x	2009	*
Phylogeny	Bacillus macauensis sp. nov., a long-chain bacterium isolated from a drinking water supply.	Zhang T, Fan X, Hanada S, Kamagata Y, Fang HHP	Int J Syst Evol Microbiol	10.1099/ijs.0.63868-0	2006	*

References

#6862

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 17262

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#31597

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27885 (see below)

#33279 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68371

Automatically annotated from API 50CH acid .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#70901

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID289967.1 )

#120731 Collection of Institut Pasteur ; Curators of the CIP; CIP 109160

#27885

IJSEM 349 2006 ( DOI 10.1099/ijs.0.63868-0 , PubMed 16449438 )

* These data were automatically processed and therefore are not curated

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Bacillus macauensis ZFHKF-1

Bacillus macauensis ZFHKF-1

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