Name: Bacillus carboniphilus DSM 17613
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 1239

Type strain:

Species: Bacillus carboniphilus

Culture col. no.: DSM 17613, ATCC 700100, CIP 109750, JCM 9731, kASUMI 6t, KCTC 3837, LMG 18001, CGMCC 1.3652, NCIMB 13460, Kasumi 6

Strain history: CIP <- 2007, DSMZ <- JCM <- T. Fujita <- M. Matsuhashi, Tokai Univ., Shizuoka, Japan: strain Kasumi 6

NCBI tax ID(s): 86663 (species)

SI-ID 10705

LMG 18001, ATCC 700100, JCM 9731, NCIMB 13460, KCTC 3837, KACC 10916, DSM 17613, CGMCC 1.3652, CIP 109750

Section

Bacillus carboniphilus DSM 17613 is an obligate aerobe, spore-forming, mesophilic bacterium that forms circular colonies and has a brown red pigmentation.

colony-forming
Gram-positive
motile
pigmented
rod-shaped
spore-forming
mesophilic
obligate aerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Bacillus*
	Species	**Bacillus carboniphilus**
	Full Scientific Name (LPSN)	Bacillus carboniphilus Fujita et al. 1996

Information on morphological and physiological properties Morphology

[Ref.: #23316]	Gram stain	positive
[Ref.: #67771]	Gram stain	positive
[Ref.: #117178]	Gram stain	positive

[Ref.: #23316]	Cell length	3.0-5.0 µm

[Ref.: #23316]	Cell width	0.5-0.9 µm

[Ref.: #23316]	Cell shape	rod-shaped
[Ref.: #117178]	Cell shape	rod-shaped
[Ref.: #67771]	Cell shape	rod-shaped

[Ref.: #23316]	Motility	yes
[Ref.: #117178]	Motility	no
[Ref.: #67771]	Motility	yes

[Ref.: #23316]	Flagellum arrangement	peritrichous
[Ref.: #67771]	Flagellum arrangement	peritrichous

[Ref.: #23316]	Colony color	grayish yellow
[Ref.: #23316]	Colony shape	circular
[Ref.: #23316]	Cultivation medium used	Nutrient agar

[Ref.: #23316]	Pigment production	yes
[Ref.: #23316]	Pigmentation color	brown red

Information on culture and growth conditions Culture and growth conditions

[Ref.: #7086]

Culture medium

CASO AGAR (MERCK 105458) (DSMZ Medium 220)

[Ref.: #7086]

Culture medium growth

[Ref.: #7086]

Culture medium link

Medium recipe at MediaDive

[Ref.: #7086]

Culture medium composition

expand / minimize

[Ref.: #23316]

Culture medium

Nutrient agar (NA)

[Ref.: #23316]

Culture medium growth

[Ref.: #36103]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #36103]

Culture medium growth

[Ref.: #36103]

Culture medium composition

expand / minimize

[Ref.: #117178]

Culture medium

CIP Medium 72

[Ref.: #117178]

Culture medium growth

[Ref.: #117178]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #7086]	growth	30 °C
[Ref.: #23316]	maximum	47 °C
[Ref.: #23316]	minimum	17 °C
[Ref.: #36103]	growth	30 °C
[Ref.: #67770]	growth	30 °C
[Ref.: #67771]	growth	30 °C

[Ref.: #7086]	Temperature range	mesophilic
[Ref.: #23316]	Temperature range	thermophilic
[Ref.: #23316]	Temperature range	psychrophilic
[Ref.: #36103]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #67771]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #23316]	Oxygen tolerance	obligate aerobe
[Ref.: #67771]	Oxygen tolerance	aerobe
[Ref.: #117178]	Oxygen tolerance	obligate aerobe

[Ref.: #23316]	Spore description	ellipsoidal, central or terminal, unswollen sporangia
[Ref.: #23316]	Type of spore	endospore
[Ref.: #23316]	Ability of spore formation	yes

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #23316]		NaCl		growth	7	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #23316]	2-oxoglutarate ChEBI	-	carbon source
[Ref.: #23316]	acetate ChEBI	-	carbon source
[Ref.: #23316]	alginate ChEBI	-	carbon source
[Ref.: #23316]	arginine ChEBI	-	degradation
[Ref.: #23316]	casein	+	hydrolysis
[Ref.: #23316]	cellobiose ChEBI	-	builds acid from
[Ref.: #23316]	citrate ChEBI	-	carbon source
[Ref.: #23316]	D-fructose ChEBI	-	builds acid from
[Ref.: #23316]	D-galactose ChEBI	-	builds acid from
[Ref.: #23316]	D-gluconate ChEBI	-	carbon source
[Ref.: #23316]	D-glucose ChEBI	-	builds acid from
[Ref.: #23316]	D-glucose ChEBI	-	builds gas from
[Ref.: #23316]	D-mannitol ChEBI	-	builds acid from
[Ref.: #23316]	D-mannitol ChEBI	-	builds gas from
[Ref.: #23316]	D-mannose ChEBI	-	builds acid from
[Ref.: #23316]	D-ribose ChEBI	-	builds acid from
[Ref.: #23316]	D-sorbose ChEBI	-	builds acid from
[Ref.: #23316]	D-xylose ChEBI	-	builds acid from
[Ref.: #23316]	D-xylose ChEBI	-	builds gas from
[Ref.: #23316]	erythritol ChEBI	-	builds acid from
[Ref.: #23316]	esculin ChEBI	-	hydrolysis
[Ref.: #23316]	fumarate ChEBI	-	carbon source
[Ref.: #23316]	gelatin ChEBI	+	hydrolysis
[Ref.: #23316]	glycerol ChEBI	-	builds acid from
[Ref.: #23316]	hippurate ChEBI	+	hydrolysis
[Ref.: #23316]	L-arabinose ChEBI	-	builds acid from
[Ref.: #23316]	L-arabinose ChEBI	-	builds gas from
[Ref.: #23316]	L-aspartate ChEBI	-	carbon source
[Ref.: #23316]	L-glutamate ChEBI	-	carbon source
[Ref.: #23316]	L-malate ChEBI	-	carbon source
[Ref.: #23316]	L-rhamnose ChEBI	-	builds acid from
[Ref.: #23316]	lactate ChEBI	-	carbon source
[Ref.: #23316]	lactose ChEBI	-	builds acid from
[Ref.: #23316]	lysine ChEBI	-	degradation
[Ref.: #23316]	malonate ChEBI	-	carbon source
[Ref.: #23316]	maltose ChEBI	-	builds acid from
[Ref.: #23316]	melibiose ChEBI	-	builds acid from
[Ref.: #23316]	myo-inositol ChEBI	-	builds acid from
[Ref.: #23316]	nitrate ChEBI	-	reduction
[Ref.: #117178]	nitrate ChEBI	+	reduction
[Ref.: #117178]	nitrite ChEBI	-	reduction
[Ref.: #23316]	ornithine ChEBI	-	degradation
[Ref.: #23316]	propionate ChEBI	-	carbon source
[Ref.: #23316]	pyruvate ChEBI	-	carbon source
[Ref.: #23316]	raffinose ChEBI	-	builds acid from
[Ref.: #23316]	ribitol ChEBI	-	builds acid from
[Ref.: #23316]	salicin ChEBI	-	builds acid from
[Ref.: #23316]	starch ChEBI	-	builds acid from
[Ref.: #23316]	starch ChEBI	+	hydrolysis
[Ref.: #23316]	succinate ChEBI	-	carbon source
[Ref.: #23316]	sucrose ChEBI	-	builds acid from
[Ref.: #23316]	tartrate ChEBI	-	carbon source
[Ref.: #23316]	trehalose ChEBI	-	builds acid from
[Ref.: #23316]	tween 80 ChEBI	+	hydrolysis
[Ref.: #23316]	urea ChEBI	-	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #23316]		acetoin ChEBI	no
[Ref.: #117178]		indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #23316]		acetoin ChEBI	-

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	+	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #23316]	beta-galactosidase	-	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #23316]	catalase	+	1.11.1.6
[Ref.: #117178]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	+	3.4.11.3	* from API zym
[Ref.: #23316]	cytochrome oxidase	+	1.9.3.1
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #117178]	oxidase	-
[Ref.: #68382]	trypsin	+	3.4.21.4	* from API zym
[Ref.: #117178]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #117178]	12086	-	+	+	+	-	+	+	+	+	+	+	+	-	-	-	+	-	+	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #7086]	Sample type/isolated from	Air
[Ref.: #7086]	Geographic location (country and/or sea, region)	Tokai University, Numazu
[Ref.: #7086]	Country	Japan
[Ref.: #7086]	Country ISO 3 Code	JPN
[Ref.: #7086]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Air
[Ref.: #67770]	Geographic location (country and/or sea, region)	Tokai University, Numazu, Shizuoka Pref.
[Ref.: #67770]	Country	Japan
[Ref.: #67770]	Country ISO 3 Code	JPN
[Ref.: #67770]	Continent	Asia

[Ref.: #67771]	Sample type/isolated from	From air
[Ref.: #67771]	Geographic location (country and/or sea, region)	Tokai University, Numazu, Shizuoka Pref.
[Ref.: #67771]	Country	Japan
[Ref.: #67771]	Country ISO 3 Code	JPN
[Ref.: #67771]	Continent	Asia

[Ref.: #117178]	Sample type/isolated from	Environment, Air
[Ref.: #117178]	Country	Japan
[Ref.: #117178]	Country ISO 3 Code	JPN
[Ref.: #117178]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental

#Air

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AB021182 (>99% sequence identity) for Bacillus carboniphilus from Microbeatlas

Aquatic Samples	9
Soil Samples	23
Animal Samples	13
Plant Samples	2
Total Samples	47

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #7086]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #117178]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #7086]	Bacillus carboniphilus gene for 16S ribosomal RNA	AB021182	1504	ENA	86663 tax ID

[Ref.: #7086]	GC-content	37.9 mol%
[Ref.: #23316]	GC-content	37.9 mol%	high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #7086]

Culture collection no.

DSM 17613, ATCC 700100, CIP 109750, JCM 9731, kASUMI 6t, KCTC 3837, LMG 18001, CGMCC 1.3652, NCIMB 13460, Kasumi 6

[Ref.: #70895]

SI-ID 10705

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Bacillus fonticola sp. nov., isolated from deep sea cold seep sediment.	Sun YY, Zhou HZ, Sun QL	Arch Microbiol	10.1007/s00203-021-02401-8	2021	*
Phylogeny	Bacillus oleivorans sp. nov., a diesel oil-degrading and solvent-tolerant bacterium.	Azmatunnisa M, Rahul K, Subhash Y, Sasikala C, Ramana CV	Int J Syst Evol Microbiol	10.1099/ijs.0.000103	2015	*
Phylogeny	Bacillus oceani sp. nov., a new slightly halophilic bacterium, isolated from a deep sea sediment environment.	Liu YJ, Long LJ, Huang XF, You ZQ, Wang FZ, Li J, Kim CJ, Tian XP, Zhang S	Antonie Van Leeuwenhoek	10.1007/s10482-013-9995-0	2013	*

References

#7086

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 17613

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#23316

TAKASHI FUJITA, OSAMU SHIDA, HIROAKI TAKAGI, KIYOHIKO KUNUGITA, A. N. PANKRUSHINA, MICHIO MATSUHASHI: Description of Bacillus carboniphilus sp. nov.. IJSEM 46: 116 - 118 1996 ( DOI 10.1099/00207713-46-1-116 )

#36103 ; Curators of the CIP;
#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#70895

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID10705.1 )

#117178 Collection of Institut Pasteur ; Curators of the CIP; CIP 109750
* These data were automatically processed and therefore are not curated

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