Alkalicoccus saliphilus (DSM 15402, ATCC BAA-957, 6AG)

Name: Alkalicoccus saliphilus (DSM 15402, ATCC BAA-957, 6AG)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 1235

Type strain

Strain Designation 6AG

Culture col. no. DSM 15402 ATCC BAA-957 6AG

NCBI tax ID(s) 200989

Special Collections DSMZ Literature strains

History DSMZ <- A. Gambacorta, ICB-CNR, Pozzuoli; 6AG <- I. Romano

Links

Alkalicoccus saliphilus 6AG is an aerobe, Gram-positive, coccus-shaped bacterium that was isolated from mud and algae green mat.

Gram-positive coccus-shaped aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 135105

ATCC BAA-957,DSM 15402

@ref 20215

Last LPSN update 2026-05-29 08:25:12

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Caryophanales

Family Bacillaceae

Genus Alkalicoccus

Species Alkalicoccus saliphilus

Full scientific name Alkalicoccus saliphilus (Romano et al. 2005) Zhao et al. 2017

Synonyms (1)

Bacillus saliphilus

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Motility
31319	positive	0.85 µm	0.85 µm	coccus-shaped
43205	positive			coccus-shaped

Colony morphology

43205

Colony coloryellow

Pigmentation

31319

Productionyes

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
5897	CASO AGAR (MERCK 105458) (DSMZ Medium 220)		Medium recipe at MediaDive	Name: CASO AGAR (Merck 105458) (DSMZ Medium 220; with strain-specific modifications) Composition: NaCl 160.0 g/l Agar 15.0 g/l Casein peptone 15.0 g/l Soy peptone 5.0 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
5897	positive	growth	37
31319	positive	growth	04-50
31319	positive	optimum	37
43205	positive	optimum	37
43205	positive	growth	10-50

Culture pH

@ref	Ability	Type	PH	PH range
31319	positive	optimum	9
43205	positive	optimum	9
31319	positive	growth	07-10	alkaliphile
43205	positive	growth	7.0-10.0

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance
31319	aerobe
43205	obligate aerobe

Spore formation

@ref	Type of spore	Spore formation
31319
43205	endospore

Halophily

@ref	Halophily level	Salt	Growth	Tested relation	Concentration
31319		NaCl	positive	growth	<26 %
31319		NaCl	positive	optimum	16 %
43205		NaCl	positive	growth	1-20 %(w/v)
43205	moderately halophilic	NaCl	positive	optimum	15 %(w/v)

Observation

31319

Observationaggregates in clumps

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
43205		casein	-	hydrolysis
43205	17057 ChEBI	cellobiose	-	builds acid from
43205	15824 ChEBI	D-fructose	+	builds acid from
43205	12936 ChEBI	D-galactose	+	builds acid from
43205	16024 ChEBI	D-mannose	+	builds acid from
43205	5291 ChEBI	gelatin	+	hydrolysis
31319	17234 ChEBI	glucose	+	carbon source
31319	17632 ChEBI	nitrate	+	reduction
43205	17632 ChEBI	nitrate	-	reduction
43205	28044 ChEBI	phenylalanine	+	degradation
43205	28017 ChEBI	starch	-	hydrolysis
43205	53426 ChEBI	tween 80	-	hydrolysis

Metabolite production

@ref	Chebi-ID	Metabolite
43205	15688 ChEBI	acetoin
43205	16136 ChEBI	hydrogen sulfide
43205	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test
43205	15688 ChEBI	acetoin	+

Enzymes

@ref	Value	Activity	Ec
31319	catalase	+	1.11.1.6
43205	catalase	+	1.11.1.6
31319	cytochrome oxidase	+	1.9.3.1
43205	cytochrome oxidase	+	1.9.3.1
31319	gelatinase	+

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	C4 and CAM-carbon fixation	100	8 of 8
66794	ceramide biosynthesis	100	1 of 1
66794	suberin monomers biosynthesis	100	2 of 2
66794	gluconeogenesis	100	8 of 8
66794	aspartate and asparagine metabolism	100	9 of 9
66794	threonine metabolism	100	10 of 10
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	sulfopterin metabolism	100	4 of 4
66794	coenzyme A metabolism	100	4 of 4
66794	formaldehyde oxidation	100	3 of 3
66794	ribulose monophosphate pathway	100	2 of 2
66794	lipoate biosynthesis	100	5 of 5
66794	adipate degradation	100	2 of 2
66794	methylglyoxal degradation	100	5 of 5
66794	folate polyglutamylation	100	1 of 1
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	valine metabolism	100	9 of 9
66794	aerobactin biosynthesis	100	1 of 1
66794	ppGpp biosynthesis	100	4 of 4
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	palmitate biosynthesis	95.45	21 of 22
66794	vitamin B1 metabolism	92.31	12 of 13
66794	pentose phosphate pathway	90.91	10 of 11
66794	Entner Doudoroff pathway	90	9 of 10
66794	chorismate metabolism	88.89	8 of 9
66794	molybdenum cofactor biosynthesis	88.89	8 of 9
66794	ketogluconate metabolism	87.5	7 of 8
66794	peptidoglycan biosynthesis	86.67	13 of 15
66794	reductive acetyl coenzyme A pathway	85.71	6 of 7
66794	citric acid cycle	85.71	12 of 14
66794	tetrahydrofolate metabolism	85.71	12 of 14
66794	heme metabolism	85.71	12 of 14
66794	photosynthesis	85.71	12 of 14
66794	leucine metabolism	84.62	11 of 13
66794	1,4-dihydroxy-6-naphthoate biosynthesis	83.33	5 of 6
66794	glutamate and glutamine metabolism	82.14	23 of 28
66794	glycogen metabolism	80	4 of 5
66794	cellulose degradation	80	4 of 5
66794	serine metabolism	77.78	7 of 9
66794	phenylalanine metabolism	76.92	10 of 13
66794	urea cycle	76.92	10 of 13
66794	glycolysis	76.47	13 of 17
66794	alanine metabolism	75.86	22 of 29
66794	purine metabolism	75.53	71 of 94
66794	isoleucine metabolism	75	6 of 8
66794	acetate fermentation	75	3 of 4
66794	butanoate fermentation	75	3 of 4
66794	CMP-KDO biosynthesis	75	3 of 4
66794	glycogen biosynthesis	75	3 of 4
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	75	6 of 8
66794	pyrimidine metabolism	73.33	33 of 45
66794	flavin biosynthesis	73.33	11 of 15
66794	methionine metabolism	73.08	19 of 26
66794	proline metabolism	72.73	8 of 11
66794	ubiquinone biosynthesis	71.43	5 of 7
66794	propanol degradation	71.43	5 of 7
66794	cardiolipin biosynthesis	71.43	5 of 7
66794	glutathione metabolism	71.43	10 of 14
66794	non-pathway related	71.05	27 of 38
66794	tryptophan metabolism	71.05	27 of 38
66794	propionate fermentation	70	7 of 10
66794	degradation of sugar alcohols	68.75	11 of 16
66794	octane oxidation	66.67	2 of 3
66794	selenocysteine biosynthesis	66.67	4 of 6
66794	acetoin degradation	66.67	2 of 3
66794	CO2 fixation in Crenarchaeota	66.67	6 of 9
66794	cyanate degradation	66.67	2 of 3
66794	L-lactaldehyde degradation	66.67	2 of 3
66794	methane metabolism	66.67	2 of 3
66794	oxidative phosphorylation	63.74	58 of 91
66794	d-xylose degradation	63.64	7 of 11
66794	arginine metabolism	62.5	15 of 24
66794	isoprenoid biosynthesis	61.54	16 of 26
66794	NAD metabolism	61.11	11 of 18
66794	cysteine metabolism	61.11	11 of 18
66794	vitamin K metabolism	60	3 of 5
66794	factor 420 biosynthesis	60	3 of 5
66794	phenylacetate degradation (aerobic)	60	3 of 5
66794	hydrogen production	60	3 of 5
66794	gallate degradation	60	3 of 5
66794	metabolism of amino sugars and derivatives	60	3 of 5
66794	starch degradation	60	6 of 10
66794	histidine metabolism	58.62	17 of 29
66794	lipid metabolism	58.06	18 of 31
66794	d-mannose degradation	55.56	5 of 9
66794	lysine metabolism	54.76	23 of 42
66794	ascorbate metabolism	54.55	12 of 22
66794	glycolate and glyoxylate degradation	50	3 of 6
66794	cis-vaccenate biosynthesis	50	1 of 2
66794	myo-inositol biosynthesis	50	5 of 10
66794	coenzyme M biosynthesis	50	5 of 10
66794	pantothenate biosynthesis	50	3 of 6
66794	aminopropanol phosphate biosynthesis	50	1 of 2
66794	biotin biosynthesis	50	2 of 4
66794	dTDPLrhamnose biosynthesis	50	4 of 8
66794	quinate degradation	50	1 of 2
66794	degradation of aromatic, nitrogen containing compounds	50	6 of 12
66794	toluene degradation	50	2 of 4
66794	phenylmercury acetate degradation	50	1 of 2
66794	dolichol and dolichyl phosphate biosynthesis	50	1 of 2
66794	ethanol fermentation	50	1 of 2
66794	3-phenylpropionate degradation	46.67	7 of 15
66794	degradation of pentoses	46.43	13 of 28
66794	sulfate reduction	46.15	6 of 13
66794	phenol degradation	45	9 of 20
66794	glycine betaine biosynthesis	40	2 of 5
66794	ethylmalonyl-CoA pathway	40	2 of 5
66794	glycine metabolism	40	4 of 10
66794	androgen and estrogen metabolism	37.5	6 of 16
66794	vitamin B6 metabolism	36.36	4 of 11
66794	cholesterol biosynthesis	36.36	4 of 11
66794	metabolism of disaccharids	36.36	4 of 11
66794	degradation of sugar acids	36	9 of 25
66794	polyamine pathway	34.78	8 of 23
66794	(5R)-carbapenem carboxylate biosynthesis	33.33	1 of 3
66794	enterobactin biosynthesis	33.33	1 of 3
66794	acetyl CoA biosynthesis	33.33	1 of 3
66794	arachidonic acid metabolism	33.33	6 of 18
66794	IAA biosynthesis	33.33	1 of 3
66794	nitrate assimilation	33.33	3 of 9
66794	allantoin degradation	33.33	3 of 9
66794	sphingosine metabolism	33.33	2 of 6
66794	lipid A biosynthesis	33.33	3 of 9
66794	vitamin B12 metabolism	32.35	11 of 34
66794	phenylpropanoid biosynthesis	30.77	4 of 13
66794	tyrosine metabolism	28.57	4 of 14
66794	chlorophyll metabolism	27.78	5 of 18
66794	degradation of hexoses	27.78	5 of 18
66794	cyclohexanol degradation	25	1 of 4
66794	lactate fermentation	25	1 of 4
66794	carnitine metabolism	25	2 of 8
66794	bile acid biosynthesis, neutral pathway	23.53	4 of 17
66794	carotenoid biosynthesis	22.73	5 of 22
66794	4-hydroxymandelate degradation	22.22	2 of 9
66794	daunorubicin biosynthesis	22.22	2 of 9

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Mud (Sludge)
#Host	#Algae	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
5897	mud and algae green mat	Malvizza, (AV)-Campania	Italy	ITA	Europe
43205	Algal mat

Taxonmaps

69479

Global distribution of 16S sequence AJ493660 (>99% sequence identity) for Alkalicoccus saliphilus subclade from Microbeatlas

Aquatic Samples	12
Soil Samples	5
Animal Samples	82
Plant Samples	2
Total Samples	101

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
5897	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM304406v1 assembly for Alkalicoccus saliphilus 6AG	scaffold	GCA_003044065	200989.3	2889707501	200989	61.37

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
5897	Bacillus sp. 6AG 16S rRNA gene, strain 6AG	AJ493660	1524		200989

GC content

@ref	GC-content (mol%)
5897	48.4
43205	48.8

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM304406v1 assembly for Alkalicoccus saliphilus 6AG
NCBI: GCA_003044065

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	facultative anaerobe	89.88	no
125439	gram_stain	BacteriaNetⓘ	variable	77.55	no
125439	motility	BacteriaNetⓘ	yes	63.94	no
125439	spore_formation	BacteriaNetⓘ	yes	65.00	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Alkalicoccus saliphilus 6AG
NCBI: GCA_003044065.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	83.17	no
125438	anaerobic	anaerobicⓘ	no	95.97	yes
125438	aerobic	aerobicⓘ	yes	82.98	yes
125438	spore-forming	spore-formingⓘ	no	50.09	no
125438	thermophilic	thermophileⓘ	no	92.83	yes
125438	flagellated	motile2+ⓘ	no	73.15	yes

Literature

Topic	Title	Authors	Journal	DOI	Year
Genetics	Draft Genome Sequence of Alkalicoccus saliphilus DSM 15402(T), a Haloalkaliphilic Bacterium Isolated from a Mineral Pool.	Qiu D, Liao Z, Lu W, Wang H, Li J, Zhao B	Microbiol Resour Announc	10.1128/MRA.00266-19	2019
Phylogeny	Reclassification of Bacillus saliphilus as Alkalicoccus saliphilus gen. nov., comb. nov., and description of Alkalicoccus halolimnae sp. nov., a moderately halophilic bacterium isolated from a salt lake.	Zhao B, Lu W, Zhang S, Liu K, Yan Y, Li J	Int J Syst Evol Microbiol	10.1099/ijsem.0.001759	2017
Phylogeny	Bacillus luteus sp. nov., isolated from soil.	Subhash Y, Sasikala C, Ramana CV	Int J Syst Evol Microbiol	10.1099/ijs.0.053504-0	2014
Phylogeny	Bacillus daliensis sp. nov., an alkaliphilic, Gram-positive bacterium isolated from a soda lake.	Zhai L, Liao T, Xue Y, Ma Y	Int J Syst Evol Microbiol	10.1099/ijs.0.031575-0	2011
Phylogeny	Bacillus saliphilus sp. nov., isolated from a mineral pool in Campania, Italy.	Romano I, Lama L, Nicolaus B, Gambacorta A, Giordano A	Int J Syst Evol Microbiol	10.1099/ijs.0.63298-0	2005

References

#5897	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 15402
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#27634	IJSEM 159 2005 ( DOI 10.1099/ijs.0.63298-0 , PubMed 15653870 )
#31319	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27634
#43205	Baisuo Zhao, Weidong Lu, Shanshan Zhang, Kang Liu, Yanchun Yan and Jun Li: Reclassification of Bacillus saliphilus as Alkalicoccus saliphilus gen. nov., comb. nov., and description of Alkalicoccus halolimnae sp. nov., a moderately halophilic bacterium isolated from a salt lake. IJSEM 67: 1557 - 1563 2017 ( DOI 10.1099/ijsem.0.001759 , PubMed 28032538 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Alkalicoccus saliphilus (DSM 15402, ATCC BAA-957, 6AG)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Pigmentation

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Observation

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

Pathway annotation

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_003044065

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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