Shouchella patagoniensis (DSM 16117, ATCC BAA-965, CIP 108678)

Name: Shouchella patagoniensis (DSM 16117, ATCC BAA-965, CIP 108678)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 1234

Type strain

Culture col. no. DSM 16117 ATCC BAA-965 CIP 108678 PAT 05

NCBI tax ID(s) 228576

Special Collections DSMZ CIP

History <- N. Olivera; PAT 05 CIP <- 2005, DSMZ <- N. Olivera, PROIMI, Tucuman, Argentina: strain PAT 05

Links

Shouchella patagoniensis DSM 16117 is an obligate aerobe, spore-forming, mesophilic prokaryote that was isolated from soil .

spore-forming Gram-positive rod-shaped obligate aerobe mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 135104

ATCC BAA-965,DSM 16117,CIP 108678,LMG 23070

@ref 20215

Last LPSN update 2026-02-09 11:18:03

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Caryophanales

Family Bacillaceae

Genus Shouchella

Species Shouchella patagoniensis

Full scientific name Shouchella patagoniensis (Olivera et al. 2005) Joshi et al. 2022

Synonyms (2)

Bacillus patagoniensis
Alkalihalobacillus patagoniensis

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility
121628	positive	rod-shaped

Colony morphology

121628

Hemolysis ability1

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
6303	CASO AGAR (MERCK 105458) (DSMZ Medium 220)	Medium recipe at MediaDive	Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water
38288	MEDIUM 72- for trypto casein soja agar		Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
121628	CIP Medium 72	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
6303	positive	growth	30	mesophilic
38288	positive	growth	30	mesophilic
121628	positive	growth	10-30
121628	negative	growth	37
121628	negative	growth	45
121628	negative	growth	55

Culture pH

@ref	Ability	Type	PH
121628		growth	6

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
121628	obligate aerobe
125439	facultative anaerobe	92

Spore formation

@ref	Spore formation	Confidence
121628
125438		92.556
125439		93.5

Halophily

@ref	Salt	Growth	Tested relation	Concentration
121628	NaCl	positive	growth	6-10 %
121628	NaCl		growth	0 %
121628	NaCl		growth	2 %
121628	NaCl		growth	4 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
121628	16947 ChEBI	citrate	-	carbon source
121628	4853 ChEBI	esculin	+	hydrolysis
121628	606565 ChEBI	hippurate	-	hydrolysis
121628	17632 ChEBI	nitrate	-	reduction
121628	17632 ChEBI	nitrate	-	respiration
121628	16301 ChEBI	nitrite	-	reduction

Metabolite production

@ref	Chebi-ID	Metabolite	Production
121628	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Methylred-test
121628	15688 ChEBI	acetoin	-
121628	17234 ChEBI	glucose		-

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	-	3.1.3.2	from API zym
121628	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
121628	amylase	-
68382	beta-galactosidase	-	3.2.1.23	from API zym
121628	beta-galactosidase	+	3.2.1.23
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
121628	caseinase	-	3.4.21.50
121628	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
121628	DNase	+
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
121628	gamma-glutamyltransferase	+	2.3.2.2
121628	gelatinase	+
121628	lecithinase	-
68382	leucine arylamidase	+	3.4.11.1	from API zym
121628	lipase	-
68382	lipase (C 14)	-		from API zym
121628	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
121628	ornithine decarboxylase	-	4.1.1.17
121628	oxidase	+
68382	trypsin	-	3.4.21.4	from API zym
121628	tween esterase	-
121628	urease	-	3.5.1.5
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
121628	-	-	+	+	-	+	-	-	-	-	-	+	-	-	-	-	-	-	-	-

API biotype100

@ref	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
121628	not determinedn.d.	+	+	-	+	+	-	+	+	+	-	+	-	-	-	-	+	+	+	-	+	-	-	+	+	-	+	-	-	-	-	-	+	-	+	+	+	+	-	-	-	-	+	-	-	-	-	-	+	+	+	+	-	-	+	-	-	+	-	-	+	+	-	-	-	+	-	-	-	-	-	-	-	-	-	-	+	-	-	+	+	+	-	-	-	-	-	+	-	+	-	-	-	+	+	-	-	+	+	+

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Soil
#Host	#Plants	#Shrub (Scrub)
#Host Body-Site	#Plant	#Rhizosphere

Isolation

@ref	Sample type	Host species	Geographic location	Country	Country ISO 3 Code	Continent
6303	soil (rhizosphere of shrub Atriplex lampa)	Atriplex lampa	north-eastern Patagonia	Argentina	ARG	Middle and South America
121628	Environment, Soil, rhizosphere of the perennial shrub Atriplex lampa		North eastern Patagonia	Argentina	ARG	Middle and South America

Taxonmaps

69479

Global distribution of 16S sequence AY258614 (>99% sequence identity) for Alkalihalobacillus patagoniensis subclade from Microbeatlas

Aquatic Samples	34
Soil Samples	337
Animal Samples	158
Plant Samples	58
Total Samples	587

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
6303	1	Risk group (German classification) According to TRBA 466
121628	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM201970v1 assembly for Shouchella patagoniensis DSM 16117	scaffold	GCA_002019705	228576.3	2916853388	228576	77.17

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
6303	Bacillus patagoniensis strain PAT 05 16S ribosomal RNA gene, partial sequence	AY258614	1402		228576

GC content

@ref	GC-content (mol%)	Method
6303	39.7	high performance liquid chromatography (HPLC)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Shouchella patagoniensis DSM 16117
NCBI: GCA_002019705

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	93.50	no
125439	motility	BacteriaNetⓘ	yes	89.90	no
125439	gram_stain	BacteriaNetⓘ	variable	76.20	no
125439	oxygen_tolerance	BacteriaNetⓘ	facultative anaerobe	92.00	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Alkalihalobacillus patagoniensis DSM 16117
NCBI: GCA_002019705.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	81.01	no
125438	anaerobic	anaerobicⓘ	no	95.43	yes
125438	aerobic	aerobicⓘ	yes	77.24	yes
125438	spore-forming	spore-formingⓘ	yes	92.56	no
125438	thermophilic	thermophileⓘ	no	92.29	yes
125438	flagellated	motile2+ⓘ	yes	80.74	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Characterization of isolates used in bacterial-based strategies for accelerated carbonation of lime mortars.	Grosso Giordano F, Marien Q, De Belie N, Rodriguez-Navarro C, Boon N.	Appl Environ Microbiol	10.1128/aem.00683-25	2025
Enzymology	Proteomic analysis and optimized production of Alkalihalobacillus patagoniensis PAT 05(T) extracellular proteases.	Olivera NL, Sequeiros C, Iglesias M, Nievas M	Bioprocess Biosyst Eng	10.1007/s00449-020-02436-z	2020
	Alkalihalobacterium elongatum gen. nov. sp. nov.: An Antibiotic-Producing Bacterium Isolated From Lonar Lake and Reclassification of the Genus Alkalihalobacillus Into Seven Novel Genera.	Joshi A, Thite S, Karodi P, Joseph N, Lodha T.	Front Microbiol	10.3389/fmicb.2021.722369	2021
Phylogeny	Bacillus miscanthi sp. nov., a alkaliphilic bacterium from the rhizosphere of Miscanthus sacchariflorus.	Shin B, Park C, Lee BH, Lee KE, Park W	Int J Syst Evol Microbiol	10.1099/ijsem.0.003982	2020
Phylogeny	Bacillus lonarensis sp. nov., an alkalitolerant bacterium isolated from a soda lake.	Reddy SV, Thirumala M, Farooq M, Sasikala C, Ramana CV	Arch Microbiol	10.1007/s00203-014-1040-9	2014
Phylogeny	Bacillus shacheensis sp. nov., a moderately halophilic bacterium isolated from a saline-alkali soil.	Lei Z, Qiu P, Ye R, Tian J, Liu Y, Wang L, Tang SK, Li WJ, Tian Y	J Gen Appl Microbiol	DN/JST.JSTAGE/jgam/60.101	2014
Phylogeny	Bacillus patagoniensis sp. nov., a novel alkalitolerant bacterium from the rhizosphere of Atriplex lampa in Patagonia, Argentina.	Olivera N, Sineriz F, Breccia JD	Int J Syst Evol Microbiol	10.1099/ijs.0.63348-0	2005

References

#6303	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 16117
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#38288	; Curators of the CIP;
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#121628	Collection of Institut Pasteur ; Curators of the CIP; CIP 108678
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Shouchella patagoniensis (DSM 16117, ATCC BAA-965, CIP 108678)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API zym

API biotype100

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_002019705

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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