Name: Shouchella patagoniensis DSM 16117
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 1234

Type strain:

Species: Shouchella patagoniensis

Culture col. no.: DSM 16117, ATCC BAA-965, CIP 108678, PAT 05

Strain history: CIP <- 2005, DSMZ <- N. Olivera, PROIMI, Tucuman, Argentina: strain PAT 05

NCBI tax ID(s): 228576 (species)

SI-ID 135104

ATCC BAA-965, DSM 16117, CIP 108678, LMG 23070

Section

Shouchella patagoniensis DSM 16117 is an obligate aerobe, spore-forming, mesophilic bacterium that was isolated from soil .

motile
spore-forming
mesophilic
obligate aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Shouchella*
	Species	**Shouchella patagoniensis**
	Full Scientific Name (LPSN)	Shouchella patagoniensis (Olivera et al. 2005) Joshi et al. 2022

	Synonym	Alkalihalobacillus patagoniensis
	Synonym	Bacillus patagoniensis

Information on morphological and physiological properties Morphology

[Ref.: #121628]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #121628]	Cell shape	rod-shaped

[Ref.: #121628]	Motility	no
[Ref.: #69480]	Motility	yes Confidence in %91.185

[Ref.: #121628]

Hemolysis ability

Information on culture and growth conditions Culture and growth conditions

[Ref.: #6303]

Culture medium

CASO AGAR (MERCK 105458) (DSMZ Medium 220)

[Ref.: #6303]

Culture medium growth

[Ref.: #6303]

Culture medium link

Medium recipe at MediaDive

[Ref.: #6303]

Culture medium composition

expand / minimize

[Ref.: #38288]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #38288]

Culture medium growth

[Ref.: #38288]

Culture medium composition

expand / minimize

[Ref.: #121628]

Culture medium

CIP Medium 72

[Ref.: #121628]

Culture medium growth

[Ref.: #121628]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #6303]		growth	30 °C
[Ref.: #38288]		growth	30 °C
[Ref.: #121628]		growth	10-30 °C
[Ref.: #121628]	no	growth	37 °C
[Ref.: #121628]	no	growth	45 °C
[Ref.: #121628]	no	growth	55 °C

[Ref.: #6303]	Temperature range	mesophilic
[Ref.: #38288]	Temperature range	mesophilic
[Ref.: #121628]	Temperature range	mesophilic
[Ref.: #121628]	Temperature range	thermophilic

	pH	Kind of pH		pH
[Ref.: #121628]		no	growth	6

Information on physiology and metabolism Physiology and metabolism

[Ref.: #121628]

Oxygen tolerance

obligate aerobe

[Ref.: #121628]	Ability of spore formation	yes
[Ref.: #69481]	Ability of spore formation	yes Confidence in %100
[Ref.: #69480]	Ability of spore formation	yes Confidence in %100

	Salt	Tested relation		Salt conc.
[Ref.: #121628]	NaCl		growth	6-10	%
[Ref.: #121628]	NaCl	no	growth	0	%
[Ref.: #121628]	NaCl	no	growth	2	%
[Ref.: #121628]	NaCl	no	growth	4	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #121628]	citrate ChEBI	-	carbon source
[Ref.: #121628]	esculin ChEBI	+	hydrolysis
[Ref.: #121628]	hippurate ChEBI	-	hydrolysis
[Ref.: #121628]	nitrate ChEBI	-	reduction
[Ref.: #121628]	nitrate ChEBI	-	respiration
[Ref.: #121628]	nitrite ChEBI	-	reduction

	Metabolite production	Metabolite	Production
[Ref.: #121628]		indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test	Methylred-test
[Ref.: #121628]		acetoin ChEBI	-
[Ref.: #121628]		glucose ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #121628]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #121628]	amylase	-
[Ref.: #121628]	beta-galactosidase	+	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #121628]	caseinase	-	3.4.21.50
[Ref.: #121628]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #121628]	DNase	+
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #121628]	gamma-glutamyltransferase	+	2.3.2.2
[Ref.: #121628]	gelatinase	+
[Ref.: #121628]	lecithinase	-
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #121628]	lipase	-
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #121628]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #121628]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #121628]	oxidase	+
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #121628]	tween esterase	-
[Ref.: #121628]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Biotype100:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
[Ref.: #121628]	2872	not determinedn.d.	+	+	-	+	+	-	+	+	+	-	+	-	-	-	-	+	+	+	-	+	-	-	+	+	-	+	-	-	-	-	-	+	-	+	+	+	+	-	-	-	-	+	-	-	-	-	-	+	+	+	+	-	-	+	-	-	+	-	-	+	+	-	-	-	+	-	-	-	-	-	-	-	-	-	-	+	-	-	+	+	+	-	-	-	-	-	+	-	+	-	-	-	+	+	-	-	+	+	+

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #121628]	11382	-	-	+	+	-	+	-	-	-	-	-	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #6303]	Sample type/isolated from	soil (rhizosphere of shrub Atriplex lampa)
[Ref.: #6303]	Host species	Atriplex lampa
[Ref.: #6303]	Geographic location (country and/or sea, region)	north-eastern Patagonia
[Ref.: #6303]	Country	Argentina
[Ref.: #6303]	Country ISO 3 Code	ARG
[Ref.: #6303]	Continent	Middle and South America

[Ref.: #121628]	Sample type/isolated from	Environment, Soil, rhizosphere of the perennial shrub Atriplex lampa
[Ref.: #121628]	Geographic location (country and/or sea, region)	North eastern Patagonia
[Ref.: #121628]	Country	Argentina
[Ref.: #121628]	Country ISO 3 Code	ARG
[Ref.: #121628]	Continent	Middle and South America
* marker position based on {}

Isolation sources categories

#Environmental	#Terrestrial	#Soil
#Host	#Plants	#Shrub (Scrub)
#Host Body-Site	#Plant	#Rhizosphere

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AY258614 (>99% sequence identity) for Alkalihalobacillus patagoniensis subclade from Microbeatlas

Aquatic Samples	34
Soil Samples	337
Animal Samples	158
Plant Samples	58
Total Samples	587

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #6303]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #121628]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #6303]	Bacillus patagoniensis strain PAT 05 16S ribosomal RNA gene, partial sequence	AY258614	1402	ENA	228576 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Shouchella patagoniensis DSM 16117	GCA_002019705	scaffold	GenBank	228576 tax ID	*
[Ref.: #66792]	Bacillus patagoniensis strain DSM 16117	228576.3	wgs	PATRIC	228576 tax ID	*
[Ref.: #66792]	Bacillus patagoniensis DSM 16117	2916853388	draft	JGI IMG/M	228576 tax ID	*

[Ref.: #6303]

GC-content

39.7 mol%

high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Alkalihalobacillus patagoniensis DSM 16117 NCBI: GCA_002019705.1
[Ref.: #69481]	spore-forming	yes	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Alkalihalobacillus patagoniensis DSM 16117 NCBI: GCA_002019705.1
[Ref.: #69480]	flagellated	yes	75.544	no
[Ref.: #69480]	gram-positive	yes	92.951	no
[Ref.: #69480]	anaerobic	no	99.053	no
[Ref.: #69480]	aerobic	yes	94.317	no
[Ref.: #69480]	halophile	yes	88.361	no
[Ref.: #69480]	spore-forming	yes	95.458	no
[Ref.: #69480]	glucose-util	yes	91.321	no
[Ref.: #69480]	thermophile	no	99.439	yes
[Ref.: #69480]	motile	yes	88.858	no
[Ref.: #69480]	glucose-ferment	no	89.864	no

Availability in culture collections External links

[Ref.: #6303]

Culture collection no.

DSM 16117, ATCC BAA-965, CIP 108678, PAT 05

[Ref.: #70890]

SI-ID 135104

Literature:

Topic	Title	Authors	Journal	DOI	Year
Enzymology	Proteomic analysis and optimized production of Alkalihalobacillus patagoniensis PAT 05(T) extracellular proteases.	Olivera NL, Sequeiros C, Iglesias M, Nievas M	Bioprocess Biosyst Eng	10.1007/s00449-020-02436-z	2020	*
Phylogeny	Bacillus miscanthi sp. nov., a alkaliphilic bacterium from the rhizosphere of Miscanthus sacchariflorus.	Shin B, Park C, Lee BH, Lee KE, Park W	Int J Syst Evol Microbiol	10.1099/ijsem.0.003982	2020	*
Phylogeny	Bacillus lonarensis sp. nov., an alkalitolerant bacterium isolated from a soda lake.	Reddy SV, Thirumala M, Farooq M, Sasikala C, Ramana CV	Arch Microbiol	10.1007/s00203-014-1040-9	2014	*
Phylogeny	Bacillus shacheensis sp. nov., a moderately halophilic bacterium isolated from a saline-alkali soil.	Lei Z, Qiu P, Ye R, Tian J, Liu Y, Wang L, Tang SK, Li WJ, Tian Y	J Gen Appl Microbiol	DN/JST.JSTAGE/jgam/60.101	2014	*
Phylogeny	Bacillus patagoniensis sp. nov., a novel alkalitolerant bacterium from the rhizosphere of Atriplex lampa in Patagonia, Argentina.	Olivera N, Sineriz F, Breccia JD	Int J Syst Evol Microbiol	10.1099/ijs.0.63348-0	2005	*

References

#6303

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 16117

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#38288 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#70890

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID135104.1 )

#121628 Collection of Institut Pasteur ; Curators of the CIP; CIP 108678
* These data were automatically processed and therefore are not curated

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Alkalihalobacillus patagoniensis DSM 16117

Alkalihalobacillus patagoniensis DSM 16117

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