Pseudalkalibacillus hwajinpoensis (DSM 16346, CIP 108766, JCM 12340)

Name: Pseudalkalibacillus hwajinpoensis (DSM 16346, CIP 108766, JCM 12340)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 1229

Type strain

Strain Designation JMM-4

Culture col. no. DSM 16346 CIP 108766 JCM 12340 JMM-4

NCBI tax ID(s) 208199

Special Collections DSMZ JCM CIP Literature strains

History DSMZ <- J. M. Santini, La Trobe University, Victoria, Australia; JMM-4

Links

Pseudalkalibacillus hwajinpoensis JMM-4 is an anaerobe, spore-forming, Gram-positive bacterium that was isolated from arsenic-contaminated mud.

spore-forming Gram-positive motile rod-shaped anaerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 134415

DSM 16346,JCM 12340,ATCC BAA-881,CIP 108766

@ref 20215

Last LPSN update 2026-05-29 08:25:12

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Caryophanales

Family Bacillaceae

Genus Pseudalkalibacillus

Species Pseudalkalibacillus hwajinpoensis

Full scientific name Pseudalkalibacillus hwajinpoensis (Yoon et al. 2004) Joshi et al. 2022

Synonyms (7)

Guptibacillus hwajinpoensis
Bacillus hwajinpoensis
Alkalihalobacillus macyae
Bacillus macyae
Alkalihalobacillus hwajinpoensis
"Anaerobacillus macyae"
"Pseudalkalibacillus macyae"

BacDive ID	Other strains from **Pseudalkalibacillus hwajinpoensis** (1)	Type strain
1226	P. hwajinpoensis SW-72, DSM 16206, JCM 11807, KCCM 41641, ... (type strain)

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Motility
31214	positive	2.75 µm	0.6 µm	rod-shaped

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
6397	CHRYSIOGENES MEDIUM (DSMZ Medium 818)		Medium recipe at MediaDive	Name: CHRYSIOGENES MEDIUM (DSMZ Medium 818; with strain-specific modifications) Composition: NaCl 1.19641 g/l Na-(L)-lactate 1.09671 g/l Yeast extract 0.797607 g/l NaHCO3 0.598205 g/l KNO3 0.498504 g/l MgCl2 x 6 H2O 0.398804 g/l NH4Cl 0.299103 g/l KCl 0.299103 g/l Na2SO4 0.299103 g/l KH2PO4 0.199402 g/l CaCl2 x 2 H2O 0.149551 g/l Na2-EDTA x 2 H2O 0.00518445 g/l FeCl2 x 4 H2O 0.00149551 g/l Sodium resazurin 0.000498504 g/l Pyridoxine hydrochloride 0.000299103 g/l Thiamine-HCl x 2 H2O 0.000199402 g/l Nicotinic acid 0.000199402 g/l CoCl2 x 6 H2O 0.000189432 g/l MnCl2 x 4 H2O 9.97009e-05 g/l Calcium pantothenate 9.97009e-05 g/l Vitamin B12 9.97009e-05 g/l p-Aminobenzoic acid 7.97607e-05 g/l ZnCl2 6.97906e-05 g/l Riboflavin 4.98504e-05 g/l Thiamine HCl 4.98504e-05 g/l Calcium D-(+)-pantothenate 4.98504e-05 g/l (DL)-alpha-Lipoic acid 4.98504e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l D-(+)-biotin 1.99402e-05 g/l Biotin 1.99402e-05 g/l Folic acid 1.99402e-05 g/l H3BO3 5.98205e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
6397	positive	growth	30
31214	positive	growth	28-37
31214	positive	optimum	32.5

Culture pH

@ref	Ability	Type	PH
31214	positive	optimum	7.8
31214	positive	growth	7-8.4

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
6397	anaerobe
31214	anaerobe
125439	obligate aerobe	98.151

Spore formation

@ref	Spore formation	Confidence
31214
125438		95.239

Halophily

@ref	Salt	Growth	Tested relation	Concentration
31214	NaCl	positive	growth	1.2-30 %
31214	NaCl	positive	optimum	1.2 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
31214	30089 ChEBI	acetate	+	carbon source
31214	29987 ChEBI	glutamate	+	carbon source
31214	24996 ChEBI	lactate	+	carbon source
31214	17632 ChEBI	nitrate	+	reduction
31214	15361 ChEBI	pyruvate	+	carbon source
31214	30031 ChEBI	succinate	+	carbon source

Enzymes

@ref	Value	Activity	Ec
31214	catalase	+	1.11.1.6

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	starch degradation	100	10 of 10
66794	gluconeogenesis	100	8 of 8
66794	C4 and CAM-carbon fixation	100	8 of 8
66794	ribulose monophosphate pathway	100	2 of 2
66794	methylglyoxal degradation	100	5 of 5
66794	cis-vaccenate biosynthesis	100	2 of 2
66794	ethanol fermentation	100	2 of 2
66794	aspartate and asparagine metabolism	100	9 of 9
66794	acetate fermentation	100	4 of 4
66794	palmitate biosynthesis	100	22 of 22
66794	valine metabolism	100	9 of 9
66794	lipoate biosynthesis	100	5 of 5
66794	vitamin K metabolism	100	5 of 5
66794	coenzyme A metabolism	100	4 of 4
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	aerobactin biosynthesis	100	1 of 1
66794	tetrahydrofolate metabolism	100	14 of 14
66794	folate polyglutamylation	100	1 of 1
66794	sulfopterin metabolism	100	4 of 4
66794	suberin monomers biosynthesis	100	2 of 2
66794	adipate degradation	100	2 of 2
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	teichoic acid biosynthesis	100	1 of 1
66794	vitamin B1 metabolism	92.31	12 of 13
66794	pentose phosphate pathway	90.91	10 of 11
66794	Entner Doudoroff pathway	90	9 of 10
66794	threonine metabolism	90	9 of 10
66794	propionate fermentation	90	9 of 10
66794	pyrimidine metabolism	88.89	40 of 45
66794	chorismate metabolism	88.89	8 of 9
66794	molybdenum cofactor biosynthesis	88.89	8 of 9
66794	CO2 fixation in Crenarchaeota	88.89	8 of 9
66794	glycolysis	88.24	15 of 17
66794	alanine metabolism	86.21	25 of 29
66794	photosynthesis	85.71	12 of 14
66794	glutamate and glutamine metabolism	85.71	24 of 28
66794	reductive acetyl coenzyme A pathway	85.71	6 of 7
66794	heme metabolism	85.71	12 of 14
66794	leucine metabolism	84.62	11 of 13
66794	cellulose degradation	80	4 of 5
66794	flavin biosynthesis	80	12 of 15
66794	peptidoglycan biosynthesis	80	12 of 15
66794	glycogen metabolism	80	4 of 5
66794	citric acid cycle	78.57	11 of 14
66794	NAD metabolism	77.78	14 of 18
66794	serine metabolism	77.78	7 of 9
66794	urea cycle	76.92	10 of 13
66794	purine metabolism	76.6	72 of 94
66794	biotin biosynthesis	75	3 of 4
66794	glycogen biosynthesis	75	3 of 4
66794	ppGpp biosynthesis	75	3 of 4
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	75	6 of 8
66794	butanoate fermentation	75	3 of 4
66794	isoleucine metabolism	75	6 of 8
66794	non-pathway related	73.68	28 of 38
66794	methionine metabolism	73.08	19 of 26
66794	proline metabolism	72.73	8 of 11
66794	cardiolipin biosynthesis	71.43	5 of 7
66794	propanol degradation	71.43	5 of 7
66794	tryptophan metabolism	71.05	27 of 38
66794	myo-inositol biosynthesis	70	7 of 10
66794	phenylalanine metabolism	69.23	9 of 13
66794	enterobactin biosynthesis	66.67	2 of 3
66794	cyanate degradation	66.67	2 of 3
66794	methane metabolism	66.67	2 of 3
66794	d-mannose degradation	66.67	6 of 9
66794	L-lactaldehyde degradation	66.67	2 of 3
66794	formaldehyde oxidation	66.67	2 of 3
66794	glycolate and glyoxylate degradation	66.67	4 of 6
66794	selenocysteine biosynthesis	66.67	4 of 6
66794	acetoin degradation	66.67	2 of 3
66794	acetyl CoA biosynthesis	66.67	2 of 3
66794	histidine metabolism	65.52	19 of 29
66794	isoprenoid biosynthesis	65.38	17 of 26
66794	glutathione metabolism	64.29	9 of 14
66794	dTDPLrhamnose biosynthesis	62.5	5 of 8
66794	ketogluconate metabolism	62.5	5 of 8
66794	arginine metabolism	62.5	15 of 24
66794	oxidative phosphorylation	60.44	55 of 91
66794	phenylacetate degradation (aerobic)	60	3 of 5
66794	metabolism of amino sugars and derivatives	60	3 of 5
66794	glycine betaine biosynthesis	60	3 of 5
66794	coenzyme M biosynthesis	60	6 of 10
66794	cysteine metabolism	55.56	10 of 18
66794	lipid metabolism	54.84	17 of 31
66794	lysine metabolism	52.38	22 of 42
66794	toluene degradation	50	2 of 4
66794	pantothenate biosynthesis	50	3 of 6
66794	aminopropanol phosphate biosynthesis	50	1 of 2
66794	CMP-KDO biosynthesis	50	2 of 4
66794	dolichol and dolichyl phosphate biosynthesis	50	1 of 2
66794	quinate degradation	50	1 of 2
66794	phenylmercury acetate degradation	50	1 of 2
66794	3-phenylpropionate degradation	46.67	7 of 15
66794	sulfate reduction	46.15	6 of 13
66794	metabolism of disaccharids	45.45	5 of 11
66794	vitamin B6 metabolism	45.45	5 of 11
66794	androgen and estrogen metabolism	43.75	7 of 16
66794	tyrosine metabolism	42.86	6 of 14
66794	ubiquinone biosynthesis	42.86	3 of 7
66794	degradation of pentoses	42.86	12 of 28
66794	degradation of aromatic, nitrogen containing compounds	41.67	5 of 12
66794	carotenoid biosynthesis	40.91	9 of 22
66794	ascorbate metabolism	40.91	9 of 22
66794	glycine metabolism	40	4 of 10
66794	arachidonate biosynthesis	40	2 of 5
66794	gallate degradation	40	2 of 5
66794	degradation of hexoses	38.89	7 of 18
66794	degradation of sugar alcohols	37.5	6 of 16
66794	d-xylose degradation	36.36	4 of 11
66794	cholesterol biosynthesis	36.36	4 of 11
66794	vitamin B12 metabolism	35.29	12 of 34
66794	polyamine pathway	34.78	8 of 23
66794	lipid A biosynthesis	33.33	3 of 9
66794	IAA biosynthesis	33.33	1 of 3
66794	arachidonic acid metabolism	33.33	6 of 18
66794	nitrate assimilation	33.33	3 of 9
66794	(5R)-carbapenem carboxylate biosynthesis	33.33	1 of 3
66794	octane oxidation	33.33	1 of 3
66794	sulfoquinovose degradation	33.33	1 of 3
66794	4-hydroxymandelate degradation	33.33	3 of 9
66794	degradation of sugar acids	32	8 of 25
66794	phosphatidylethanolamine bioynthesis	30.77	4 of 13
66794	phenylpropanoid biosynthesis	30.77	4 of 13
66794	4-hydroxyphenylacetate degradation	30	3 of 10
66794	bile acid biosynthesis, neutral pathway	29.41	5 of 17
66794	dolichyl-diphosphooligosaccharide biosynthesis	27.27	3 of 11
66794	phenol degradation	25	5 of 20
66794	lactate fermentation	25	1 of 4
66794	alginate biosynthesis	25	1 of 4
66794	cyclohexanol degradation	25	1 of 4
66794	allantoin degradation	22.22	2 of 9

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Contamination	#Heavy metal
#Environmental	#Terrestrial	#Mud (Sludge)

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
6397	arsenic-contaminated mud	Bendigo, Victoria	Australia	AUS	Australia and Oceania

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
6397	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM103947v1 assembly for Guptibacillus hwajinpoensis DSM 16346	scaffold	GCA_001039475	157733.5	2630968384	157733	75.8

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
6397	Bacillus macyae strain JMM-4 16S ribosomal RNA gene, partial sequence	AY032601	1512		157733

GC content

6397

GC-content (mol%)37

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM103947v1 assembly for Guptibacillus hwajinpoensis DSM 16346
BV-BRC: 157733.5

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	98.15	no
125439	gram_stain	BacteriaNetⓘ	variable	58.93	no
125439	motility	BacteriaNetⓘ	yes	81.83	no
125439	spore_formation	BacteriaNetⓘ	yes	88.31	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Alkalihalobacillus macyae DSM 16346
NCBI: GCA_001039475.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	76.83	yes
125438	anaerobic	anaerobicⓘ	no	92.82	no
125438	aerobic	aerobicⓘ	yes	71.12	yes
125438	spore-forming	spore-formingⓘ	yes	95.24	no
125438	thermophilic	thermophileⓘ	no	89.23	yes
125438	flagellated	motile2+ⓘ	yes	60.81	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Phylogenomic studies and molecular markers clarifying the evolutionary relationships and classification of Pseudalkalibacillus species: proposal for the family Guptibacillaceae fam. nov. harbouring the genera Guptibacillus gen. nov. and Exobacillus gen. nov.	Bello S, Rudra B, Schellhorn HE.	Int J Syst Evol Microbiol	10.1099/ijsem.0.006757	2025
Enzymology	Carbohydrate-Active Enzymes of a Novel Halotolerant Alkalihalobacillus Species for Hydrolysis of Starch and Other Algal Polysaccharides.	Masasa M, Kushmaro A, Chernova H, Shashar N, Guttman L.	Microbiol Spectr	10.1128/spectrum.01078-22	2022
Genetics	Genome Sequence of Anaerobacillus macyae JMM-4T (DSM 16346), the First Genomic Information of the Newly Established Genus Anaerobacillus.	Wang JP, Liu B, Liu GH, Ge CB, Chen QQ, Zhu YJ, Chen Z	Genome Announc	10.1128/genomeA.00922-15	2015
	Alkalihalobacterium elongatum gen. nov. sp. nov.: An Antibiotic-Producing Bacterium Isolated From Lonar Lake and Reclassification of the Genus Alkalihalobacillus Into Seven Novel Genera.	Joshi A, Thite S, Karodi P, Joseph N, Lodha T.	Front Microbiol	10.3389/fmicb.2021.722369	2021
Phylogeny	Anaerobacillus isosaccharinicus sp. nov., an alkaliphilic bacterium which degrades isosaccharinic acid.	Bassil NM, Lloyd JR	Int J Syst Evol Microbiol	10.1099/ijsem.0.002721	2019
Phylogeny	Bacillus macyae sp. nov., an arsenate-respiring bacterium isolated from an Australian gold mine.	Santini JM, Streimann ICA, Hoven RNV	Int J Syst Evol Microbiol	10.1099/ijs.0.63059-0	2004

References

#6397	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 16346
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#27540	IJSEM 2241 2004 ( DOI 10.1099/ijs.0.63059-0 , PubMed 15545465 )
#31214	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27540
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Pseudalkalibacillus hwajinpoensis (DSM 16346, CIP 108766, JCM 12340)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Metabolite utilization

Enzymes

Pathway annotation

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_001039475

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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