Name: Neobacillus novalis IDA 3307
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 1216

Type strain:

Species: Neobacillus novalis

Strain Designation: IDA 3307, R-15439

Culture col. no.: DSM 15603, CIP 108805, LMG 21837, JCM 21709, IAM 15258, NBRC 102450

Strain history: IAM 15258 <-- LMG 21837 <-- J. Heyrman <-- A. Felske.

NCBI tax ID(s): 1349756 (strain), 220687 (species)

Section

Neobacillus novalis IDA 3307 is a facultative anaerobe, spore-forming, mesophilic bacterium that forms irregular colonies and has a light brown pigmentation.

colony-forming
Gram-positive
motile
pigmented
spore-forming
facultative anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Neobacillus*
	Species	**Neobacillus novalis**
	Full Scientific Name (PNU)	Neobacillus novalis (Heyrman et al. 2004) Patel and Gupta 2020

Synonym

Bacillus novalis

Information on morphological and physiological properties Morphology

[Ref.: #23312]	Gram stain	positive
[Ref.: #23312]	Cell length	0.6-1.2 µm
[Ref.: #23312]	Motility	yes

[Ref.: #23312]	Colony color	cream
[Ref.: #23312]	Colony shape	irregular
[Ref.: #23312]	Cultivation medium used	TSA

[Ref.: #23312]	Pigment production	yes
[Ref.: #23312]	Pigmentation color	light brown

Information on culture and growth conditions Culture and growth conditions

[Ref.: #5923]

Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

[Ref.: #5923]

Culture medium growth

[Ref.: #5923]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #5923]

Culture medium composition

expand / minimize

[Ref.: #23312]

Culture medium

Trypticase Soy Agar (TSA)

[Ref.: #23312]

Culture medium growth

[Ref.: #38317]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #38317]

Culture medium growth

[Ref.: #38317]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #5923]	growth	30 °C
[Ref.: #23312]	maximum	50-55 °C
[Ref.: #23312]	optimum	30-40 °C
[Ref.: #38317]	growth	30 °C
[Ref.: #67770]	growth	28 °C

[Ref.: #5923]	Temperature range	mesophilic
[Ref.: #23312]	Temperature range	thermophilic
[Ref.: #38317]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

		pH	pH range
[Ref.: #23312]	maximum	9.5-10.0
[Ref.: #23312]	minimum	4.0-5.0	acidophile
[Ref.: #23312]	optimum	7.0-9.0	alkaliphile

Information on physiology and metabolism Physiology and metabolism

[Ref.: #23312]

Oxygen tolerance

facultative anaerobe

[Ref.: #23312]	Spore description	ellispoidal, subterminal, occasionally paracentral, slightly swollen sporangia
[Ref.: #23312]	Type of spore	endospore
[Ref.: #23312]	Ability of spore formation	yes

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #23312]	2-dehydro-D-gluconate ChEBI	-	builds acid from
[Ref.: #23312]	5-dehydro-D-gluconate ChEBI	-	builds acid from
[Ref.: #23312]	amygdalin ChEBI	+/-	builds acid from
[Ref.: #23312]	arbutin ChEBI	-	builds acid from
[Ref.: #23312]	casein	+	hydrolysis
[Ref.: #23312]	cellobiose ChEBI	+/-	builds acid from
[Ref.: #23312]	citrate ChEBI	-	assimilation
[Ref.: #23312]	D-arabinose ChEBI	-	builds acid from
[Ref.: #23312]	D-arabitol ChEBI	-	builds acid from
[Ref.: #23312]	D-fructose ChEBI	+	builds acid from
[Ref.: #23312]	D-fucose ChEBI	-	builds acid from
[Ref.: #23312]	D-galactose ChEBI	+/-	builds acid from
[Ref.: #23312]	D-glucose ChEBI	+	builds acid from
[Ref.: #23312]	D-lyxose ChEBI	-	builds acid from
[Ref.: #23312]	D-mannitol ChEBI	+/-	builds acid from
[Ref.: #23312]	D-mannose ChEBI	+	builds acid from
[Ref.: #23312]	D-ribose ChEBI	-	builds acid from
[Ref.: #23312]	D-sorbitol ChEBI	+	builds acid from
[Ref.: #23312]	D-tagatose ChEBI	-	builds acid from
[Ref.: #23312]	D-trehalose ChEBI	+	builds acid from
[Ref.: #23312]	D-xylose ChEBI	+	builds acid from
[Ref.: #23312]	erythritol ChEBI	-	builds acid from
[Ref.: #23312]	esculin ChEBI	+	hydrolysis
[Ref.: #23312]	galactitol ChEBI	-	builds acid from
[Ref.: #23312]	gelatin ChEBI	+	hydrolysis
[Ref.: #23312]	gentiobiose ChEBI	+/-	builds acid from
[Ref.: #23312]	gluconate ChEBI	-	builds acid from
[Ref.: #23312]	glycerol ChEBI	-	builds acid from
[Ref.: #23312]	glycogen ChEBI	-	builds acid from
[Ref.: #23312]	inulin ChEBI	-	builds acid from
[Ref.: #23312]	L-arabinose ChEBI	-	builds acid from
[Ref.: #23312]	L-arabitol ChEBI	-	builds acid from
[Ref.: #23312]	L-fucose ChEBI	-	builds acid from
[Ref.: #23312]	L-rhamnose ChEBI	-	builds acid from
[Ref.: #23312]	L-sorbose ChEBI	-	builds acid from
[Ref.: #23312]	L-xylose ChEBI	-	builds acid from
[Ref.: #23312]	lactose ChEBI	-	builds acid from
[Ref.: #23312]	maltose ChEBI	+	builds acid from
[Ref.: #23312]	melezitose ChEBI	-	builds acid from
[Ref.: #23312]	melibiose ChEBI	-	builds acid from
[Ref.: #23312]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from
[Ref.: #23312]	methyl alpha-D-mannoside ChEBI	-	builds acid from
[Ref.: #23312]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from
[Ref.: #23312]	myo-inositol ChEBI	-	builds acid from
[Ref.: #23312]	N-acetylglucosamine ChEBI	+	builds acid from
[Ref.: #23312]	nitrate ChEBI	+	reduction
[Ref.: #23312]	raffinose ChEBI	-	builds acid from
[Ref.: #23312]	ribitol ChEBI	-	builds acid from
[Ref.: #23312]	salicin ChEBI	-	builds acid from
[Ref.: #23312]	starch ChEBI	-	builds acid from
[Ref.: #23312]	sucrose ChEBI	-	builds acid from
[Ref.: #23312]	turanose ChEBI	-	builds acid from
[Ref.: #23312]	xylitol ChEBI	-	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite
[Ref.: #23312]		acetoin ChEBI
[Ref.: #23312]		hydrogen sulfide ChEBI
[Ref.: #23312]		indole ChEBI

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #23312]		acetoin ChEBI	-

	Enzyme	Enzyme activity	EC number
[Ref.: #23312]	arginine dihydrolase	-	3.5.3.6
[Ref.: #23312]	beta-galactosidase	-	3.2.1.23
[Ref.: #23312]	lysine decarboxylase	-	4.1.1.18
[Ref.: #23312]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #23312]	tryptophan deaminase	-	4.1.99.1
[Ref.: #23312]	urease	-	3.5.1.5

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #23312]	Sample type/isolated from	soil, Drentse A agricultural research area
[Ref.: #23312]	Geographic location (country and/or sea, region)	Anlooer Diepje brook
[Ref.: #23312]	Country	Netherlands
[Ref.: #23312]	Country ISO 3 Code	NLD
[Ref.: #23312]	Continent	Europe
[Ref.: #23312]	Geographic location	53.0333°/6.6833°

[Ref.: #5923]	Sample type/isolated from	soil
[Ref.: #5923]	Geographic location (country and/or sea, region)	near Anloo
[Ref.: #5923]	Country	Netherlands
[Ref.: #5923]	Country ISO 3 Code	NLD
[Ref.: #5923]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Soil in the Drentse A agricultural research area along the Anlooër Diepje brook
[Ref.: #67770]	Country	Netherlands
[Ref.: #67770]	Country ISO 3 Code	NLD
[Ref.: #67770]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Engineered	#Agriculture	-
#Environmental	#Terrestrial	#Soil

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AJ542512 (>99% sequence identity) for Neobacillus from Microbeatlas

Aquatic Samples	4
Soil Samples	72
Animal Samples	4
Plant Samples	5
Total Samples	85

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #5923]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Bacillus novalis gene for 16S rRNA, partial sequence, strain: NBRC 102450	AB681792	1475	ENA	220687 tax ID	*
[Ref.: #23312]	Bacillus novalis partial 16S rRNA gene, strain LMG 21837	AJ542512	1503	GenBank	220687 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Bacillus novalis NBRC 102450	1349756.3	wgs	PATRIC	1349756 tax ID	*
[Ref.: #66792]	Bacillus novalis NBRC 102450	2681812999	draft	JGI IMG/M	1349756 tax ID	*
[Ref.: #67770]	Neobacillus novalis NBRC 102450 NBRC 102450	GCA_001591805	contig	GenBank	1349756 tax ID

[Ref.: #23312]

GC-content

40.5 mol%

high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #5923]

Culture collection no.

DSM 15603, CIP 108805, LMG 21837, JCM 21709, IAM 15258, NBRC 102450

[Ref.: #20218]

Associated Passport(s) in StrainInfo

372502, 691006, 295729

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Bacillus novalis sp. nov., Bacillus vireti sp. nov., Bacillus soli sp. nov., Bacillus bataviensis sp. nov. and Bacillus drentensis sp. nov., from the Drentse A grasslands.	Heyrman J, Vanparys B, Logan NA, Balcaen A, Rodriguez-Diaz M, Felske A, De Vos P	Int J Syst Evol Microbiol	10.1099/ijs.0.02723-0	2004	*
Phylogeny	Bacillus ginsengisoli sp. nov., isolated from soil of a ginseng field.	Nguyen NL, Kim YJ, Hoang VA, Min JW, Liang ZQ, Yang DC	Int J Syst Evol Microbiol	10.1099/ijs.0.031740-0	2012	*
Phylogeny	Bacillus abyssalis sp. nov., isolated from a sediment of the South China Sea.	You ZQ, Li J, Qin S, Tian XP, Wang FZ, Zhang S, Li WJ	Antonie Van Leeuwenhoek	10.1007/s10482-013-9875-7	2013	*
Phylogeny	Bacillus mesonae sp. nov., isolated from the root of Mesona chinensis.	Liu B, Liu GH, Hu GH, Chen MC	Int J Syst Evol Microbiol	10.1099/ijs.0.059485-0	2014	*
Phylogeny	Bacillus rigiliprofundi sp. nov., an endospore-forming, Mn-oxidizing, moderately halophilic bacterium isolated from deep subseafloor basaltic crust.	Sylvan JB, Hoffman CL, Momper LM, Toner BM, Amend JP, Edwards KJ	Int J Syst Evol Microbiol	10.1099/ijs.0.000211	2015	*
Phylogeny	Bacillus oryzisoli sp. nov., isolated from rice rhizosphere.	Zhang XX, Gao JS, Zhang L, Zhang CW, Ma XT, Zhang J	Int J Syst Evol Microbiol	10.1099/ijsem.0.001215	2016	*
Genetics	Draft Genome Sequences of Type Strains Bacillus drentensis DSM 15600T and Bacillus novalis DSM 15603T.	Liu B, Liu GH, Zhu YJ, Wang JP, Che JM, Chen QQ, Chen Z	Genome Announc	10.1128/genomeA.01423-16	2016	*

References

#5923

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 15603

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#23312

Jeroen Heyrman, Bram Vanparys, Niall A. Logan, An Balcaen, Marina Rodríguez-Díaz, Andreas Felske, Paul De Vos: Bacillus novalis sp. nov., Bacillus vireti sp. nov., Bacillus soli sp. nov., Bacillus bataviensis sp. nov. and Bacillus drentensis sp. nov., from the Drentse A grasslands. IJSEM 54: 47 - 57 2004 ( DOI 10.1099/ijs.0.02723-0 , PubMed 14742458 )

#38317 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

* These data were automatically processed and therefore are not curated

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