Name: Paenisporosarcina macmurdoensis DSM 15428
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 12008

Type strain:

Species: Paenisporosarcina macmurdoensis

Culture col. no.: DSM 15428, CIP 107784, MTCC 4670, CMS 21W, KCTC 3996, CCUG 54527

Strain history: CIP <- 2003, S. Shivaji, CCMB, Hyderabad, India: strain CMS 21w

NCBI tax ID(s): 212659 (species)

SI-ID 128019

CIP 107784, DSM 15428, CCUG 54527, KCTC 3996, CCUG 54629

Section

Paenisporosarcina macmurdoensis DSM 15428 is a facultative anaerobe, spore-forming, psychrophilic bacterium that was isolated from cyanobacterial mat sample.

Gram-negative
rod-shaped
spore-forming
facultative anaerobe
psychrophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Caryophanaceae*
	Genus	*Paenisporosarcina*
	Species	**Paenisporosarcina macmurdoensis**
	Full Scientific Name (LPSN)	Paenisporosarcina macmurdoensis (Reddy et al. 2003) Krishnamurthi et al. 2009

Synonym

Sporosarcina macmurdoensis

Information on morphological and physiological properties Morphology

[Ref.: #120738]	Gram stain	negative

[Ref.: #120738]	Cell shape	rod-shaped

[Ref.: #120738]	Motility	no

Information on culture and growth conditions Culture and growth conditions

[Ref.: #5816]

Culture medium

CASO AGAR (MERCK 105458) (DSMZ Medium 220)

[Ref.: #5816]

Culture medium growth

[Ref.: #5816]

Culture medium link

Medium recipe at MediaDive

[Ref.: #5816]

Culture medium composition

expand / minimize

[Ref.: #33166]

Culture medium

MEDIUM 338 - for Sanguibacter keddieii

[Ref.: #33166]

Culture medium growth

[Ref.: #33166]

Culture medium composition

expand / minimize

[Ref.: #120738]

Culture medium

CIP Medium 338

[Ref.: #120738]

Culture medium growth

[Ref.: #120738]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #5816]		growth	18 °C
[Ref.: #33166]		growth	18 °C
[Ref.: #120738]		growth	10-22 °C
[Ref.: #120738]	no	growth	30 °C
[Ref.: #120738]	no	growth	37 °C
[Ref.: #120738]	no	growth	45 °C
[Ref.: #120738]	no	growth	55 °C

[Ref.: #5816]	Temperature range	psychrophilic
[Ref.: #33166]	Temperature range	psychrophilic
[Ref.: #120738]	Temperature range	psychrophilic
[Ref.: #120738]	Temperature range	mesophilic
[Ref.: #120738]	Temperature range	thermophilic

	pH	Kind of pH		pH
[Ref.: #120738]		no	growth	6

Information on physiology and metabolism Physiology and metabolism

[Ref.: #120738]

Oxygen tolerance

facultative anaerobe

[Ref.: #120738]

Ability of spore formation

yes

	Salt	Tested relation		Salt conc.
[Ref.: #120738]	NaCl		growth	0	%
[Ref.: #120738]	NaCl	no	growth	2	%
[Ref.: #120738]	NaCl	no	growth	4	%
[Ref.: #120738]	NaCl	no	growth	6	%
[Ref.: #120738]	NaCl	no	growth	8	%
[Ref.: #120738]	NaCl	no	growth	10	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #120738]	citrate ChEBI	-	carbon source
[Ref.: #120738]	esculin ChEBI	+	hydrolysis
[Ref.: #120738]	hippurate ChEBI	+	hydrolysis
[Ref.: #120738]	nitrate ChEBI	+	reduction
[Ref.: #120738]	nitrite ChEBI	-	reduction

	Antibiotica	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #120738]		0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)	no	yes

	Metabolite production	Metabolite	Production
[Ref.: #120738]		indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test	Methylred-test
[Ref.: #120738]		acetoin ChEBI	-
[Ref.: #120738]		glucose ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #120738]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	+	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #120738]	amylase	+
[Ref.: #120738]	beta-galactosidase	-	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #120738]	caseinase	+	3.4.21.50
[Ref.: #120738]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #120738]	DNase	+
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #120738]	gamma-glutamyltransferase	-	2.3.2.2
[Ref.: #120738]	gelatinase	+/-
[Ref.: #120738]	lecithinase	-
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #120738]	lipase	-
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #120738]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #120738]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #120738]	oxidase	-
[Ref.: #120738]	protease	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #120738]	tween esterase	-
[Ref.: #120738]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #120738]	10644	-	+	+	-	-	+	-	-	-	+	+	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #5816]	Sample type/isolated from	cyanobacterial mat sample
[Ref.: #5816]	Geographic location (country and/or sea, region)	Mc Murdo dry Valley
[Ref.: #5816]	Continent	Australia and Oceania

[Ref.: #120738]	Sample type/isolated from	Cyanobacterial material samples
[Ref.: #120738]	Geographic location (country and/or sea, region)	McMurdo
[Ref.: #120738]	Country	Antarctica
[Ref.: #120738]	Country ISO 3 Code	ATA
[Ref.: #120738]	Continent	Antarctica
[Ref.: #120738]	Isolation date	1995
* marker position based on {}

Isolation sources categories

#Environmental	#Microbial community	#Microbial mat
#Host	#Microbial	#Bacteria

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AJ514408 (>99% sequence identity) for Planococcaceae from Microbeatlas

Aquatic Samples	1703
Soil Samples	5958
Animal Samples	625
Plant Samples	1720
Total Samples	10006

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #5816]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #120738]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #5816]	Sporosarcina macmurdoensis partial 16S rRNA gene, type strain CMS 21w	AJ514408	1483	ENA	212659 tax ID

[Ref.: #5816]

GC-content

44 mol%

Availability in culture collections External links

[Ref.: #5816]

Culture collection no.

DSM 15428, CIP 107784, MTCC 4670, CMS 21W, KCTC 3996, CCUG 54527

[Ref.: #81227]

SI-ID 128019

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Description of Paenisporosarcina quisquiliarum gen. nov., sp. nov., and reclassification of Sporosarcina macmurdoensis Reddy et al. 2003 as Paenisporosarcina macmurdoensis comb. nov.	Krishnamurthi S, Bhattacharya A, Mayilraj S, Saha P, Schumann P, Chakrabarti T	Int J Syst Evol Microbiol	10.1099/ijs.0.65130-0	2009	*
Phylogeny	Sporosarcina macmurdoensis sp. nov., from a cyanobacterial mat sample from a pond in the McMurdo Dry Valleys, Antarctica.	Reddy GSN, Matsumoto GI, Shivaji S	Int J Syst Evol Microbiol	10.1099/ijs.0.02628-0	2003	*

References

#5816

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 15428

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#33166 ; Curators of the CIP;

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#81227

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID128019.1 )

#120738 Collection of Institut Pasteur ; Curators of the CIP; CIP 107784
* These data were automatically processed and therefore are not curated

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