Pseudaminobacter defluvii (DSM 21572, CIP 107185, IAM 12817)

Name: Pseudaminobacter defluvii (DSM 21572, CIP 107185, IAM 12817)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 11879

Type strain

Strain Designation 66

Culture col. no. DSM 21572 CIP 107185 IAM 12817 IFO 14570 JCM 20757 4 more

NCBI tax ID(s) 93380

Special Collections DSMZ JCM KCTC CIP

Links

Pseudaminobacter defluvii 66 is an obligate aerobe, mesophilic, Gram-negative prokaryote that was isolated from activated sludge.

Gram-negative motile rod-shaped obligate aerobe mesophilic 16S sequence

Name and taxonomic classification

SI-ID 62615

IFO 14570,IAM 12817,KCTC 2844,KCTC 2849,CIP 107185,NCIMB 13842,NBRC 14570,JCM 20757,DSM 21572

@ref 20215

Last LPSN update 2025-12-16 09:49:42

Domain Pseudomonadati

Phylum Pseudomonadota

Class Alphaproteobacteria

Order Hyphomicrobiales

Family Phyllobacteriaceae

Genus Pseudaminobacter

Species Pseudaminobacter defluvii

Full scientific name Pseudaminobacter defluvii Kämpfer et al. 1999

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility
118871	negative	rod-shaped

Pigmentation

@ref	Production	Name
118871		Pyocyanin

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
15814	R2A MEDIUM (DSMZ Medium 830)	Medium recipe at MediaDive	Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
33681	MEDIUM 127 - for Streptomyces scabiei and Thiobacillus novellus		Distilled water make up to (1000.000 ml);Magnesium sulphate heptahydrate (0.100 g);Agar (15.000 g);Yeast extract (5.000 g);Potassium di-hydrogen phosphate (1.500 g);Ammonium chloride (0.300 g);Sodium thiosulphate pentahydrate (5.000 g);Di Sodium hydrogen
118871	CIP Medium 127	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
15814	positive	growth	28	mesophilic
33681	positive	growth	30	mesophilic
67770	positive	growth	30	mesophilic
118871	positive	growth	25-37	mesophilic
118871	negative	growth	5
118871	negative	growth	10
118871	negative	growth	41

Physiology and metabolism

Oxygen tolerance

118871

Oxygen toleranceobligate aerobe

Halophily

@ref	Salt	Growth	Tested relation	Concentration
118871	NaCl	positive	growth	0-4 %
118871	NaCl		growth	6 %
118871	NaCl		growth	8 %
118871	NaCl		growth	10 %

Observation

67770

Observationquinones: Q-10

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
118871	16947 ChEBI	citrate	-	carbon source
118871	4853 ChEBI	esculin	-	hydrolysis
118871	17632 ChEBI	nitrate	-	reduction
118871	17632 ChEBI	nitrate	-	respiration
118871	16301 ChEBI	nitrite	-	reduction

Antibiotic resistance

@ref	Metabolite	Is antibiotic	Is sensitive	Is resistant
118871	0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)

Metabolite production

@ref	Chebi-ID	Metabolite	Production
118871	35581 ChEBI	indole

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	-	3.1.3.2	from API zym
118871	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
118871	amylase	-
68382	beta-galactosidase	-	3.2.1.23	from API zym
118871	beta-galactosidase	-	3.2.1.23
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
118871	caseinase	-	3.4.21.50
118871	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
118871	DNase	-
68382	esterase (C 4)	-		from API zym
68382	esterase lipase (C 8)	-		from API zym
118871	gelatinase	-
118871	lecithinase	+
68382	leucine arylamidase	+	3.4.11.1	from API zym
118871	lipase	-
68382	lipase (C 14)	-		from API zym
118871	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	-		from API zym
118871	ornithine decarboxylase	-	4.1.1.17
118871	oxidase	+
118871	protease	-
68382	trypsin	+	3.4.21.4	from API zym
118871	tryptophan deaminase	+
118871	tween esterase	+
118871	urease	-	3.5.1.5
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
118871	-	-	-	-	-	+	-	-	+	-	-	-	-	-	-	-	-	-	-	-

API biotype100

@ref	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
118871	not determinedn.d.	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	+	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Waste	#Activated sludge

Isolation

@ref	Sample type	Country	Country ISO 3 Code	Continent
15814	activated sludge	Japan	JPN	Asia
67770	Activated sludge
118871	Environment, Activated sludge

Taxonmaps

69479

Global distribution of 16S sequence D32248 (>99% sequence identity) for Mesorhizobium from Microbeatlas

Aquatic Samples	12
Soil Samples	9
Animal Samples	10
Plant Samples	6
Total Samples	37

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
15814	1	Risk group (German classification) According to TRBA 466
118871	1	Risk group (French classification) According to TRBA 466

Sequence information

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	Pseudaminobacter defluvii gene for 16S rRNA, partial sequence, strain: NBRC 14570	AB680636	1409		93380
15814	Pseudaminobacter defluvii gene for 16S rRNA, partial sequence, strain: THI 051	D32248	1406		93380

GC content

@ref	GC-content (mol%)	Method
67770	62.9	high performance liquid chromatography (HPLC)

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Pseudaminobacter arsenicus sp. nov., an arsenic-resistant bacterium isolated from arsenic-rich aquifers.	Mu Y, Zhou X, Liu L, Zhou XK, Zeng XC, Li WJ	Int J Syst Evol Microbiol	10.1099/ijsem.0.003238	2019
Phylogeny	Pseudaminobacter granuli sp. nov., isolated from granules used in a wastewater treatment plant.	Hahn YK, Kim MS, Im WT	J Microbiol	10.1007/s12275-017-7257-y	2017
Phylogeny	Pseudaminobacter manganicus sp. nov., isolated from sludge of a manganese mine.	Li J, Huang J, Liao S, Wang G	Int J Syst Evol Microbiol	10.1099/ijsem.0.001765	2017
Phylogeny	Description of Pseudaminobacter gen. nov. with two new species, Pseudaminobacter salicylatoxidans sp. nov. and Pseudaminobacter defluvii sp. nov.	Kampfer P, Muller C, Mau M, Neef A, Auling G, Busse HJ, Osborn AM, Stolz A	Int J Syst Bacteriol	10.1099/00207713-49-2-887	1999

References

#15814	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 21572
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#33681	; Curators of the CIP;
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#118871	Collection of Institut Pasteur ; Curators of the CIP; CIP 107185
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Pseudaminobacter defluvii (DSM 21572, CIP 107185, IAM 12817)

Name and taxonomic classification

Morphology

Cell morphology

Pigmentation

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Halophily

Observation

Metabolite utilization

Antibiotic resistance

Metabolite production

Enzymes

API zym

API biotype100

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

16S sequences

GC content

Genome-based predictions

Literature

Literature

References

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