Pelotomaculum schinkii (DSM 15200, ATCC BAA-615, HH)

Cell morphology

Colony morphology

Multimedia

• @ref:	5753
  multimedia content:	DSM_15200.jpg
  multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_15200.jpg
  caption:	DSM 15200 Pelotomaculum schinkii
  intellectual property rights:	Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH
  manual_annotation:	1

Culture medium

@ref	Name	Growth	Medium link	Composition
5753	PELOTOMACULUM MEDIUM (DSMZ Medium 960)		Medium recipe at MediaDive	Name: PELOTOMACULUM MEDIUM (DSMZ Medium 960; with strain-specific modifications) Composition: Na-propionate 1.99601 g/l Na2CO3 1.49701 g/l NH4Cl 0.538922 g/l Na2S x 9 H2O 0.299401 g/l L-Cysteine HCl x H2O 0.299401 g/l MgCl2 x 6 H2O 0.199601 g/l KH2PO4 0.139721 g/l Yeast extract 0.0998004 g/l Nitrilotriacetic acid 0.0127745 g/l NaCl 0.000998004 g/l FeCl2 x 4 H2O 0.000998004 g/l Sodium resazurin 0.000499002 g/l NiCl2 x 6 H2O 9.98004e-05 g/l ZnCl2 9.98004e-05 g/l MnCl2 x 4 H2O 9.98004e-05 g/l CaCl2 x 2 H2O 9.98004e-05 g/l Pyridoxine hydrochloride 9.98004e-05 g/l p-Aminobenzoic acid 4.99002e-05 g/l (DL)-alpha-Lipoic acid 4.99002e-05 g/l Thiamine HCl 4.99002e-05 g/l Riboflavin 4.99002e-05 g/l Nicotinic acid 4.99002e-05 g/l Calcium D-(+)-pantothenate 4.99002e-05 g/l Na2WO4 x 2 H2O 3.99202e-05 g/l CoCl2 x 6 H2O 2.99401e-05 g/l Na2MoO4 x 2 H2O 2.99401e-05 g/l Na2SeO3 x 5 H2O 2.99401e-05 g/l CuCl2 1.99601e-05 g/l Biotin 1.99601e-05 g/l Folic acid 1.99601e-05 g/l H3BO3 9.98004e-06 g/l Vitamin B12 9.98004e-07 g/l Distilled water

Culture temp

Culture pH

Oxygen tolerance

Spore formation

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
30047	15740 ChEBI	formate	+	carbon source
30047	17272 ChEBI	propionate	+	carbon source

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions	external links
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	C4 and CAM-carbon fixation	100	8 of 8
66794	sulfopterin metabolism	100	4 of 4
66794	coenzyme A metabolism	100	4 of 4
66794	glycogen metabolism	100	5 of 5
66794	cis-vaccenate biosynthesis	100	2 of 2
66794	reductive acetyl coenzyme A pathway	100	7 of 7
66794	L-lactaldehyde degradation	100	3 of 3
66794	hydrogen production	100	5 of 5
66794	palmitate biosynthesis	100	22 of 22
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	ppGpp biosynthesis	100	4 of 4
66794	folate polyglutamylation	100	1 of 1
66794	suberin monomers biosynthesis	100	2 of 2
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	vitamin B1 metabolism	92.31	12 of 13
66794	starch degradation	90	9 of 10
66794	propionate fermentation	90	9 of 10
66794	glutamate and glutamine metabolism	89.29	25 of 28
66794	valine metabolism	88.89	8 of 9
66794	molybdenum cofactor biosynthesis	88.89	8 of 9
66794	chorismate metabolism	88.89	8 of 9
66794	aspartate and asparagine metabolism	88.89	8 of 9
66794	isoleucine metabolism	87.5	7 of 8
66794	gluconeogenesis	87.5	7 of 8
66794	propanol degradation	85.71	6 of 7
66794	selenocysteine biosynthesis	83.33	5 of 6
66794	vitamin B12 metabolism	82.35	28 of 34
66794	vitamin K metabolism	80	4 of 5
66794	threonine metabolism	80	8 of 10
66794	factor 420 biosynthesis	80	4 of 5
66794	peptidoglycan biosynthesis	80	12 of 15
66794	cellulose degradation	80	4 of 5
66794	methylglyoxal degradation	80	4 of 5
66794	tetrahydrofolate metabolism	78.57	11 of 14
66794	photosynthesis	78.57	11 of 14
66794	CO2 fixation in Crenarchaeota	77.78	7 of 9
66794	NAD metabolism	77.78	14 of 18
66794	phenylalanine metabolism	76.92	10 of 13
66794	glycolysis	76.47	13 of 17
66794	acetate fermentation	75	3 of 4
66794	glycogen biosynthesis	75	3 of 4
66794	butanoate fermentation	75	3 of 4
66794	proline metabolism	72.73	8 of 11
66794	alanine metabolism	72.41	21 of 29
66794	ubiquinone biosynthesis	71.43	5 of 7
66794	citric acid cycle	71.43	10 of 14
66794	purine metabolism	70.21	66 of 94
66794	methionine metabolism	69.23	18 of 26
66794	leucine metabolism	69.23	9 of 13
66794	methane metabolism	66.67	2 of 3
66794	acetoin degradation	66.67	2 of 3
66794	cyanate degradation	66.67	2 of 3
66794	octane oxidation	66.67	2 of 3
66794	formaldehyde oxidation	66.67	2 of 3
66794	serine metabolism	66.67	6 of 9
66794	pyrimidine metabolism	66.67	30 of 45
66794	isoprenoid biosynthesis	65.38	17 of 26
66794	heme metabolism	64.29	9 of 14
66794	ketogluconate metabolism	62.5	5 of 8
66794	dTDPLrhamnose biosynthesis	62.5	5 of 8
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	62.5	5 of 8
66794	histidine metabolism	62.07	18 of 29
66794	urea cycle	61.54	8 of 13
66794	lipid metabolism	61.29	19 of 31
66794	cysteine metabolism	61.11	11 of 18
66794	flavin biosynthesis	60	9 of 15
66794	coenzyme M biosynthesis	60	6 of 10
66794	glycine betaine biosynthesis	60	3 of 5
66794	oxidative phosphorylation	59.34	54 of 91
66794	d-mannose degradation	55.56	5 of 9
66794	nitrate assimilation	55.56	5 of 9
66794	arginine metabolism	54.17	13 of 24
66794	non-pathway related	52.63	20 of 38
66794	biotin biosynthesis	50	2 of 4
66794	CMP-KDO biosynthesis	50	2 of 4
66794	aminopropanol phosphate biosynthesis	50	1 of 2
66794	pantothenate biosynthesis	50	3 of 6
66794	ethanol fermentation	50	1 of 2
66794	adipate degradation	50	1 of 2
66794	glycine metabolism	50	5 of 10
66794	degradation of sugar alcohols	50	8 of 16
66794	degradation of aromatic, nitrogen containing compounds	50	6 of 12
66794	kanosamine biosynthesis II	50	1 of 2
66794	lactate fermentation	50	2 of 4
66794	toluene degradation	50	2 of 4
66794	tryptophan metabolism	50	19 of 38
66794	lipid A biosynthesis	44.44	4 of 9
66794	degradation of hexoses	44.44	8 of 18
66794	polyamine pathway	43.48	10 of 23
66794	tyrosine metabolism	42.86	6 of 14
66794	cardiolipin biosynthesis	42.86	3 of 7
66794	lysine metabolism	40.48	17 of 42
66794	phenylacetate degradation (aerobic)	40	2 of 5
66794	creatinine degradation	40	2 of 5
66794	4-hydroxyphenylacetate degradation	40	4 of 10
66794	3-chlorocatechol degradation	40	2 of 5
66794	metabolism of amino sugars and derivatives	40	2 of 5
66794	D-cycloserine biosynthesis	40	2 of 5
66794	Entner Doudoroff pathway	40	4 of 10
66794	arachidonate biosynthesis	40	2 of 5
66794	myo-inositol biosynthesis	40	4 of 10
66794	phosphatidylethanolamine bioynthesis	38.46	5 of 13
66794	pentose phosphate pathway	36.36	4 of 11
66794	metabolism of disaccharids	36.36	4 of 11
66794	glutathione metabolism	35.71	5 of 14
66794	phenol degradation	35	7 of 20
66794	IAA biosynthesis	33.33	1 of 3
66794	acetyl CoA biosynthesis	33.33	1 of 3
66794	sulfoquinovose degradation	33.33	1 of 3
66794	enterobactin biosynthesis	33.33	1 of 3
66794	(5R)-carbapenem carboxylate biosynthesis	33.33	1 of 3
66794	glycolate and glyoxylate degradation	33.33	2 of 6
66794	degradation of pentoses	32.14	9 of 28
66794	sulfate reduction	30.77	4 of 13
66794	benzoyl-CoA degradation	28.57	2 of 7
66794	d-xylose degradation	27.27	3 of 11
66794	dolichyl-diphosphooligosaccharide biosynthesis	27.27	3 of 11
66794	vitamin B6 metabolism	27.27	3 of 11
66794	cyclohexanol degradation	25	1 of 4
66794	methanogenesis from CO2	25	3 of 12
66794	vitamin E metabolism	25	1 of 4
66794	ascorbate metabolism	22.73	5 of 22
66794	4-hydroxymandelate degradation	22.22	2 of 9

Isolation source categories

Isolation

Taxonmaps

69479

Global distribution of 16S sequence X91170 (>99% sequence identity) for Pelotomaculum schinkii subclade from Microbeatlas

Aquatic Samples	6
Soil Samples	2
Animal Samples	18
Plant Samples
Total Samples	26

Risk assessment

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	NCBI tax ID	Score
66792	ASM436920v1 assembly for Pelotomaculum schinkii HH	contig	GCA_004369205	78350.3	78350	75.89

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
5753	Pelotomaculum schinkii 16S rRNA gene, type strain HHT	X91169	1361		78350
5753	Pelotomaculum schinkii 16S rRNA gene, type strain HHT	X91170	1362		78350

Predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	The first true obligately syntrophic propionate-oxidizing bacterium, Pelotomaculum schinkii sp. nov., co-cultured with Methanospirillum hungatei, and emended description of the genus Pelotomaculum.	de Bok FAM, Harmsen HJM, Plugge CM, de Vries MC, Akkermans ADL, de Vos WM, Stams AJM	Int J Syst Evol Microbiol	10.1099/ijs.0.02880-0	2005