Pelotomaculum thermopropionicum (DSM 13744, JCM 10971, SI)

Name: Pelotomaculum thermopropionicum (DSM 13744, JCM 10971, SI)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 11833

Type strain

Strain Designation SI

Variant Co-culture with BacDive ID 11834

Culture col. no. DSM 13744 JCM 10971 SI

NCBI tax ID(s) 110500

Special Collections DSMZ JCM

History <- H. Imachi, Nagaoka Univ. Technology, Japan; SI <- H. Imachi {1999} H. Imachi SI.

Links

Pelotomaculum thermopropionicum SI is an anaerobe, thermophilic prokaryote that was isolated from granular sludge in a thermophilic upflow anaerobic sludge blanket reactor.

anaerobe thermophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 100857

DSM 13744,JCM 10971,DSM 13752

@ref 20215

Last LPSN update 2026-02-09 11:20:40

Domain Bacteria

Phylum Bacillota

Class Clostridia

Order Eubacteriales

Family Peptococcaceae

Genus Pelotomaculum

Species Pelotomaculum thermopropionicum

Full scientific name Pelotomaculum thermopropionicum Imachi et al. 2002

BacDive ID	Other strains from **Pelotomaculum thermopropionicum** (1)	Type strain
11834	P. thermopropionicum SI, DSM 13752, JCM 10971

Morphology

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
5142	PELOTOMACULUM MEDIUM (DSMZ Medium 960)		Medium recipe at MediaDive	Name: PELOTOMACULUM MEDIUM (DSMZ Medium 960) Composition: Na-pyruvate 2.19561 g/l Na2CO3 1.49701 g/l NH4Cl 0.538922 g/l Na2S x 9 H2O 0.299401 g/l L-Cysteine HCl x H2O 0.299401 g/l MgCl2 x 6 H2O 0.199601 g/l KH2PO4 0.139721 g/l Yeast extract 0.0998004 g/l Nitrilotriacetic acid 0.0127745 g/l NaCl 0.000998004 g/l FeCl2 x 4 H2O 0.000998004 g/l Sodium resazurin 0.000499002 g/l ZnCl2 9.98004e-05 g/l Pyridoxine hydrochloride 9.98004e-05 g/l CaCl2 x 2 H2O 9.98004e-05 g/l NiCl2 x 6 H2O 9.98004e-05 g/l MnCl2 x 4 H2O 9.98004e-05 g/l Calcium D-(+)-pantothenate 4.99002e-05 g/l Nicotinic acid 4.99002e-05 g/l Riboflavin 4.99002e-05 g/l p-Aminobenzoic acid 4.99002e-05 g/l (DL)-alpha-Lipoic acid 4.99002e-05 g/l Thiamine HCl 4.99002e-05 g/l Na2WO4 x 2 H2O 3.99202e-05 g/l CoCl2 x 6 H2O 2.99401e-05 g/l Na2SeO3 x 5 H2O 2.99401e-05 g/l Na2MoO4 x 2 H2O 2.99401e-05 g/l CuCl2 1.99601e-05 g/l Biotin 1.99601e-05 g/l Folic acid 1.99601e-05 g/l H3BO3 9.98004e-06 g/l Vitamin B12 9.98004e-07 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
5142	positive	growth	55	thermophilic
67770	positive	growth	55	thermophilic

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
5142	anaerobe
125439	anaerobe	99.7

Observation

67770

Observationquinones: MK-7, MK-7(H₄)

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Bioreactor	-
#Engineered	#Waste	#Activated sludge
#Condition	#Anoxic (anaerobic)	-

Isolation

@ref	Sample type	Country	Country ISO 3 Code	Continent
5142	granular sludge in a thermophilic upflow anaerobic sludge blanket reactor	Japan	JPN	Asia
67770	Methanogenic granular sludge in a thermophilic upflow anaerobic sludge blanket reactor

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
5142	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM1056v1 assembly for Pelotomaculum thermopropionicum SI	complete	GCA_000010565	370438.4	640427128	370438	70.77

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
5142	Pelotomaculum thermopropionicum SI gene for 16S rRNA	AB035723	1532		370438

GC content

@ref	GC-content (mol%)	Method
5142	52.8
67770	52.8	high performance liquid chromatography (HPLC)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Pelotomaculum thermopropionicum SI
PATRIC: 370438.4

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	82.20	no
125439	motility	BacteriaNetⓘ	yes	63.30	no
125439	gram_stain	BacteriaNetⓘ	positive	62.20	no
125439	oxygen_tolerance	BacteriaNetⓘ	anaerobe	99.70	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Pelotomaculum thermopropionicum SI SI
NCBI: GCA_000010565.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	57.31	no
125438	anaerobic	anaerobicⓘ	yes	86.15	yes
125438	aerobic	aerobicⓘ	no	92.32	no
125438	spore-forming	spore-formingⓘ	yes	77.41	no
125438	thermophilic	thermophileⓘ	yes	56.61	no
125438	flagellated	motile2+ⓘ	yes	65.41	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Enzymology	Membrane Potential-requiring Succinate Dehydrogenase Constitutes the Key to Propionate Oxidation and Is Unique to Syntrophic Propionate-oxidizing Bacteria.	Kosaka T, Tsushima Y, Shiota Y, Ishiguchi T, Matsushita K, Matsutani M, Yamada M.	Microbes Environ	10.1264/jsme2.me22111	2023
	Concerted Metabolic Shifts Give New Insights Into the Syntrophic Mechanism Between Propionate-Fermenting Pelotomaculum thermopropionicum and Hydrogenotrophic Methanocella conradii.	Liu P, Lu Y.	Front Microbiol	10.3389/fmicb.2018.01551	2018
Metabolism	Coaggregation facilitates interspecies hydrogen transfer between Pelotomaculum thermopropionicum and Methanothermobacter thermautotrophicus.	Ishii S, Kosaka T, Hori K, Hotta Y, Watanabe K.	Appl Environ Microbiol	10.1128/aem.71.12.7838-7845.2005	2005
	Identification and characterization of a novel fumarase gene by metagenome expression cloning from marine microorganisms.	Jiang C, Wu LL, Zhao GC, Shen PH, Jin K, Hao ZY, Li SX, Ma GF, Luo FF, Hu GQ, Kang WL, Qin XM, Bi YL, Tang XL, Wu B.	Microb Cell Fact	10.1186/1475-2859-9-91	2010
Enzymology	Molecular cloning, heterologous expression, and enzymatic characterization of lysoplasmalogen-specific phospholipase D from Thermocrispum sp.	Matsumoto Y, Kashiwabara N, Oyama T, Murayama K, Matsumoto H, Sakasegawa SI, Sugimori D.	FEBS Open Bio	10.1002/2211-5463.12131	2016
Metabolism	Reconstruction and regulation of the central catabolic pathway in the thermophilic propionate-oxidizing syntroph Pelotomaculum thermopropionicum.	Kosaka T, Uchiyama T, Ishii S, Enoki M, Imachi H, Kamagata Y, Ohashi A, Harada H, Ikenaga H, Watanabe K.	J Bacteriol	10.1128/jb.188.1.202-210.2006	2006
Metabolism	Predicted group II intron lineages E and F comprise catalytically active ribozymes.	Nagy V, Pirakitikulr N, Zhou KI, Chillon I, Luo J, Pyle AM.	RNA	10.1261/rna.039123.113	2013
Metabolism	Identification and isolation of anaerobic, syntrophic phthalate isomer-degrading microbes from methanogenic sludges treating wastewater from terephthalate manufacturing.	Qiu YL, Sekiguchi Y, Imachi H, Kamagata Y, Tseng IC, Cheng SS, Ohashi A, Harada H.	Appl Environ Microbiol	10.1128/aem.70.3.1617-1626.2004	2004
Metabolism	In vitro synthesis of polyhydroxyalkanoates using thermostable acetyl-CoA synthetase, CoA transferase, and PHA synthase from thermotorelant bacteria.	Tajima K, Han X, Hashimoto Y, Satoh Y, Satoh T, Taguchi S	J Biosci Bioeng	10.1016/j.jbiosc.2016.06.001	2016
Phylogeny	Pelotomaculum thermopropionicum gen. nov., sp. nov., an anaerobic, thermophilic, syntrophic propionate-oxidizing bacterium.	Imachi H, Sekiguchi Y, Kamagata Y, Hanada S, Ohashi A, Harada H	Int J Syst Evol Microbiol	10.1099/00207713-52-5-1729	2002

References

#5142	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 13744
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Pelotomaculum thermopropionicum (DSM 13744, JCM 10971, SI)

Name and taxonomic classification

Morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Observation

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000010565

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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