Mannheimia varigena (DSM 19166, CCUG 38462, CIP 106064)

Name: Mannheimia varigena (DSM 19166, CCUG 38462, CIP 106064)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 11725

Type strain

Strain Designation 177

Culture col. no. DSM 19166 CCUG 38462 CIP 106064 177

NCBI tax ID(s) 85404

Special Collections DSMZ CCUG CIP

Links

Mannheimia varigena 177 is an obligate aerobe, mesophilic, Gram-negative animal pathogen that was isolated from cattle.

Gram-negative rod-shaped obligate aerobe mesophilic animal pathogen genome sequence 16S sequence

Name and taxonomic classification

SI-ID 69232

CIP 106064,CCUG 38462,DSM 19166

@ref 20215

Last LPSN update 2025-12-16 09:49:40

Domain Pseudomonadati

Phylum Pseudomonadota

Class Gammaproteobacteria

Order Pasteurellales

Family Pasteurellaceae

Genus Mannheimia

Species Mannheimia varigena

Full scientific name Mannheimia varigena Angen et al. 1999

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Confidence
123802	negative	rod-shaped
125438			92.988
125438	negative		97.5

Colony morphology

123802

Hemolysis ability1

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
7946	COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)	Medium recipe at MediaDive	Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
7946	BHI MEDIUM (DSMZ Medium 215)	Medium recipe at MediaDive	Name: BHI MEDIUM (DSMZ Medium 215) Composition: Brain heart infusion 37.0 g/l Distilled water
40150	MEDIUM 118 - for Pasteurella, Actinobacillus and Psychrobacter frigidicola		Distilled water make up to (1000.000 ml);Yeast extract (1.000 g);Tryptocasein soy agar (40.000 g)
123802	CIP Medium 118-b	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
7946	positive	growth	37	mesophilic
40150	positive	growth	37	mesophilic
123802	positive	growth	30-41
123802	negative	growth	15

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
123802	obligate aerobe
125439	obligate aerobe	95.3

Spore formation

@ref	Spore formation	Confidence
125438		91.019
125439		94.8

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68371	27613 ChEBI	amygdalin	-	builds acid from	from API 50CH acid
68371	18305 ChEBI	arbutin	-	builds acid from	from API 50CH acid
68371	17057 ChEBI	cellobiose	-	builds acid from	from API 50CH acid
123802	16947 ChEBI	citrate	-	carbon source
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
68371	15824 ChEBI	D-fructose	+	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	12936 ChEBI	D-galactose	+	builds acid from	from API 50CH acid
68377	17634 ChEBI	D-glucose	+	builds acid from	from API NH
68371	17634 ChEBI	D-glucose	+	builds acid from	from API 50CH acid
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68371	16899 ChEBI	D-mannitol	+	builds acid from	from API 50CH acid
68371	16024 ChEBI	D-mannose	-	builds acid from	from API 50CH acid
68371	16988 ChEBI	D-ribose	+	builds acid from	from API 50CH acid
68371	17924 ChEBI	D-sorbitol	-	builds acid from	from API 50CH acid
68371	16443 ChEBI	D-tagatose	-	builds acid from	from API 50CH acid
68371	65327 ChEBI	D-xylose	+	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
123802	4853 ChEBI	esculin	-	hydrolysis
68371	4853 ChEBI	esculin	-	builds acid from	from API 50CH acid
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
68371	28066 ChEBI	gentiobiose	-	builds acid from	from API 50CH acid
68371	24265 ChEBI	gluconate	+	builds acid from	from API 50CH acid
123802	17234 ChEBI	glucose	+	fermentation
123802	17234 ChEBI	glucose	+	degradation
68371	17754 ChEBI	glycerol	+	builds acid from	from API 50CH acid
68371	28087 ChEBI	glycogen	-	builds acid from	from API 50CH acid
123802	606565 ChEBI	hippurate	+	hydrolysis
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
68371	30849 ChEBI	L-arabinose	+	builds acid from	from API 50CH acid
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	+	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	+	builds acid from	from API 50CH acid
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
123802	17716 ChEBI	lactose	-	fermentation
68371	17716 ChEBI	lactose	-	builds acid from	from API 50CH acid
68371	17306 ChEBI	maltose	-	builds acid from	from API 50CH acid
68371	6731 ChEBI	melezitose	-	builds acid from	from API 50CH acid
68371	28053 ChEBI	melibiose	-	builds acid from	from API 50CH acid
68371	320061 ChEBI	methyl alpha-D-glucopyranoside	-	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
68371	17268 ChEBI	myo-inositol	+	builds acid from	from API 50CH acid
68371	59640 ChEBI	N-acetylglucosamine	+	builds acid from	from API 50CH acid
123802	17632 ChEBI	nitrate	+	reduction
123802	16301 ChEBI	nitrite	-	reduction
68377	18257 ChEBI	ornithine	-	degradation	from API NH
68371		Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371		Potassium 5-ketogluconate	-	builds acid from	from API 50CH acid
68371	16634 ChEBI	raffinose	-	builds acid from	from API 50CH acid
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
68371	17814 ChEBI	salicin	-	builds acid from	from API 50CH acid
123802	132112 ChEBI	sodium thiosulfate	-	builds gas from
68371	28017 ChEBI	starch	-	builds acid from	from API 50CH acid
68371	17992 ChEBI	sucrose	+	builds acid from	from API 50CH acid
68371	27082 ChEBI	trehalose	-	builds acid from	from API 50CH acid
68377	27897 ChEBI	tryptophan	-	energy source	from API NH
68371	32528 ChEBI	turanose	-	builds acid from	from API 50CH acid
68377	16199 ChEBI	urea	-	hydrolysis	from API NH
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

Antibiotic resistance

@ref	Metabolite	Is sensitive	Is resistant
123802	0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68377	35581 ChEBI	indole		from API NH
123802	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test	Voges-proskauer-test	Methylred-test
123802	15688 ChEBI	acetoin		-
123802	17234 ChEBI	glucose			+
68377	35581 ChEBI	indole	-			from API NH

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
123802	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68377	alkaline phosphatase	+	3.1.3.1	from API NH
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	-	3.2.1.23	from API zym
123802	beta-galactosidase	+	3.2.1.23
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
123802	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	-		from API zym
68382	esterase lipase (C 8)	-		from API zym
68377	gamma-glutamyltransferase	-	2.3.2.2	from API NH
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
123802	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
123802	ornithine decarboxylase	+	4.1.1.17
68377	ornithine decarboxylase	-	4.1.1.17	from API NH
123802	oxidase	+
123802	phenylalanine ammonia-lyase	-	4.3.1.24
68377	proline-arylamidase	-	3.4.11.5	from API NH
68382	trypsin	-	3.4.21.4	from API zym
123802	tryptophan deaminase	-
68377	tryptophan deaminase	-	4.1.99.1	from API NH
123802	urease	-	3.5.1.5
68377	urease	-	3.5.1.5	from API NH
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
123802	-	+	-	-	-	+	-	-	-	-	+	+	-	-	-	-	-	-	-	-

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
123802	not determinedn.d.	+	-	-	+	+	+	-	-	-	+	+	+	-	-	+	-	+	+	-	-	-	+	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	+	-	-

API NH

@ref	PEN	GLU	FRU	MAL	SAC	ODC	URE	LIP	PAL	beta GAL	ProA	GGT	IND
7946	+	+	-	+	-	-	-	+	+	-	-	-	-
7946	-	+	+	-	+	-	-	-	+	+	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Mammals	#Bovinae (Cow, Cattle)

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
7946	cattle		Germany	DEU	Europe
54269	Bovine pneumonia	West	Germany	DEU	Europe
123802	Animal, Bovine, pneumonia	West	Germany	DEU	Europe

Taxonmaps

69479

Global distribution of 16S sequence AF053893 (>99% sequence identity) for Mannheimia varigena subclade from Microbeatlas

Aquatic Samples	55
Soil Samples	19
Animal Samples	1694
Plant Samples	3
Total Samples	1771

Interaction and safety

Risk assessment

@ref	Pathogenicity animal	Biosafety level	Biosafety level comment
7946		2	Risk group (German classification) According to TRBA 466
123802		1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM1337723v1 assembly for Mannheimia varigena 177	complete	GCA_013377235	85404.47	8113805292	85404	94.51

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
7946	Mannheimia varigena strain 177; CCUG 38462 16S ribosomal RNA gene, partial sequence	AF053893	1504		85404

GC content

7946

GC-content (mol%)41.7

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Mannheimia varigena 177
NCBI: GCA_013377235

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	94.80	no
125439	motility	BacteriaNetⓘ	no	64.30	no
125439	gram_stain	BacteriaNetⓘ	negative	70.40	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	95.30	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Mannheimia varigena 177
NCBI: GCA_013377235.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	97.50	no
125438	anaerobic	anaerobicⓘ	no	91.58	no
125438	aerobic	aerobicⓘ	no	62.98	yes
125438	spore-forming	spore-formingⓘ	no	91.02	no
125438	thermophilic	thermophileⓘ	no	98.50	no
125438	flagellated	motile2+ⓘ	no	92.99	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Enzymology	Mycoplasma detection by triplex real-time PCR in bronchoalveolar lavage fluid from bovine respiratory disease complex cases.	Cornelissen JB, de Bree FM, van der Wal FJ, Kooi EA, Koene MG, Bossers A, Smid B, Antonis AF, Wisselink HJ.	BMC Vet Res	10.1186/s12917-017-1023-6	2017
	Evaluation of a multiplex real-time PCR for detection of four bacterial agents commonly associated with bovine respiratory disease in bronchoalveolar lavage fluid.	Wisselink HJ, Cornelissen JBWJ, van der Wal FJ, Kooi EA, Koene MG, Bossers A, Smid B, de Bree FM, Antonis AFG.	BMC Vet Res	10.1186/s12917-017-1141-1	2017
	A Sensitive and Accurate Recombinase Polymerase Amplification Assay for Detection of the Primary Bacterial Pathogens Causing Bovine Respiratory Disease.	Conrad CC, Daher RK, Stanford K, Amoako KK, Boissinot M, Bergeron MG, Alexander T, Cook S, Ralston B, Zaheer R, Niu YD, McAllister T.	Front Vet Sci	10.3389/fvets.2020.00208	2020
Enzymology	Rapid and accurate identification of human isolates of Pasteurella and related species by sequencing the sodA gene.	Gautier AL, Dubois D, Escande F, Avril JL, Trieu-Cuot P, Gaillot O.	J Clin Microbiol	10.1128/jcm.43.5.2307-2314.2005	2005
Phylogeny	Taxonomic relationships of the [Pasteurella] haemolytica complex as evaluated by DNA-DNA hybridizations and 16S rRNA sequencing with proposal of Mannheimia haemolytica gen. nov., comb. nov., Mannheimia granulomatis comb. nov., Mannheimia glucosida sp. nov., Mannheimia ruminalis sp. nov. and Mannheimia varigena sp. nov.	Angen O, Mutters R, Caugant DA, Olsen JE, Bisgaard M	Int J Syst Bacteriol	10.1099/00207713-49-1-67	1999

References

#7946	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 19166
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#40150	; Curators of the CIP;
#54269	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 38462
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#68371	Automatically annotated from API 50CH acid .
#68377	Automatically annotated from API NH .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#123802	Collection of Institut Pasteur ; Curators of the CIP; CIP 106064
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Mannheimia varigena (DSM 19166, CCUG 38462, CIP 106064)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Metabolite utilization

Antibiotic resistance

Metabolite production

Metabolite tests

Enzymes

API zym

API 50CHac

API NH

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_013377235

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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