Name: Mannheimia varigena 177
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 11725

Type strain:

Species: Mannheimia varigena

Strain Designation: 177

Culture col. no.: DSM 19166, CCUG 38462, CIP 106064

Strain history: CIP <- 1999, CCUG <- Ö. Angen, SVS, Copenhagen, Denmark: strain 177

NCBI tax ID(s): 85404 (species)

SI-ID 69232

CIP 106064, CCUG 38462, DSM 19166

Section

Mannheimia varigena 177 is an obligate aerobe, mesophilic, Gram-negative animal pathogen that was isolated from cattle.

Gram-negative
rod-shaped
animal pathogen
mesophilic
obligate aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Pasteurellales*
	Family	*Pasteurellaceae*
	Genus	*Mannheimia*
	Species	**Mannheimia varigena**
	Full Scientific Name (LPSN)	Mannheimia varigena Angen et al. 1999

Information on morphological and physiological properties Morphology

[Ref.: #123802]	Gram stain	negative

[Ref.: #123802]	Cell shape	rod-shaped

[Ref.: #123802]	Motility	no

[Ref.: #123802]

Hemolysis ability

Information on culture and growth conditions Culture and growth conditions

[Ref.: #7946]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #7946]

Culture medium growth

[Ref.: #7946]

Culture medium link

Medium recipe at MediaDive

[Ref.: #7946]

Culture medium composition

expand / minimize

[Ref.: #7946]

Culture medium

BHI MEDIUM (DSMZ Medium 215)

[Ref.: #7946]

Culture medium growth

[Ref.: #7946]

Culture medium link

Medium recipe at MediaDive

[Ref.: #7946]

Culture medium composition

expand / minimize

[Ref.: #40150]

Culture medium

MEDIUM 118 - for Pasteurella, Actinobacillus and Psychrobacter frigidicola

[Ref.: #40150]

Culture medium growth

[Ref.: #40150]

Culture medium composition

expand / minimize

[Ref.: #123802]

Culture medium

CIP Medium 118-b

[Ref.: #123802]

Culture medium growth

[Ref.: #123802]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #7946]		growth	37 °C
[Ref.: #40150]		growth	37 °C
[Ref.: #123802]		growth	30-41 °C
[Ref.: #123802]	no	growth	15 °C

[Ref.: #7946]	Temperature range	mesophilic
[Ref.: #40150]	Temperature range	mesophilic
[Ref.: #123802]	Temperature range	psychrophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #123802]

Oxygen tolerance

obligate aerobe

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68371]	amygdalin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	arbutin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	cellobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #123802]	citrate ChEBI	-	carbon source
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fructose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-galactose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-glucose ChEBI	+	builds acid from	* from API 50CH acid, API NH
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-ribose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-xylose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #123802]	esculin ChEBI	-	hydrolysis
[Ref.: #68371]	esculin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gentiobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gluconate ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #123802]	glucose ChEBI	+	fermentation
[Ref.: #123802]	glucose ChEBI	+	degradation
[Ref.: #68371]	glycerol ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycogen ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #123802]	hippurate ChEBI	+	hydrolysis
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabinose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #123802]	lactose ChEBI	-	fermentation
[Ref.: #68371]	lactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	maltose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melezitose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melibiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	N-acetylglucosamine ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #123802]	nitrate ChEBI	+	reduction
[Ref.: #123802]	nitrite ChEBI	-	reduction
[Ref.: #68377]	ornithine ChEBI	-	degradation	* from API NH
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	raffinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	salicin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #123802]	sodium thiosulfate ChEBI	-	builds gas from
[Ref.: #68371]	starch ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	sucrose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	trehalose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68377]	tryptophan ChEBI	-	energy source	* from API NH
[Ref.: #68371]	turanose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68377]	urea ChEBI	-	hydrolysis	* from API NH
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

	Antibiotica	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #123802]		0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)	no	yes

	Metabolite production	Metabolite	Production
[Ref.: #123802]		indole ChEBI	no
[Ref.: #68377]		indole ChEBI	no	* from API NH

	Metabolite	Voges-Proskauer-test	Methylred-test	Indole test
[Ref.: #123802]	acetoin ChEBI	-
[Ref.: #123802]	glucose ChEBI		+
[Ref.: #68377]	indole ChEBI			-	* from API NH

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #123802]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68377]	alkaline phosphatase	+	3.1.3.1	* from API NH, API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #123802]	beta-galactosidase	+	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #123802]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	-		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #68377]	gamma-glutamyltransferase	-	2.3.2.2	* from API NH
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #123802]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #123802]	ornithine decarboxylase	+	4.1.1.17
[Ref.: #68377]	ornithine decarboxylase	-	4.1.1.17	* from API NH
[Ref.: #123802]	oxidase	+
[Ref.: #123802]	phenylalanine ammonia-lyase	-	4.3.1.24
[Ref.: #68377]	proline-arylamidase	-	3.4.11.5	* from API NH
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #123802]	tryptophan deaminase	-
[Ref.: #68377]	tryptophan deaminase	-	4.1.99.1	* from API NH
[Ref.: #123802]	urease	-	3.5.1.5
[Ref.: #68377]	urease	-	3.5.1.5	* from API NH
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #123802]	10387	not determinedn.d.	+	-	-	+	+	+	-	-	-	+	+	+	-	-	+	-	+	+	-	-	-	+	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	+	-	-

API® NH:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Penicillinase productionPEN	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from maltoseMAL	Acid from sucroseSAC	Ornithine decarboxylaseODC	Urease/urea hydrolysisURE	LipaseLIP	Alkaline phosphatasePAL	beta-Galactosidasebeta GAL	Proline arylamidaseProA	gamma-Glutamyl transferaseGGT	Indole productionIND
[Ref.: #7946]	6119	+	+	-	+	-	-	-	+	+	-	-	-	-
[Ref.: #7946]	5753	-	+	+	-	+	-	-	-	+	+	-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #123802]	9223	-	+	-	-	-	+	-	-	-	-	+	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #7946]	Sample type/isolated from	cattle
[Ref.: #7946]	Country	Germany
[Ref.: #7946]	Country ISO 3 Code	DEU
[Ref.: #7946]	Continent	Europe

[Ref.: #54269]	Sample type/isolated from	Bovine pneumonia
[Ref.: #54269]	Geographic location (country and/or sea, region)	West
[Ref.: #54269]	Country	Germany
[Ref.: #54269]	Country ISO 3 Code	DEU
[Ref.: #54269]	Continent	Europe

[Ref.: #123802]	Sample type/isolated from	Animal, Bovine, pneumonia
[Ref.: #123802]	Geographic location (country and/or sea, region)	West
[Ref.: #123802]	Country	Germany
[Ref.: #123802]	Country ISO 3 Code	DEU
[Ref.: #123802]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Host

#Mammals

#Bovinae (Cow, Cattle)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AF053893 (>99% sequence identity) for Mannheimia varigena subclade from Microbeatlas

Aquatic Samples	55
Soil Samples	19
Animal Samples	1694
Plant Samples	3
Total Samples	1771

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #7946]	Pathogenicity (animal)	yes
[Ref.: #7946]	Biosafety level	2	Risk group (German classification) According to TRBA 466
[Ref.: #123802]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #7946]	Mannheimia varigena strain 177; CCUG 38462 16S ribosomal RNA gene, partial sequence	AF053893	1504	ENA	85404 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Mannheimia varigena 177	GCA_013377235	complete	GenBank	85404 tax ID	*

[Ref.: #7946]

GC-content

41.7 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Mannheimia varigena 177 NCBI: GCA_013377235.1
[Ref.: #69480]	motile	no	89.358	no
[Ref.: #69480]	flagellated	no	95.672	no
[Ref.: #69480]	gram-positive	no	98.07	no
[Ref.: #69480]	anaerobic	no	98.1	no
[Ref.: #69480]	aerobic	no	90.506	no
[Ref.: #69480]	halophile	no	59.68	no
[Ref.: #69480]	spore-forming	no	98.759	no
[Ref.: #69480]	thermophile	no	98.198	yes
[Ref.: #69480]	glucose-util	no	58.797	no
[Ref.: #69480]	glucose-ferment	no	56.4	no

Availability in culture collections External links

[Ref.: #7946]

Culture collection no.

DSM 19166, CCUG 38462, CIP 106064

[Ref.: #80944]

SI-ID 69232

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Taxonomic relationships of the [Pasteurella] haemolytica complex as evaluated by DNA-DNA hybridizations and 16S rRNA sequencing with proposal of Mannheimia haemolytica gen. nov., comb. nov., Mannheimia granulomatis comb. nov., Mannheimia glucosida sp. nov., Mannheimia ruminalis sp. nov. and Mannheimia varigena sp. nov.	Angen O, Mutters R, Caugant DA, Olsen JE, Bisgaard M	Int J Syst Bacteriol	10.1099/00207713-49-1-67	1999	*

References

#7946

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 19166

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#40150 ; Curators of the CIP;
#54269 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 38462

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68371

Automatically annotated from API 50CH acid .

#68377

Automatically annotated from API NH .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#80944

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID69232.1 )

#123802 Collection of Institut Pasteur ; Curators of the CIP; CIP 106064
* These data were automatically processed and therefore are not curated

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