Name: Actinobacillus scotiae M2000/95/1
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 11686

Type strain:

Species: Actinobacillus scotiae

Strain Designation: M2000/95/1

Culture col. no.: DSM 22205, CCUG 46379, CIP 105697, NCTC 12922

Strain history: CIP <- 1998, G. Foster, SAC Vet. Services, Inverness, UK: strain M2000/95/1

NCBI tax ID(s): 182182 (species)

SI-ID 44155

ATCC 700820, NCTC 12922, CIP 105697, CCUG 46379, NCIMB 13548, DSM 22205

Section

Actinobacillus scotiae M2000/95/1 is a microaerophile, mesophilic, Gram-negative animal pathogen that was isolated from viscera of porpoises .

Gram-negative
rod-shaped
animal pathogen
mesophilic
microaerophile
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Pasteurellales*
	Family	*Pasteurellaceae*
	Genus	*Actinobacillus*
	Species	**Actinobacillus scotiae**
	Full Scientific Name (LPSN)	Actinobacillus scotiae Foster et al. 1998

Information on morphological and physiological properties Morphology

[Ref.: #122527]	Gram stain	negative

[Ref.: #122527]	Cell shape	rod-shaped

[Ref.: #122527]	Motility	no

[Ref.: #16201]	Type of hemolysis	gamma

[Ref.: #122527]	Hemolysis ability	1

Information on culture and growth conditions Culture and growth conditions

[Ref.: #16201]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #16201]

Culture medium growth

[Ref.: #16201]

Culture medium link

Medium recipe at MediaDive

[Ref.: #16201]

Culture medium composition

expand / minimize

[Ref.: #37219]

Culture medium

MEDIUM 45 - for Columbia agar with sheep blood

[Ref.: #37219]

Culture medium growth

[Ref.: #37219]

Culture medium composition

expand / minimize

[Ref.: #122527]

Culture medium

CIP Medium 45

[Ref.: #122527]

Culture medium growth

[Ref.: #122527]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #16201]	growth	37 °C
[Ref.: #37219]	growth	37 °C
[Ref.: #57177]	growth	37 °C
[Ref.: #122527]	growth	37 °C

[Ref.: #16201]	Temperature range	mesophilic
[Ref.: #37219]	Temperature range	mesophilic
[Ref.: #57177]	Temperature range	mesophilic
[Ref.: #122527]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #16201]	Oxygen tolerance	microaerophile
[Ref.: #57177]	Oxygen tolerance	microaerophile
[Ref.: #122527]	Oxygen tolerance	facultative anaerobe

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #122527]	esculin ChEBI	-	hydrolysis
[Ref.: #122527]	hippurate ChEBI	-	hydrolysis
[Ref.: #68377]	maltose ChEBI	-	builds acid from	* from API NH
[Ref.: #122527]	nitrate ChEBI	+	reduction
[Ref.: #122527]	nitrite ChEBI	-	reduction
[Ref.: #68377]	ornithine ChEBI	+	degradation	* from API NH
[Ref.: #68377]	tryptophan ChEBI	-	energy source	* from API NH
[Ref.: #68377]	urea ChEBI	+	hydrolysis	* from API NH

	Antibiotica	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #122527]		0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)	yes	no

	Metabolite production	Metabolite	Production
[Ref.: #122527]		indole ChEBI	no
[Ref.: #68377]		indole ChEBI	no	* from API NH

	Physiological tests	Metabolite	Voges-Proskauer-test	Indole test
[Ref.: #122527]		acetoin ChEBI	+
[Ref.: #68377]		indole ChEBI		-	* from API NH

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #122527]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #122527]	beta-galactosidase	+	3.2.1.23
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #16201]	catalase	-	1.11.1.6
[Ref.: #122527]	catalase	-	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #122527]	gamma-glutamyltransferase	+	2.3.2.2
[Ref.: #68377]	gamma-glutamyltransferase	-	2.3.2.2	* from API NH
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #122527]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #122527]	ornithine decarboxylase	+	4.1.1.17
[Ref.: #68377]	ornithine decarboxylase	+	4.1.1.17	* from API NH
[Ref.: #122527]	oxidase	-
[Ref.: #68377]	proline-arylamidase	-	3.4.11.5	* from API NH
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68377]	tryptophan deaminase	-	4.1.99.1	* from API NH
[Ref.: #122527]	urease	+	3.5.1.5
[Ref.: #68377]	urease	+	3.5.1.5	* from API NH
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® NH:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Penicillinase productionPEN	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from maltoseMAL	Acid from sucroseSAC	Ornithine decarboxylaseODC	Urease/urea hydrolysisURE	LipaseLIP	Alkaline phosphatasePAL	beta-Galactosidasebeta GAL	Proline arylamidaseProA	gamma-Glutamyl transferaseGGT	Indole productionIND
[Ref.: #16201]	6152	+	+/-	-	-	+	+	+	-	+	-	-	-	-
[Ref.: #16201]	5720	-	+	+/-	-	-	+	+	+	-	+	-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #122527]	8916	-	+	+	+	-	+	-	-	-	-	+	+	-	+	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #16201]	Sample type/isolated from	viscera of porpoises (Phocoena phocoena)
[Ref.: #16201]	Host species	Phocoena phocoena
[Ref.: #16201]	Geographic location (country and/or sea, region)	Scottish coastline
[Ref.: #16201]	Country	United Kingdom
[Ref.: #16201]	Country ISO 3 Code	GBR
[Ref.: #16201]	Continent	Europe

[Ref.: #57177]	Sample type/isolated from	Porpoise viscera
[Ref.: #57177]	Sampling date	1995
[Ref.: #57177]	Geographic location (country and/or sea, region)	North Sea

[Ref.: #122527]	Sample type/isolated from	Porpoise, viscera
[Ref.: #122527]	Geographic location (country and/or sea, region)	North Sea, Scotland
[Ref.: #122527]	Country	United Kingdom
[Ref.: #122527]	Country ISO 3 Code	GBR
[Ref.: #122527]	Continent	Europe
[Ref.: #122527]	Isolation date	1995
* marker position based on {}

Isolation sources categories

#Host	#Mammals	-
#Host Body-Site	#Organ	-

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #16201]	Pathogenicity (animal)	yes
[Ref.: #16201]	Biosafety level	2	Risk group (German classification) According to TRBA 466
[Ref.: #122527]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #16201]	Actinobacillus scotiae 16S rRNA gene, strain M2000/95/1	Y09653	1497	ENA	182182 tax ID

[Ref.: #16201]

GC-content

40.5 mol%

Availability in culture collections External links

[Ref.: #16201]

Culture collection no.

DSM 22205, CCUG 46379, CIP 105697, NCTC 12922

[Ref.: #80906]

SI-ID 44155

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Actinobacillus scotiae sp. nov., a new member of the family Pasteurellaceae Pohl (1979) 1981 isolated from porpoises (Phocoena phocoena).	Foster G, Ross HM, Patterson IA, Hutson RA, Collins MD	Int J Syst Bacteriol	10.1099/00207713-48-3-929	1998	*

References

#16201

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 22205

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#37219 ; Curators of the CIP;
#57177 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 46379

#68377

Automatically annotated from API NH .

#68382

Automatically annotated from API zym .

#80906

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID44155.1 )

#122527 Collection of Institut Pasteur ; Curators of the CIP; CIP 105697
* These data were automatically processed and therefore are not curated

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