Actinobacillus ureae (DSM 5568, ATCC 25976, NCTC 10219)

Name: Actinobacillus ureae (DSM 5568, ATCC 25976, NCTC 10219)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 11670

Type strain

Strain Designation 3520/59

Culture col. no. DSM 5568 ATCC 25976 NCTC 10219 CCUG 2139 3520/59 2 more

NCBI tax ID(s) 887324 723

Special Collections DSMZ CCUG CIP

Links

Actinobacillus ureae 3520/59 is a microaerophile, mesophilic, Gram-negative prokaryote that was isolated from nose swab.

Gram-negative rod-shaped microaerophile mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 38738

ATCC 25976,NCTC 10219,CCUG 2139,CCUG 37219,CCUG 37220,CCM 5774,DSM 5568,CCM 4689,CIP 62.8

@ref 20215

Last LPSN update 2025-12-16 09:49:39

Domain Pseudomonadati

Phylum Pseudomonadota

Class Gammaproteobacteria

Order Pasteurellales

Family Pasteurellaceae

Genus Actinobacillus

Species Actinobacillus ureae

Full scientific name Actinobacillus ureae (Jones 1962) Mutters et al. 1986

Synonyms (1)

Pasteurella ureae

BacDive ID	Other strains from **Actinobacillus ureae** (24)	Type strain
134798	A. ureae Martin, CIP 66.8
134914	A. ureae 745-86, CIP 102750
135114	A. ureae 612-86, CIP 102605
135839	A. ureae 595-85, CIP 101756
136083	A. ureae 687-86, CIP 102698
137004	A. ureae CIP 62.10, ATCC 29691, NCTC 10220
137494	A. ureae CIP 62.11, ATCC 29692, NCTC 10221
137760	A. ureae CIP 82.18
137788	A. ureae 915-85, CIP 101963
137891	A. ureae 38-86, CIP 102123
138627	A. ureae Névot, CIP 82.23
138640	A. ureae 415, 627-85, CIP 101808
138891	A. ureae CIP 62.12
141335	A. ureae CCUG 999
142640	A. ureae CCUG 13515
144745	A. ureae CCUG 26446
144746	A. ureae CCUG 26447
150500	A. ureae CCUG 41433
152319	A. ureae CCUG 46419
152408	A. ureae CCUG 46713
152804	A. ureae CCUG 47658
152805	A. ureae CCUG 47659
153136	A. ureae CCUG 48551
156773	A. ureae CCUG 65047

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Confidence
123738	negative	rod-shaped
125439	negative		97
125438			91.321
125438	negative		99

Colony morphology

123738

Hemolysis ability1

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
2224	COLUMBIA BLOOD AGAR (DSMZ Medium 429)	Medium recipe at MediaDive	Name: COLUMBIA BLOOD AGAR (DSMZ Medium 429) Composition: Horse blood 40.0 g/l Columbia agar base
37608	MEDIUM 6 - Columbia agar with 10 % horse blood		Distilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
123738	CIP Medium 6	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
2224	positive	growth	37	mesophilic
37608	positive	growth	37	mesophilic
44498	positive	growth	37	mesophilic
123738	positive	growth	25-37	mesophilic
123738	negative	growth	15
123738	negative	growth	41
123738	negative	growth	45

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
44498	microaerophile
125439	obligate aerobe	95.6

Antibiotic susceptibility testing

	Test 1
@ref	2224
Medium	M 693
Manual_annotation	1
	Inhibition zone diameter in mm
Penicillin G	34
Oxacillin	16-18
Ampicillin	34
Ticarcillin	40
Mezlocillin	36-38
Cefalotin	38
Cefazolin	32
Cefotaxime	44-46
Aztreonam	42
Imipenem	30
Tetracycline	28
Chloramphenicol	36-38
Gentamycin	14
Amikacin	16
Vancomycin	0
Erythromycin	24-26
Lincomycin	14-16
Ofloxacin	30
Norfloxacin	28
Colistin	14-16
Pipemidic acid	20-22
Nitrofurantoin	30
Bacitracin	12-14
Polymyxin b	16-18
Kanamycin	26
Neomycin	12
Doxycycline	26
Ceftriaxone	42
Clindamycin	22
Fosfomycin	24
Moxifloxacin	30
Linezolid	10-12
Nystatin	0
Quinupristin/dalfopristin	16-18
Teicoplanin	0
Piperacillin/tazobactam	38

Spore formation

@ref	Spore formation	Confidence
125439		98
125438		91.997

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68371	27613 ChEBI	amygdalin	-	builds acid from	from API 50CH acid
68371	18305 ChEBI	arbutin	-	builds acid from	from API 50CH acid
68371	17057 ChEBI	cellobiose	-	builds acid from	from API 50CH acid
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	12936 ChEBI	D-galactose	-	builds acid from	from API 50CH acid
68371	17634 ChEBI	D-glucose	+	builds acid from	from API 50CH acid
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68371	16899 ChEBI	D-mannitol	+	builds acid from	from API 50CH acid
68371	16024 ChEBI	D-mannose	-	builds acid from	from API 50CH acid
68371	17924 ChEBI	D-sorbitol	-	builds acid from	from API 50CH acid
68371	16443 ChEBI	D-tagatose	-	builds acid from	from API 50CH acid
68371	65327 ChEBI	D-xylose	-	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
123738	4853 ChEBI	esculin	-	hydrolysis
68371	4853 ChEBI	esculin	-	builds acid from	from API 50CH acid
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
68371	28066 ChEBI	gentiobiose	-	builds acid from	from API 50CH acid
68371	24265 ChEBI	gluconate	-	builds acid from	from API 50CH acid
68371	17754 ChEBI	glycerol	-	builds acid from	from API 50CH acid
68371	28087 ChEBI	glycogen	-	builds acid from	from API 50CH acid
123738	606565 ChEBI	hippurate	+	hydrolysis
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
68371	30849 ChEBI	L-arabinose	-	builds acid from	from API 50CH acid
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	-	builds acid from	from API 50CH acid
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
68371	17716 ChEBI	lactose	-	builds acid from	from API 50CH acid
68371	17306 ChEBI	maltose	-	builds acid from	from API 50CH acid
68371	6731 ChEBI	melezitose	-	builds acid from	from API 50CH acid
68371	28053 ChEBI	melibiose	-	builds acid from	from API 50CH acid
68371	320061 ChEBI	methyl alpha-D-glucopyranoside	-	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
68371	59640 ChEBI	N-acetylglucosamine	-	builds acid from	from API 50CH acid
123738	17632 ChEBI	nitrate	+	reduction
123738	16301 ChEBI	nitrite	-	reduction
68371		Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371		Potassium 5-ketogluconate	-	builds acid from	from API 50CH acid
68371	16634 ChEBI	raffinose	-	builds acid from	from API 50CH acid
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
68371	17814 ChEBI	salicin	-	builds acid from	from API 50CH acid
68371	28017 ChEBI	starch	-	builds acid from	from API 50CH acid
68371	27082 ChEBI	trehalose	-	builds acid from	from API 50CH acid
68371	32528 ChEBI	turanose	-	builds acid from	from API 50CH acid
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

Antibiotic resistance

@ref	Metabolite	Is antibiotic	Is sensitive	Is resistant
123738	0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)

Metabolite production

@ref	Chebi-ID	Metabolite	Production
123738	35581 ChEBI	indole

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
123738	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
123738	beta-galactosidase	-	3.2.1.23
68382	beta-galactosidase	-	3.2.1.23	from API zym
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
123738	catalase	-	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	-		from API zym
68382	esterase lipase (C 8)	-		from API zym
123738	gamma-glutamyltransferase	-	2.3.2.2
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
123738	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
123738	ornithine decarboxylase	-	4.1.1.17
123738	oxidase	+
68382	trypsin	-	3.4.21.4	from API zym
123738	tryptophan deaminase	-
123738	urease	+	3.5.1.5
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
123738	-	+	-	-	-	+	-	-	-	-	+	+	-	-	-	-	-	-	-	-

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
123738	not determinedn.d.	-	-	-	-	+/-	-	-	-	-	-	+	+/-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	+/-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Infection	#Patient	#Swab
#Host Body-Site	#Organ	#Nose

Isolation

@ref	Sample type	Sampling date	Country	Country ISO 3 Code	Continent	Isolation date
2224	nose swab
44498	Human nose,46-yr-old man living in rural disctrict	1959	Norway	NOR	Europe
123738	Nasal swab		Norway	NOR	Europe	1959

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
2224	2	Risk group (German classification) According to TRBA 466
123738	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM18825v1 assembly for Actinobacillus ureae ATCC 25976	scaffold	GCA_000188255	887324.3	649989900	887324	42.3

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Actinobacillus ureae strain Henrikson 3520/59 16S ribosomal RNA gene, partial sequence	M75075	1478	723
20218	Actinobacillus ureae ATCC 25976 strain CCUG 2139 16S ribosomal RNA gene, partial sequence	AY362900	1362	887324
20218	Actinobacillus ureae partial 16S rRNA gene, strain P161	AJ438663	435	723

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Actinobacillus ureae ATCC 25976
NCBI: GCA_000188255

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	98.00	no
125439	motility	BacteriaNetⓘ	no	52.80	no
125439	gram_stain	BacteriaNetⓘ	negative	97.00	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	95.60	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Actinobacillus ureae ATCC 25976 ATCC 25976
NCBI: GCA_000188255.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	99.00	no
125438	anaerobic	anaerobicⓘ	no	95.30	no
125438	aerobic	aerobicⓘ	no	77.88	no
125438	spore-forming	spore-formingⓘ	no	92.00	no
125438	thermophilic	thermophileⓘ	no	98.00	no
125438	flagellated	motile2+ⓘ	no	91.32	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Actinobacillus ureae: an unusual cause of tree-in-bud pattern in a case of pneumonia on lung computed tomographic scan-first clinical case report and review of the literature from India.	Dawar R, Nagarjuna D, Gupta R, Ghonge NP, Sardana R.	New Microbes New Infect	10.1016/j.nmni.2016.09.005	2016
Enzymology	Novel DNA Markers for Identification of Actinobacillus pleuropneumoniae.	Srijuntongsiri G, Mhoowai A, Samngamnim S, Assavacheep P, Bosse JT, Langford PR, Posayapisit N, Leartsakulpanich U, Songsungthong W.	Microbiol Spectr	10.1128/spectrum.01311-21	2022
	Nitrogen source influences the interactions of comammox bacteria with aerobic nitrifiers.	Vilardi KJ, Johnston J, Dai Z, Cotto I, Tuttle E, Patterson A, Stubbins A, Pieper KJ, Pinto AJ.	Microbiol Spectr	10.1128/spectrum.03181-23	2024
	Complete Genome Sequence of Nitrosomonas ureae Strain Nm10, an Oligotrophic Group 6a Nitrosomonad.	Kozlowski JA, Kits KD, Stein LY.	Genome Announc	10.1128/genomea.00094-16	2016
Transcriptome	A Transcriptome Map of Actinobacillus pleuropneumoniae at Single-Nucleotide Resolution Using Deep RNA-Seq.	Su Z, Zhu J, Xu Z, Xiao R, Zhou R, Li L, Chen H.	PLoS One	10.1371/journal.pone.0152363	2016
	Isolation, gene structure, and comparative analysis of the S-layer gene sslA of Sporosarcina ureae ATCC 13881.	Ryzhkov PM, Ostermann K, Rodel G.	Genetica	10.1007/s10709-006-9135-0	2007
	Targeting at the Nanoscale: A Novel S-Layer Fusion Protein Enabling Controlled Immobilization of Biotinylated Molecules.	Varga M.	Nanomaterials (Basel)	10.3390/nano6110199	2016
	Truncation Derivatives of the S-Layer Protein of Sporosarcina ureae ATCC 13881 (SslA): Towards Elucidation of the Protein Domain Responsible for Self-Assembly.	Varga M.	Molecules	10.3390/molecules21091117	2016
Phylogeny	Identification and classification of bacterial Type III toxin-antitoxin systems encoded in chromosomal and plasmid genomes.	Blower TR, Short FL, Rao F, Mizuguchi K, Pei XY, Fineran PC, Luisi BF, Salmond GP.	Nucleic Acids Res	10.1093/nar/gks231	2012
Enzymology	Rapid and accurate identification of human isolates of Pasteurella and related species by sequencing the sodA gene.	Gautier AL, Dubois D, Escande F, Avril JL, Trieu-Cuot P, Gaillot O.	J Clin Microbiol	10.1128/jcm.43.5.2307-2314.2005	2005
Metabolism	Production, secretion, and cell surface display of recombinant Sporosarcina ureae S-layer fusion proteins in Bacillus megaterium.	Knobloch D, Ostermann K, Rodel G.	Appl Environ Microbiol	10.1128/aem.06127-11	2012
Genetics	Comparative genomic analysis reveals distinct genotypic features of the emerging pathogen Haemophilus influenzae type f.	Su YC, Resman F, Horhold F, Riesbeck K.	BMC Genomics	10.1186/1471-2164-15-38	2014
Phylogeny	A Unique Capsule Locus in the Newly Designated Actinobacillus pleuropneumoniae Serovar 16 and Development of a Diagnostic PCR Assay.	Bosse JT, Li Y, Sarkozi R, Gottschalk M, Angen O, Nedbalcova K, Rycroft AN, Fodor L, Langford PR.	J Clin Microbiol	10.1128/jcm.02166-16	2017
Enzymology	Improved diagnostic PCR assay for Actinobacillus pleuropneumoniae based on the nucleotide sequence of an outer membrane lipoprotein.	Gram T, Ahrens P.	J Clin Microbiol	10.1128/jcm.36.2.443-448.1998	1998

References

#2224	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 5568
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#37608	; Curators of the CIP;
#44498	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 2139
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#68371	Automatically annotated from API 50CH acid .
#68382	Automatically annotated from API zym .
#123738	Collection of Institut Pasteur ; Curators of the CIP; CIP 62.8
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Actinobacillus ureae (DSM 5568, ATCC 25976, NCTC 10219)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Antibiotic susceptibility testing

Spore formation

Metabolite utilization

Antibiotic resistance

Metabolite production

Enzymes

API zym

API 50CHac

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000188255

16S sequences

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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