Name: Paenibacillus sputi DSM 22699
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 11650

Type strain:

Species: Paenibacillus sputi

Culture col. no.: DSM 22699, KCTC 13252, KIT 00200-70066-1, CIP 110216

Strain history: CIP <- 2010, KCTC

NCBI tax ID(s): 646095 (species)

SI-ID 371020

DSM 22699, KCTC 13252, CIP 110216

Section

Paenibacillus sputi DSM 22699 is a facultative anaerobe, spore-forming, mesophilic human pathogen that was isolated from sputum of a patient with pulmonary disease.

Gram-variable
motile
rod-shaped
spore-forming
facultative anaerobe
human pathogen
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Paenibacillaceae*
	Genus	*Paenibacillus*
	Species	**Paenibacillus sputi**
	Full Scientific Name (LPSN)	Paenibacillus sputi Kim et al. 2010

Information on morphological and physiological properties Morphology

[Ref.: #29620]	Gram stain	variable
[Ref.: #67771]	Gram stain	variable
[Ref.: #123282]	Gram stain	negative

[Ref.: #29620]	Cell length	02-03 µm

[Ref.: #29620]	Cell width	0.5-0.6 µm

[Ref.: #29620]	Cell shape	rod-shaped
[Ref.: #123282]	Cell shape	rod-shaped
[Ref.: #67771]	Cell shape	rod-shaped

[Ref.: #29620]	Motility	yes
[Ref.: #123282]	Motility	yes
[Ref.: #67771]	Motility	yes

[Ref.: #67771]	Flagellum arrangement	peritrichous

[Ref.: #29620]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #16470]

Culture medium

CASO AGAR (MERCK 105458) (DSMZ Medium 220)

[Ref.: #16470]

Culture medium growth

[Ref.: #16470]

Culture medium link

Medium recipe at MediaDive

[Ref.: #16470]

Culture medium composition

expand / minimize

[Ref.: #40867]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #40867]

Culture medium growth

[Ref.: #40867]

Culture medium composition

expand / minimize

[Ref.: #123282]

Culture medium

CIP Medium 72

[Ref.: #123282]

Culture medium growth

[Ref.: #123282]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #16470]	growth	30 °C
[Ref.: #29620]	growth	15-42 °C
[Ref.: #29620]	optimum	30-37 °C
[Ref.: #40867]	growth	30 °C
[Ref.: #67771]	growth	30-37 °C

[Ref.: #16470]	Temperature range	mesophilic
[Ref.: #29620]	Temperature range	mesophilic
[Ref.: #40867]	Temperature range	mesophilic
[Ref.: #67771]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #29620]			optimum	7
[Ref.: #29620]			growth	06-09

Information on physiology and metabolism Physiology and metabolism

[Ref.: #29620]	Oxygen tolerance	facultative anaerobe
[Ref.: #67771]	Oxygen tolerance	facultative anaerobe
[Ref.: #123282]	Oxygen tolerance	facultative anaerobe

[Ref.: #67771]	Type of spore	spore
[Ref.: #67771]	Ability of spore formation	yes
[Ref.: #29620]	Ability of spore formation	yes

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #29620]		NaCl		growth	0-3	%
[Ref.: #29620]		NaCl		optimum	0-3	%

[Ref.: #29620]	Observation	aggregates in chains
[Ref.: #67771]	Observation	quinones: MK-7

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #29620]	arabinose ChEBI	+	carbon source
[Ref.: #29620]	cellobiose ChEBI	+	carbon source
[Ref.: #68371]	cellobiose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fructose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-ribose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #29620]	esculin ChEBI	+	hydrolysis
[Ref.: #68371]	esculin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #29620]	galactose ChEBI	+	carbon source
[Ref.: #68371]	gluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #29620]	glucose ChEBI	+	carbon source
[Ref.: #29620]	glycogen ChEBI	+	carbon source
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #29620]	lactose ChEBI	+	carbon source
[Ref.: #68371]	lactose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #29620]	maltose ChEBI	+	carbon source
[Ref.: #68371]	maltose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	melezitose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melibiose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	N-acetylglucosamine ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #123282]	nitrate ChEBI	-	reduction
[Ref.: #123282]	nitrite ChEBI	-	reduction
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	raffinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #29620]	rhamnose ChEBI	+	carbon source
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #29620]	salicin ChEBI	+	carbon source
[Ref.: #68371]	salicin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	starch ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	sucrose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	trehalose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	turanose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #29620]	xylose ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #123282]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #29620]	catalase	+	1.11.1.6
[Ref.: #123282]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #29620]	cytochrome oxidase	+	1.9.3.1
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #123282]	oxidase	+
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #123282]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #123282]	11732	not determinedn.d.	+/-	-	+/-	+/-	-	+/-	-	-	+/-	+/-	+/-	-	-	-	+/-	-	+/-	-	-	+/-	+/-	-	+/-	+/-	+	+	+	+	+	+	-	-	-	-	-	+	+/-	-	+/-	-	-	-	-	+/-	-	-	-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #123282]	12443	-	-	+	+	-	+	-	-	-	-	+	+	-	+	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #16470]	Sample type/isolated from	sputum of a patient with pulmonary disease
[Ref.: #16470]	Geographic location (country and/or sea, region)	Seoul
[Ref.: #16470]	Country	Republic of Korea
[Ref.: #16470]	Country ISO 3 Code	KOR
[Ref.: #16470]	Continent	Asia

[Ref.: #67771]	Sample type/isolated from	From sputum of a patient with pulmonary disease
[Ref.: #67771]	Geographic location (country and/or sea, region)	Seoul
[Ref.: #67771]	Country	Republic of Korea
[Ref.: #67771]	Country ISO 3 Code	KOR
[Ref.: #67771]	Continent	Asia

[Ref.: #123282]	Sample type/isolated from	Human, Sputum
* marker position based on {}

Isolation sources categories

#Infection	#Inflammation	-
#Infection	#Patient	-
#Host Body-Site	#Oral cavity and airways	#Airways
#Host Body Product	#Fluids	#Sputum

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence FN394513 (>99% sequence identity) for Paenibacillus sputi subclade from Microbeatlas

Aquatic Samples	1
Soil Samples	9
Animal Samples	4
Plant Samples	2
Total Samples	16

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #16470]	Pathogenicity (human)	yes, in single cases
[Ref.: #16470]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #123282]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #16470]	Paenibacillus sputi partial 16S rRNA gene, type strain KIT 00200-70066-1T	FN394513	1457	ENA	646095 tax ID

[Ref.: #16470]	GC-content	48.1 mol%	high performance liquid chromatography (HPLC)
[Ref.: #67771]	GC-content	48.1 mol%

Availability in culture collections External links

[Ref.: #16470]

Culture collection no.

DSM 22699, KCTC 13252, KIT 00200-70066-1, CIP 110216

[Ref.: #80874]

SI-ID 371020

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Paenibacillus ripae sp. nov., isolated from bank side soil.	Sun Y, Guo Z, Zhao Q, Gao Q, Xie Q, Yang R, Liu R, Wu Z, Chen P, Li Y, Wang X, Li H	Int J Syst Evol Microbiol	10.1099/ijsem.0.000645	2015	*
Phylogeny	Paenibacillus sputi sp. nov., isolated from the sputum of a patient with pulmonary disease.	Kim KK, Lee KC, Yu H, Ryoo S, Park Y, Lee JS	Int J Syst Evol Microbiol	10.1099/ijs.0.017137-0	2009	*

References

#16470

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 22699

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#29620

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26010 (see below)

#40867 ; Curators of the CIP;
#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#68371

Automatically annotated from API 50CH acid .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#80874

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID371020.1 )

#123282 Collection of Institut Pasteur ; Curators of the CIP; CIP 110216

#26010

IJSEM 2371 2010 ( DOI 10.1099/ijs.0.017137-0 , PubMed 19946060 )

* These data were automatically processed and therefore are not curated

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