Name: Paenibacillus pueri b09i-3
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 11639

Type strain:

Species: Paenibacillus pueri

Strain Designation: b09i-3

Culture col. no.: DSM 22870, CECT 7360, KCTC 13223, CIP 110299

Strain history: CIP <- 2011, CECT <- 2008, K.S. Bae, KRIBB, Korea: strain b09i-3

NCBI tax ID(s): 512397 (species)

SI-ID 362825

CECT 7360, KCTC 13223, CIP 110299, DSM 22870

Section

Paenibacillus pueri b09i-3 is an anaerobe, spore-forming, mesophilic bacterium that was isolated from Pu’er tea .

Gram-positive
motile
rod-shaped
spore-forming
anaerobe
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Paenibacillaceae*
	Genus	*Paenibacillus*
	Species	**Paenibacillus pueri**
	Full Scientific Name (LPSN)	Paenibacillus pueri Kim et al. 2009

Information on morphological and physiological properties Morphology

[Ref.: #28946]	Gram stain	positive
[Ref.: #67771]	Gram stain	positive
[Ref.: #122042]	Gram stain	positive

[Ref.: #28946]	Cell length	3 µm

[Ref.: #28946]	Cell width	0.65 µm

[Ref.: #28946]	Cell shape	rod-shaped
[Ref.: #122042]	Cell shape	rod-shaped
[Ref.: #67771]	Cell shape	rod-shaped

[Ref.: #28946]	Motility	yes
[Ref.: #122042]	Motility	yes
[Ref.: #67771]	Motility	yes

[Ref.: #28946]

Pigment production

Information on culture and growth conditions Culture and growth conditions

[Ref.: #16592]

Culture medium

CASO AGAR (MERCK 105458) (DSMZ Medium 220)

[Ref.: #16592]

Culture medium growth

[Ref.: #16592]

Culture medium link

Medium recipe at MediaDive

[Ref.: #16592]

Culture medium composition

expand / minimize

[Ref.: #16592]

Culture medium

PLATE COUNT AGAR (DSMZ Medium 464)

[Ref.: #16592]

Culture medium growth

[Ref.: #16592]

Culture medium link

Medium recipe at MediaDive

[Ref.: #16592]

Culture medium composition

expand / minimize

[Ref.: #40503]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #40503]

Culture medium growth

[Ref.: #40503]

Culture medium composition

expand / minimize

[Ref.: #122042]

Culture medium

CIP Medium 72

[Ref.: #122042]

Culture medium growth

[Ref.: #122042]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #16592]	growth	30 °C
[Ref.: #28946]	growth	25-42 °C
[Ref.: #28946]	optimum	37 °C
[Ref.: #40503]	growth	30 °C
[Ref.: #67771]	growth	37 °C

[Ref.: #16592]	Temperature range	mesophilic
[Ref.: #28946]	Temperature range	mesophilic
[Ref.: #40503]	Temperature range	mesophilic
[Ref.: #67771]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #28946]			optimum	7.95
[Ref.: #28946]			growth	5.5-10.4

Information on physiology and metabolism Physiology and metabolism

[Ref.: #28946]	Oxygen tolerance	anaerobe
[Ref.: #67771]	Oxygen tolerance	aerobe
[Ref.: #122042]	Oxygen tolerance	obligate aerobe

[Ref.: #67771]	Type of spore	spore
[Ref.: #67771]	Ability of spore formation	yes
[Ref.: #28946]	Ability of spore formation	yes

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #28946]		NaCl		growth	0-6	%
[Ref.: #28946]		NaCl		optimum	3	%

[Ref.: #28946]	Observation	aggregates in clumps
[Ref.: #67771]	Observation	quinones: MK-7

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #28946]	5-dehydro-D-gluconate ChEBI	+	carbon source
[Ref.: #68371]	amygdalin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #28946]	arabinose ChEBI	+	carbon source
[Ref.: #68371]	arbutin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #28946]	cellobiose ChEBI	+	carbon source
[Ref.: #68371]	cellobiose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fructose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-galactose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-glucose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-ribose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-xylose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #28946]	esculin ChEBI	+	hydrolysis
[Ref.: #68371]	esculin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #28946]	fructose ChEBI	+	carbon source
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #28946]	galactose ChEBI	+	carbon source
[Ref.: #68371]	gentiobiose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #28946]	glucose ChEBI	+	carbon source
[Ref.: #28946]	glycerol ChEBI	+	carbon source
[Ref.: #68371]	glycerol ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycogen ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabinose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	lactose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #28946]	maltose ChEBI	+	carbon source
[Ref.: #68371]	maltose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #28946]	mannitol ChEBI	+	carbon source
[Ref.: #28946]	mannose ChEBI	+	carbon source
[Ref.: #68371]	melezitose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #28946]	melibiose ChEBI	+	carbon source
[Ref.: #68371]	melibiose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-glucopyranoside ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #28946]	myo-inositol ChEBI	+	carbon source
[Ref.: #68371]	N-acetylglucosamine ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #28946]	nitrate ChEBI	+	reduction
[Ref.: #122042]	nitrate ChEBI	-	reduction
[Ref.: #122042]	nitrite ChEBI	-	reduction
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #28946]	raffinose ChEBI	+	carbon source
[Ref.: #68371]	raffinose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #28946]	ribose ChEBI	+	carbon source
[Ref.: #28946]	salicin ChEBI	+	carbon source
[Ref.: #68371]	salicin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #28946]	sorbitol ChEBI	+	carbon source
[Ref.: #28946]	sucrose ChEBI	+	carbon source
[Ref.: #68371]	sucrose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #28946]	trehalose ChEBI	+	carbon source
[Ref.: #68371]	trehalose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	turanose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #28946]	tween 80 ChEBI	+	carbon source
[Ref.: #68371]	xylitol ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #28946]	xylose ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #28946]		indole ChEBI	yes
[Ref.: #122042]		indole ChEBI	no

	Physiological tests	Metabolite	Indole test
[Ref.: #28946]		indole ChEBI	+

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #28946]	alpha-galactosidase	+	3.2.1.22
[Ref.: #68382]	alpha-galactosidase	+	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #28946]	catalase	+	1.11.1.6
[Ref.: #122042]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #28946]	cytochrome oxidase	+	1.9.3.1
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #28946]	gelatinase	+
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #122042]	oxidase	+
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #122042]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #122042]	11743	not determinedn.d.	+	-	+/-	+	+	+	-	-	+	+	+	+	+/-	-	+	-	+/-	+	+	+	+	-	+	-	+	+	+	+	+	+	+	+	-	+	+	+/-	-	+	+	+	-	-	-	+/-	-	-	+/-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #122042]	12501	-	+	+	-	-	+	-	-	-	-	-	+	+	+	-	+	+	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #16592]	Sample type/isolated from	Pu’er tea (tea plant Camellia sinensis)
[Ref.: #16592]	Host species	Camellia sinensis
[Ref.: #16592]	Country	China
[Ref.: #16592]	Country ISO 3 Code	CHN
[Ref.: #16592]	Continent	Asia

[Ref.: #67771]	Sample type/isolated from	From Pu'er tea, tea plant `Camellia sinensis`
[Ref.: #67771]	Country	China
[Ref.: #67771]	Country ISO 3 Code	CHN
[Ref.: #67771]	Continent	Asia

[Ref.: #122042]	Sample type/isolated from	Plant, Tea plant, Camellia sinensis
[Ref.: #122042]	Country	China
[Ref.: #122042]	Country ISO 3 Code	CHN
[Ref.: #122042]	Continent	Asia
[Ref.: #122042]	Isolation date	2005
* marker position based on {}

Isolation sources categories

#Engineered	#Food production	#Beverage
#Host	#Plants	#Shrub (Scrub)

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #16592]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #122042]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #16592]	Paenibacillus pueri strain b09i-3 16S ribosomal RNA gene, partial sequence	EU391156	1456	ENA	512397 tax ID

[Ref.: #16592]

GC-content

56.6 mol%

Availability in culture collections External links

[Ref.: #16592]

Culture collection no.

DSM 22870, CECT 7360, KCTC 13223, CIP 110299

[Ref.: #80863]

SI-ID 362825

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Paenibacillus pueri sp. nov., isolated from Pu'er tea.	Kim BC, Jeong WJ, Kim DY, Oh HW, Kim H, Park DS, Park HM, Bae KS	Int J Syst Evol Microbiol	10.1099/ijs.0.002352-0	2009	*

References

#16592

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 22870

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#28946

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #25385 (see below)

#40503 ; Curators of the CIP;
#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#68371

Automatically annotated from API 50CH acid .

#68382

Automatically annotated from API zym .

#80863

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID362825.1 )

#122042 Collection of Institut Pasteur ; Curators of the CIP; CIP 110299

#25385

IJSEM 1002 2009 ( DOI 10.1099/ijs.0.002352-0 , PubMed 19406782 )

* These data were automatically processed and therefore are not curated

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