Name: Paenibacillus sabinae T27
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 11620

Type strain:

Species: Paenibacillus sabinae

Strain Designation: T27

Culture col. no.: DSM 17841, CCBAU 10202, CIP 109632, KCTC 13697

Strain history: CIP <- 2007, DSMZ <- Y. Ma, Beijing Univ., Beijing, China: strain T27

NCBI tax ID(s): 365617 (species)

SI-ID 297290

DSM 17841, CIP 109632

Section

Paenibacillus sabinae T27 is an anaerobe, spore-forming, mesophilic bacterium that was isolated from plant rhizosphere .

Gram-positive
motile
rod-shaped
spore-forming
anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Paenibacillaceae*
	Genus	*Paenibacillus*
	Species	**Paenibacillus sabinae**
	Full Scientific Name (LPSN)	Paenibacillus sabinae Ma et al. 2007

Information on morphological and physiological properties Morphology

[Ref.: #31848]	Gram stain	positive
[Ref.: #122326]	Gram stain	positive

[Ref.: #31848]	Cell length	2.95 µm

[Ref.: #31848]	Cell width	0.75 µm

[Ref.: #31848]	Cell shape	rod-shaped
[Ref.: #122326]	Cell shape	rod-shaped

[Ref.: #31848]	Motility	yes
[Ref.: #122326]	Motility	yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #7136]

Culture medium

CASO AGAR (MERCK 105458) (DSMZ Medium 220)

[Ref.: #7136]

Culture medium growth

[Ref.: #7136]

Culture medium link

Medium recipe at MediaDive

[Ref.: #7136]

Culture medium composition

expand / minimize

[Ref.: #37796]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #37796]

Culture medium growth

[Ref.: #37796]

Culture medium composition

expand / minimize

[Ref.: #122326]

Culture medium

CIP Medium 72

[Ref.: #122326]

Culture medium growth

[Ref.: #122326]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #7136]	growth	30 °C
[Ref.: #31848]	growth	04-37 °C
[Ref.: #31848]	optimum	30 °C
[Ref.: #37796]	growth	30 °C

[Ref.: #7136]	Temperature range	mesophilic
[Ref.: #31848]	Temperature range	mesophilic
[Ref.: #37796]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #31848]			optimum	7.2
[Ref.: #31848]			growth	4.2-10

Information on physiology and metabolism Physiology and metabolism

[Ref.: #31848]	Oxygen tolerance	anaerobe
[Ref.: #122326]	Oxygen tolerance	facultative anaerobe

[Ref.: #31848]

Ability of spore formation

yes

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #31848]		NaCl		growth	0-3	%
[Ref.: #31848]		NaCl		optimum	1.5	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68371]	cellobiose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fructose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-galactose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-glucose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-ribose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	esculin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gentiobiose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	gluconate ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycerol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycogen ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	lactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	maltose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	melezitose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	melibiose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	N-acetylglucosamine ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31848]	nitrate ChEBI	+	reduction
[Ref.: #122326]	nitrate ChEBI	-	reduction
[Ref.: #122326]	nitrite ChEBI	-	reduction
[Ref.: #68371]	raffinose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	salicin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	starch ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	sucrose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	trehalose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	turanose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #122326]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	+	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #31848]	catalase	+	1.11.1.6
[Ref.: #122326]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #68382]	leucine arylamidase	-	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #122326]	oxidase	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #122326]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #122326]	11620	not determinedn.d.	-	-	-	-	-	-	-	-	-	+	+	+	+	-	-	-	-	+	+/-	-	+/-	-	+/-	+/-	+	+	+	+	-	+	+	+	+/-	+	+	+	+	-	+	+	-	-	-	-	-	-	+	+/-	+/-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #122326]	12015	-	-	+	-	-	-	-	-	-	-	-	-	+	-	-	+	+	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #7136]	Sample type/isolated from	plant rhizosphere (Sabina squamata)
[Ref.: #7136]	Host species	Sabina squamata
[Ref.: #7136]	Geographic location (country and/or sea, region)	Beijing
[Ref.: #7136]	Country	China
[Ref.: #7136]	Country ISO 3 Code	CHN
[Ref.: #7136]	Continent	Asia

[Ref.: #122326]	Sample type/isolated from	Plant, Rhizosphere
[Ref.: #122326]	Country	China
[Ref.: #122326]	Country ISO 3 Code	CHN
[Ref.: #122326]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Host Body-Site	#Plant	#Rhizosphere

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence DQ338444 (>99% sequence identity) for Paenibacillus sabinae from Microbeatlas

Aquatic Samples	6
Soil Samples	25
Animal Samples	8
Plant Samples	25
Total Samples	64

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #7136]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #122326]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #31848]	Paenibacillus sabinae strain T27 16S ribosomal RNA gene, partial sequence	DQ338444	1519	GenBank	365617 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Paenibacillus sabinae T27	GCA_000612505	complete	GenBank	1268072 tax ID	*
[Ref.: #66792]	Paenibacillus sabinae T27	1268072.3	complete	PATRIC	1268072 tax ID	*
[Ref.: #66792]	Paenibacillus sabinae T27	2576861424	complete	JGI IMG/M	1268072 tax ID	*

[Ref.: #7136]

GC-content

51.9 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Paenibacillus sabinae T27 T27 NCBI: GCA_000612505.1
[Ref.: #69480]	motile	yes	89.212	no
[Ref.: #69480]	flagellated	yes	88.001	no
[Ref.: #69480]	gram-positive	yes	89.044	yes
[Ref.: #69480]	anaerobic	no	79.059	no
[Ref.: #69480]	halophile	no	95.982	no
[Ref.: #69480]	spore-forming	yes	95.596	yes
[Ref.: #69480]	thermophile	no	99.185	yes
[Ref.: #69480]	glucose-util	yes	87.764	no
[Ref.: #69480]	aerobic	no	52.195	yes
[Ref.: #69480]	glucose-ferment	no	80.762	no

Availability in culture collections External links

[Ref.: #7136]

Culture collection no.

DSM 17841, CCBAU 10202, CIP 109632, KCTC 13697

[Ref.: #80846]

SI-ID 297290

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Paenibacillus brassicae sp. nov., isolated from cabbage rhizosphere in Beijing, China.	Gao M, Yang H, Zhao J, Liu J, Sun YH, Wang YJ, Sun JG	Antonie Van Leeuwenhoek	10.1007/s10482-012-9849-1	2012	*
Enzymology	Characterization and analysis of nifH genes from Paenibacillus sabinae T27.	Hong Y, Ma Y, Wu L, Maki M, Qin W, Chen S	Microbiol Res	10.1016/j.micres.2012.05.003	2012	*
Phylogeny	Paenibacillus sophorae sp. nov., a nitrogen-fixing species isolated from the rhizosphere of Sophora japonica.	Jin HJ, Lv J, Chen SF	Int J Syst Evol Microbiol	10.1099/ijs.0.021709-0	2010	*
Phylogeny	Paenibacillus forsythiae sp. nov., a nitrogen-fixing species isolated from rhizosphere soil of Forsythia mira.	Ma YC, Chen SF	Int J Syst Evol Microbiol	10.1099/ijs.0.65238-0	2008	*
Phylogeny	Paenibacillus sabinae sp. nov., a nitrogen-fixing species isolated from the rhizosphere soils of shrubs.	Ma Y, Xia Z, Liu X, Chen S	Int J Syst Evol Microbiol	10.1099/ijs.0.64519-0	2007	*

References

#7136

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 17841

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#31848

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #28112 (see below)

#37796 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68371

Automatically annotated from API 50CH acid .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#80846

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID297290.1 )

#122326 Collection of Institut Pasteur ; Curators of the CIP; CIP 109632

#28112

IJSEM 6 2007 ( DOI 10.1099/ijs.0.64519-0 , PubMed 17220432 )

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Paenibacillus sabinae in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF

Paenibacillus sabinae T27 T27

Change proposal

Change proposal