Paenibacillus barengoltzii (DSM 22255, ATCC BAA-1209, CIP 109354)

Name: Paenibacillus barengoltzii (DSM 22255, ATCC BAA-1209, CIP 109354)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 11612

Type strain

Strain Designation SAFN-016

Culture col. no. DSM 22255 ATCC BAA-1209 CIP 109354 NBRC 101215 SAFN-016

NCBI tax ID(s) 1220556 343517

Special Collections DSMZ CIP Literature strains

History DSMZ <- M. Miyashita, Natl. Inst. of Technol. and Evaluation (NITE), Japan <- K. Venkateswaran; SAFN-016 CIP <- 2006, NBRC <- M. Satomi, Nat. Res. Inst. Fish. Sci., Yokohama, Japan: strain SAFN-016 <- K. Venkateswaran

Links

Paenibacillus barengoltzii SAFN-016 is an aerobe, spore-forming, Gram-positive bacterium that was isolated from clean room floors of the Jet Propulsion Laboratory Spacecraft Assembly Facility .

spore-forming Gram-positive motile rod-shaped aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 297279

ATCC BAA-1209,NBRC 101215,CIP 109354,DSM 22255

@ref 20215

Last LPSN update 2026-05-29 08:27:36

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Caryophanales

Family Paenibacillaceae

Genus Paenibacillus

Species Paenibacillus barengoltzii

Full scientific name Paenibacillus barengoltzii Osman et al. 2006

BacDive ID	Other strains from **Paenibacillus barengoltzii** (6)	Type strain
11613	P. barengoltzii ES-MS41, DSM 30773
153029	P. barengoltzii CCUG 48350
153090	P. barengoltzii CCUG 48450
154574	P. barengoltzii CCUG 54451
156702	P. barengoltzii CCUG 64230
156977	P. barengoltzii CCUG 67940 Q

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Motility
31703	positive	03-05 µm	0.5-0.8 µm	rod-shaped
116734	positive			rod-shaped

Pigmentation

31703

Productionyes

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
16237	CASO AGAR (MERCK 105458) (DSMZ Medium 220)	Medium recipe at MediaDive	Name: CASO AGAR (Merck 105458) (DSMZ Medium 220; with strain-specific modifications) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l MnSO4 0.01 g/l Distilled water
37525	MEDIUM 72- for trypto casein soja agar		Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
116734	CIP Medium 72	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
16237	positive	growth	30
31703	positive	growth	10-50
31703	positive	optimum	37
37525	positive	growth	30

Culture pH

@ref	Ability	Type	PH	PH range
31703	positive	optimum	7
31703	positive	growth	4.5-9	alkaliphile

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
31703	aerobe
116734	facultative anaerobe
125439	facultative anaerobe	92.682

Spore formation

@ref	Spore formation	Confidence
31703
125439		96.702
125438		94.526
116734

Halophily

@ref	Salt	Growth	Tested relation	Concentration
31703	NaCl	positive	growth	2 %
31703	NaCl	positive	optimum	2 %

Observation

31703

Observationaggregates in chains

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68371	27613 ChEBI	amygdalin	+	builds acid from	from API 50CH acid
68371	18305 ChEBI	arbutin	+	builds acid from	from API 50CH acid
68371	17057 ChEBI	cellobiose	+	builds acid from	from API 50CH acid
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
68371	15824 ChEBI	D-fructose	+	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	12936 ChEBI	D-galactose	+	builds acid from	from API 50CH acid
68371	17634 ChEBI	D-glucose	+	builds acid from	from API 50CH acid
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68371	16899 ChEBI	D-mannitol	+	builds acid from	from API 50CH acid
68371	16024 ChEBI	D-mannose	+	builds acid from	from API 50CH acid
68371	16988 ChEBI	D-ribose	-	builds acid from	from API 50CH acid
68371	17924 ChEBI	D-sorbitol	-	builds acid from	from API 50CH acid
68371	16443 ChEBI	D-tagatose	-	builds acid from	from API 50CH acid
68371	65327 ChEBI	D-xylose	+	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
31703	4853 ChEBI	esculin	+	hydrolysis
68371	4853 ChEBI	esculin	+	builds acid from	from API 50CH acid
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
68371	28066 ChEBI	gentiobiose	+	builds acid from	from API 50CH acid
31703	24265 ChEBI	gluconate	+	carbon source
68371	24265 ChEBI	gluconate	+	builds acid from	from API 50CH acid
68371	17754 ChEBI	glycerol	-	builds acid from	from API 50CH acid
68371	28087 ChEBI	glycogen	+	builds acid from	from API 50CH acid
68371	15443 ChEBI	inulin	+	builds acid from	from API 50CH acid
68371	30849 ChEBI	L-arabinose	+	builds acid from	from API 50CH acid
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	+	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	+	builds acid from	from API 50CH acid
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
68371	17716 ChEBI	lactose	+	builds acid from	from API 50CH acid
68371	17306 ChEBI	maltose	+	builds acid from	from API 50CH acid
68371	6731 ChEBI	melezitose	-	builds acid from	from API 50CH acid
68371	28053 ChEBI	melibiose	+	builds acid from	from API 50CH acid
68371	320061 ChEBI	methyl alpha-D-glucopyranoside	+	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	+	builds acid from	from API 50CH acid
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
68371	59640 ChEBI	N-acetylglucosamine	+	builds acid from	from API 50CH acid
31703	17632 ChEBI	nitrate	+	reduction
116734	17632 ChEBI	nitrate	+	reduction
116734	16301 ChEBI	nitrite	-	reduction
68371	0 ChEBI	Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371	0 ChEBI	Potassium 5-ketogluconate	-	builds acid from	from API 50CH acid
68371	16634 ChEBI	raffinose	+	builds acid from	from API 50CH acid
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
68371	17814 ChEBI	salicin	+	builds acid from	from API 50CH acid
68371	28017 ChEBI	starch	+	builds acid from	from API 50CH acid
68371	17992 ChEBI	sucrose	+	builds acid from	from API 50CH acid
68371	27082 ChEBI	trehalose	+	builds acid from	from API 50CH acid
68371	32528 ChEBI	turanose	+	builds acid from	from API 50CH acid
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

Metabolite production

@ref	Chebi-ID	Metabolite	Production
116734	35581 ChEBI	indole

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	+	3.2.1.51	from API zym
68382	alpha-galactosidase	+	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	+	3.2.1.24	from API zym
116734	amylase	+
68382	beta-galactosidase	+	3.2.1.23	from API zym
68382	beta-glucosidase	+	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
31703	catalase	+	1.11.1.6
116734	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
31703	cytochrome oxidase	+	1.9.3.1
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
68382	leucine arylamidase	-	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
116734	oxidase	+
68382	trypsin	-	3.4.21.4	from API zym
116734	tween esterase	-
116734	urease	-	3.5.1.5
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
116734	-	-	+	+	-	-	-	-	-	-	+	+	+	+	-	+	+	+	+	+

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
116734	not determinedn.d.	-	-	-	+	-	+	-	-	+	+	+	+	+	-	+	-	-	+	-	+/-	+	+	+	+	+	+	+	+	+	+	+	+	+	-	+	+	+	-	+	+	-	-	-	+	-	-	+	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Built environment	#Clean room

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
16237	clean room floors of the Jet Propulsion Laboratory Spacecraft Assembly Facility (JPL-SAF)	California, Pasadena	USA	USA	North America
116734	Clean room floor, spacecraft assembly facility	Pasadena, California	United States of America	USA	North America

Taxonmaps

69479

Global distribution of 16S sequence AB681405 (>99% sequence identity) for Paenibacillus barengoltzii subclade from Microbeatlas

Aquatic Samples	39
Soil Samples	164
Animal Samples	185
Plant Samples	110
Total Samples	498

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
16237	1	Risk group (German classification) According to TRBA 466
116734	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	NCBI tax ID	Score
66792	ASM400074v1 assembly for Paenibacillus barengoltzii NBRC 101215	contig	GCA_004000745	1220556.3	1220556	46.02

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Paenibacillus barengoltzii gene for 16S rRNA, partial sequence, strain: NBRC 101215	AB681405	1478	343517
16237	Paenibacillus barengoltzii strain SAFN-016 16S ribosomal RNA gene, partial sequence	AY167814	1339	343517
124043	Paenibacillus barengoltzii strain NBRC101215 16S ribosomal RNA gene, partial sequence.	KP704353	1416	343517

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM400074v1 assembly for Paenibacillus barengoltzii NBRC 101215
NCBI: GCA_004000745

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	facultative anaerobe	92.68	no
125439	motility	BacteriaNetⓘ	yes	87.05	no
125439	gram_stain	BacteriaNetⓘ	variable	74.55	no
125439	spore_formation	BacteriaNetⓘ	yes	96.70	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Paenibacillus barengoltzii NBRC 101215
PATRIC: 1220556.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	68.58	yes
125438	anaerobic	anaerobicⓘ	no	91.60	no
125438	spore-forming	spore-formingⓘ	yes	94.53	yes
125438	aerobic	aerobicⓘ	no	57.40	no
125438	thermophilic	thermophileⓘ	no	91.87	no
125438	flagellated	motile2+ⓘ	yes	83.97	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Visualizing and Isolating Iron-Reducing Microorganisms at the Single-Cell Level.	Gan C, Wu R, Luo Y, Song J, Luo D, Li B, Yang Y, Xu M.	Appl Environ Microbiol	10.1128/aem.02192-20	2021
	Characterization of Tomato Seed Endophytic Bacteria as Growth Promoters and Potential Biocontrol Agents.	Roy M, Kang B, Yang S, Choi H, Choi K.	Plant Pathol J	10.5423/ppj.oa.09.2024.0142	2024
Phylogeny	Characterization of a microbial consortium capable of degrading lignocellulose.	Wang W, Yan L, Cui Z, Gao Y, Wang Y, Jing R	Bioresour Technol	10.1016/j.biortech.2011.07.065	2011
	Paenibacillus rhizolycopersici sp. nov., an oligotrophic bacterium isolated from a tomato plant in China.	Thin KK, He SW, Ma R, Wang X, Han JG, Zhang XX.	Int J Syst Evol Microbiol	10.1099/ijsem.0.005698	2023
Genetics	Paenibacillus phocaensis sp. nov., isolated from the gut microbiota of a healthy infant.	Tidjani Alou M, Rathored J, Nguyen TT, Andrieu C, Couderc C, Brah S, Diallo BA, Fournier PE, Raoult D, Dubourg G.	New Microbes New Infect	10.1016/j.nmni.2016.12.001	2017
Phylogeny	Paenibacillus phoenicis sp. nov., isolated from the Phoenix Lander assembly facility and a subsurface molybdenum mine.	Benardini JN, Vaishampayan PA, Schwendner P, Swanner E, Fukui Y, Osman S, Satomi M, Venkateswaran K.	Int J Syst Evol Microbiol	10.1099/ijs.0.021428-0	2011
Phylogeny	Biological Synthesis of Ginsenoside Rd Using Paenibacillus horti sp. nov. Isolated from Vegetable Garden.	Akter S, Huq MA	Curr Microbiol	10.1007/s00284-018-1561-6	2018
Phylogeny	Paenibacillus cisolokensis sp. nov., isolated from litter of a geyser.	Yokota A, Ningsih F, Nurlaili DG, Sakai Y, Yabe S, Oetari A, Santoso I, Sjamsuridzal W	Int J Syst Evol Microbiol	10.1099/ijsem.0.001151	2016
Phylogeny	Paenibacillus cathormii sp. nov., isolated from tree bark.	Sitdhipol J, Paek J, Sin Y, Park IS, Thamacharoensuk T, Wannissorn B, Tanasupawat S, Chang YH	Int J Syst Evol Microbiol	10.1099/ijsem.0.000854	2015
Phylogeny	Paenibacillus faecis sp. nov., isolated from human faeces.	Clermont D, Gomard M, Hamon S, Bonne I, Fernandez JC, Wheeler R, Malosse C, Chamot-Rooke J, Gribaldo S, Boneca IG, Bizet C	Int J Syst Evol Microbiol	10.1099/ijsem.0.000622	2015
Phylogeny	Paenibacillus fonticola sp. nov., isolated from a warm spring.	Chou JH, Chou YJ, Lin KY, Sheu SY, Sheu DS, Arun AB, Young CC, Chen WM	Int J Syst Evol Microbiol	10.1099/ijs.0.64872-0	2007
Phylogeny	Paenibacillus pasadenensis sp. nov. and Paenibacillus barengoltzii sp. nov., isolated from a spacecraft assembly facility.	Osman S, Satomi M, Venkateswaran K	Int J Syst Evol Microbiol	10.1099/ijs.0.64085-0	2006
Phylogeny	Paenibacillus mellifer sp. nov., isolated from gut of the honey bee Apis mellifera.	Cho ES, Hwang CY, Kwon HW, Seo MJ	Arch Microbiol	10.1007/s00203-022-03178-0	2022

References

#16237	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 22255
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#27980	IJSEM 1509 2006 ( DOI 10.1099/ijs.0.64085-0 , PubMed 16825621 )
#31703	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27980
#37525	; Curators of the CIP;
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#68371	Automatically annotated from API 50CH acid .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#116734	Collection of Institut Pasteur ; Curators of the CIP; CIP 109354
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Content

About us

Paenibacillus barengoltzii (DSM 22255, ATCC BAA-1209, CIP 109354)

Name and taxonomic classification

Morphology

Cell morphology

Pigmentation

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Observation

Metabolite utilization

Metabolite production

Enzymes

API zym

API 50CHac

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_004000745

16S sequences

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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