Name: Paenibacillus larvae Med-540
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 11592

Type strain:

Species: Paenibacillus larvae

Strain Designation: Med-540, 846

Culture col. no.: CCUG 28515, CIP 104618, DSM 7030, ATCC 9545, LMG 9820, NRRL B-2605

Strain history: CIP <- 1995, ATCC <- E.C. Holst: strain 846

NCBI tax ID(s): 1464 (species)

SI-ID 1126

LMG 9820, ATCC 9545, CCRC 14187, DSM 7030, LMG 15969, NRRL B-2605, CCUG 28515, CIP 104618, KCTC 3744, BCRC 14187

Other strains from Paenibacillus larvae

Section

Paenibacillus larvae Med-540 is a microaerophile, spore-forming, mesophilic animal pathogen that was isolated from foul brood of honeybees.

Gram-positive
spore-forming
animal pathogen
mesophilic
microaerophile
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Paenibacillaceae*
	Genus	*Paenibacillus*
	Species	**Paenibacillus larvae**
	Full Scientific Name (LPSN)	Paenibacillus larvae (White 1906) Ash et al. 1994

	Synonym	Bacillus larvae
	Synonym	Paenibacillus pulvifaciens
	Synonym	Bacillus pulvifaciens
	Synonym	Paenibacillus larvae subsp. pulvifaciens

Information on morphological and physiological properties Morphology

[Ref.: #120564]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #120564]	Cell shape	rod-shaped

[Ref.: #120564]	Motility	yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #2955]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #2955]

Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

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Culture medium

J - AGAR (DSMZ Medium 262)

[Ref.: #2955]

Culture medium growth

[Ref.: #2955]

Culture medium link

Medium recipe at MediaDive

[Ref.: #2955]

Culture medium composition

expand / minimize

[Ref.: #38668]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #38668]

Culture medium growth

[Ref.: #38668]

Culture medium composition

expand / minimize

[Ref.: #120564]

Culture medium

CIP Medium 72

[Ref.: #120564]

Culture medium growth

[Ref.: #120564]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #120564]

Culture medium

CIP Medium 29

[Ref.: #120564]

Culture medium growth

[Ref.: #120564]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #2955]		growth	28 °C
[Ref.: #38668]		growth	30 °C
[Ref.: #49263]		growth	37 °C
[Ref.: #120564]		growth	30 °C
[Ref.: #120564]	no	growth	10 °C
[Ref.: #120564]	no	growth	45 °C
[Ref.: #120564]	no	growth	55 °C

[Ref.: #2955]	Temperature range	mesophilic
[Ref.: #38668]	Temperature range	mesophilic
[Ref.: #49263]	Temperature range	mesophilic
[Ref.: #120564]	Temperature range	mesophilic
[Ref.: #120564]	Temperature range	psychrophilic
[Ref.: #120564]	Temperature range	thermophilic

	pH	Kind of pH		pH
[Ref.: #120564]			growth	6

Information on physiology and metabolism Physiology and metabolism

[Ref.: #2955]	Oxygen tolerance	microaerophile
[Ref.: #49263]	Oxygen tolerance	aerobe
[Ref.: #120564]	Oxygen tolerance	facultative anaerobe

[Ref.: #120564]	Ability of spore formation	yes
[Ref.: #69481]	Ability of spore formation	yes Confidence in %100
[Ref.: #69480]	Ability of spore formation	yes Confidence in %100

	Salt	Tested relation		Salt conc.
[Ref.: #120564]	NaCl		growth	0-4	%
[Ref.: #120564]	NaCl	no	growth	6	%
[Ref.: #120564]	NaCl	no	growth	8	%
[Ref.: #120564]	NaCl	no	growth	10	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #120564]	citrate ChEBI	-	carbon source
[Ref.: #120564]	nitrate ChEBI	+	reduction
[Ref.: #120564]	nitrate ChEBI	+	respiration
[Ref.: #120564]	nitrite ChEBI	-	reduction

	Metabolite production	Metabolite	Production
[Ref.: #120564]		indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #120564]		acetoin ChEBI	-

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #120564]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #120564]	caseinase	+	3.4.21.50
[Ref.: #120564]	catalase	-	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #120564]	DNase	+
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #120564]	gelatinase	+/-
[Ref.: #120564]	lecithinase	-
[Ref.: #68382]	leucine arylamidase	-	3.4.11.1	* from API zym
[Ref.: #120564]	lipase	+
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #120564]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #120564]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #120564]	oxidase	+
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #120564]	tween esterase	-
[Ref.: #120564]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #120564]	8080	-	+	+	+	-	-	-	-	-	-	+	+	-	-	-	-	-	+	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #2955]	Sample type/isolated from	foul brood of honeybees

[Ref.: #49263]	Sample type/isolated from	Foul brood of honeybees

[Ref.: #120564]	Sample type/isolated from	Honeybees, foul brood
* marker position based on {}

Isolation sources categories

#Infection	#Disease	-
#Host	#Arthropoda	#Insecta

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AB073205 (>99% sequence identity) for Paenibacillus larvae subclade from Microbeatlas

Aquatic Samples	749
Soil Samples	4373
Animal Samples	3706
Plant Samples	544
Total Samples	9372

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #2955]	Pathogenicity (animal)	yes
[Ref.: #2955]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #120564]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Paenibacillus larvae clone BLARV-1F 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence	AF478106	474	ENA	1464 tax ID	*
[Ref.: #20218]	Paenibacillus larvae 16s ribosomal RNA, partial sequence	U85263	797	ENA	1464 tax ID	*
[Ref.: #20218]	B.larvae 16S ribosomal RNA	X60619	1437	ENA	1464 tax ID	*
[Ref.: #20218]	Paenibacillus larvae gene for 16S rRNA, partial sequence	AB073205	1506	ENA	1464 tax ID	*
[Ref.: #20218]	Paenibacillus larvae subsp. larvae strain DSM 7030 16S ribosomal RNA gene, complete sequence	AY530294	1515	ENA	147375 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Paenibacillus larvae subsp. larvae ATCC-9545	GCA_002003265	complete	GenBank	147375 tax ID	*
[Ref.: #66792]	Paenibacillus larvae subsp. larvae strain ATCC 9545	147375.4	complete	PATRIC	147375 tax ID	*
[Ref.: #66792]	Paenibacillus larvae subsp. larvae strain ERIC_I	147375.5	complete	PATRIC	147375 tax ID	*
[Ref.: #66792]	Paenibacillus larvae larvae ATCC 9545	2814123210	complete	JGI IMG/M	147375 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Paenibacillus larvae subsp. larvae strain ERIC_I PATRIC: 147375.5
[Ref.: #69481]	spore-forming	yes	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Paenibacillus larvae subsp. larvae ATCC 9545 NCBI: GCA_002003265.1
[Ref.: #69480]	flagellated	yes	63.632	no
[Ref.: #69480]	gram-positive	yes	81.43	no
[Ref.: #69480]	anaerobic	no	97.933	yes
[Ref.: #69480]	aerobic	yes	89.099	no
[Ref.: #69480]	halophile	no	92.719	no
[Ref.: #69480]	spore-forming	yes	97.196	no
[Ref.: #69480]	glucose-util	yes	89.073	no
[Ref.: #69480]	thermophile	no	85.575	yes
[Ref.: #69480]	motile	yes	82.993	no
[Ref.: #69480]	glucose-ferment	no	87.27	no

Availability in culture collections External links

[Ref.: #2955]

Culture collection no.

CCUG 28515, CIP 104618, DSM 7030, ATCC 9545, LMG 9820, NRRL B-2605

[Ref.: #80820]

SI-ID 1126

Literature:

Only first 10 entries are displayed. Click here to see all.

	Title	Authors	Journal	DOI	Year
Cultivation	Recording the Presence of Peanibacillus larvae larvae Colonies on MYPGP Substrates Using a Multi-Sensor Array Based on Solid-State Gas Sensors.	Bak B, Wilk J, Artiemjew P, Wilde J	Sensors (Basel)	10.3390/s21144917	2021	*
Phylogeny	Isolation of Paenibacillus tumbae sp. nov., from the tomb of the emperor Yang of the Sui dynasty, and emended description of the genus Paenibacillus.	Huang Z, Zhao F, Li YH	Antonie Van Leeuwenhoek	10.1007/s10482-016-0807-1	2016	*
Pathogenicity	Antimicrobial activity of Amazonian oils against Paenibacillus species.	Santos RC, dos Santos Alves CF, Schneider T, Lopes LQ, Aurich C, Giongo JL, Brandelli A, de Almeida Vaucher R	J Invertebr Pathol	10.1016/j.jip.2011.12.002	2011	*
Phylogeny	Isolation and characterization of a protective bacterial culture isolated from honey active against American Foulbrood disease.	Lee H, Churey JJ, Worobo RW	FEMS Microbiol Lett	10.1111/j.1574-6968.2009.01615.x	2009	*
Phylogeny	Reclassification of Paenibacillus larvae subsp. pulvifaciens and Paenibacillus larvae subsp. larvae as Paenibacillus larvae without subspecies differentiation.	Genersch E, Forsgren E, Pentikainen J, Ashiralieva A, Rauch S, Kilwinski J, Fries I	Int J Syst Evol Microbiol	10.1099/ijs.0.63928-0	2006	*
Phylogeny	Inhibition of the growth of Paenibacillus larvae, the causal agent of American foulbrood of honeybees, by selected strains of aerobic spore-forming bacteria isolated from apiarian sources.	Alippi AM, Reynaldi FJ	J Invertebr Pathol	10.1016/j.jip.2005.12.002	2006	*
Genetics	Strain- and genotype-specific differences in virulence of Paenibacillus larvae subsp. larvae, a bacterial pathogen causing American foulbrood disease in honeybees.	Genersch E, Ashiralieva A, Fries I	Appl Environ Microbiol	10.1128/AEM.71.11.7551-7555.2005	2005	*
Genetics	Biochemical characterization of different genotypes of Paenibacillus larvae subsp. larvae, a honey bee bacterial pathogen.	Neuendorf S, Hedtke K, Tangen G, Genersch E	Microbiology (Reading)	10.1099/mic.0.27125-0	2004	*
Enzymology	Biological properties and antibiotic susceptibility of Bacillus larvae originated from American foulbrood of honeybee in Japan.	Okayama A, Sakogawa T, Nakajima C, Hayama T	J Vet Med Sci	10.1292/jvms.58.439	1996	*
Phylogeny	Proposal to reclassify Paenibacillus larvae subsp. pulvifaciens DSM 3615 (ATCC 49843) as Paenibacillus larvae subsp. larvae. Results of a comparative biochemical and genetic study.	Kilwinski J, Peters M, Ashiralieva A, Genersch E	Vet Microbiol	10.1016/j.vetmic.2004.08.001	2004	*
Phylogeny	Reclassification of Paenibacillus (formerly Bacillus) pulvifaciens (Nakamura 1984) Ash et al. 1994, a later subjective synonym of Paenibacillus (formerly Bacillus) larvae (White 1906) Ash et al. 1994, as a subspecies of P. larvae, with emended descriptions of P. larvae as P. larvae subsp. larvae and P. larvae subsp. pulvifaciens.	Heyndrickx M, Vandemeulebroecke K, Hoste B, Janssen P, Kersters K, De Vos P, Logan NA, Ali N, Berkeley RC	Int J Syst Bacteriol	10.1099/00207713-46-1-270	1996	*
	Antimicrobial Activity from Putative Probiotic Lactic Acid Bacteria for the Biological Control of American and European Foulbrood Diseases.	Iorizzo M, Ganassi S, Albanese G, Letizia F, Testa B, Tedino C, Petrarca S, Mutinelli F, Mazzeo A, De Cristofaro A	Vet Sci	10.3390/vetsci9050236	2022	*
	Antimicrobial Activity against Paenibacillus larvae and Functional Properties of Lactiplantibacillus plantarum Strains: Potential Benefits for Honeybee Health.	Iorizzo M, Testa B, Lombardi SJ, Ganassi S, Ianiro M, Letizia F, Succi M, Tremonte P, Vergalito F, Cozzolino A, Sorrentino E, Coppola R, Petrarca S, Mancini M, De Cristofaro A	Antibiotics (Basel)	10.3390/antibiotics9080442	2020	*

References

#2955

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 7030

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#38668 ; Curators of the CIP;
#49263 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 28515

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#80820

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID1126.1 )

#120564 Collection of Institut Pasteur ; Curators of the CIP; CIP 104618
* These data were automatically processed and therefore are not curated

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Paenibacillus larvae subsp. larvae strain ERIC_I

Paenibacillus larvae subsp. larvae ATCC 9545

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