Paenibacillus macquariensis subsp. macquariensis (DSM 2, ATCC 23464, CIP 103269)

Name: Paenibacillus macquariensis subsp. macquariensis (DSM 2, ATCC 23464, CIP 103269)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 11477

Type strain

Culture col. no. DSM 2 ATCC 23464 CIP 103269 LMG 6935 NCTC 10419 2 more

NCBI tax ID(s) 948756 1468

Special Collections DSMZ CCUG CIP

History DSMZ <- ATCC <- B.J. Marshall CIP <- 1988, DSMZ, Bacillus macquariensis <- ATCC <- B.J. Marshall

Links

Paenibacillus macquariensis subsp. macquariensis DSM 2 is an aerobe, spore-forming, Gram-negative bacterium that was isolated from soil.

spore-forming Gram-negative rod-shaped aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 1151

LMG 6935,ATCC 23464,CECT 24,LMG 13289,NCIMB 9934,NCTC 10419,CCUG 37394,NCIB 9934,CIP 103269,KCTC 3538,NRRL B-14306,BCRC 15915,CCRC 15915,CCT 2468,KACC 10934,NCIM 2474,DSM 2

@ref 20215

Last LPSN update 2026-05-29 08:27:34

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Caryophanales

Family Paenibacillaceae

Genus Paenibacillus

Species Paenibacillus macquariensis subsp. macquariensis

Full scientific name Paenibacillus macquariensis subsp. macquariensis (Marshall and Ohye 1966) Hoshino et al. 2009

BacDive ID	Other strains from **Paenibacillus macquariensis subsp. macquariensis** (1)	Type strain
11476	P. macquariensis subsp. macquariensis DSM 489, ATCC 23465, LMG 13290, NCTC 10420

Morphology

Cell morphology

@ref	Gram stain	Cell shape
120740	negative	rod-shaped

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
37135	MEDIUM 3 - Columbia agar		Columbia agar (39.000 g);distilled water (1000.000 ml)
2010	NUTRIENT AGAR (DSMZ Medium 1)	Medium recipe at MediaDive	Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
120740	CIP Medium 3	Medium recipe at CIP
120740	CIP Medium 18	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
2010	positive	growth	20
37135	positive	growth	22
53646	positive	growth	20
53646	negative	growth	30
120740	positive	growth	10-22
120740	negative	growth	30
120740	negative	growth	37
120740	negative	growth	45
120740	negative	growth	55

Culture pH

@ref	Ability	Type	PH
120740	positive	growth	6

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
53646	aerobe
120740	facultative anaerobe
125439	facultative anaerobe	95.813

Spore formation

@ref	Spore formation	Confidence
125438		95.873
120740

Halophily

@ref	Salt	Growth	Tested relation	Concentration
120740	NaCl	positive	growth	0-2 %
120740	NaCl		growth	4 %
120740	NaCl		growth	6 %
120740	NaCl		growth	8 %
120740	NaCl		growth	10 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68371	27613 ChEBI	amygdalin	+	builds acid from	from API 50CH acid
68371	18305 ChEBI	arbutin	+	builds acid from	from API 50CH acid
68371	17057 ChEBI	cellobiose	+	builds acid from	from API 50CH acid
120740	16947 ChEBI	citrate	-	carbon source
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
68371	15824 ChEBI	D-fructose	+	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	12936 ChEBI	D-galactose	+	builds acid from	from API 50CH acid
68371	17634 ChEBI	D-glucose	+	builds acid from	from API 50CH acid
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68371	16899 ChEBI	D-mannitol	+	builds acid from	from API 50CH acid
68371	16024 ChEBI	D-mannose	+	builds acid from	from API 50CH acid
68371	16988 ChEBI	D-ribose	+	builds acid from	from API 50CH acid
68371	17924 ChEBI	D-sorbitol	-	builds acid from	from API 50CH acid
68371	16443 ChEBI	D-tagatose	-	builds acid from	from API 50CH acid
68371	65327 ChEBI	D-xylose	+	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
68371	4853 ChEBI	esculin	+	builds acid from	from API 50CH acid
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
68371	28066 ChEBI	gentiobiose	+	builds acid from	from API 50CH acid
68371	24265 ChEBI	gluconate	+	builds acid from	from API 50CH acid
68371	17754 ChEBI	glycerol	-	builds acid from	from API 50CH acid
68371	28087 ChEBI	glycogen	+	builds acid from	from API 50CH acid
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
68371	30849 ChEBI	L-arabinose	-	builds acid from	from API 50CH acid
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	+	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	-	builds acid from	from API 50CH acid
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
68371	17716 ChEBI	lactose	+	builds acid from	from API 50CH acid
68371	17306 ChEBI	maltose	+	builds acid from	from API 50CH acid
68371	6731 ChEBI	melezitose	+	builds acid from	from API 50CH acid
68371	28053 ChEBI	melibiose	+	builds acid from	from API 50CH acid
68371	320061 ChEBI	methyl alpha-D-glucopyranoside	+	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	+	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	+	builds acid from	from API 50CH acid
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
68371	59640 ChEBI	N-acetylglucosamine	+	builds acid from	from API 50CH acid
120740	17632 ChEBI	nitrate	-	reduction
120740	17632 ChEBI	nitrate	+	respiration
120740	16301 ChEBI	nitrite	-	reduction
68371	0 ChEBI	Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371	16634 ChEBI	raffinose	+	builds acid from	from API 50CH acid
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
68371	17814 ChEBI	salicin	+	builds acid from	from API 50CH acid
68371	28017 ChEBI	starch	+	builds acid from	from API 50CH acid
68371	17992 ChEBI	sucrose	+	builds acid from	from API 50CH acid
68371	27082 ChEBI	trehalose	+	builds acid from	from API 50CH acid
68371	32528 ChEBI	turanose	+	builds acid from	from API 50CH acid
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

Metabolite production

@ref	Chebi-ID	Metabolite	Production
120740	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test
120740	15688 ChEBI	acetoin	-

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
120740	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	+	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
120740	amylase	-
68382	beta-galactosidase	+	3.2.1.23	from API zym
68382	beta-glucosidase	+	3.2.1.21	from API zym
68382	beta-glucuronidase	+	3.2.1.31	from API zym
120740	caseinase	-	3.4.21.50
120740	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
120740	DNase	-
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
120740	gelatinase	-
120740	lecithinase	-
68382	leucine arylamidase	-	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
120740	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	-		from API zym
120740	ornithine decarboxylase	-	4.1.1.17
120740	oxidase	+
68382	trypsin	-	3.4.21.4	from API zym
120740	tween esterase	-
120740	urease	-	3.5.1.5
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
120740	-	+	+	+	-	-	-	-	-	-	+	-	+	+	+	+	+	-	-	-

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
120740	not determinedn.d.	-	-	-	-	+	+	-	-	+	+	+	+	+	-	-	-	-	+	-	+	+	+	+	+	+	+	+	+	+	+	+	+	-	+	+	+	+	-	+	+	-	-	-	+	-	-	+	-	+/-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Soil

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
2010	soil	Macquarie island	Australia	AUS	Australia and Oceania
53646	Soil
120740	Environment, Soil	Macquarie Island	Antarctica	ATA	Antarctica

Taxonmaps

69479

Global distribution of 16S sequence X60625 (>99% sequence identity) for Paenibacillus from Microbeatlas

Aquatic Samples	100
Soil Samples	1259
Animal Samples	152
Plant Samples	220
Total Samples	1731

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
2010	1	Risk group (German classification) According to TRBA 466
120740	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	Paenibacillus macquariensis strain ATCC 23464	contig		948756.4		948756	65
66792	IMG-taxon 2681812895 annotated assembly for Paenibacillus macquariensis ATCC 23464	scaffold	GCA_900156375	948756.3	2681812895	948756	63.62
124043	ASM163716v1 assembly for Paenibacillus macquariensis subsp. macquariensis DSM 2	contig	GCA_001637165	1468.3	2744054792	1468	62.71

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Paenibacillus macquariensis subsp. macquariensis genes for 16S rRNA, 16S-23S internal transcribed spacer, 23S rRNA, partial and complete sequence, strain: DSM2	AB468954	586	1468
20218	Paenibacillus macquariensis subsp. macquariensis partial 16S rRNA gene, strain DSM/ATCC23464	X57305	1556	1468
20218	Paenibacillus macquariensis subsp. macquariensis 16S ribosomal RNA, strain NCTC 10419	X60625	1438	1468
2010	Paenibacillus macquariensis subsp. macquariensis gene for 16S rRNA, partial sequence	AB073193	1539	1468

GC content

@ref	GC-content (mol%)	Method
2010	41.6	Buoyant density centrifugation (BD)
2010	39.3	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Paenibacillus macquariensis strain ATCC 23464
BV-BRC: 948756.4

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	facultative anaerobe	95.81	no
125439	gram_stain	BacteriaNetⓘ	variable	57.03	no
125439	motility	BacteriaNetⓘ	yes	65.94	no
125439	spore_formation	BacteriaNetⓘ	yes	75.61	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Paenibacillus macquariensis ATCC 23464
NCBI: GCA_900156375.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	56.46	no
125438	anaerobic	anaerobicⓘ	no	90.55	no
125438	aerobic	aerobicⓘ	yes	53.15	no
125438	spore-forming	spore-formingⓘ	yes	95.87	no
125438	thermophilic	thermophileⓘ	no	94.33	no
125438	flagellated	motile2+ⓘ	yes	86.03	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Genetics	Molecular characterization of Paenibacillus antarcticus IPAC21, a bioemulsifier producer isolated from Antarctic soil.	de Lemos EA, Procopio L, da Mota FF, Jurelevicius D, Rosado AS, Seldin L.	Front Microbiol	10.3389/fmicb.2023.1142582	2023
	Enhanced biodegradation of high-density polyethylene microplastics: Study of bacterial efficiency and process parameters.	Newrick BA, Valdes D, Laca A, Laca A, Diaz M.	J Hazard Mater	10.1016/j.jhazmat.2024.136822	2025
Phylogeny	Paenibacillus crassostreae sp. nov., isolated from the Pacific oyster Crassostrea gigas.	Shin SK, Kim E, Yi H	Int J Syst Evol Microbiol	10.1099/ijsem.0.002444	2017
Phylogeny	Paenibacillus glacialis sp. nov., isolated from the Kafni glacier of the Himalayas, India.	Kishore KH, Begum Z, Pathan AAK, Shivaji S	Int J Syst Evol Microbiol	10.1099/ijs.0.015271-0	2009
Phylogeny	Paenibacillus macquariensis subsp. defensor subsp. nov., isolated from boreal soil.	Hoshino T, Nakabayashi T, Hirota K, Matsuno T, Koiwa R, Fujiu S, Saito I, Tkachenko OB, Matsuyama H, Yumoto I	Int J Syst Evol Microbiol	10.1099/ijs.0.006304-0	2009
Phylogeny	Paenibacillus antarcticus sp. nov., a novel psychrotolerant organism from the Antarctic environment.	Montes MJ, Mercade E, Bozal N, Guinea J	Int J Syst Evol Microbiol	10.1099/ijs.0.63078-0	2004

References

#2010	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 2
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#37135	; Curators of the CIP;
#53646	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 37394
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#68371	Automatically annotated from API 50CH acid .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#120740	Collection of Institut Pasteur ; Curators of the CIP; CIP 103269
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Paenibacillus macquariensis subsp. macquariensis (DSM 2, ATCC 23464, CIP 103269)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API zym

API 50CHac

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_900156375

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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