Name: Paenibacillus macquariensis DSM 2
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 11477

Type strain:

Species: Paenibacillus macquariensis

Culture col. no.: DSM 2, ATCC 23464, CIP 103269, LMG 6935, NCTC 10419, CCUG 37394, NCIMB 9934

Strain history: CIP <- 1988, DSMZ, Bacillus macquariensis <- ATCC <- B.J. Marshall

NCBI tax ID(s): 948756 (species)

SI-ID 1151

LMG 6935, ATCC 23464, CECT 24, LMG 13289, NCIMB 9934, NCTC 10419, CCUG 37394, NCIB 9934, CIP 103269, KCTC 3538, NRRL B-14306, BCRC 15915, CCRC 15915, CCT 2468, KACC 10934, NCIM 2474, DSM 2

Section

Paenibacillus macquariensis DSM 2 is an aerobe, spore-forming, psychrophilic bacterium that was isolated from soil.

motile
spore-forming
aerobe
psychrophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Paenibacillaceae*
	Genus	*Paenibacillus*
	Species	**Paenibacillus macquariensis**
	Full Scientific Name (LPSN)	Paenibacillus macquariensis (Marshall and Ohye 1966) Ash et al. 1994

Synonym

Bacillus macquariensis

Information on morphological and physiological properties Morphology

[Ref.: #120740]	Gram stain	negative
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #120740]	Cell shape	rod-shaped

[Ref.: #69480]	Motility	yes Confidence in %95.234

Information on culture and growth conditions Culture and growth conditions

[Ref.: #37135]

Culture medium

MEDIUM 3 - Columbia agar

[Ref.: #37135]

Culture medium growth

[Ref.: #37135]

Culture medium composition

expand / minimize

[Ref.: #2010]

Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

[Ref.: #2010]

Culture medium growth

[Ref.: #2010]

Culture medium link

Medium recipe at MediaDive

[Ref.: #2010]

Culture medium composition

expand / minimize

[Ref.: #120740]

Culture medium

CIP Medium 3

[Ref.: #120740]

Culture medium growth

[Ref.: #120740]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #120740]

Culture medium

CIP Medium 18

[Ref.: #120740]

Culture medium growth

[Ref.: #120740]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #2010]		growth	20 °C
[Ref.: #37135]		growth	22 °C
[Ref.: #53646]		growth	20 °C
[Ref.: #53646]	no	growth	30 °C
[Ref.: #120740]		growth	10-22 °C
[Ref.: #120740]	no	growth	30 °C
[Ref.: #120740]	no	growth	37 °C
[Ref.: #120740]	no	growth	45 °C
[Ref.: #120740]	no	growth	55 °C

[Ref.: #2010]	Temperature range	psychrophilic
[Ref.: #37135]	Temperature range	psychrophilic
[Ref.: #53646]	Temperature range	psychrophilic
[Ref.: #53646]	Temperature range	mesophilic
[Ref.: #120740]	Temperature range	psychrophilic
[Ref.: #120740]	Temperature range	mesophilic
[Ref.: #120740]	Temperature range	thermophilic

	pH	Kind of pH		pH
[Ref.: #120740]			growth	6

Information on physiology and metabolism Physiology and metabolism

[Ref.: #53646]	Oxygen tolerance	aerobe
[Ref.: #120740]	Oxygen tolerance	facultative anaerobe

[Ref.: #120740]	Ability of spore formation	yes
[Ref.: #69481]	Ability of spore formation	yes Confidence in %100
[Ref.: #69480]	Ability of spore formation	yes Confidence in %100

	Salt	Tested relation		Salt conc.
[Ref.: #120740]	NaCl		growth	0-2	%
[Ref.: #120740]	NaCl	no	growth	4	%
[Ref.: #120740]	NaCl	no	growth	6	%
[Ref.: #120740]	NaCl	no	growth	8	%
[Ref.: #120740]	NaCl	no	growth	10	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68371]	amygdalin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	arbutin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	cellobiose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #120740]	citrate ChEBI	-	carbon source
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fructose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-galactose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-glucose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-ribose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-xylose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	esculin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gentiobiose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	gluconate ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycerol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycogen ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	lactose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	maltose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	melezitose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	melibiose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-glucopyranoside ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	N-acetylglucosamine ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #120740]	nitrate ChEBI	-	reduction
[Ref.: #120740]	nitrate ChEBI	+	respiration
[Ref.: #120740]	nitrite ChEBI	-	reduction
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	raffinose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	salicin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	starch ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	sucrose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	trehalose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	turanose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #120740]		indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #120740]		acetoin ChEBI	-

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #120740]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	+	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #120740]	amylase	-
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	+	3.2.1.31	* from API zym
[Ref.: #120740]	caseinase	-	3.4.21.50
[Ref.: #120740]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #120740]	DNase	-
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #120740]	gelatinase	-
[Ref.: #120740]	lecithinase	-
[Ref.: #68382]	leucine arylamidase	-	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #120740]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #120740]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #120740]	oxidase	+
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #120740]	tween esterase	-
[Ref.: #120740]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #120740]	9235	not determinedn.d.	-	-	-	-	+	+	-	-	+	+	+	+	+	-	-	-	-	+	-	+	+	+	+	+	+	+	+	+	+	+	+	+	-	+	+	+	+	-	+	+	-	-	-	+	-	-	+	-	+/-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #120740]	7473	-	+	+	+	-	-	-	-	-	-	+	-	+	+	+	+	+	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #2010]	Sample type/isolated from	soil
[Ref.: #2010]	Geographic location (country and/or sea, region)	Macquarie island
[Ref.: #2010]	Country	Australia
[Ref.: #2010]	Country ISO 3 Code	AUS
[Ref.: #2010]	Continent	Australia and Oceania

[Ref.: #53646]	Sample type/isolated from	Soil

[Ref.: #120740]	Sample type/isolated from	Environment, Soil
[Ref.: #120740]	Geographic location (country and/or sea, region)	Macquarie Island
[Ref.: #120740]	Country	Antarctica
[Ref.: #120740]	Country ISO 3 Code	ATA
[Ref.: #120740]	Continent	Antarctica
* marker position based on {}

Isolation sources categories

#Environmental

#Terrestrial

#Soil

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence X60625 (>99% sequence identity) for Paenibacillus from Microbeatlas

Aquatic Samples	100
Soil Samples	1259
Animal Samples	152
Plant Samples	220
Total Samples	1731

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #2010]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #120740]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #2010]	Paenibacillus macquariensis subsp. macquariensis gene for 16S rRNA, partial sequence	AB073193	1539	ENA	1468 tax ID
[Ref.: #20218]	Vibrionaceae bacterium DSM2 16S ribosomal RNA gene, partial sequence	KC439178	925	ENA	1343286 tax ID	*
[Ref.: #20218]	Paenibacillus macquariensis subsp. macquariensis partial 16S rRNA gene, strain DSM/ATCC23464	X57305	1556	ENA	1468 tax ID	*
[Ref.: #20218]	Paenibacillus macquariensis subsp. macquariensis genes for 16S rRNA, 16S-23S internal transcribed spacer, 23S rRNA, partial and complete sequence, strain: DSM2	AB468954	586	ENA	1468 tax ID	*
[Ref.: #20218]	Paenibacillus macquariensis subsp. macquariensis 16S ribosomal RNA, strain NCTC 10419	X60625	1438	ENA	1468 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Paenibacillus macquariensis ATCC 23464	GCA_900156375	scaffold	GenBank	948756 tax ID	*
[Ref.: #66792]	Paenibacillus macquariensis strain ATCC 23464	948756.4	wgs	PATRIC	948756 tax ID	*
[Ref.: #66792]	Paenibacillus macquariensis strain ATCC 23464	948756.3	wgs	PATRIC	948756 tax ID	*
[Ref.: #66792]	Paenibacillus macquariensis ATCC 23464	2681812895	draft	JGI IMG/M	948756 tax ID	*

[Ref.: #2010]	GC-content	41.6 mol%	Buoyant density centrifugation (BD)
[Ref.: #2010]	GC-content	39.3 mol%	thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Paenibacillus macquariensis ATCC 23464 NCBI: GCA_900156375.1
[Ref.: #69481]	spore-forming	yes	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Paenibacillus macquariensis ATCC 23464 NCBI: GCA_900156375.1
[Ref.: #69480]	motile	yes	90.614	no
[Ref.: #69480]	flagellated	yes	86.627	no
[Ref.: #69480]	gram-positive	yes	68.468	no
[Ref.: #69480]	anaerobic	no	97.138	yes
[Ref.: #69480]	aerobic	yes	72.371	no
[Ref.: #69480]	halophile	no	93.589	no
[Ref.: #69480]	spore-forming	yes	96.449	no
[Ref.: #69480]	thermophile	no	99.942	yes
[Ref.: #69480]	glucose-util	yes	88.024	no
[Ref.: #69480]	glucose-ferment	no	79.174	no

Availability in culture collections External links

[Ref.: #2010]

Culture collection no.

DSM 2, ATCC 23464, CIP 103269, LMG 6935, NCTC 10419, CCUG 37394, NCIMB 9934

[Ref.: #80711]

SI-ID 1151

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Paenibacillus crassostreae sp. nov., isolated from the Pacific oyster Crassostrea gigas.	Shin SK, Kim E, Yi H	Int J Syst Evol Microbiol	10.1099/ijsem.0.002444	2017	*
Phylogeny	Paenibacillus glacialis sp. nov., isolated from the Kafni glacier of the Himalayas, India.	Kishore KH, Begum Z, Pathan AAK, Shivaji S	Int J Syst Evol Microbiol	10.1099/ijs.0.015271-0	2009	*
Phylogeny	Paenibacillus macquariensis subsp. defensor subsp. nov., isolated from boreal soil.	Hoshino T, Nakabayashi T, Hirota K, Matsuno T, Koiwa R, Fujiu S, Saito I, Tkachenko OB, Matsuyama H, Yumoto I	Int J Syst Evol Microbiol	10.1099/ijs.0.006304-0	2009	*
Phylogeny	Paenibacillus antarcticus sp. nov., a novel psychrotolerant organism from the Antarctic environment.	Montes MJ, Mercade E, Bozal N, Guinea J	Int J Syst Evol Microbiol	10.1099/ijs.0.63078-0	2004	*

References

#2010

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 2

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#37135 ; Curators of the CIP;
#53646 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 37394

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68371

Automatically annotated from API 50CH acid .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#80711

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID1151.1 )

#120740 Collection of Institut Pasteur ; Curators of the CIP; CIP 103269
* These data were automatically processed and therefore are not curated

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Paenibacillus macquariensis ATCC 23464

Paenibacillus macquariensis ATCC 23464

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