Name: Cohnella xylanilytica MX15-2
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 11453

Type strain:

Species: Cohnella xylanilytica

Strain Designation: MX15-2

Culture col. no.: DSM 25239, KCTC 22294, PCU 309, TISTR 1891

Strain history: <- S Tanasupawat, Chulalongkorn Univ., Thailand

NCBI tax ID(s): 557555 (species)

SI-ID 405184

DSM 25239

Section

Cohnella xylanilytica MX15-2 is a facultative anaerobe, spore-forming, mesophilic bacterium that was isolated from soil.

Gram-positive
motile
rod-shaped
spore-forming
facultative anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Paenibacillaceae*
	Genus	*Cohnella*
	Species	**Cohnella xylanilytica**
	Full Scientific Name (LPSN)	Cohnella xylanilytica Khianngam et al. 2010

Information on morphological and physiological properties Morphology

[Ref.: #29648]	Gram stain	positive
[Ref.: #67771]	Gram stain	positive

[Ref.: #29648]	Cell length	1.4-3.5 µm

[Ref.: #29648]	Cell width	0.3-0.5 µm

[Ref.: #29648]	Cell shape	rod-shaped
[Ref.: #67771]	Cell shape	rod-shaped

[Ref.: #29648]	Motility	yes
[Ref.: #67771]	Motility	yes

[Ref.: #67771]	Flagellum arrangement	peritrichous

[Ref.: #29648]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #17953]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #17953]

Culture medium growth

[Ref.: #17953]

Culture medium link

Medium recipe at MediaDive

[Ref.: #17953]

Culture medium composition

expand / minimize

[Ref.: #17953]

Culture medium

CASO AGAR (MERCK 105458) (DSMZ Medium 220)

[Ref.: #17953]

Culture medium growth

[Ref.: #17953]

Culture medium link

Medium recipe at MediaDive

[Ref.: #17953]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #17953]	growth	37 °C
[Ref.: #29648]	growth	20-50 °C
[Ref.: #29648]	optimum	30 °C
[Ref.: #67771]	growth	30-40 °C

[Ref.: #17953]	Temperature range	mesophilic
[Ref.: #29648]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #29648]			optimum	7
[Ref.: #29648]			growth	06-09

Information on physiology and metabolism Physiology and metabolism

[Ref.: #29648]	Oxygen tolerance	facultative anaerobe
[Ref.: #67771]	Oxygen tolerance	facultative anaerobe

[Ref.: #67771]	Type of spore	endospore
[Ref.: #67771]	Ability of spore formation	yes
[Ref.: #29648]	Ability of spore formation	yes

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #29648]		NaCl		growth	3	%
[Ref.: #29648]		NaCl		optimum	3	%

[Ref.: #29648]	Observation	aggregates in chains
[Ref.: #67771]	Observation	quinones: MK-7

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #29648]	arabinose ChEBI	+	carbon source
[Ref.: #29648]	esculin ChEBI	+	hydrolysis
[Ref.: #29648]	glucose ChEBI	+	carbon source
[Ref.: #29648]	malate ChEBI	+	carbon source
[Ref.: #29648]	maltose ChEBI	+	carbon source
[Ref.: #29648]	mannitol ChEBI	+	carbon source
[Ref.: #29648]	mannose ChEBI	+	carbon source

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #29648]		catalase	+	1.11.1.6
[Ref.: #29648]		cytochrome oxidase	+	1.9.3.1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #17953]	Sample type/isolated from	soil
[Ref.: #17953]	Geographic location (country and/or sea, region)	Nan Province
[Ref.: #17953]	Country	Thailand
[Ref.: #17953]	Country ISO 3 Code	THA
[Ref.: #17953]	Continent	Asia

[Ref.: #67771]	Sample type/isolated from	From soil
[Ref.: #67771]	Geographic location (country and/or sea, region)	Nan Province
[Ref.: #67771]	Country	Thailand
[Ref.: #67771]	Country ISO 3 Code	THA
[Ref.: #67771]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental

#Terrestrial

#Soil

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence HE866503 (>99% sequence identity) for Cohnella xylanilytica subclade from Microbeatlas

Aquatic Samples	12
Soil Samples	104
Animal Samples	13
Plant Samples	15
Total Samples	144

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #17953]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #17953]	Cohnella xylanilytica partial 16S rRNA gene, clone K26	HE866501	1604	ENA	557555 tax ID
[Ref.: #17953]	Cohnella xylanilytica partial 16S rRNA gene, clone K25	HE866502	1540	ENA	557555 tax ID
[Ref.: #17953]	Cohnella xylanilytica partial 16S rRNA gene, clone K24	HE866503	1540	ENA	557555 tax ID
[Ref.: #17953]	Cohnella xylanilytica partial 16S rRNA gene, clone K27	HE866500	1539	ENA	557555 tax ID
[Ref.: #29648]	Cohnella xylanilytica strain MX15-2 16S ribosomal RNA gene, partial sequence	FJ001841	1507	GenBank	557555 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Cohnella xylanilytica DSM 25239	GCA_014212175	scaffold	GenBank	557555 tax ID	*
[Ref.: #66792]	Cohnella xylanilytica strain DSM 25239	557555.3	wgs	PATRIC	557555 tax ID	*

[Ref.: #29648]	GC-content	63 mol%
[Ref.: #67771]	GC-content	63.0 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Cohnella xylanilytica DSM 25239 NCBI: GCA_014212175.1
[Ref.: #69480]	flagellated	yes	83.188	no
[Ref.: #69480]	gram-positive	yes	85.217	yes
[Ref.: #69480]	anaerobic	no	98.584	yes
[Ref.: #69480]	aerobic	yes	82.847	no
[Ref.: #69480]	halophile	no	97.042	no
[Ref.: #69480]	spore-forming	yes	96.038	no
[Ref.: #69480]	glucose-util	yes	89.875	no
[Ref.: #69480]	thermophile	no	99.57	no
[Ref.: #69480]	motile	yes	88.326	yes
[Ref.: #69480]	glucose-ferment	no	89.628	no

Availability in culture collections External links

[Ref.: #17953]

Culture collection no.

DSM 25239, KCTC 22294, PCU 309, TISTR 1891

[Ref.: #80691]

SI-ID 405184

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Cohnella xylanilytica sp. nov. and Cohnella terrae sp. nov., xylanolytic bacteria from soil.	Khianngam S, Tanasupawat S, Akaracharanya A, Kim KK, Lee KC, Lee JS	Int J Syst Evol Microbiol	10.1099/ijs.0.017855-0	2010	*

References

#17953

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 25239

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#29648

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26037 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#80691

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID405184.1 )

#26037

IJSEM 2913 2010 ( DOI 10.1099/ijs.0.017855-0 , PubMed 20097800 )

* These data were automatically processed and therefore are not curated

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Cohnella xylanilytica DSM 25239

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