Cohnella thermotolerans | Type strain | DSM 17683, CCUG 47242, CIP 108492 | BacDiveID:11442

Strain identifier

BacDive ID: 11442

Type strain:

Species: Cohnella thermotolerans

Culture col. no.: DSM 17683, CCUG 47242, CIP 108492

Strain history: CIP <- 2004, CCUG <- S. Karlstad, Stora Enso, Makhoul L

NCBI tax ID(s): 1121347 (strain), 329858 (species)

SI-ID 110965

CCUG 47242, CIP 108492, DSM 17683

Section

Cohnella thermotolerans DSM 17683 is an aerobe, spore-forming, mesophilic bacterium that was isolated from sample of industrial starch production.

Gram-positive
rod-shaped
spore-forming
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Paenibacillaceae*
	Genus	*Cohnella*
	Species	**Cohnella thermotolerans**
	Full Scientific Name (LPSN)	Cohnella thermotolerans Kämpfer et al. 2006

Information on morphological and physiological properties Morphology

[Ref.: #31660]	Gram stain	positive
[Ref.: #123517]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #31660]	Cell shape	rod-shaped
[Ref.: #123517]	Cell shape	coccus-shaped

[Ref.: #31660]	Motility	no
[Ref.: #69480]	Motility	yes Confidence in %96.955

Pigment production

yes

Multimedia content

Caption:

electron microscopic image

Intellectual property rights:

© HZI/Manfred Rohde

Caption:

electron microscopic image

Intellectual property rights:

© HZI/Manfred Rohde

Caption:

electron microscopic image

Intellectual property rights:

© HZI/Manfred Rohde

Caption:

electron microscopic image

Intellectual property rights:

© HZI/Manfred Rohde

Caption:

electron microscopic image

Intellectual property rights:

© HZI/Manfred Rohde

Information on culture and growth conditions Culture and growth conditions

Culture medium

CASO AGAR (MERCK 105458) (DSMZ Medium 220)

Culture medium growth

Culture medium link

Medium recipe at MediaDive

Culture medium composition

expand / minimize

Culture medium

MEDIUM 29- Brain heart agar

Culture medium growth

Culture medium composition

expand / minimize

[Ref.: #123517]

Culture medium

CIP Medium 72

[Ref.: #123517]

Culture medium growth

[Ref.: #123517]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #123517]

Culture medium

CIP Medium 29

[Ref.: #123517]

Culture medium growth

[Ref.: #123517]

Culture medium link

Medium recipe provided by DSMZ

	Temperatures	Kind of temperature		Temperature
[Ref.: #7125]			growth	30 °C
[Ref.: #31660]			growth	25-55 °C
[Ref.: #31660]			optimum	25-30 °C
[Ref.: #36517]			growth	37 °C
[Ref.: #57600]			growth	37 °C
[Ref.: #123517]			growth	30-55 °C
[Ref.: #123517]		no	growth	10 °C
[Ref.: #123517]		no	growth	22 °C

[Ref.: #7125]	Temperature range	mesophilic
[Ref.: #31660]	Temperature range	mesophilic
[Ref.: #36517]	Temperature range	mesophilic
[Ref.: #57600]	Temperature range	mesophilic
[Ref.: #123517]	Temperature range	psychrophilic

	pH	Kind of pH		pH
[Ref.: #31660]			optimum	7
[Ref.: #31660]			growth	7
[Ref.: #123517]			growth	6

Information on physiology and metabolism Physiology and metabolism

Oxygen tolerance

aerobe

[Ref.: #31660]	Ability of spore formation	yes
[Ref.: #123517]	Ability of spore formation	yes
[Ref.: #69481]	Ability of spore formation	yes Confidence in %100
[Ref.: #69480]	Ability of spore formation	yes Confidence in %100

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #123517]		NaCl		growth	0	%
[Ref.: #123517]		NaCl	no	growth	2	%
[Ref.: #123517]		NaCl	no	growth	4	%
[Ref.: #123517]		NaCl	no	growth	6	%
[Ref.: #123517]		NaCl	no	growth	8	%
[Ref.: #123517]		NaCl	no	growth	10	%

Observation

aggregates in chains

	Metabolite utilization	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #31660]		arabinose ChEBI	+	carbon source
[Ref.: #31660]		cellobiose ChEBI	+	carbon source
[Ref.: #123517]		citrate ChEBI	-	carbon source
[Ref.: #31660]		esculin ChEBI	+	hydrolysis
[Ref.: #123517]		esculin ChEBI	+	hydrolysis
[Ref.: #31660]		fructose ChEBI	+	carbon source
[Ref.: #31660]		galactose ChEBI	+	carbon source
[Ref.: #31660]		gluconate ChEBI	+	carbon source
[Ref.: #31660]		glucose ChEBI	+	carbon source
[Ref.: #123517]		hippurate ChEBI	+	hydrolysis
[Ref.: #31660]		maltose ChEBI	+	carbon source
[Ref.: #31660]		mannose ChEBI	+	carbon source
[Ref.: #31660]		melibiose ChEBI	+	carbon source
[Ref.: #123517]		nitrate ChEBI	-	reduction
[Ref.: #123517]		nitrate ChEBI	-	respiration
[Ref.: #123517]		nitrite ChEBI	-	reduction
[Ref.: #31660]		rhamnose ChEBI	+	carbon source
[Ref.: #31660]		ribose ChEBI	+	carbon source
		Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #123517]		indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test	Methylred-test
[Ref.: #123517]		acetoin ChEBI	-
[Ref.: #123517]		glucose ChEBI		-

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #68382]		acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #123517]		alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]		alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]		alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]		alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]		alpha-galactosidase	+	3.2.1.22	* from API zym
[Ref.: #68382]		alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]		alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #123517]		amylase	+
[Ref.: #123517]		beta-galactosidase	+	3.2.1.23
[Ref.: #68382]		beta-galactosidase	+	3.2.1.23	* from API zym
[Ref.: #68382]		beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]		beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #123517]		caseinase	-	3.4.21.50
[Ref.: #123517]		catalase	+	1.11.1.6
[Ref.: #68382]		cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #31660]		cytochrome oxidase	+	1.9.3.1
[Ref.: #123517]		DNase	-
[Ref.: #68382]		esterase (C 4)	+		* from API zym
[Ref.: #68382]		esterase lipase (C 8)	-		* from API zym
[Ref.: #123517]		gamma-glutamyltransferase	+	2.3.2.2
[Ref.: #123517]		gelatinase	-
[Ref.: #123517]		lecithinase	-
[Ref.: #68382]		leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #123517]		lipase	-
[Ref.: #68382]		lipase (C 14)	-		* from API zym
[Ref.: #123517]		lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]		N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API zym
[Ref.: #68382]		naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #123517]		ornithine decarboxylase	-	4.1.1.17
[Ref.: #123517]		oxidase	-
[Ref.: #123517]		protease	-
[Ref.: #68382]		trypsin	-	3.4.21.4	* from API zym
[Ref.: #123517]		tryptophan deaminase	-
[Ref.: #123517]		tween esterase	-
[Ref.: #123517]		urease	-	3.5.1.5
[Ref.: #68382]		valine arylamidase	-		* from API zym
		Only first 10 entries are displayed. Click here to see all.

API® Biotype100:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
[Ref.: #123517]	2772	not determinedn.d.	+	+	+	+	+	-	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	-	+	-	+	-	-	+	+	+	+	+	+	-	-	+	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	+	+	+	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #123517]	11224	-	-	+	-	-	+	-	-	-	-	+	+	+	+	-	+	+	+	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #7125]	Sample type/isolated from	sample of industrial starch production
[Ref.: #7125]	Country	Sweden
[Ref.: #7125]	Country ISO 3 Code	SWE
[Ref.: #7125]	Continent	Europe

[Ref.: #57600]	Sample type/isolated from	Industrial starch
[Ref.: #57600]	Sampling date	2002-12-01
[Ref.: #57600]	Country	Sweden
[Ref.: #57600]	Country ISO 3 Code	SWE
[Ref.: #57600]	Continent	Europe

[Ref.: #123517]	Sample type/isolated from	Environment, Industrial starch
[Ref.: #123517]	Country	Sweden
[Ref.: #123517]	Country ISO 3 Code	SWE
[Ref.: #123517]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Engineered	#Food production	#Food
#Engineered	#Industrial	-

What are isolation sources categories?

Global distribution of 16S sequence AJ971483 (>99% sequence identity) for Cohnella thermotolerans subclade from Microbeatlas

Aquatic Samples	49
Soil Samples	248
Animal Samples	119
Plant Samples	147
Total Samples	563

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #7125]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #123517]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #7125]	Cohnia thermotolerans partial 16S rRNA gene, type strain CCUG 47242T	AJ971483	1486	ENA	329858 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Cohnella thermotolerans DSM 17683	GCA_000429825	scaffold	GenBank	1121347 tax ID	*
[Ref.: #66792]	Cohnella thermotolerans DSM 17683	1121347.4	wgs	PATRIC	1121347 tax ID	*
[Ref.: #66792]	Cohnella thermotolerans DSM 17683	2524023169	draft	JGI IMG/M	1121347 tax ID	*

GC-content

59 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Cohnella thermotolerans DSM 17683 DSM 17683 NCBI: GCA_000429825.1
[Ref.: #69481]	spore-forming	yes	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Cohnella thermotolerans DSM 17683 DSM 17683 NCBI: GCA_000429825.1
[Ref.: #69480]	motile	yes	84.192	yes
[Ref.: #69480]	flagellated	yes	79.253	no
[Ref.: #69480]	gram-positive	yes	87.614	yes
[Ref.: #69480]	anaerobic	no	98.625	yes
[Ref.: #69480]	aerobic	yes	89.512	yes
[Ref.: #69480]	halophile	no	96.66	no
[Ref.: #69480]	spore-forming	yes	95.909	yes
[Ref.: #69480]	thermophile	no	93.571	no
[Ref.: #69480]	glucose-util	yes	87.889	yes
[Ref.: #69480]	glucose-ferment	no	91.554	no

Availability in culture collections External links

Culture collection no.

DSM 17683, CCUG 47242, CIP 108492

SI-ID 110965

*

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Cohnella terricola sp. nov., isolated from soil.	Kim J, Chhetri G, Kim I, Kang M, Seo T	Int J Syst Evol Microbiol	10.1099/ijsem.0.004835	2021	*
Phylogeny	Cohnella nanjingensis sp. nov., an extracellular polysaccharide-producing bacterium isolated from soil.	Huang Z, Yu YJ, Bao YY, Xia L, Sheng XF, He LY	Int J Syst Evol Microbiol	10.1099/ijs.0.066456-0	2014	*
Phylogeny	Cohnella formosensis sp. nov., a xylanolytic bacterium isolated from the rhizosphere of Medicago sativa L.	Hameed A, Hung MH, Lin SY, Hsu YH, Liu YC, Shahina M, Lai WA, Huang HC, Young LS, Young CC	Int J Syst Evol Microbiol	10.1099/ijs.0.045831-0	2013	*
Phylogeny	Cohnella xylanilytica sp. nov. and Cohnella terrae sp. nov., xylanolytic bacteria from soil.	Khianngam S, Tanasupawat S, Akaracharanya A, Kim KK, Lee KC, Lee JS	Int J Syst Evol Microbiol	10.1099/ijs.0.017855-0	2010	*
Phylogeny	Cohnella thailandensis sp. nov., a xylanolytic bacterium from Thai soil.	Khianngam S, Tanasupawat S, Akaracharanya A, Kim KK, Lee KC, Lee JS	Int J Syst Evol Microbiol	10.1099/ijs.0.015859-0	2009	*
Phylogeny	Cohnella yongneupensis sp. nov. and Cohnella ginsengisoli sp. nov., isolated from two different soils.	Kim SJ, Weon HY, Kim YS, Anandham R, Jeon YA, Hong SB, Kwon SW	Int J Syst Evol Microbiol	10.1099/ijs.0.013581-0	2009	*
Phylogeny	Cohnella panacarvi sp. nov., a xylanolytic bacterium isolated from ginseng cultivating soil.	Yoon MH, Ten LN, Im WT	J Microbiol Biotechnol		2007	*
Phylogeny	Cohnella thermotolerans gen. nov., sp. nov., and classification of 'Paenibacillus hongkongensis' as Cohnella hongkongensis sp. nov.	Kampfer P, Rossello-Mora R, Falsen E, Busse HJ, Tindall BJ	Int J Syst Evol Microbiol	10.1099/ijs.0.63985-0	2006	*

References

#7125

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 17683

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#31660

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27941 (see below)

#36517 ; Curators of the CIP;
#57600 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 47242

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#66793

Mukherjee et al.: GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life. 35: 676 - 683 2017 ( DOI 10.1038/nbt.3886 , PubMed 28604660 )

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#80680

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID110965.1 )

#123517 Collection of Institut Pasteur ; Curators of the CIP; CIP 108492

#27941

IJSEM 781 2006 ( DOI 10.1099/ijs.0.63985-0 , PubMed 16585694 )

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.