Aneurinibacillus migulanus (DSM 5759, NRS 1138, B1)

Name: Aneurinibacillus migulanus (DSM 5759, NRS 1138, B1)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 11383

Type strain

Strain Designation B1

Culture col. no. DSM 5759 NRS 1138 B1

NCBI tax ID(s) 47500

Special Collections DSMZ

History DSMZ <- NRRL; NRS 1138 <- C.P. Hegarty, Sharp and Dohme, Inc., 1946 (Bacillus brevis) gramicidin S strain B1 <- S.A. Waksman <- Inst. of Tropical Medicine, Moscow

Links

Aneurinibacillus migulanus B1 is a bacterium of the family Paenibacillaceae.

genome sequence Bacteria

Name and taxonomic classification

SI-ID 297065

DSM 5759

@ref 20215

Last LPSN update 2026-05-29 08:27:33

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Caryophanales

Family Paenibacillaceae

Genus Aneurinibacillus

Species Aneurinibacillus migulanus

Full scientific name Aneurinibacillus migulanus (Takagi et al. 1993) Shida et al. 1996

Synonyms (1)

Bacillus migulanus

BacDive ID	Other strains from **Aneurinibacillus migulanus** (3)	Type strain
11381	A. migulanus DSM 2895, ATCC 9999, CIP 103841, IFO 15520, ... (type strain)
11382	A. migulanus B2, DSM 5668, NRS 1139
11384	A. migulanus DSM 8373, NRRL NRS-606

Morphology

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
2317	NUTRIENT AGAR (DSMZ Medium 1)		Medium recipe at MediaDive	Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
2317	positive	growth	30

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
125439	obligate aerobe	91.185

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	adipate degradation	100	2 of 2
66794	C4 and CAM-carbon fixation	100	8 of 8
66794	glycine betaine biosynthesis	100	5 of 5
66794	formaldehyde oxidation	100	3 of 3
66794	ppGpp biosynthesis	100	4 of 4
66794	lipoate biosynthesis	100	5 of 5
66794	ribulose monophosphate pathway	100	2 of 2
66794	molybdenum cofactor biosynthesis	100	9 of 9
66794	palmitate biosynthesis	100	22 of 22
66794	phenylacetate degradation (aerobic)	100	5 of 5
66794	acetate fermentation	100	4 of 4
66794	aspartate and asparagine metabolism	100	9 of 9
66794	pentose phosphate pathway	100	11 of 11
66794	ethanol fermentation	100	2 of 2
66794	gluconeogenesis	100	8 of 8
66794	coenzyme A metabolism	100	4 of 4
66794	biotin biosynthesis	100	4 of 4
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	folate polyglutamylation	100	1 of 1
66794	aerobactin biosynthesis	100	1 of 1
66794	methylglyoxal degradation	100	5 of 5
66794	L-lactaldehyde degradation	100	3 of 3
66794	ceramide biosynthesis	100	1 of 1
66794	suberin monomers biosynthesis	100	2 of 2
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	propanol degradation	100	7 of 7
66794	sulfopterin metabolism	100	4 of 4
66794	cis-vaccenate biosynthesis	100	2 of 2
66794	tetrahydrofolate metabolism	92.86	13 of 14
66794	phenylalanine metabolism	92.31	12 of 13
66794	vitamin B1 metabolism	92.31	12 of 13
66794	proline metabolism	90.91	10 of 11
66794	4-hydroxyphenylacetate degradation	90	9 of 10
66794	threonine metabolism	90	9 of 10
66794	propionate fermentation	90	9 of 10
66794	alanine metabolism	89.66	26 of 29
66794	glutamate and glutamine metabolism	89.29	25 of 28
66794	serine metabolism	88.89	8 of 9
66794	valine metabolism	88.89	8 of 9
66794	CO2 fixation in Crenarchaeota	88.89	8 of 9
66794	chorismate metabolism	88.89	8 of 9
66794	ketogluconate metabolism	87.5	7 of 8
66794	flavin biosynthesis	86.67	13 of 15
66794	reductive acetyl coenzyme A pathway	85.71	6 of 7
66794	vitamin B12 metabolism	85.29	29 of 34
66794	phenol degradation	85	17 of 20
66794	methionine metabolism	84.62	22 of 26
66794	leucine metabolism	84.62	11 of 13
66794	NAD metabolism	83.33	15 of 18
66794	1,4-dihydroxy-6-naphthoate biosynthesis	83.33	5 of 6
66794	pyrimidine metabolism	82.22	37 of 45
66794	Entner Doudoroff pathway	80	8 of 10
66794	factor 420 biosynthesis	80	4 of 5
66794	cellulose degradation	80	4 of 5
66794	peptidoglycan biosynthesis	80	12 of 15
66794	metabolism of amino sugars and derivatives	80	4 of 5
66794	tryptophan metabolism	78.95	30 of 38
66794	heme metabolism	78.57	11 of 14
66794	photosynthesis	78.57	11 of 14
66794	citric acid cycle	78.57	11 of 14
66794	d-mannose degradation	77.78	7 of 9
66794	purine metabolism	77.66	73 of 94
66794	urea cycle	76.92	10 of 13
66794	butanoate fermentation	75	3 of 4
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	75	6 of 8
66794	isoleucine metabolism	75	6 of 8
66794	cardiolipin biosynthesis	71.43	5 of 7
66794	ubiquinone biosynthesis	71.43	5 of 7
66794	glutathione metabolism	71.43	10 of 14
66794	glycolysis	70.59	12 of 17
66794	myo-inositol biosynthesis	70	7 of 10
66794	coenzyme M biosynthesis	70	7 of 10
66794	histidine metabolism	68.97	20 of 29
66794	non-pathway related	68.42	26 of 38
66794	oxidative phosphorylation	68.13	62 of 91
66794	acetyl CoA biosynthesis	66.67	2 of 3
66794	acetoin degradation	66.67	2 of 3
66794	octane oxidation	66.67	2 of 3
66794	enterobactin biosynthesis	66.67	2 of 3
66794	methane metabolism	66.67	2 of 3
66794	glycolate and glyoxylate degradation	66.67	4 of 6
66794	cyanate degradation	66.67	2 of 3
66794	cysteine metabolism	66.67	12 of 18
66794	lipid metabolism	64.52	20 of 31
66794	lysine metabolism	64.29	27 of 42
66794	tyrosine metabolism	64.29	9 of 14
66794	degradation of sugar alcohols	62.5	10 of 16
66794	arginine metabolism	62.5	15 of 24
66794	phosphatidylethanolamine bioynthesis	61.54	8 of 13
66794	sulfate reduction	61.54	8 of 13
66794	polyamine pathway	60.87	14 of 23
66794	gallate degradation	60	3 of 5
66794	creatinine degradation	60	3 of 5
66794	vitamin K metabolism	60	3 of 5
66794	degradation of sugar acids	60	15 of 25
66794	arachidonate biosynthesis	60	3 of 5
66794	3-chlorocatechol degradation	60	3 of 5
66794	degradation of aromatic, nitrogen containing compounds	58.33	7 of 12
66794	benzoyl-CoA degradation	57.14	4 of 7
66794	nitrate assimilation	55.56	5 of 9
66794	isoprenoid biosynthesis	53.85	14 of 26
66794	3-phenylpropionate degradation	53.33	8 of 15
66794	glycogen biosynthesis	50	2 of 4
66794	aminopropanol phosphate biosynthesis	50	1 of 2
66794	glycine metabolism	50	5 of 10
66794	dolichol and dolichyl phosphate biosynthesis	50	1 of 2
66794	selenocysteine biosynthesis	50	3 of 6
66794	pantothenate biosynthesis	50	3 of 6
66794	androgen and estrogen metabolism	50	8 of 16
66794	CMP-KDO biosynthesis	50	2 of 4
66794	kanosamine biosynthesis II	50	1 of 2
66794	degradation of pentoses	46.43	13 of 28
66794	phenylpropanoid biosynthesis	46.15	6 of 13
66794	vitamin B6 metabolism	45.45	5 of 11
66794	cholesterol biosynthesis	45.45	5 of 11
66794	4-hydroxymandelate degradation	44.44	4 of 9
66794	degradation of hexoses	44.44	8 of 18
66794	bile acid biosynthesis, neutral pathway	41.18	7 of 17
66794	bacilysin biosynthesis	40	2 of 5
66794	glycogen metabolism	40	2 of 5
66794	starch degradation	40	4 of 10
66794	carnitine metabolism	37.5	3 of 8
66794	dTDPLrhamnose biosynthesis	37.5	3 of 8
66794	carotenoid biosynthesis	36.36	8 of 22
66794	arachidonic acid metabolism	33.33	6 of 18
66794	sphingosine metabolism	33.33	2 of 6
66794	(5R)-carbapenem carboxylate biosynthesis	33.33	1 of 3
66794	IAA biosynthesis	33.33	1 of 3
66794	allantoin degradation	33.33	3 of 9
66794	lipid A biosynthesis	33.33	3 of 9
66794	ascorbate metabolism	31.82	7 of 22
66794	metabolism of disaccharids	27.27	3 of 11
66794	lactate fermentation	25	1 of 4
66794	cyclohexanol degradation	25	1 of 4
66794	toluene degradation	25	1 of 4
66794	catecholamine biosynthesis	25	1 of 4
66794	chlorophyll metabolism	22.22	4 of 18

Isolation, sampling and environmental information

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
2317	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	NCBI tax ID	Score
124043	ASM5048342v1 assembly for Aneurinibacillus migulanus NRS-1138	contig	GCA_050483425	47500	48.53

GC content

2317

GC-content (mol%)48.0

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM5048342v1 assembly for Aneurinibacillus migulanus NRS-1138
NCBI: GCA_050483425

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	91.19	no
125439	gram_stain	BacteriaNetⓘ	variable	70.95	no
125439	spore_formation	BacteriaNetⓘ	yes	81.01	no
125439	motility	BacteriaNetⓘ	yes	80.14	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Purification and characterization of an antimicrobial compound against drug-resistant MRSA and VRE produced by Streptomyces levis strain HFM-2.	Verma J, Sharma M, Manhas RK.	Sci Rep	10.1038/s41598-025-10572-3	2025
Metabolism	Antimicrobial peptide gramicidin S is accumulated in granules of producer cells for storage of bacterial phosphagens.	Berditsch M, Trapp M, Afonin S, Weber C, Misiewicz J, Turkson J, Ulrich AS.	Sci Rep	10.1038/srep44324	2017
	Active site-directed proteomic probes for adenylation domains in nonribosomal peptide synthetases.	Konno S, Ishikawa F, Suzuki T, Dohmae N, Burkart MD, Kakeya H.	Chem Commun (Camb)	10.1039/c4cc09412c	2015
Phylogeny	The ability of Aneurinibacillus migulanus (Bacillus brevis) to produce the antibiotic gramicidin S is correlated with phenotype variation.	Berditsch M, Afonin S, Ulrich AS.	Appl Environ Microbiol	10.1128/aem.00881-07	2007
Pathogenicity	Supreme activity of gramicidin S against resistant, persistent and biofilm cells of staphylococci and enterococci.	Berditsch M, Afonin S, Reuster J, Lux H, Schkolin K, Babii O, Radchenko DS, Abdullah I, William N, Middel V, Strahle U, Nelson A, Valko K, Ulrich AS.	Sci Rep	10.1038/s41598-019-54212-z	2019
Pathogenicity	Damage of the bacterial cell envelope by antimicrobial peptides gramicidin S and PGLa as revealed by transmission and scanning electron microscopy.	Hartmann M, Berditsch M, Hawecker J, Ardakani MF, Gerthsen D, Ulrich AS.	Antimicrob Agents Chemother	10.1128/aac.00124-10	2010
Pathogenicity	Synergistic effect of membrane-active peptides polymyxin B and gramicidin S on multidrug-resistant strains and biofilms of Pseudomonas aeruginosa.	Berditsch M, Jager T, Strempel N, Schwartz T, Overhage J, Ulrich AS.	Antimicrob Agents Chemother	10.1128/aac.00682-15	2015
Metabolism	Fermentation and Cost-Effective 13C/15N Labeling of the Nonribosomal Peptide Gramicidin S for Nuclear Magnetic Resonance Structure Analysis.	Berditsch M, Afonin S, Steineker A, Orel N, Jakovkin I, Weber C, Ulrich AS	Appl Environ Microbiol	10.1128/AEM.00229-15	2015
Enzymology	A Multiple-Labeling Strategy for Nonribosomal Peptide Synthetases Using Active-Site-Directed Proteomic Probes for Adenylation Domains.	Ishikawa F, Suzuki T, Dohmae N, Kakeya H	Chembiochem	10.1002/cbic.201500481	2015

References

#2317	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 5759
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Aneurinibacillus migulanus (DSM 5759, NRS 1138, B1)

Name and taxonomic classification

Morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Pathway annotation

Isolation, sampling and environmental information

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_050483425

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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