Name: Aneurinibacillus migulanus DSM 2895
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 11381

Type strain:

Species: Aneurinibacillus migulanus

Culture col. no.: DSM 2895, ATCC 9999, CIP 103841, IFO 15520, IFO 3864, JCM 8504, NBRC 15520, NBRC 3864, NCIB 7096, NCTC 7096, VKM B-1722, BCRC 11717, CGMCC 1.3475, KCTC 3835, LMG 12390, LMG 15427, NCIMB 7096, NRRL NRS-1137

Strain history: O. Shida <-- ATCC 9999 <-- NCTC 7096 <-- R. L. M. Synge <-- Inst. Trop. Med., Moscow.

NCBI tax ID(s): 47500 (species)

Other strains from Aneurinibacillus migulanus

A. migulanus B2, DSM 5668, NRS 1139
A. migulanus B1, DSM 5759, NRS 1138
A. migulanus DSM 8373, NRRL NRS-606

Section

Aneurinibacillus migulanus DSM 2895 is a mesophilic bacterium that produces antibiotic compounds.

antibiotic compound production
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Paenibacillaceae*
	Genus	*Aneurinibacillus*
	Species	**Aneurinibacillus migulanus**
	Full Scientific Name (LPSN)	Aneurinibacillus migulanus (Takagi et al. 1993) Shida et al. 1996

Synonym

Bacillus migulanus

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #69480]	Motility	yes Confidence in %95.076

Information on culture and growth conditions Culture and growth conditions

[Ref.: #1161]

Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

[Ref.: #1161]

Culture medium growth

[Ref.: #1161]

Culture medium link

Medium recipe at MediaDive

[Ref.: #1161]

Culture medium composition

expand / minimize

[Ref.: #38152]

Culture medium

MEDIUM 3 - Columbia agar

[Ref.: #38152]

Culture medium growth

[Ref.: #38152]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #1161]	growth	30 °C
[Ref.: #38152]	growth	30 °C
[Ref.: #67770]	growth	30 °C

[Ref.: #1161]	Temperature range	mesophilic
[Ref.: #38152]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #69481]	Ability of spore formation	yes Confidence in %100
[Ref.: #69480]	Ability of spore formation	yes Confidence in %100

[Ref.: #1161]	Name of produced compound	gramicidin
[Ref.: #1161]	Name of produced compound	protease
[Ref.: #1161]	Name of produced compound	restriction endonuclease BbvI

[Ref.: #67770]

Observation

quinones: MK-7

	Pathway	Enzyme coverage	Annotated reactions
	Pathway annotation	Computationally determined by
[Ref.: #66794]	adipate degradation	100	2 of 2
[Ref.: #66794]	C4 and CAM-carbon fixation	100	8 of 8
[Ref.: #66794]	glycine betaine biosynthesis	100	5 of 5
[Ref.: #66794]	formaldehyde oxidation	100	3 of 3
[Ref.: #66794]	ppGpp biosynthesis	100	4 of 4
[Ref.: #66794]	lipoate biosynthesis	100	5 of 5
[Ref.: #66794]	ribulose monophosphate pathway	100	2 of 2
[Ref.: #66794]	molybdenum cofactor biosynthesis	100	9 of 9
[Ref.: #66794]	palmitate biosynthesis	100	22 of 22
[Ref.: #66794]	phenylacetate degradation (aerobic)	100	5 of 5
[Ref.: #66794]	acetate fermentation	100	4 of 4
[Ref.: #66794]	aspartate and asparagine metabolism	100	9 of 9
[Ref.: #66794]	pentose phosphate pathway	100	11 of 11
[Ref.: #66794]	ethanol fermentation	100	2 of 2
[Ref.: #66794]	gluconeogenesis	100	8 of 8
[Ref.: #66794]	coenzyme A metabolism	100	4 of 4
[Ref.: #66794]	biotin biosynthesis	100	4 of 4
[Ref.: #66794]	anapleurotic synthesis of oxalacetate	100	1 of 1
[Ref.: #66794]	folate polyglutamylation	100	1 of 1
[Ref.: #66794]	aerobactin biosynthesis	100	1 of 1
[Ref.: #66794]	methylglyoxal degradation	100	5 of 5
[Ref.: #66794]	L-lactaldehyde degradation	100	3 of 3
[Ref.: #66794]	ceramide biosynthesis	100	1 of 1
[Ref.: #66794]	suberin monomers biosynthesis	100	2 of 2
[Ref.: #66794]	UDP-GlcNAc biosynthesis	100	3 of 3
[Ref.: #66794]	CDP-diacylglycerol biosynthesis	100	2 of 2
[Ref.: #66794]	propanol degradation	100	7 of 7
[Ref.: #66794]	sulfopterin metabolism	100	4 of 4
[Ref.: #66794]	cis-vaccenate biosynthesis	100	2 of 2
[Ref.: #66794]	tetrahydrofolate metabolism	92.86	13 of 14
[Ref.: #66794]	phenylalanine metabolism	92.31	12 of 13
[Ref.: #66794]	vitamin B1 metabolism	92.31	12 of 13
[Ref.: #66794]	proline metabolism	90.91	10 of 11
[Ref.: #66794]	4-hydroxyphenylacetate degradation	90	9 of 10
[Ref.: #66794]	threonine metabolism	90	9 of 10
[Ref.: #66794]	propionate fermentation	90	9 of 10
[Ref.: #66794]	alanine metabolism	89.66	26 of 29
[Ref.: #66794]	glutamate and glutamine metabolism	89.29	25 of 28
[Ref.: #66794]	serine metabolism	88.89	8 of 9
[Ref.: #66794]	valine metabolism	88.89	8 of 9
[Ref.: #66794]	CO2 fixation in Crenarchaeota	88.89	8 of 9
[Ref.: #66794]	chorismate metabolism	88.89	8 of 9
[Ref.: #66794]	ketogluconate metabolism	87.5	7 of 8
[Ref.: #66794]	flavin biosynthesis	86.67	13 of 15
[Ref.: #66794]	reductive acetyl coenzyme A pathway	85.71	6 of 7
[Ref.: #66794]	vitamin B12 metabolism	85.29	29 of 34
[Ref.: #66794]	phenol degradation	85	17 of 20
[Ref.: #66794]	methionine metabolism	84.62	22 of 26
[Ref.: #66794]	leucine metabolism	84.62	11 of 13
[Ref.: #66794]	NAD metabolism	83.33	15 of 18
[Ref.: #66794]	1,4-dihydroxy-6-naphthoate biosynthesis	83.33	5 of 6
[Ref.: #66794]	pyrimidine metabolism	82.22	37 of 45
[Ref.: #66794]	Entner Doudoroff pathway	80	8 of 10
[Ref.: #66794]	factor 420 biosynthesis	80	4 of 5
[Ref.: #66794]	cellulose degradation	80	4 of 5
[Ref.: #66794]	peptidoglycan biosynthesis	80	12 of 15
[Ref.: #66794]	metabolism of amino sugars and derivatives	80	4 of 5
[Ref.: #66794]	tryptophan metabolism	78.95	30 of 38
[Ref.: #66794]	heme metabolism	78.57	11 of 14
[Ref.: #66794]	photosynthesis	78.57	11 of 14
[Ref.: #66794]	citric acid cycle	78.57	11 of 14
[Ref.: #66794]	d-mannose degradation	77.78	7 of 9
[Ref.: #66794]	purine metabolism	77.66	73 of 94
[Ref.: #66794]	urea cycle	76.92	10 of 13
[Ref.: #66794]	butanoate fermentation	75	3 of 4
[Ref.: #66794]	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	75	6 of 8
[Ref.: #66794]	isoleucine metabolism	75	6 of 8
[Ref.: #66794]	cardiolipin biosynthesis	71.43	5 of 7
[Ref.: #66794]	ubiquinone biosynthesis	71.43	5 of 7
[Ref.: #66794]	glutathione metabolism	71.43	10 of 14
[Ref.: #66794]	glycolysis	70.59	12 of 17
[Ref.: #66794]	myo-inositol biosynthesis	70	7 of 10
[Ref.: #66794]	coenzyme M biosynthesis	70	7 of 10
[Ref.: #66794]	histidine metabolism	68.97	20 of 29
[Ref.: #66794]	non-pathway related	68.42	26 of 38
[Ref.: #66794]	oxidative phosphorylation	68.13	62 of 91
[Ref.: #66794]	acetyl CoA biosynthesis	66.67	2 of 3
[Ref.: #66794]	acetoin degradation	66.67	2 of 3
[Ref.: #66794]	octane oxidation	66.67	2 of 3
[Ref.: #66794]	enterobactin biosynthesis	66.67	2 of 3
[Ref.: #66794]	methane metabolism	66.67	2 of 3
[Ref.: #66794]	glycolate and glyoxylate degradation	66.67	4 of 6
[Ref.: #66794]	cyanate degradation	66.67	2 of 3
[Ref.: #66794]	cysteine metabolism	66.67	12 of 18
[Ref.: #66794]	lipid metabolism	64.52	20 of 31
[Ref.: #66794]	lysine metabolism	64.29	27 of 42
[Ref.: #66794]	tyrosine metabolism	64.29	9 of 14
[Ref.: #66794]	degradation of sugar alcohols	62.5	10 of 16
[Ref.: #66794]	arginine metabolism	62.5	15 of 24
[Ref.: #66794]	phosphatidylethanolamine bioynthesis	61.54	8 of 13
[Ref.: #66794]	sulfate reduction	61.54	8 of 13
[Ref.: #66794]	polyamine pathway	60.87	14 of 23
[Ref.: #66794]	gallate degradation	60	3 of 5
[Ref.: #66794]	creatinine degradation	60	3 of 5
[Ref.: #66794]	vitamin K metabolism	60	3 of 5
[Ref.: #66794]	degradation of sugar acids	60	15 of 25
[Ref.: #66794]	arachidonate biosynthesis	60	3 of 5
[Ref.: #66794]	3-chlorocatechol degradation	60	3 of 5
[Ref.: #66794]	degradation of aromatic, nitrogen containing compounds	58.33	7 of 12
[Ref.: #66794]	benzoyl-CoA degradation	57.14	4 of 7
[Ref.: #66794]	nitrate assimilation	55.56	5 of 9
[Ref.: #66794]	isoprenoid biosynthesis	53.85	14 of 26
[Ref.: #66794]	3-phenylpropionate degradation	53.33	8 of 15
[Ref.: #66794]	glycogen biosynthesis	50	2 of 4
[Ref.: #66794]	aminopropanol phosphate biosynthesis	50	1 of 2
[Ref.: #66794]	glycine metabolism	50	5 of 10
[Ref.: #66794]	dolichol and dolichyl phosphate biosynthesis	50	1 of 2
[Ref.: #66794]	selenocysteine biosynthesis	50	3 of 6
[Ref.: #66794]	pantothenate biosynthesis	50	3 of 6
[Ref.: #66794]	androgen and estrogen metabolism	50	8 of 16
[Ref.: #66794]	CMP-KDO biosynthesis	50	2 of 4
[Ref.: #66794]	kanosamine biosynthesis II	50	1 of 2
[Ref.: #66794]	degradation of pentoses	46.43	13 of 28
[Ref.: #66794]	phenylpropanoid biosynthesis	46.15	6 of 13
[Ref.: #66794]	vitamin B6 metabolism	45.45	5 of 11
[Ref.: #66794]	cholesterol biosynthesis	45.45	5 of 11
[Ref.: #66794]	4-hydroxymandelate degradation	44.44	4 of 9
[Ref.: #66794]	degradation of hexoses	44.44	8 of 18
[Ref.: #66794]	bile acid biosynthesis, neutral pathway	41.18	7 of 17
[Ref.: #66794]	bacilysin biosynthesis	40	2 of 5
[Ref.: #66794]	glycogen metabolism	40	2 of 5
[Ref.: #66794]	starch degradation	40	4 of 10
[Ref.: #66794]	carnitine metabolism	37.5	3 of 8
[Ref.: #66794]	dTDPLrhamnose biosynthesis	37.5	3 of 8
[Ref.: #66794]	carotenoid biosynthesis	36.36	8 of 22
[Ref.: #66794]	arachidonic acid metabolism	33.33	6 of 18
[Ref.: #66794]	sphingosine metabolism	33.33	2 of 6
[Ref.: #66794]	(5R)-carbapenem carboxylate biosynthesis	33.33	1 of 3
[Ref.: #66794]	IAA biosynthesis	33.33	1 of 3
[Ref.: #66794]	allantoin degradation	33.33	3 of 9
[Ref.: #66794]	lipid A biosynthesis	33.33	3 of 9
[Ref.: #66794]	ascorbate metabolism	31.82	7 of 22
[Ref.: #66794]	metabolism of disaccharids	27.27	3 of 11
[Ref.: #66794]	lactate fermentation	25	1 of 4
[Ref.: #66794]	cyclohexanol degradation	25	1 of 4
[Ref.: #66794]	toluene degradation	25	1 of 4
[Ref.: #66794]	catecholamine biosynthesis	25	1 of 4
[Ref.: #66794]	chlorophyll metabolism	22.22	4 of 18
		Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #67770]	Sample type/isolated from	Garden soil

[Ref.: #38152]	Geographic location (country and/or sea, region)	Moscow
[Ref.: #38152]	Country	Russia
[Ref.: #38152]	Country ISO 3 Code	RUS
[Ref.: #38152]	Continent	Europe
* marker position based on {}

[Ref.: #69479]

Global distribution of 16S sequence X94195 (>99% sequence identity) for Aneurinibacillus from Microbeatlas

Aquatic Samples	218
Soil Samples	881
Animal Samples	736
Plant Samples	112
Total Samples	1947

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #1161]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Aneurinibacillus migulanus gene for 16S rRNA, partial sequence, strain: JCM 8504	LC379068	1461	ENA	47500 tax ID
[Ref.: #20218]	Aneurinibacillus migulanus DNA for 16S ribosomal RNA	D78462	1422	ENA	47500 tax ID	*
[Ref.: #20218]	Aneurinibacillus migulanus strain B0270 16S ribosomal RNA gene, partial sequence	KC527683	1006	ENA	47500 tax ID	*
[Ref.: #20218]	Aneurinibacillus migulanus gene for 16S rRNA, partial sequence, strain:DSM 2895T	AB112723	1489	ENA	47500 tax ID	*
[Ref.: #20218]	Aneurinibacillus migulanus isolate Am25 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence	EU723617	1157	ENA	47500 tax ID	*
[Ref.: #20218]	B.migulanus 16S ribosomal RNA	X94195	1483	ENA	47500 tax ID	*
[Ref.: #20218]	Aneurinibacillus migulanus gene for 16S rRNA, partial sequence, strain: NBRC 15520	AB680889	1464	ENA	47500 tax ID	*

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Aneurinibacillus migulanus NBRC 15520	GCA_006539965	contig	GenBank	47500 tax ID
[Ref.: #67770]	Aneurinibacillus migulanus DSM 2895	GCA_900100065	scaffold	GenBank	47500 tax ID
[Ref.: #67770]	Aneurinibacillus migulanus NCTC 7096	GCA_000878905	scaffold	GenBank	47500 tax ID
[Ref.: #67770]	Aneurinibacillus migulanus DSM 2895	GCA_001274715	scaffold	GenBank	47500 tax ID
[Ref.: #66792]	Aneurinibacillus migulanus strain NBRC 15520	47500.16	wgs	PATRIC	47500 tax ID	*
[Ref.: #66792]	Aneurinibacillus migulanus DSM 2895	2615840722	draft	JGI IMG/M	47500 tax ID	*

[Ref.: #1161]	GC-content	42.5 mol%
[Ref.: #67770]	GC-content	42.5 mol%	high performance liquid chromatography (HPLC)
[Ref.: #67770]	GC-content	51.1 mol%	Buoyant density centrifugation (BD)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Aneurinibacillus migulanus DSM 2895 NCBI: GCA_001274715.1
[Ref.: #69480]	gram-positive	yes	100	yes
[Ref.: #69480]	spore-forming	yes	100	yes
[Ref.: #69480]	anaerobic	no	98.968	no
[Ref.: #69480]	thermophile	no	86.021	yes
[Ref.: #69480]	motile	yes	95.076	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Aneurinibacillus migulanus DSM 2895 NCBI: GCA_001274715.1
[Ref.: #69481]	spore-forming	yes	100	no

Availability in culture collections External links

[Ref.: #1161]

Culture collection no.

DSM 2895, ATCC 9999, CIP 103841, IFO 15520, IFO 3864, JCM 8504, NBRC 15520, NBRC 3864, NCIB 7096, NCTC 7096, VKM B-1722, BCRC 11717, CGMCC 1.3475, KCTC 3835, LMG 12390, LMG 15427, NCIMB 7096, NRRL NRS-1137

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Biotechnology	Increased Biological Activity of Aneurinibacillus migulanus Strains Correlates with the Production of New Gramicidin Secondary Metabolites.	Alenezi FN, Rekik I, Chenari Bouket A, Luptakova L, Weitz HJ, Rateb ME, Jaspars M, Woodward S, Belbahri L	Front Microbiol	10.3389/fmicb.2017.00517	2017	*
Metabolism	Strain-level diversity of secondary metabolism in the biocontrol species Aneurinibacillus migulanus.	Alenezi FN, Rekik I, Belka M, Ibrahim AF, Luptakova L, Jaspars M, Woodward S, Belbahri L	Microbiol Res	10.1016/j.micres.2015.10.007	2015	*
Phylogeny	Aneurinibacillus humi sp. nov., Isolated from Soil Collected in Ukraine.	Lee K, Lee SS	Curr Microbiol	10.1007/s00284-015-0930-7	2015	*
Genetics	High-Quality Draft Genome Sequence of Aneurinibacillus migulanus ATCC 9999T (DSM 2895), a Gramicidin S-Producing Bacterium Isolated from Garden Soil.	Wang JP, Liu B, Liu GH, Ge CB, Xiao RF, Zheng XF, Shi H	Genome Announc	10.1128/genomeA.01227-15	2015	*
Enzymology	A Multiple-Labeling Strategy for Nonribosomal Peptide Synthetases Using Active-Site-Directed Proteomic Probes for Adenylation Domains.	Ishikawa F, Suzuki T, Dohmae N, Kakeya H	Chembiochem	10.1002/cbic.201500481	2015	*
Genetics	Draft Genome Sequence of Aneurinibacillus migulanus NCTC 7096.	Alenezi FN, Weitz HJ, Belbahri L, Nidhal J, Luptakova L, Jaspars M, Woodward S	Genome Announc	10.1128/genomeA.00234-15	2015	*
Phenotype	The ability of Aneurinibacillus migulanus (Bacillus brevis) to produce the antibiotic gramicidin S is correlated with phenotype variation.	Berditsch M, Afonin S, Ulrich AS	Appl Environ Microbiol	10.1128/AEM.00881-07	2007	*

References

#1161

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 2895

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#38152 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#66794

Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Aneurinibacillus migulanus in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF