Aneurinibacillus migulanus (DSM 2895, ATCC 9999, CIP 103841)

Name: Aneurinibacillus migulanus (DSM 2895, ATCC 9999, CIP 103841)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 11381

Type strain

Culture col. no. DSM 2895 ATCC 9999 CIP 103841 IFO 15520 IFO 3864 13 more

NCBI tax ID(s) 47500

Special Collections DSMZ JCM KCTC CIP

History <- Schering AG <- ATCC <- NCTC <- R.L.M. Syndge <- Inst. Trop. Med., Moscow (Bacillus brevis) O. Shida <-- ATCC 9999 <-- NCTC 7096 <-- R. L. M. Synge <-- Inst. Trop. Med., Moscow. CIP <- 1993, O. Shida, Res. Lab. Higeta Shoyu Co, Japan <- ATCC, Bacillus brevis <- NCTC <- R. Synge

Links

Aneurinibacillus migulanus DSM 2895 is a bacterium that produces antibiotic compounds.

antibiotic compound production genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 7123

LMG 12390,ATCC 9999,CIP 103841,DSM 2895,IFO 15520,IFO 3864,JCM 8504,LMG 15427,NCIMB 7096,NCTC 7096,VKM B-1722,NCIB 7096,CCRC 11717,NRRL NRS-1137,CCM 4525,NBRC 15520,BCRC 11717,NBRC 3864,KCTC 3835,NCIM 2531,VTT E-97187

@ref 20215

Last LPSN update 2026-02-09 11:20:32

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Caryophanales

Family Paenibacillaceae

Genus Aneurinibacillus

Species Aneurinibacillus migulanus

Full scientific name Aneurinibacillus migulanus (Takagi et al. 1993) Shida et al. 1996

Synonyms (1)

Bacillus migulanus

BacDive ID	Other strains from **Aneurinibacillus migulanus** (3)	Type strain
11382	A. migulanus B2, DSM 5668, NRS 1139
11383	A. migulanus B1, DSM 5759, NRS 1138
11384	A. migulanus DSM 8373, NRRL NRS-606

Morphology

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
1161	NUTRIENT AGAR (DSMZ Medium 1)	Medium recipe at MediaDive	Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
38152	MEDIUM 3 - Columbia agar		Columbia agar (39.000 g);distilled water (1000.000 ml)
120951	CIP Medium 3	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
1161	positive	growth	30
38152	positive	growth	30
67770	positive	growth	30

Physiology and metabolism

Spore formation

@ref	Spore formation	Confidence
125439		91.5

Compound production

@ref	Compound
1161	gramicidin
1161	protease
1161	restriction endonuclease BbvI

Observation

67770

Observationquinones: MK-7

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	adipate degradation	100	2 of 2
66794	C4 and CAM-carbon fixation	100	8 of 8
66794	glycine betaine biosynthesis	100	5 of 5
66794	formaldehyde oxidation	100	3 of 3
66794	ppGpp biosynthesis	100	4 of 4
66794	lipoate biosynthesis	100	5 of 5
66794	ribulose monophosphate pathway	100	2 of 2
66794	molybdenum cofactor biosynthesis	100	9 of 9
66794	palmitate biosynthesis	100	22 of 22
66794	phenylacetate degradation (aerobic)	100	5 of 5
66794	acetate fermentation	100	4 of 4
66794	aspartate and asparagine metabolism	100	9 of 9
66794	pentose phosphate pathway	100	11 of 11
66794	ethanol fermentation	100	2 of 2
66794	gluconeogenesis	100	8 of 8
66794	coenzyme A metabolism	100	4 of 4
66794	biotin biosynthesis	100	4 of 4
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	folate polyglutamylation	100	1 of 1
66794	aerobactin biosynthesis	100	1 of 1
66794	methylglyoxal degradation	100	5 of 5
66794	L-lactaldehyde degradation	100	3 of 3
66794	ceramide biosynthesis	100	1 of 1
66794	suberin monomers biosynthesis	100	2 of 2
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	propanol degradation	100	7 of 7
66794	sulfopterin metabolism	100	4 of 4
66794	cis-vaccenate biosynthesis	100	2 of 2
66794	tetrahydrofolate metabolism	92.86	13 of 14
66794	phenylalanine metabolism	92.31	12 of 13
66794	vitamin B1 metabolism	92.31	12 of 13
66794	proline metabolism	90.91	10 of 11
66794	4-hydroxyphenylacetate degradation	90	9 of 10
66794	threonine metabolism	90	9 of 10
66794	propionate fermentation	90	9 of 10
66794	alanine metabolism	89.66	26 of 29
66794	glutamate and glutamine metabolism	89.29	25 of 28
66794	serine metabolism	88.89	8 of 9
66794	valine metabolism	88.89	8 of 9
66794	CO2 fixation in Crenarchaeota	88.89	8 of 9
66794	chorismate metabolism	88.89	8 of 9
66794	ketogluconate metabolism	87.5	7 of 8
66794	flavin biosynthesis	86.67	13 of 15
66794	reductive acetyl coenzyme A pathway	85.71	6 of 7
66794	vitamin B12 metabolism	85.29	29 of 34
66794	phenol degradation	85	17 of 20
66794	methionine metabolism	84.62	22 of 26
66794	leucine metabolism	84.62	11 of 13
66794	NAD metabolism	83.33	15 of 18
66794	1,4-dihydroxy-6-naphthoate biosynthesis	83.33	5 of 6
66794	pyrimidine metabolism	82.22	37 of 45
66794	Entner Doudoroff pathway	80	8 of 10
66794	factor 420 biosynthesis	80	4 of 5
66794	cellulose degradation	80	4 of 5
66794	peptidoglycan biosynthesis	80	12 of 15
66794	metabolism of amino sugars and derivatives	80	4 of 5
66794	tryptophan metabolism	78.95	30 of 38
66794	heme metabolism	78.57	11 of 14
66794	photosynthesis	78.57	11 of 14
66794	citric acid cycle	78.57	11 of 14
66794	d-mannose degradation	77.78	7 of 9
66794	purine metabolism	77.66	73 of 94
66794	urea cycle	76.92	10 of 13
66794	butanoate fermentation	75	3 of 4
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	75	6 of 8
66794	isoleucine metabolism	75	6 of 8
66794	cardiolipin biosynthesis	71.43	5 of 7
66794	ubiquinone biosynthesis	71.43	5 of 7
66794	glutathione metabolism	71.43	10 of 14
66794	glycolysis	70.59	12 of 17
66794	myo-inositol biosynthesis	70	7 of 10
66794	coenzyme M biosynthesis	70	7 of 10
66794	histidine metabolism	68.97	20 of 29
66794	non-pathway related	68.42	26 of 38
66794	oxidative phosphorylation	68.13	62 of 91
66794	acetyl CoA biosynthesis	66.67	2 of 3
66794	acetoin degradation	66.67	2 of 3
66794	octane oxidation	66.67	2 of 3
66794	enterobactin biosynthesis	66.67	2 of 3
66794	methane metabolism	66.67	2 of 3
66794	glycolate and glyoxylate degradation	66.67	4 of 6
66794	cyanate degradation	66.67	2 of 3
66794	cysteine metabolism	66.67	12 of 18
66794	lipid metabolism	64.52	20 of 31
66794	lysine metabolism	64.29	27 of 42
66794	tyrosine metabolism	64.29	9 of 14
66794	degradation of sugar alcohols	62.5	10 of 16
66794	arginine metabolism	62.5	15 of 24
66794	phosphatidylethanolamine bioynthesis	61.54	8 of 13
66794	sulfate reduction	61.54	8 of 13
66794	polyamine pathway	60.87	14 of 23
66794	gallate degradation	60	3 of 5
66794	creatinine degradation	60	3 of 5
66794	vitamin K metabolism	60	3 of 5
66794	degradation of sugar acids	60	15 of 25
66794	arachidonate biosynthesis	60	3 of 5
66794	3-chlorocatechol degradation	60	3 of 5
66794	degradation of aromatic, nitrogen containing compounds	58.33	7 of 12
66794	benzoyl-CoA degradation	57.14	4 of 7
66794	nitrate assimilation	55.56	5 of 9
66794	isoprenoid biosynthesis	53.85	14 of 26
66794	3-phenylpropionate degradation	53.33	8 of 15
66794	glycogen biosynthesis	50	2 of 4
66794	aminopropanol phosphate biosynthesis	50	1 of 2
66794	glycine metabolism	50	5 of 10
66794	dolichol and dolichyl phosphate biosynthesis	50	1 of 2
66794	selenocysteine biosynthesis	50	3 of 6
66794	pantothenate biosynthesis	50	3 of 6
66794	androgen and estrogen metabolism	50	8 of 16
66794	CMP-KDO biosynthesis	50	2 of 4
66794	kanosamine biosynthesis II	50	1 of 2
66794	degradation of pentoses	46.43	13 of 28
66794	phenylpropanoid biosynthesis	46.15	6 of 13
66794	vitamin B6 metabolism	45.45	5 of 11
66794	cholesterol biosynthesis	45.45	5 of 11
66794	4-hydroxymandelate degradation	44.44	4 of 9
66794	degradation of hexoses	44.44	8 of 18
66794	bile acid biosynthesis, neutral pathway	41.18	7 of 17
66794	bacilysin biosynthesis	40	2 of 5
66794	glycogen metabolism	40	2 of 5
66794	starch degradation	40	4 of 10
66794	carnitine metabolism	37.5	3 of 8
66794	dTDPLrhamnose biosynthesis	37.5	3 of 8
66794	carotenoid biosynthesis	36.36	8 of 22
66794	arachidonic acid metabolism	33.33	6 of 18
66794	sphingosine metabolism	33.33	2 of 6
66794	(5R)-carbapenem carboxylate biosynthesis	33.33	1 of 3
66794	IAA biosynthesis	33.33	1 of 3
66794	allantoin degradation	33.33	3 of 9
66794	lipid A biosynthesis	33.33	3 of 9
66794	ascorbate metabolism	31.82	7 of 22
66794	metabolism of disaccharids	27.27	3 of 11
66794	lactate fermentation	25	1 of 4
66794	cyclohexanol degradation	25	1 of 4
66794	toluene degradation	25	1 of 4
66794	catecholamine biosynthesis	25	1 of 4
66794	chlorophyll metabolism	22.22	4 of 18

Isolation, sampling and environmental information

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
38152		Moscow	Russia	RUS	Europe
67770	Garden soil
120951	Environment, Soil	Moscow	Russian Federation	RUS	Europe

Taxonmaps

69479

Global distribution of 16S sequence X94195 (>99% sequence identity) for Aneurinibacillus from Microbeatlas

Aquatic Samples	218
Soil Samples	881
Animal Samples	736
Plant Samples	112
Total Samples	1947

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
1161	1	Risk group (German classification) According to TRBA 466
120951	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	Aneurinibacillus migulanus ATCC 9999	complete			2563366674	47500	77.61
67770	ASM127471v1 assembly for Aneurinibacillus migulanus DSM 2895	scaffold	GCA_001274715	47500.9	2645727852	47500	66.09
67770	IMG-taxon 2615840722 annotated assembly for Aneurinibacillus migulanus DSM 2895	scaffold	GCA_900100065	47500.13	2615840722	47500	58.22
67770	Amig1.0 assembly for Aneurinibacillus migulanus NCTC 7096	scaffold	GCA_000878905	47500.8	2627854104	47500	34.62
67770	ASM653996v1 assembly for Aneurinibacillus migulanus NBRC 15520	contig	GCA_006539965	47500.16		47500

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Aneurinibacillus migulanus DNA for 16S ribosomal RNA	D78462	1422	47500
20218	Aneurinibacillus migulanus strain B0270 16S ribosomal RNA gene, partial sequence	KC527683	1006	47500
20218	Aneurinibacillus migulanus gene for 16S rRNA, partial sequence, strain:DSM 2895T	AB112723	1489	47500
20218	Aneurinibacillus migulanus isolate Am25 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence	EU723617	1157	47500
20218	B.migulanus 16S ribosomal RNA	X94195	1483	47500
20218	Aneurinibacillus migulanus gene for 16S rRNA, partial sequence, strain: NBRC 15520	AB680889	1464	47500
67770	Aneurinibacillus migulanus gene for 16S rRNA, partial sequence, strain: JCM 8504	LC379068	1461	47500
124043	Alteribacter salitolerans strain APA H-16(1) 16S ribosomal RNA gene, partial sequence.	MT509985	703	47500
124043	Aneurinibacillus migulanus strain DSM 2895 16S ribosomal RNA gene, partial sequence.	OQ870296	1427	47500

GC content

@ref	GC-content (mol%)	Method
1161	42.5
67770	42.5	high performance liquid chromatography (HPLC)
67770	51.1	Buoyant density centrifugation (BD)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Aneurinibacillus migulanus DSM 2895
NCBI: GCA_900100065

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	91.50	no
125439	motility	BacteriaNetⓘ	yes	84.80	no
125439	gram_stain	BacteriaNetⓘ	variable	85.10	no
125439	oxygen_tolerance	BacteriaNetⓘ	facultative anaerobe	80.70	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Aneurinibacillus migulanus strain DSM 2895
PATRIC: 47500.9

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	57.74	no
125438	anaerobic	anaerobicⓘ	no	95.90	no
125438	spore-forming	spore-formingⓘ	yes	86.82	no
125438	aerobic	aerobicⓘ	yes	75.66	no
125438	thermophilic	thermophileⓘ	no	87.73	no
125438	flagellated	motile2+ⓘ	yes	79.90	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Genetics	Genome-Driven Discovery of Enzymes with Industrial Implications from the Genus Aneurinibacillus.	Kamli MR, Alzahrani NAY, Hajrah NH, Sabir JSM, Malik A.	Microorganisms	10.3390/microorganisms9030499	2021
Metabolism	Metatranscriptome Profiling of a Specialized Microbial Consortium during the Degradation of Nixtamalized Maize Pericarp.	Serrano-Gamboa JG, Focil-Espinosa CA, Cabello-Yeves PJ, Coutinho FH, Rojas-Herrera RA, Sanchez-Gonzalez MN.	Microbiol Spectr	10.1128/spectrum.02318-21	2022
	Isolation and characterization of arsenic-resistant bacteria and possible application in bioremediation.	Dey U, Chatterjee S, Mondal NK.	Biotechnol Rep (Amst)	10.1016/j.btre.2016.02.002	2016
Metabolism	Fermentation and Cost-Effective 13C/15N Labeling of the Nonribosomal Peptide Gramicidin S for Nuclear Magnetic Resonance Structure Analysis.	Berditsch M, Afonin S, Steineker A, Orel N, Jakovkin I, Weber C, Ulrich AS.	Appl Environ Microbiol	10.1128/aem.00229-15	2015
	Development of a chemical scaffold for inhibiting nonribosomal peptide synthetases in live bacterial cells.	Ishikawa F, Konno S, Kakeya H, Tanabe G.	Beilstein J Org Chem	10.3762/bjoc.20.39	2024
	Chemoproteomic Profiling of Adenylation Domain Functions in Gramicidin S-Producing Non-ribosomal Peptide Synthetases.	Ishikawa F, Tanabe G.	Methods Mol Biol	10.1007/978-1-0716-3214-7_4	2023
	Exploring the Substrate Flexibility of GrsB Thioesterase Leads to the Structural Reassignment of a Gramicidin S Variant.	Konno S, Mizuguchi T, Suzuki A, Tanaka M, Ishikawa F, Taguchi A, Taniguchi A, Tanabe G, Hayashi Y.	Chembiochem	10.1002/cbic.202500412	2025
	Exploring a chemical scaffold for rapid and selective photoaffinity labelling of non-ribosomal peptide synthetases in living bacterial cells.	Ishikawa F, Konno S, Uchiyama Y, Kakeya H, Tanabe G.	Philos Trans R Soc Lond B Biol Sci	10.1098/rstb.2022.0026	2023
	Targeting Siderophore Biosynthesis to Thwart Microbial Growth.	Rocha BM, Pinto E, Sousa E, Resende DISP.	Int J Mol Sci	10.3390/ijms26083611	2025
Enzymology	Affinity Purification Method for the Identification of Nonribosomal Peptide Biosynthetic Enzymes Using a Synthetic Probe for Adenylation Domains.	Ishikawa F, Kakeya H.	Methods Mol Biol	10.1007/978-1-4939-3375-4_4	2016
	Active site-directed proteomic probes for adenylation domains in nonribosomal peptide synthetases.	Konno S, Ishikawa F, Suzuki T, Dohmae N, Burkart MD, Kakeya H.	Chem Commun (Camb)	10.1039/c4cc09412c	2015
	Identification of novel Gramicidin S analogs from Aneurinibacillus aneurinilyticus isolated from corn steep liquor with potential antifungal activity.	Lvova K, Marcos M, Perez-Cid B, Vecino X, Cruz JM, Moldes AB.	Microb Cell Fact	10.1186/s12934-025-02835-5	2025
	Biological control of Dothistroma needle blight on pine with Aneurinibacillus migulanus	Alenezi FN, Fraser S, Belka M, Dogmus TH, Heckova Z, Oskay F, Belbahri L, Woodward S.	For Pathol	10.1111/efp.12237	2016
Biotechnology	Increased Biological Activity of Aneurinibacillus migulanus Strains Correlates with the Production of New Gramicidin Secondary Metabolites.	Alenezi FN, Rekik I, Chenari Bouket A, Luptakova L, Weitz HJ, Rateb ME, Jaspars M, Woodward S, Belbahri L	Front Microbiol	10.3389/fmicb.2017.00517	2017
Metabolism	Strain-level diversity of secondary metabolism in the biocontrol species Aneurinibacillus migulanus.	Alenezi FN, Rekik I, Belka M, Ibrahim AF, Luptakova L, Jaspars M, Woodward S, Belbahri L	Microbiol Res	10.1016/j.micres.2015.10.007	2015
Genetics	High-Quality Draft Genome Sequence of Aneurinibacillus migulanus ATCC 9999T (DSM 2895), a Gramicidin S-Producing Bacterium Isolated from Garden Soil.	Wang JP, Liu B, Liu GH, Ge CB, Xiao RF, Zheng XF, Shi H	Genome Announc	10.1128/genomeA.01227-15	2015
Enzymology	A Multiple-Labeling Strategy for Nonribosomal Peptide Synthetases Using Active-Site-Directed Proteomic Probes for Adenylation Domains.	Ishikawa F, Suzuki T, Dohmae N, Kakeya H	Chembiochem	10.1002/cbic.201500481	2015
Genetics	Draft Genome Sequence of Aneurinibacillus migulanus NCTC 7096.	Alenezi FN, Weitz HJ, Belbahri L, Nidhal J, Luptakova L, Jaspars M, Woodward S	Genome Announc	10.1128/genomeA.00234-15	2015
Phenotype	The ability of Aneurinibacillus migulanus (Bacillus brevis) to produce the antibiotic gramicidin S is correlated with phenotype variation.	Berditsch M, Afonin S, Ulrich AS	Appl Environ Microbiol	10.1128/AEM.00881-07	2007
Phylogeny	Aneurinibacillus humi sp. nov., Isolated from Soil Collected in Ukraine.	Lee K, Lee SS	Curr Microbiol	10.1007/s00284-015-0930-7	2015

References

#1161	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 2895
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#38152	; Curators of the CIP;
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#120951	Collection of Institut Pasteur ; Curators of the CIP; CIP 103841
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Aneurinibacillus migulanus (DSM 2895, ATCC 9999, CIP 103841)

Name and taxonomic classification

Morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Spore formation

Compound production

Observation

Pathway annotation

Isolation, sampling and environmental information

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_001274715

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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