Herbaspirillum huttiense subsp. putei (DSM 10281, ATCC 14670, JCM 21423)

Name: Herbaspirillum huttiense subsp. putei (DSM 10281, ATCC 14670, JCM 21423)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 11358

Type strain

Strain Designation 266

Culture col. no. DSM 10281 ATCC 14670 JCM 21423 CCUG 20333 LMG 2199 5 more

NCBI tax ID(s) 863372 86172

Special Collections DSMZ CCUG JCM CIP

Links

Herbaspirillum huttiense subsp. putei 266 is an obligate aerobe, mesophilic, Gram-negative prokaryote that was isolated from distilled water.

Gram-negative motile rod-shaped obligate aerobe mesophilic 16S sequence

Name and taxonomic classification

SI-ID 4110

LMG 2199,ATCC 14670,NCIB 9462,CCUG 20333,CIP 103296,DSM 10281,NCIMB 9462,IAM 14941,NCCB 95044,LMD 95.44,BCRC 15828,CCRC 15828,NBRC 102521,JCM 21423

@ref 20215

Last LPSN update 2025-12-16 09:49:34

Domain Pseudomonadati

Phylum Pseudomonadota

Class Betaproteobacteria

Order Burkholderiales

Family Oxalobacteraceae

Genus Herbaspirillum

Species Herbaspirillum huttiense subsp. putei

Full scientific name Herbaspirillum huttiense subsp. putei (Ding and Yokota 2004) Dobritsa et al. 2010

Synonyms (1)

Herbaspirillum putei

BacDive ID	Other strains from **Herbaspirillum huttiense subsp. putei** (1)	Type strain
11368	H. huttiense subsp. putei 7-2, DSM 21883, ATCC BAA-806, CCUG 49453, ... (type strain)

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility
119992	negative	rod-shaped

Pigmentation

@ref	Production	Name
119992		Pyocyanin

Multimedia

@ref:	3877
multimedia content:	DSM_10281.jpg
multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_10281.jpg
intellectual property rights:	© Leibniz-Institut DSMZ
manual_annotation:	1

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
3877	CASO AGAR (MERCK 105458) (DSMZ Medium 220)	Medium recipe at MediaDive	Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water
34240	MEDIUM 18 - for Hydrogenophaga, Vogesella and Pseudomonas genera		Distilled water make up to (1000.000 ml);Sodium chloride (3.000 g);Agar(12.000 g);Yeast extract (3.000 g);Peptone (0.600 g)
119992	CIP Medium 72	Medium recipe at CIP
119992	CIP Medium 18	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
3877	positive	growth	28	mesophilic
34240	positive	growth	30	mesophilic
67770	positive	growth	25	mesophilic
119992	positive	growth	30	mesophilic
119992	negative	growth	5
119992	negative	growth	41
119992	negative	growth	45

Physiology and metabolism

Oxygen tolerance

119992

Oxygen toleranceobligate aerobe

Observation

67770

Observationquinones: Q-8

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
119992	16947 ChEBI	citrate	+	carbon source
119992	4853 ChEBI	esculin	-	hydrolysis
119992	17632 ChEBI	nitrate	-	reduction
119992	17632 ChEBI	nitrate	-	respiration
119992	16301 ChEBI	nitrite	-	reduction
119992	15882 ChEBI	phenol	+	degradation

Antibiotic resistance

@ref	Metabolite	Is antibiotic	Is sensitive	Is resistant
119992	0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)

Enzymes

@ref	Value	Activity	Ec
119992	alcohol dehydrogenase	-	1.1.1.1
119992	amylase	-
119992	beta-galactosidase	+	3.2.1.23
119992	caseinase	-	3.4.21.50
119992	catalase	-	1.11.1.6
119992	gelatinase	-
119992	lecithinase	-
119992	lysine decarboxylase	-	4.1.1.18
119992	ornithine decarboxylase	-	4.1.1.17
119992	oxidase	+
119992	tween esterase	-

API biotype100

@ref	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
119992	not determinedn.d.	+	+	+	-	+	-	-	-	-	-	-	+	+	+	-	-	-	-	-	+	+	+	-	-	+	-	+	+	+	-	-	+	-	+	-	-	+	+	-	+	+	-	-	+	+	+	-	+	+	+	+	+	-	+	+	+	+	+	-	+	+	-	+	+	+	-	-	+	-	-	-	-	-	+	-	+	+	+	-	+	+	-	+	-	+	-	+	-	+	+	+	+	-	+	+	+	+	+	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2
#Environmental	#Aquatic
#Engineered	#Industrial
#Engineered	#Treatment

Isolation

@ref	Sample type	Country	Country ISO 3 Code	Continent
3877	distilled water	New Zealand	NZL	Australia and Oceania
47134	Water,distilled	New Zealand	NZL	Australia and Oceania
67770	Distilled water	New Zealand	NZL	Australia and Oceania
119992	Environment, Distilled water

Taxonmaps

69479

Global distribution of 16S sequence AB021366 (>99% sequence identity) for Herbaspirillum from Microbeatlas

Aquatic Samples	342
Soil Samples	197
Animal Samples	743
Plant Samples	411
Total Samples	1693

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
3877	1	Risk group (German classification) According to TRBA 466
119992	1	Risk group (French classification) According to TRBA 466

Sequence information

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
3877	Herbaspirillum huttiense gene for 16S rRNA, strain: ATCC 14670	AB021366	1490	863372
124043	Herbaspirillum huttiense gene for 16S rRNA, partial sequence, strain: NBRC 102521.	AB681855	1458	863372
124043	Herbaspirillum huttiense strain CIP 103296 16S ribosomal RNA gene, partial sequence.	EU024131	1317	86172

GC content

@ref	GC-content (mol%)
3877	62.0-62.5
67770	63.3

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Genomic insights into two new subspecies of Herbaspirillum huttiense strains isolated from diseased foliage in Florida.	Poudel M, Sharma A, Minsavage GV, Fullem K, Huguet-Tapia J, Norman DJ, Goss EM, Harmon CL, Jones JB.	Int J Syst Evol Microbiol	10.1099/ijsem.0.006597	2024
	Biochemical characteristics, adhesion, and cytotoxicity of environmental and clinical isolates of Herbaspirillum spp.	Marques AC, Paludo KS, Dallagassa CB, Surek M, Pedrosa FO, Souza EM, Cruz LM, LiPuma JJ, Zanata SM, Rego FG, Fadel-Picheth CM.	J Clin Microbiol	10.1128/jcm.02192-14	2015
Enzymology	Crystal structure of L-2-keto-3-deoxyfuconate 4-dehydrogenase reveals a unique binding mode as a alpha-furanosyl hemiketal of substrates.	Akagashi M, Watanabe S, Kwiatkowski S, Drozak J, Terawaki SI, Watanabe Y.	Sci Rep	10.1038/s41598-024-65627-8	2024
Metabolism	Novel non-phosphorylative pathway of pentose metabolism from bacteria.	Watanabe S, Fukumori F, Nishiwaki H, Sakurai Y, Tajima K, Watanabe Y.	Sci Rep	10.1038/s41598-018-36774-6	2019
Phylogeny	Reclassification of Herbaspirillum putei as a later heterotypic synonym of Herbaspirillum huttiense, with the description of H. huttiense subsp. huttiense subsp. nov. and H. huttiense subsp. putei subsp. nov., comb. nov., and description of Herbaspirillum aquaticum sp. nov.	Dobritsa AP, Reddy MCS, Samadpour M	Int J Syst Evol Microbiol	10.1099/ijs.0.009381-0	2009

References

#3877	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 10281
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#34240	; Curators of the CIP;
#47134	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 20333
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#119992	Collection of Institut Pasteur ; Curators of the CIP; CIP 103296
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Herbaspirillum huttiense subsp. putei (DSM 10281, ATCC 14670, JCM 21423)

Name and taxonomic classification

Morphology

Cell morphology

Pigmentation

Multimedia

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Observation

Metabolite utilization

Antibiotic resistance

Enzymes

API biotype100

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

16S sequences

GC content

Genome-based predictions

Literature

Literature

References

BacDive

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