Name: Herbaspirillum autotrophicum SA 32
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 11351

Type strain:

Species: Herbaspirillum autotrophicum

Strain Designation: SA 32, SA32

Culture col. no.: DSM 732, ATCC 29984, CCUG 12808, LMG 4326, CIP 107295, IAM 14942, NBRC 15327, JCM 21424, IFO 15327, VKM B-1394, UCCCB7

Strain history: CIP <- 2001, CCUG <- 1982, DSMZ <- M. Aragno: strain SA32

NCBI tax ID(s): 180195 (species)

SI-ID 891

LMG 4326, ATCC 29984, CCUG 12808, DSM 732, IFO 15327, VKM B-1394, IAM 14942, KCTC 2340, CIP 107295, NBRC 15327, JCM 21424

Other strains from Herbaspirillum autotrophicum

H. autotrophicum SA 33, CCUG 19112, LMG 7377, DSM ...

Section

Herbaspirillum autotrophicum SA 32 is an aerobe, mesophilic, motile bacterium that was isolated from eutrophic lake water.

motile
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Betaproteobacteria*
	Order	*Burkholderiales*
	Family	*Oxalobacteraceae*
	Genus	*Herbaspirillum*
	Species	**Herbaspirillum autotrophicum**
	Full Scientific Name (LPSN)	Herbaspirillum autotrophicum (Aragno and Schlegel 1978) Ding and Yokota 2004

Synonym

Aquaspirillum autotrophicum

Information on morphological and physiological properties Morphology

[Ref.: #116653]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.995

[Ref.: #116653]	Cell shape	rod-shaped

[Ref.: #116653]	Motility	no
[Ref.: #69480]	Motility	yes Confidence in %97.36

Information on culture and growth conditions Culture and growth conditions

[Ref.: #33759]

Culture medium

MEDIUM 14 - Williams and Rittenberg medium for Aquaspirillum genus

[Ref.: #33759]

Culture medium growth

[Ref.: #33759]

Culture medium composition

expand / minimize

[Ref.: #377]

Culture medium

MINERAL MEDIUM FOR CHEMOLITHOTROPHIC GROWTH (H-3) (DSMZ Medium 81)

[Ref.: #377]

Culture medium growth

[Ref.: #377]

Culture medium link

Medium recipe at MediaDive

[Ref.: #377]

Culture medium composition

expand / minimize

[Ref.: #377]

Culture medium

SPIRILLUM MEDIUM (DSMZ Medium 37)

[Ref.: #377]

Culture medium growth

[Ref.: #377]

Culture medium link

Medium recipe at MediaDive

[Ref.: #377]

Culture medium composition

expand / minimize

[Ref.: #116653]

Culture medium

CIP Medium 14

[Ref.: #116653]

Culture medium growth

[Ref.: #116653]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #377]		growth	28 °C
[Ref.: #33759]		growth	25 °C
[Ref.: #45831]		growth	25-30 °C
[Ref.: #67770]		growth	30 °C
[Ref.: #67772]		minimum	35 °C
[Ref.: #67772]		optimum	28 °C
[Ref.: #67772]		maximum	10 °C
[Ref.: #116653]		growth	15-30 °C
[Ref.: #116653]	no	growth	5 °C
[Ref.: #116653]	no	growth	37 °C
[Ref.: #116653]	no	growth	41 °C

[Ref.: #377]	Temperature range	mesophilic
[Ref.: #33759]	Temperature range	mesophilic
[Ref.: #45831]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #67772]	Temperature range	mesophilic
[Ref.: #67772]	Temperature range	psychrophilic
[Ref.: #116653]	Temperature range	psychrophilic
[Ref.: #116653]	Temperature range	mesophilic
[Ref.: #116653]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #45831]	Oxygen tolerance	aerobe
[Ref.: #116653]	Oxygen tolerance	obligate aerobe

[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.985

	Salt	Tested relation		Salt conc.
[Ref.: #116653]	NaCl		growth	0-2	%
[Ref.: #116653]	NaCl	no	growth	4	%
[Ref.: #116653]	NaCl	no	growth	6	%
[Ref.: #116653]	NaCl	no	growth	8	%
[Ref.: #116653]	NaCl	no	growth	10	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #116653]	citrate ChEBI	+	carbon source
[Ref.: #116653]	esculin ChEBI	-	hydrolysis
[Ref.: #116653]	glucose ChEBI	-	fermentation
[Ref.: #116653]	lactose ChEBI	-	fermentation
[Ref.: #116653]	nitrate ChEBI	-	reduction
[Ref.: #116653]	nitrate ChEBI	-	respiration
[Ref.: #116653]	nitrite ChEBI	-	reduction
[Ref.: #116653]	sodium thiosulfate ChEBI	-	builds gas from

	Antibiotica	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #116653]		0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)	no	yes

	Metabolite production	Metabolite	Production
[Ref.: #116653]		indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test	Methylred-test
[Ref.: #116653]		acetoin ChEBI	-
[Ref.: #116653]		glucose ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #116653]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #116653]	amylase	-
[Ref.: #116653]	beta-galactosidase	-	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #116653]	caseinase	-	3.4.21.50
[Ref.: #116653]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #116653]	DNase	-
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #116653]	gamma-glutamyltransferase	+	2.3.2.2
[Ref.: #116653]	gelatinase	-
[Ref.: #116653]	lecithinase	-
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #116653]	lipase	-
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #116653]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #116653]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #116653]	oxidase	+
[Ref.: #116653]	phenylalanine ammonia-lyase	-	4.3.1.24
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #116653]	tryptophan deaminase	-
[Ref.: #116653]	tween esterase	-
[Ref.: #116653]	urease	+	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Biotype100:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
[Ref.: #116653]	2138	not determinedn.d.	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	+	+	+	-	-	+	-	-	+	+	-	-	+	-	+	+	-	+	-	-	-	-	-	-	-	-	-	+	+	+	-	+	+	+	+	-	-	-	+	+	+	+	+	+	-	+	-	+	+	-	+

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #116653]	10239	-	-	+	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #377]	Sample type/isolated from	eutrophic lake water
[Ref.: #377]	Country	Switzerland
[Ref.: #377]	Country ISO 3 Code	CHE
[Ref.: #377]	Continent	Europe

[Ref.: #45831]	Sample type/isolated from	Eutrophic lake water
[Ref.: #45831]	Country	Switzerland
[Ref.: #45831]	Country ISO 3 Code	CHE
[Ref.: #45831]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Eutrophic lake water

[Ref.: #67772]	Sample type/isolated from	Eutrophic lake water
[Ref.: #67772]	Sampling date	1976
[Ref.: #67772]	Geographic location (country and/or sea, region)	Neuchâtel, Saint-Blaise
[Ref.: #67772]	Country	Switzerland
[Ref.: #67772]	Country ISO 3 Code	CHE
[Ref.: #67772]	Continent	Europe
[Ref.: #67772]	Geographic location	47.0167°/6.9833°

[Ref.: #116653]	Sample type/isolated from	Environment, Eutrophic lake water
[Ref.: #116653]	Country	Switzerland
[Ref.: #116653]	Country ISO 3 Code	CHE
[Ref.: #116653]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Environmental

#Aquatic

#Lake (large)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AB074524 (>99% sequence identity) for Herbaspirillum autotrophicum from Microbeatlas

Aquatic Samples	22
Soil Samples	6
Animal Samples	12
Plant Samples	13
Total Samples	53

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #377]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #67772]	Biosafety level	1
[Ref.: #116653]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #377]	Aquaspirillum autotrophicum gene for 16S rRNA, partial sequence	AB074524	1489	ENA	180195 tax ID
[Ref.: #20218]	Herbaspirillum autotrophicum gene for 16S rRNA, partial sequence, strain: NBRC 15327	AB680836	1458	ENA	180195 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Herbaspirillum autotrophicum IAM 14942	GCA_001189915	contig	GenBank	180195 tax ID
[Ref.: #66792]	Herbaspirillum autotrophicum strain IAM 14942	180195.5	wgs	PATRIC	180195 tax ID	*
[Ref.: #66792]	Herbaspirillum autotrophicum IAM 14942	2654587661	draft	JGI IMG/M	180195 tax ID	*

[Ref.: #377]

GC-content

61.7 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Herbaspirillum autotrophicum strain IAM 14942 PATRIC: 180195.5
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Herbaspirillum autotrophicum strain IAM 14942 PATRIC: 180195.5
[Ref.: #69480]	motile	yes	92.795	no
[Ref.: #69480]	gram-positive	no	98.876	no
[Ref.: #69480]	anaerobic	no	98.666	no
[Ref.: #69480]	aerobic	yes	93.001	yes
[Ref.: #69480]	halophile	no	93.652	no
[Ref.: #69480]	spore-forming	no	93.831	no
[Ref.: #69480]	glucose-util	yes	75.089	no
[Ref.: #69480]	flagellated	yes	66.438	no
[Ref.: #69480]	thermophile	no	99.394	yes
[Ref.: #69480]	glucose-ferment	no	88.795	no

Availability in culture collections External links

[Ref.: #377]

Culture collection no.

DSM 732, ATCC 29984, CCUG 12808, LMG 4326, CIP 107295, IAM 14942, NBRC 15327, JCM 21424, IFO 15327, VKM B-1394, UCCCB7

[Ref.: #80583]

SI-ID 891

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Paraherbaspirillum soli gen. nov., sp. nov. isolated from soil.	Anandham R, Kim SJ, Moon JY, Weon HY, Kwon SW	J Microbiol	10.1007/s12275-013-2563-5	2013	*
Enzymology	Localization and stability of hydrogenases from aerobic hydrogen bacteria.	Schneider K, Schlegel HG	Arch Microbiol	10.1007/BF00413086	1977	*

References

#377	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 732

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#33759 ; Curators of the CIP;
#45831 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 12808

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#67772 The University of Coimbra Bacteria Culture Collection (UCCCB) ; Curators of the UCCCB;

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#80583

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID891.1 )

#116653 Collection of Institut Pasteur ; Curators of the CIP; CIP 107295
* These data were automatically processed and therefore are not curated

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Herbaspirillum autotrophicum strain IAM 14942

Herbaspirillum autotrophicum strain IAM 14942

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