Evansella clarkii (DSM 8720, ATCC 700162, PN 102)

Name: Evansella clarkii (DSM 8720, ATCC 700162, PN 102)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 1132

Type strain

Strain Designation PN 102, VP 395

Culture col. no. DSM 8720 ATCC 700162 PN 102 VP 395 CIP 105301

NCBI tax ID(s) 79879

Special Collections DSMZ CIP

History <- H. Outtrup, Novo Nordisk A/S; VP 395 CIP <- 1997, DSMZ <- H. Outtrup, Novo Nordisk A/S: strain PN 102

Links

Evansella clarkii PN 102 is an obligate aerobe, Gram-positive, motile bacterium of the family Bacillaceae.

Gram-positive motile rod-shaped obligate aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 10537

LMG 17947,DSM 8720,ATCC 700162,CIP 105301,KCTC 3965,NRRL B-23344,CGMCC 1.3674

@ref 20215

Last LPSN update 2026-02-09 11:18:02

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Caryophanales

Family Bacillaceae

Genus Evansella

Species Evansella clarkii

Full scientific name Evansella clarkii (Nielsen et al. 1995) Gupta et al. 2020

Synonyms (1)

Bacillus clarkii

BacDive ID	Other strains from **Evansella clarkii** (2)	Type strain
1133	E. clarkii PN 103, AC 66, DSM 9725
1134	E. clarkii PN 104, K 51, DSM 9726

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility
118306	positive	rod-shaped

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
3250	ALKALINE NUTRIENT AGAR (DSMZ Medium 31)	Medium recipe at MediaDive	Name: ALKALINE NUTRIENT AGAR (DSMZ Medium 31; with strain-specific modifications) Composition: Agar 15.0 g/l Na2CO3 anhydrous 5.3 g/l Peptone 5.0 g/l NaCl 5.0 g/l NaHCO3 4.2 g/l Meat extract 3.0 g/l Distilled water
35806	MEDIUM 113 - for Bacillus		Distilled water make up to (900.000 ml);Columbia agar (39.000 g);Solution 1 - M00343 (100.000 ml)
118306	CIP Medium 113	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
3250	positive	growth	30
35806	positive	growth	30
118306	positive	growth	22-45
118306	negative	growth	10
118306	negative	growth	55

Culture pH

@ref	Ability	Type	PH
118306		growth	6

Physiology and metabolism

Oxygen tolerance

118306

Oxygen toleranceobligate aerobe

Spore formation

@ref	Spore formation	Confidence
125439		94.7

Halophily

@ref	Salt	Growth	Tested relation	Concentration
118306	NaCl	positive	growth	0-10 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68371	18305 ChEBI	arbutin	+	builds acid from	from API 50CH acid
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
68371	15824 ChEBI	D-fructose	+	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	12936 ChEBI	D-galactose	-	builds acid from	from API 50CH acid
68371	17634 ChEBI	D-glucose	+	builds acid from	from API 50CH acid
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68371	16899 ChEBI	D-mannitol	-	builds acid from	from API 50CH acid
68371	16024 ChEBI	D-mannose	+	builds acid from	from API 50CH acid
68371	16988 ChEBI	D-ribose	+	builds acid from	from API 50CH acid
68371	17924 ChEBI	D-sorbitol	-	builds acid from	from API 50CH acid
68371	16443 ChEBI	D-tagatose	-	builds acid from	from API 50CH acid
68371	65327 ChEBI	D-xylose	-	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
68371	4853 ChEBI	esculin	+	builds acid from	from API 50CH acid
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
68371	28066 ChEBI	gentiobiose	-	builds acid from	from API 50CH acid
68371	17754 ChEBI	glycerol	-	builds acid from	from API 50CH acid
68371	28087 ChEBI	glycogen	+	builds acid from	from API 50CH acid
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
68371	30849 ChEBI	L-arabinose	-	builds acid from	from API 50CH acid
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	-	builds acid from	from API 50CH acid
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
68371	17716 ChEBI	lactose	-	builds acid from	from API 50CH acid
68371	17306 ChEBI	maltose	+	builds acid from	from API 50CH acid
68371	6731 ChEBI	melezitose	-	builds acid from	from API 50CH acid
68371	28053 ChEBI	melibiose	-	builds acid from	from API 50CH acid
68371	320061 ChEBI	methyl alpha-D-glucopyranoside	-	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
68371	59640 ChEBI	N-acetylglucosamine	+	builds acid from	from API 50CH acid
118306	17632 ChEBI	nitrate	+	reduction
118306	17632 ChEBI	nitrate	-	respiration
118306	16301 ChEBI	nitrite	+	reduction
68371		Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371		Potassium 5-ketogluconate	-	builds acid from	from API 50CH acid
68371	16634 ChEBI	raffinose	-	builds acid from	from API 50CH acid
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
68371	17814 ChEBI	salicin	+	builds acid from	from API 50CH acid
68371	28017 ChEBI	starch	+	builds acid from	from API 50CH acid
68371	17992 ChEBI	sucrose	-	builds acid from	from API 50CH acid
68371	27082 ChEBI	trehalose	+	builds acid from	from API 50CH acid
68371	32528 ChEBI	turanose	-	builds acid from	from API 50CH acid
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

Metabolite production

@ref	Chebi-ID	Metabolite	Production
118306	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Methylred-test
118306	15688 ChEBI	acetoin	+
118306	17234 ChEBI	glucose		+

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
118306	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	+	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	+	3.2.1.23	from API zym
118306	beta-galactosidase	+	3.2.1.23
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
118306	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
118306	gamma-glutamyltransferase	+	2.3.2.2
118306	gelatinase	-
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
118306	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
118306	ornithine decarboxylase	-	4.1.1.17
118306	oxidase	-
118306	phenylalanine ammonia-lyase	-	4.3.1.24
68382	trypsin	-	3.4.21.4	from API zym
118306	urease	-	3.5.1.5
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
118306	-	+	+	+	-	+	-	-	-	+	+	+	-	+	-	-	-	-	-	-

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
118306	not determinedn.d.	-	-	-	-	+	-	-	-	-	-	+	+	+	-	-	-	-	-	-	-	-	+	+/-	+	+	+	+/-	+	-	-	-	+	-	-	-	+	+	-	-	-	-	-	-	-	-	-	+/-	-	-

Isolation, sampling and environmental information

Taxonmaps

69479

Global distribution of 16S sequence X76444 (>99% sequence identity) for Bacillales from Microbeatlas

Aquatic Samples	68
Soil Samples	122
Animal Samples	416
Plant Samples	51
Total Samples	657

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
3250	1	Risk group (German classification) According to TRBA 466
118306	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM201969v1 assembly for Evansella clarkii DSM 8720	scaffold	GCA_002019695	79879.3	2898074128	79879	70.94

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	Bacillus clarkii DSM 8720, 16S rRNA gene	X76444	1522		79879

GC content

@ref	GC-content (mol%)	Method
3250	42.4	high performance liquid chromatography (HPLC)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Evansella clarkii DSM 8720
NCBI: GCA_002019695

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	94.70	no
125439	motility	BacteriaNetⓘ	yes	86.90	no
125439	gram_stain	BacteriaNetⓘ	variable	86.80	no
125439	oxygen_tolerance	BacteriaNetⓘ	facultative anaerobe	87.70	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Evansella clarkii DSM 8720
NCBI: GCA_002019695.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	72.05	no
125438	anaerobic	anaerobicⓘ	no	96.32	yes
125438	aerobic	aerobicⓘ	yes	74.36	yes
125438	spore-forming	spore-formingⓘ	yes	88.07	no
125438	thermophilic	thermophileⓘ	no	91.07	yes
125438	flagellated	motile2+ⓘ	yes	80.52	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	H⁺-capacitor and ATP production in obligate alkaliphilic Bacillaceae: insights into cytochrome c and H⁺ transport mechanisms.	Yumoto I.	Front Microbiol	10.3389/fmicb.2025.1637315	2025
Metabolism	Contribution of intracellular negative ion capacity to Donnan effect across the membrane in alkaliphilic Bacillus spp.	Goto T, Hirabayashi T, Morimoto H, Yamazaki K, Inoue N, Matsuyama H, Yumoto I	J Bioenerg Biomembr	10.1007/s10863-015-9641-9	2016
	Relationship between rates of respiratory proton extrusion and ATP synthesis in obligately alkaliphilic Bacillus clarkii DSM 8720(T).	Hirabayashi T, Goto T, Morimoto H, Yoshimune K, Matsuyama H, Yumoto I	J Bioenerg Biomembr	10.1007/s10863-012-9430-7	2012
Phylogeny	Bacillus populi sp. nov. isolated from Populus euphratica rhizosphere soil of the Taklamakan desert.	Liu B, Liu GH, Wang XY, Wang JP, Zhu YJ, Zhang HF, Sengonca C	Int J Syst Evol Microbiol	10.1099/ijsem.0.002476	2017
Phylogeny	Bacillus polygoni sp. nov., a moderately halophilic, non-motile obligate alkaliphile isolated from indigo balls.	Aino K, Hirota K, Matsuno T, Morita N, Nodasaka Y, Fujiwara T, Matsuyama H, Yoshimune K, Yumoto I	Int J Syst Evol Microbiol	10.1099/ijs.0.65193-0	2008
Phylogeny	Bacillus taeanensis sp. nov., a halophilic Gram-positive bacterium from a solar saltern in Korea.	Lim JM, Jeon CO, Kim CJ	Int J Syst Evol Microbiol	10.1099/ijs.0.64036-0	2006

References

#3250	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 8720
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#35806	; Curators of the CIP;
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#68371	Automatically annotated from API 50CH acid .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#118306	Collection of Institut Pasteur ; Curators of the CIP; CIP 105301
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Evansella clarkii (DSM 8720, ATCC 700162, PN 102)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API zym

API 50CHac

Isolation, sampling and environmental information

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_002019695

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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