Name: Evansella clarkii PN 102
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 1132

Type strain:

Species: Evansella clarkii

Strain Designation: PN 102, VP 395

Culture col. no.: DSM 8720, ATCC 700162, CIP 105301

Strain history: CIP <- 1997, DSMZ <- H. Outtrup, Novo Nordisk A/S: strain PN 102

NCBI tax ID(s): 79879 (species)

SI-ID 10537

LMG 17947, DSM 8720, ATCC 700162, CIP 105301, KCTC 3965, NRRL B-23344, CGMCC 1.3674

Other strains from Evansella clarkii

E. clarkii PN 103, AC 66, DSM 9725
E. clarkii PN 104, K 51, DSM 9726

Section

Evansella clarkii PN 102 is an obligate aerobe, spore-forming, mesophilic bacterium of the family Bacillaceae.

motile
spore-forming
mesophilic
obligate aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Evansella*
	Species	**Evansella clarkii**
	Full Scientific Name (LPSN)	Evansella clarkii (Nielsen et al. 1995) Gupta et al. 2020

Synonym

Bacillus clarkii

Information on morphological and physiological properties Morphology

[Ref.: #118306]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #118306]	Cell shape	rod-shaped

[Ref.: #118306]	Motility	yes
[Ref.: #69480]	Motility	yes Confidence in %97.191

Information on culture and growth conditions Culture and growth conditions

[Ref.: #3250]

Culture medium

ALKALINE NUTRIENT AGAR (DSMZ Medium 31)

[Ref.: #3250]

Culture medium growth

[Ref.: #3250]

Culture medium link

Medium recipe at MediaDive

[Ref.: #3250]

Culture medium composition

expand / minimize

[Ref.: #35806]

Culture medium

MEDIUM 113 - for Bacillus

[Ref.: #35806]

Culture medium growth

[Ref.: #35806]

Culture medium composition

expand / minimize

[Ref.: #118306]

Culture medium

CIP Medium 113

[Ref.: #118306]

Culture medium growth

[Ref.: #118306]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #3250]		growth	30 °C
[Ref.: #35806]		growth	30 °C
[Ref.: #118306]		growth	22-45 °C
[Ref.: #118306]	no	growth	10 °C
[Ref.: #118306]	no	growth	55 °C

[Ref.: #3250]	Temperature range	mesophilic
[Ref.: #35806]	Temperature range	mesophilic
[Ref.: #118306]	Temperature range	psychrophilic
[Ref.: #118306]	Temperature range	thermophilic

	pH	Kind of pH		pH
[Ref.: #118306]		no	growth	6

Information on physiology and metabolism Physiology and metabolism

[Ref.: #118306]

Oxygen tolerance

obligate aerobe

[Ref.: #69481]	Ability of spore formation	yes Confidence in %100
[Ref.: #69480]	Ability of spore formation	yes Confidence in %99.674

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #118306]		NaCl		growth	0-10	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68371]	arbutin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fructose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-galactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-glucose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-ribose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	esculin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gentiobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycerol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycogen ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	lactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	maltose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	melezitose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melibiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	N-acetylglucosamine ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #118306]	nitrate ChEBI	+	reduction
[Ref.: #118306]	nitrate ChEBI	-	respiration
[Ref.: #118306]	nitrite ChEBI	+	reduction
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	raffinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	salicin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	starch ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	sucrose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	trehalose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	turanose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #118306]		indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test	Methylred-test
[Ref.: #118306]		acetoin ChEBI	+
[Ref.: #118306]		glucose ChEBI		+

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #118306]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	+	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #118306]	beta-galactosidase	+	3.2.1.23
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #118306]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #118306]	gamma-glutamyltransferase	+	2.3.2.2
[Ref.: #118306]	gelatinase	-
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #118306]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #118306]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #118306]	oxidase	-
[Ref.: #118306]	phenylalanine ammonia-lyase	-	4.3.1.24
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #118306]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #118306]	10026	not determinedn.d.	-	-	-	-	+	-	-	-	-	-	+	+	+	-	-	-	-	-	-	-	-	+	+/-	+	+	+	+/-	+	-	-	-	+	-	-	-	+	+	-	-	-	-	-	-	-	-	-	+/-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #118306]	8598	-	+	+	+	-	+	-	-	-	+	+	+	-	+	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #69479]

Global distribution of 16S sequence X76444 (>99% sequence identity) for Bacillales from Microbeatlas

Aquatic Samples	68
Soil Samples	122
Animal Samples	416
Plant Samples	51
Total Samples	657

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #3250]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #118306]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Bacillus clarkii DSM 8720, 16S rRNA gene	X76444	1522	ENA	79879 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Evansella clarkii DSM 8720	GCA_002019695	scaffold	GenBank	79879 tax ID	*
[Ref.: #66792]	[Bacillus] clarkii strain DSM 8720	79879.3	wgs	PATRIC	79879 tax ID	*
[Ref.: #66792]	Bacillus clarkii DSM 8720	2898074128	draft	JGI IMG/M	79879 tax ID	*

[Ref.: #3250]

GC-content

42.4 mol%

high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Evansella clarkii DSM 8720 NCBI: GCA_002019695.1
[Ref.: #69481]	spore-forming	yes	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Evansella clarkii DSM 8720 NCBI: GCA_002019695.1
[Ref.: #69480]	flagellated	yes	69.45	no
[Ref.: #69480]	gram-positive	yes	88.058	no
[Ref.: #69480]	anaerobic	no	97.979	no
[Ref.: #69480]	halophile	yes	87.636	no
[Ref.: #69480]	spore-forming	yes	91.665	no
[Ref.: #69480]	glucose-util	yes	89.919	no
[Ref.: #69480]	aerobic	yes	82.658	no
[Ref.: #69480]	thermophile	no	93.064	no
[Ref.: #69480]	motile	yes	86.453	no
[Ref.: #69480]	glucose-ferment	no	94.548	no

Availability in culture collections External links

[Ref.: #3250]

Culture collection no.

DSM 8720, ATCC 700162, CIP 105301

[Ref.: #70803]

SI-ID 10537

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Bacillus populi sp. nov. isolated from Populus euphratica rhizosphere soil of the Taklamakan desert.	Liu B, Liu GH, Wang XY, Wang JP, Zhu YJ, Zhang HF, Sengonca C	Int J Syst Evol Microbiol	10.1099/ijsem.0.002476	2017	*
Metabolism	Contribution of intracellular negative ion capacity to Donnan effect across the membrane in alkaliphilic Bacillus spp.	Goto T, Hirabayashi T, Morimoto H, Yamazaki K, Inoue N, Matsuyama H, Yumoto I	J Bioenerg Biomembr	10.1007/s10863-015-9641-9	2016	*
Phylogeny	Bacillus polygoni sp. nov., a moderately halophilic, non-motile obligate alkaliphile isolated from indigo balls.	Aino K, Hirota K, Matsuno T, Morita N, Nodasaka Y, Fujiwara T, Matsuyama H, Yoshimune K, Yumoto I	Int J Syst Evol Microbiol	10.1099/ijs.0.65193-0	2008	*
Phylogeny	Bacillus taeanensis sp. nov., a halophilic Gram-positive bacterium from a solar saltern in Korea.	Lim JM, Jeon CO, Kim CJ	Int J Syst Evol Microbiol	10.1099/ijs.0.64036-0	2006	*
	Relationship between rates of respiratory proton extrusion and ATP synthesis in obligately alkaliphilic Bacillus clarkii DSM 8720(T).	Hirabayashi T, Goto T, Morimoto H, Yoshimune K, Matsuyama H, Yumoto I	J Bioenerg Biomembr	10.1007/s10863-012-9430-7	2012	*

References

#3250

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 8720

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#35806 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68371

Automatically annotated from API 50CH acid .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#70803

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID10537.1 )

#118306 Collection of Institut Pasteur ; Curators of the CIP; CIP 105301
* These data were automatically processed and therefore are not curated

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Evansella clarkii DSM 8720

Evansella clarkii DSM 8720

Change proposal

Change proposal