Shouchella clausii (DSM 8716, ATCC 700160, CIP 104718)

Name: Shouchella clausii (DSM 8716, ATCC 700160, CIP 104718)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 1127

Type strain

Strain Designation PN 23, C 360

Culture col. no. DSM 8716 ATCC 700160 CIP 104718 NCIB 10309 PN 23 4 more

NCBI tax ID(s) 79880

Special Collections DSMZ CCUG CIP

History DSMZ <- NCIMB <- Novo Industri; C 360 CIP <- 1996, DSM <- NCIMB <- Novo Industri.: strain C 360 = PN 23

Links

Shouchella clausii PN 23 is an aerobe, spore-forming, Gram-positive bacterium that was isolated from garden soil.

spore-forming Gram-positive motile rod-shaped aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 14017

LMG 17945,CIP 104718,DSM 8716,NCIMB 10309,ATCC 700160,NCIB 10309,CCUG 47262,KCTC 3825,KACC 10918,NRRL B-23342,CGMCC 1.3672

@ref 20215

Last LPSN update 2026-05-29 08:25:11

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Caryophanales

Family Bacillaceae

Genus Shouchella

Species Shouchella clausii

Full scientific name Shouchella clausii (Nielsen et al. 1995) Joshi et al. 2022

Synonyms (5)

Alkalihalobacillus clausii
Shouchella rhizosphaerae
Bacillus rhizosphaerae
Bacillus clausii
Alkalihalobacillus rhizosphaerae

BacDive ID	Other strains from **Shouchella clausii** (9)	Type strain
1124	S. clausii PN 43, 221, DSM 2512, ATCC 21522, FERM P-316, ...
1125	S. clausii PN 46, Y-76, DSM 2515, ATCC 21537, FERM P-569, ...
1126	S. clausii M-29, DSM 2525, ATCC 31084, FERM P-2296
1128	S. clausii PN 24, C 372, DSM 9783, NCIMB 10317
1129	S. clausii PN 35, C 374, DSM 9784, NCIMB 10319
1130	S. clausii PN 76, Br-F, DSM 9785
1131	S. clausii K2-6, DSM 30907
1314	S. clausii SC-N012, DSM 21911, NCCB 100267
138413	S. clausii CIP 51.33

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility
118307	positive	rod-shaped

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
3246	ALKALINE NUTRIENT AGAR (DSMZ Medium 31)	Medium recipe at MediaDive	Name: ALKALINE NUTRIENT AGAR (DSMZ Medium 31; with strain-specific modifications) Composition: Agar 15.0 g/l Na2CO3 anhydrous 5.3 g/l Peptone 5.0 g/l NaCl 5.0 g/l NaHCO3 4.2 g/l Meat extract 3.0 g/l Distilled water
35723	MEDIUM 3 - Columbia agar		Columbia agar (39.000 g);distilled water (1000.000 ml)
118307	CIP Medium 3	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
3246	positive	growth	30
35723	positive	growth	30
57609	positive	growth	30-37
118307	positive	growth	10-45
118307	negative	growth	55

Culture pH

@ref	Ability	Type	PH
118307	positive	growth	6

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
57609	aerobe
118307	obligate aerobe
125439	obligate aerobe	95.861

Spore formation

@ref	Spore formation	Confidence
118307
125439		90.045
125438		93.548

Halophily

@ref	Salt	Growth	Tested relation	Concentration
118307	NaCl	positive	growth	0-8 %
118307	NaCl		growth	10 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68371	27613 ChEBI	amygdalin	-	builds acid from	from API 50CH acid
68371	18305 ChEBI	arbutin	-	builds acid from	from API 50CH acid
68371	17057 ChEBI	cellobiose	-	builds acid from	from API 50CH acid
118307	16947 ChEBI	citrate	-	carbon source
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
68371	15824 ChEBI	D-fructose	-	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	12936 ChEBI	D-galactose	-	builds acid from	from API 50CH acid
68371	17634 ChEBI	D-glucose	-	builds acid from	from API 50CH acid
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68371	16899 ChEBI	D-mannitol	-	builds acid from	from API 50CH acid
68371	16024 ChEBI	D-mannose	-	builds acid from	from API 50CH acid
68371	16988 ChEBI	D-ribose	-	builds acid from	from API 50CH acid
68371	17924 ChEBI	D-sorbitol	-	builds acid from	from API 50CH acid
68371	16443 ChEBI	D-tagatose	-	builds acid from	from API 50CH acid
68371	65327 ChEBI	D-xylose	-	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
68371	4853 ChEBI	esculin	+	builds acid from	from API 50CH acid
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
68371	28066 ChEBI	gentiobiose	-	builds acid from	from API 50CH acid
68371	17754 ChEBI	glycerol	-	builds acid from	from API 50CH acid
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
68371	30849 ChEBI	L-arabinose	-	builds acid from	from API 50CH acid
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	-	builds acid from	from API 50CH acid
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
68371	17716 ChEBI	lactose	-	builds acid from	from API 50CH acid
68371	17306 ChEBI	maltose	-	builds acid from	from API 50CH acid
68371	6731 ChEBI	melezitose	-	builds acid from	from API 50CH acid
68371	28053 ChEBI	melibiose	-	builds acid from	from API 50CH acid
68371	320061 ChEBI	methyl alpha-D-glucopyranoside	-	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
68371	59640 ChEBI	N-acetylglucosamine	-	builds acid from	from API 50CH acid
118307	17632 ChEBI	nitrate	+	reduction
118307	17632 ChEBI	nitrate	-	respiration
118307	16301 ChEBI	nitrite	-	reduction
68371	0 ChEBI	Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371	16634 ChEBI	raffinose	-	builds acid from	from API 50CH acid
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
68371	17814 ChEBI	salicin	-	builds acid from	from API 50CH acid
68371	28017 ChEBI	starch	-	builds acid from	from API 50CH acid
68371	17992 ChEBI	sucrose	-	builds acid from	from API 50CH acid
68371	27082 ChEBI	trehalose	-	builds acid from	from API 50CH acid
68371	32528 ChEBI	turanose	-	builds acid from	from API 50CH acid
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

Metabolite production

@ref	Chebi-ID	Metabolite	Production
118307	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test
118307	15688 ChEBI	acetoin	-

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
118307	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
118307	amylase	-
68382	beta-galactosidase	+	3.2.1.23	from API zym
68382	beta-glucosidase	+	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
118307	caseinase	+	3.4.21.50
118307	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
118307	DNase	+
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
118307	gelatinase	+
118307	lecithinase	-
68382	leucine arylamidase	-	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
118307	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
118307	ornithine decarboxylase	-	4.1.1.17
118307	oxidase	+
68382	trypsin	-	3.4.21.4	from API zym
118307	urease	-	3.5.1.5
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
118307	-	+	+	+	-	-	-	-	-	-	+	+	-	+	-	+	+	-	-	-

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
118307	not determinedn.d.	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	+/-	-	-	-	-	-	-	-	-	-	+/-	-	+/-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Soil
#Engineered	#Agriculture	#Garden

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
3246	garden soil	Slagelse	Denmark	DNK	Europe
57609	Garden soil
118307	Environment, Garden soil

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
3246	1	Risk group (German classification) According to TRBA 466
118307	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
124043	ASM3447930v1 assembly for Shouchella clausii ATCC 700160	complete	GCA_034479305	79880.155		79880	99.04
66792	ASM225011v1 assembly for Shouchella clausii DSM 8716	complete	GCA_002250115	79880.14	2811995369	79880	98

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	Bacillus clausii DSM 8716, 16S rRNA gene	X76440	1505		79880
124043	Alkalihalobacillus clausii strain DSM 8716 16S ribosomal RNA gene, partial sequence.	MW198156	601		79880

GC content

@ref	GC-content (mol%)	Method
3246	42.8	high performance liquid chromatography (HPLC)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM3447930v1 assembly for Shouchella clausii ATCC 700160
NCBI: GCA_034479305

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	95.86	no
125439	gram_stain	BacteriaNetⓘ	variable	74.86	no
125439	motility	BacteriaNetⓘ	yes	81.62	no
125439	spore_formation	BacteriaNetⓘ	yes	90.05	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Alkalihalobacillus clausii DSM 8716
NCBI: GCA_002250115.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	76.75	no
125438	anaerobic	anaerobicⓘ	no	93.06	no
125438	aerobic	aerobicⓘ	yes	75.14	yes
125438	spore-forming	spore-formingⓘ	yes	93.55	no
125438	thermophilic	thermophileⓘ	no	89.99	no
125438	flagellated	motile2+ⓘ	yes	81.21	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Genetics	Strain-Dependent Adhesion Variations of Shouchella clausii Isolated from Healthy Human Volunteers: A Study on Cell Surface Properties and Potential Probiotic Benefits.	Dhakephalkar T, Pisu V, Margale P, Chandras S, Shetty D, Wagh S, Dagar SS, Kapse N, Dhakephalkar PK.	Microorganisms	10.3390/microorganisms12091771	2024
	Evaluating the Compositional Quality of Probiotics Containing Bacillus clausii in India.	Kesavelu D, Ganpathy S, Jog P, Acharya B, Saxena V, Telang N, Gaikwad N.	Adv Ther	10.1007/s12325-025-03289-2	2025
	Characterization and In Vitro Probiotic Assessment of Alkalihalobacillus wakoensis as New Potent Probiotic Candidate.	Zarei A, Gholami A, Kanannejad Z, Berenjian A, Nabavizadeh SH, Mohkam M.	Food Sci Nutr	10.1002/fsn3.71031	2025
Genetics	The Resistance Abilities of Some Bacillus Species to Gastrointestinal Tract Conditions: Whole Genome Sequencing of the Novel Candidate Probiotic Strains Bacillus clausiiBA8 and Bacillus subtilisBA11.	Soylemez-Milli N, Erturkmen P, Alp Baltakesmez D.	Food Sci Nutr	10.1002/fsn3.70018	2025
	Effect of Physical and Chemical Treatments on Viability, Sub-Lethal Injury, and Release of Cellular Components from Bacillus clausii and Bacillus coagulans Spores and Cells.	Bevilacqua A, Petruzzi L, Sinigaglia M, Speranza B, Campaniello D, Ciuffreda E, Corbo MR	Foods	10.3390/foods9121814	2020
Enzymology	Synthesis of alpha-hydroxy ketones and vicinal (R,R)-diols by Bacillus clausii DSM 8716(T) butanediol dehydrogenase.	Muschallik L, Molinnus D, Jablonski M, Kipp CR, Bongaerts J, Pohl M, Wagner T, Schoning MJ, Selmer T, Siegert P	RSC Adv	10.1039/d0ra02066d	2020
Phylogeny	Genome-based reclassification of Bacillus plakortidis Borchert et al. 2007 and Bacillus lehensis Ghosh et al. 2007 as a later heterotypic synonym of Bacillus oshimensis Yumoto et al. 2005; Bacillus rhizosphaerae Madhaiyan et al. 2011 as a later heterotypic synonym of Bacillus clausii Nielsen et al. 1995.	Liu GH, Narsing Rao MP, Dong ZY, Wang JP, Che JM, Chen QQ, Sengonca C, Liu B, Li WJ	Antonie Van Leeuwenhoek	10.1007/s10482-019-01299-z	2019
Enzymology	(R,R)-Butane-2,3-diol dehydrogenase from Bacillus clausii DSM 8716(T): Cloning and expression of the bdhA-gene, and initial characterization of enzyme.	Muschallik L, Molinnus D, Bongaerts J, Pohl M, Wagner T, Schoning MJ, Siegert P, Selmer T	J Biotechnol	10.1016/j.jbiotec.2017.07.020	2017
	Probiotic characterization of Bacillus subtilis P223 isolated from kimchi.	Jeon HL, Lee NK, Yang SJ, Kim WS, Paik HD	Food Sci Biotechnol	10.1007/s10068-017-0148-5	2017
Biotechnology	Tolerance to challenges miming gastrointestinal transit by spores and vegetative cells of Bacillus clausii.	Cenci G, Trotta F, Caldini G	J Appl Microbiol	10.1111/j.1365-2672.2006.03042.x	2006
Genetics	Chromosomal aadD2 encodes an aminoglycoside nucleotidyltransferase in Bacillus clausii.	Bozdogan B, Galopin S, Gerbaud G, Courvalin P, Leclercq R	Antimicrob Agents Chemother	10.1128/AAC.47.4.1343-1346.2003	2003
Pathogenicity	Characterization of a new erm-related macrolide resistance gene present in probiotic strains of Bacillus clausii.	Bozdogan B, Galopin S, Leclercq R	Appl Environ Microbiol	10.1128/AEM.70.1.280-284.2004	2004
	Shouchella tritolerans sp. nov., a facultative anaerobic bacterium isolated from marine sediments.	Xie CJ, Yang S, Han S, Liu GH, Zhou SG.	Arch Microbiol	10.1007/s00203-023-03474-3	2023
	Alkalihalobacterium elongatum gen. nov. sp. nov.: An Antibiotic-Producing Bacterium Isolated From Lonar Lake and Reclassification of the Genus Alkalihalobacillus Into Seven Novel Genera.	Joshi A, Thite S, Karodi P, Joseph N, Lodha T.	Front Microbiol	10.3389/fmicb.2021.722369	2021
Phylogeny	Bacillus shacheensis sp. nov., a moderately halophilic bacterium isolated from a saline-alkali soil.	Lei Z, Qiu P, Ye R, Tian J, Liu Y, Wang L, Tang SK, Li WJ, Tian Y	J Gen Appl Microbiol	DN/JST.JSTAGE/jgam/60.101	2014
Phylogeny	Bacillus rhizosphaerae sp. nov., an novel diazotrophic bacterium isolated from sugarcane rhizosphere soil.	Madhaiyan M, Poonguzhali S, Lee JS, Lee KC, Hari K	Antonie Van Leeuwenhoek	10.1007/s10482-011-9600-3	2011

References

#3246	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 8716
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#35723	; Curators of the CIP;
#57609	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 47262
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#68371	Automatically annotated from API 50CH acid .
#68382	Automatically annotated from API zym .
#118307	Collection of Institut Pasteur ; Curators of the CIP; CIP 104718
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Shouchella clausii (DSM 8716, ATCC 700160, CIP 104718)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API zym

API 50CHac

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_034479305

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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