Name: Shouchella clausii PN 23
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 1127

Type strain:

Species: Shouchella clausii

Strain Designation: PN 23, C 360

Culture col. no.: DSM 8716, ATCC 700160, CIP 104718, NCIB 10309, CCUG 47262, NCIMB 10309, LMG 17945

Strain history: CIP <- 1996, DSM <- NCIMB <- Novo Industri.: strain C 360 = PN 23

NCBI tax ID(s): 79880 (species)

SI-ID 14017

LMG 17945, CIP 104718, DSM 8716, NCIMB 10309, ATCC 700160, NCIB 10309, CCUG 47262, KCTC 3825, KACC 10918, NRRL B-23342, CGMCC 1.3672

Special Collections

Other strains from Shouchella clausii

Section

Shouchella clausii PN 23 is an aerobe, spore-forming, motile bacterium that was isolated from garden soil.

motile
spore-forming
aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Shouchella*
	Species	**Shouchella clausii**
	Full Scientific Name (LPSN)	Shouchella clausii (Nielsen et al. 1995) Joshi et al. 2022

	Synonym	Alkalihalobacillus clausii
	Synonym	Bacillus clausii
	Synonym	Shouchella rhizosphaerae
	Synonym	Alkalihalobacillus rhizosphaerae
	Synonym	Bacillus rhizosphaerae

Information on morphological and physiological properties Morphology

[Ref.: #118307]	Gram stain	positive

[Ref.: #118307]	Cell shape	rod-shaped

[Ref.: #118307]	Motility	yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #3246]

Culture medium

ALKALINE NUTRIENT AGAR (DSMZ Medium 31)

[Ref.: #3246]

Culture medium growth

[Ref.: #3246]

Culture medium link

Medium recipe at MediaDive

[Ref.: #3246]

Culture medium composition

expand / minimize

[Ref.: #35723]

Culture medium

MEDIUM 3 - Columbia agar

[Ref.: #35723]

Culture medium growth

[Ref.: #35723]

Culture medium composition

expand / minimize

[Ref.: #118307]

Culture medium

CIP Medium 3

[Ref.: #118307]

Culture medium growth

[Ref.: #118307]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #3246]		growth	30 °C
[Ref.: #35723]		growth	30 °C
[Ref.: #57609]		growth	30-37 °C
[Ref.: #118307]		growth	10-45 °C
[Ref.: #118307]	no	growth	55 °C

	pH	Kind of pH		pH
[Ref.: #118307]			growth	6

Information on physiology and metabolism Physiology and metabolism

[Ref.: #57609]	Oxygen tolerance	aerobe
[Ref.: #118307]	Oxygen tolerance	obligate aerobe

[Ref.: #118307]	Ability of spore formation	yes
[Ref.: #69480]	Ability of spore formation	yes Confidence in %93.548
[Ref.: #69481]	Ability of spore formation	yes Confidence in %100

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #118307]		NaCl		growth	0-8	%
[Ref.: #118307]		NaCl	no	growth	10	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68371]	amygdalin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	arbutin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	cellobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #118307]	citrate ChEBI	-	carbon source
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fructose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-galactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-glucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-ribose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	esculin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gentiobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycerol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	lactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	maltose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melezitose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melibiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	N-acetylglucosamine ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #118307]	nitrate ChEBI	+	reduction
[Ref.: #118307]	nitrate ChEBI	-	respiration
[Ref.: #118307]	nitrite ChEBI	-	reduction
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	raffinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	salicin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	starch ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	sucrose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	trehalose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	turanose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #118307]		indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #118307]		acetoin ChEBI	-

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #118307]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #118307]	amylase	-
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #118307]	caseinase	+	3.4.21.50
[Ref.: #118307]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #118307]	DNase	+
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #118307]	gelatinase	+
[Ref.: #118307]	lecithinase	-
[Ref.: #68382]	leucine arylamidase	-	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #118307]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #118307]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #118307]	oxidase	+
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #118307]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #118307]	9716	not determinedn.d.	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	+/-	-	-	-	-	-	-	-	-	-	+/-	-	+/-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #118307]	8148	-	+	+	+	-	-	-	-	-	-	+	+	-	+	-	+	+	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #3246]	Sample type/isolated from	garden soil
[Ref.: #3246]	Geographic location (country and/or sea, region)	Slagelse
[Ref.: #3246]	Country	Denmark
[Ref.: #3246]	Country ISO 3 Code	DNK
[Ref.: #3246]	Continent	Europe

[Ref.: #57609]	Sample type/isolated from	Garden soil

[Ref.: #118307]	Sample type/isolated from	Environment, Garden soil
* marker position based on {}

Isolation sources categories

#Environmental	#Terrestrial	#Soil
#Engineered	#Agriculture	#Garden

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #3246]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #118307]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Bacillus clausii DSM 8716, 16S rRNA gene	X76440	1505	GenBank ENA	79880 tax ID	*
[Ref.: #124043]	Bacillus clausii partial 16S rRNA gene, strain DSM 8716, isolate a1, clone a1	LS992075	964	GenBank ENA	79880 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Shouchella clausii DSM 8716	GCA_002250115	complete	GenBank ENA	79880 tax ID	*
[Ref.: #66792]	Bacillus clausii strain DSM 8716	79880.14	complete	PATRIC	79880 tax ID	*
[Ref.: #66792]	Bacillus clausii DSM 8716	2811995369	complete	JGI IMG/M	79880 tax ID	*

[Ref.: #3246]

GC-content

42.8 mol%

high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Bacillus clausii strain DSM 8716 PATRIC: 79880.14
[Ref.: #69481]	spore-forming	spore-formingⓘ	yes	100	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Alkalihalobacillus clausii DSM 8716 NCBI: GCA_002250115.1
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	76.751	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	93.064	no
[Ref.: #69480]	aerobic	aerobicⓘ	yes	75.138	yes
[Ref.: #69480]	spore-forming	spore-formingⓘ	yes	93.548	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	89.99	no
[Ref.: #69480]	flagellated	motile2+ⓘ	yes	81.205	no

Availability in culture collections External links

[Ref.: #3246]

Culture collection no.

DSM 8716, ATCC 700160, CIP 104718, NCIB 10309, CCUG 47262, NCIMB 10309, LMG 17945

[Ref.: #70798]

SI-ID 14017

Literature:

Only first 10 entries are displayed. Click here to see all.

	Title	Authors	Journal	DOI	Year
Enzymology	Synthesis of alpha-hydroxy ketones and vicinal (R,R)-diols by Bacillus clausii DSM 8716(T) butanediol dehydrogenase.	Muschallik L, Molinnus D, Jablonski M, Kipp CR, Bongaerts J, Pohl M, Wagner T, Schoning MJ, Selmer T, Siegert P	RSC Adv	10.1039/d0ra02066d	2020	*
Phylogeny	Genome-based reclassification of Bacillus plakortidis Borchert et al. 2007 and Bacillus lehensis Ghosh et al. 2007 as a later heterotypic synonym of Bacillus oshimensis Yumoto et al. 2005; Bacillus rhizosphaerae Madhaiyan et al. 2011 as a later heterotypic synonym of Bacillus clausii Nielsen et al. 1995.	Liu GH, Narsing Rao MP, Dong ZY, Wang JP, Che JM, Chen QQ, Sengonca C, Liu B, Li WJ	Antonie Van Leeuwenhoek	10.1007/s10482-019-01299-z	2019	*
Enzymology	(R,R)-Butane-2,3-diol dehydrogenase from Bacillus clausii DSM 8716(T): Cloning and expression of the bdhA-gene, and initial characterization of enzyme.	Muschallik L, Molinnus D, Bongaerts J, Pohl M, Wagner T, Schoning MJ, Siegert P, Selmer T	J Biotechnol	10.1016/j.jbiotec.2017.07.020	2017	*
Phylogeny	Bacillus shacheensis sp. nov., a moderately halophilic bacterium isolated from a saline-alkali soil.	Lei Z, Qiu P, Ye R, Tian J, Liu Y, Wang L, Tang SK, Li WJ, Tian Y	J Gen Appl Microbiol	DN/JST.JSTAGE/jgam/60.101	2014	*
Phylogeny	Bacillus rhizosphaerae sp. nov., an novel diazotrophic bacterium isolated from sugarcane rhizosphere soil.	Madhaiyan M, Poonguzhali S, Lee JS, Lee KC, Hari K	Antonie Van Leeuwenhoek	10.1007/s10482-011-9600-3	2011	*
Biotechnology	Tolerance to challenges miming gastrointestinal transit by spores and vegetative cells of Bacillus clausii.	Cenci G, Trotta F, Caldini G	J Appl Microbiol	10.1111/j.1365-2672.2006.03042.x	2006	*
Genetics	Chromosomal aadD2 encodes an aminoglycoside nucleotidyltransferase in Bacillus clausii.	Bozdogan B, Galopin S, Gerbaud G, Courvalin P, Leclercq R	Antimicrob Agents Chemother	10.1128/AAC.47.4.1343-1346.2003	2003	*
Pathogenicity	Characterization of a new erm-related macrolide resistance gene present in probiotic strains of Bacillus clausii.	Bozdogan B, Galopin S, Leclercq R	Appl Environ Microbiol	10.1128/AEM.70.1.280-284.2004	2004	*
	Effect of Physical and Chemical Treatments on Viability, Sub-Lethal Injury, and Release of Cellular Components from Bacillus clausii and Bacillus coagulans Spores and Cells.	Bevilacqua A, Petruzzi L, Sinigaglia M, Speranza B, Campaniello D, Ciuffreda E, Corbo MR	Foods	10.3390/foods9121814	2020	*
	Probiotic characterization of Bacillus subtilis P223 isolated from kimchi.	Jeon HL, Lee NK, Yang SJ, Kim WS, Paik HD	Food Sci Biotechnol	10.1007/s10068-017-0148-5	2017	*

References

#3246

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 8716

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#35723 ; Curators of the CIP;
#57609 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 47262

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68371

Automatically annotated from API 50CH acid .

#68382

Automatically annotated from API zym .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#70798

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID14017.1 )

#118307 Collection of Institut Pasteur ; Curators of the CIP; CIP 104718

#124043

Dr. Isabel Schober, Dr. Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .

* These data were automatically processed and therefore are not curated

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Bacillus clausii strain DSM 8716

Alkalihalobacillus clausii DSM 8716

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Change proposal