Name: Spinactinospora alkalitolerans CXB 654
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 11265

Type strain:

Species: Spinactinospora alkalitolerans

Strain Designation: CXB 654

Culture col. no.: DSM 45414, LMG 25485, JCM 16559

Strain history: X.-H. Zhang CXB654.

NCBI tax ID(s): 687207 (species)

SI-ID 402650

DSM 45414

Section

Spinactinospora alkalitolerans CXB 654 is an aerobe, spore-forming, mesophilic bacterium that was isolated from marine sediment at a depth of 17.5 m.

Gram-positive
spore-forming
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Streptosporangiales*
	Family	*Nocardiopsidaceae*
	Genus	*Spinactinospora*
	Species	**Spinactinospora alkalitolerans**
	Full Scientific Name (LPSN)	Spinactinospora alkalitolerans Chang et al. 2011

Information on morphological and physiological properties Morphology

[Ref.: #29953]	Gram stain	positive

[Ref.: #29953]

Pigment production

Information on culture and growth conditions Culture and growth conditions

[Ref.: #16549]

Culture medium

BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)

[Ref.: #16549]

Culture medium growth

[Ref.: #16549]

Culture medium link

Medium recipe at MediaDive

[Ref.: #16549]

Culture medium composition

expand / minimize

[Ref.: #16549]

Culture medium

GYM STREPTOMYCES MEDIUM 10% NACL (DSMZ Medium 1159)

[Ref.: #16549]

Culture medium growth

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Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #16549]	growth	37 °C
[Ref.: #29953]	growth	16-44 °C
[Ref.: #29953]	optimum	37 °C
[Ref.: #67770]	growth	28 °C

[Ref.: #16549]	Temperature range	mesophilic
[Ref.: #29953]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #29953]			optimum	7.5
[Ref.: #29953]			growth	6.0-10.5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #29953]

Oxygen tolerance

aerobe

[Ref.: #29953]

Ability of spore formation

yes

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #29953]		NaCl		growth	01-15	%
[Ref.: #29953]		NaCl		optimum	5.5	%

[Ref.: #67770]

Observation

quinones: MK-10(H₈), MK-10(H₆), MK-9(H₈)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #29953]	2,3-butanediol ChEBI	+	carbon source
[Ref.: #29953]	alanine ChEBI	+	carbon source
[Ref.: #29953]	asparagine ChEBI	+	carbon source
[Ref.: #29953]	cellobiose ChEBI	+	carbon source
[Ref.: #29953]	dextrin ChEBI	+	carbon source
[Ref.: #29953]	fructose ChEBI	+	carbon source
[Ref.: #29953]	galactose ChEBI	+	carbon source
[Ref.: #29953]	gluconate ChEBI	+	carbon source
[Ref.: #29953]	glucose ChEBI	+	carbon source
[Ref.: #29953]	glutamate ChEBI	+	carbon source
[Ref.: #29953]	glycerol ChEBI	+	carbon source
[Ref.: #29953]	glycogen ChEBI	+	carbon source
[Ref.: #29953]	inosine ChEBI	+	carbon source
[Ref.: #29953]	L-alaninamide ChEBI	+	carbon source
[Ref.: #29953]	L-arabitol ChEBI	+	carbon source
[Ref.: #29953]	maltose ChEBI	+	carbon source
[Ref.: #29953]	mannose ChEBI	+	carbon source
[Ref.: #29953]	methyl D-glucoside ChEBI	+	carbon source
[Ref.: #29953]	N-acetylglucosamine ChEBI	+	carbon source
[Ref.: #29953]	nitrate ChEBI	+	reduction
[Ref.: #29953]	ribose ChEBI	+	carbon source
[Ref.: #29953]	serine ChEBI	+	carbon source
[Ref.: #29953]	tween 20 ChEBI	+	carbon source
[Ref.: #29953]	tween 40 ChEBI	+	carbon source
[Ref.: #29953]	tween 60 ChEBI	+	carbon source
[Ref.: #29953]	tween 80 ChEBI	+	carbon source
[Ref.: #29953]	xylitol ChEBI	+	carbon source
[Ref.: #29953]	xylose ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #29953]	catalase	+	1.11.1.6
[Ref.: #29953]	cytochrome oxidase	+	1.9.3.1
[Ref.: #29953]	urease	+	3.5.1.5

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #16549]	Sample type/isolated from	marine sediment at a depth of 17.5 m
[Ref.: #16549]	Geographic location (country and/or sea, region)	near the Yellow Sea Cold Water Mass
[Ref.: #16549]	Country	China
[Ref.: #16549]	Country ISO 3 Code	CHN
[Ref.: #16549]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Black soft mud at a depth of 17.5 m in the Yellow Sea
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Marine
#Environmental	#Aquatic	#Sediment

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

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Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #16549]	Spinactinospora alkalitolerans strain CXB654 16S ribosomal RNA gene, partial sequence	GU112453	1452	ENA	687207 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Spinactinospora alkalitolerans DSM 45414	2849348795	draft	JGI IMG/M	687207 tax ID	*

[Ref.: #16549]	GC-content	71.1 mol%	high performance liquid chromatography (HPLC)
[Ref.: #29953]	GC-content	71.1 mol%

Availability in culture collections External links

[Ref.: #16549]

Culture collection no.

DSM 45414, LMG 25485, JCM 16559

[Ref.: #80498]

SI-ID 402650

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Spinactinospora alkalitolerans gen. nov., sp. nov., an actinomycete isolated from marine sediment.	Chang X, Liu W, Zhang XH	Int J Syst Evol Microbiol	10.1099/ijs.0.027383-0	2011	*

References

#16549

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 45414

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#29953

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26322 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#80498

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID402650.1 )

#26322

IJSEM 2805 2011 ( DOI 10.1099/ijs.0.027383-0 , PubMed 21216917 )

* These data were automatically processed and therefore are not curated

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