Thermobifida fusca 190 Th, T-322, 190Th, A-0263 | Type strain | DSM 43792, ATCC 27730, CUB 1000, IFO 14071, JCM 3263, NBRC 14071, NCIB 11185, BCRC 12532, CECT 3320, CIP 105594, HAMBI 1548, HAMBI 483, IMSNU 22189, KCTC 9052, MTCC 1754, NCIMB 11185, NRRL B-8184, VKM Ac-1952, CCRC 12532

Caption:

Medium 83 45°C

Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

Culture medium

CZAPEK PEPTONE AGAR (DSMZ Medium 83)

Culture medium growth

Medium recipe at MediaDive

Culture medium link

Culture medium composition

Culture medium

ISP 2

Culture medium growth

Culture medium composition

Culture medium

ISP 5

Culture medium growth

Culture medium composition

Culture medium

ISP 7

Culture medium growth

Culture medium composition

[Ref.: #38567]

Culture medium

MEDIUM 3 - Columbia agar

[Ref.: #38567]

Culture medium growth

[Ref.: #38567]

Culture medium composition

Culture medium

GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)

Culture medium growth

Culture medium link

Culture medium

CYC-MEDIUM (modified following Cross and Attwell,1973) (DSMZ Medium 550)

Culture medium growth

Culture medium link

Culture medium

CIP Medium 3

Culture medium growth

Culture medium link

Culture medium

CIP Medium 9

Culture medium growth

Culture medium link

Culture medium

CIP Medium 57

Culture medium growth

Culture medium link

Culture medium

CIP Medium 236

Culture medium growth

Culture medium link

	Kind of temperature		Temperature
[Ref.: #11194]		growth	45 °C
[Ref.: #19588]		maximum	55 °C
[Ref.: #19588]		optimum	50 °C
[Ref.: #19588]		minimum	45 °C
[Ref.: #22955]		maximum	60 °C
[Ref.: #22955]		minimum	35 °C
[Ref.: #22957]		maximum	55 °C
[Ref.: #22957]		minimum	28 °C
[Ref.: #38567]		growth	45 °C
[Ref.: #67770]		growth	45 °C
[Ref.: #119499]		growth	37-45 °C
[Ref.: #119499]	no	growth	10 °C
[Ref.: #119499]	no	growth	25 °C
[Ref.: #119499]	no	growth	30 °C

[Ref.: #11194]	Temperature range	thermophilic
[Ref.: #19588]	Temperature range	thermophilic
[Ref.: #22955]	Temperature range	thermophilic
[Ref.: #22955]	Temperature range	mesophilic
[Ref.: #22957]	Temperature range	thermophilic
[Ref.: #22957]	Temperature range	mesophilic
[Ref.: #38567]	Temperature range	thermophilic
[Ref.: #67770]	Temperature range	thermophilic
[Ref.: #119499]	Temperature range	psychrophilic
[Ref.: #119499]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

Oxygen tolerance

obligate aerobe

[Ref.: #19588]	Spore description	Formation of spores, spore surface smooth
[Ref.: #19588]	Type of spore	spore
[Ref.: #19588]	Ability of spore formation	yes
[Ref.: #22955]	Spore description	Single spores, dichotomously branched sporophores
[Ref.: #22955]	Type of spore	spore
[Ref.: #22955]	Ability of spore formation	yes

What are isolation sources categories?

Name of produced compound

cellulase (=endo-1,4-ß-glucanase)

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #19588]		NaCl		maximum	2.5	%
[Ref.: #22957]		NaCl		growth	5	%(w/v)

[Ref.: #67770]

Observation

quinones: MK-10(H₆), MK-10(H₈), MK-11(H₆)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68371]	amygdalin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	arbutin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	cellobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #119499]	citrate ChEBI	-	carbon source
[Ref.: #22957]	D-arabinose ChEBI	-	growth
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #22957]	D-fructose ChEBI	+	growth
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-glucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #22957]	D-mannose ChEBI	+	growth
[Ref.: #68371]	D-mannose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #22956]	D-ribose ChEBI	-	growth
[Ref.: #22957]	D-ribose ChEBI	-	growth
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #119499]	esculin ChEBI	-	hydrolysis
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gentiobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #22956]	glucose ChEBI	+	builds acid from
[Ref.: #22956]	glycerol ChEBI	+	growth
[Ref.: #22957]	glycerol ChEBI	+	growth
[Ref.: #68371]	glycerol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycogen ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #119499]	hippurate ChEBI	+	hydrolysis
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #22956]	L-arabinose ChEBI	-	growth
[Ref.: #68371]	L-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #22956]	L-rhamnose ChEBI	-	growth
[Ref.: #22957]	L-rhamnose ChEBI	-	growth
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #22957]	L-sorbose ChEBI	-	growth
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #22957]	lactose ChEBI	+	growth
[Ref.: #68371]	lactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #22957]	maltose ChEBI	+	growth
[Ref.: #68371]	maltose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melezitose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #22956]	melibiose ChEBI	-	growth
[Ref.: #68371]	melibiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	N-acetylglucosamine ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #22957]	nitrate ChEBI	-	reduction
[Ref.: #119499]	nitrate ChEBI	-	reduction
[Ref.: #119499]	nitrite ChEBI	-	reduction
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #22956]	raffinose ChEBI	+	growth
[Ref.: #68371]	raffinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #22956]	ribitol ChEBI	+	growth
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	salicin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	starch ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #22957]	trehalose ChEBI	+	growth
[Ref.: #68371]	trehalose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #22957]	xylitol ChEBI	-	growth
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

	Antibiotica	Metabolite	Antibiotic sensitivity	Concentration
[Ref.: #22956]		erythromycin ChEBI	yes	20	µg/mL
[Ref.: #22956]		tetracycline ChEBI	yes	2.5	µg/mL

	Metabolite production	Metabolite	Production
[Ref.: #119499]		indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test	Methylred-test
[Ref.: #119499]		acetoin ChEBI	-
[Ref.: #119499]		glucose ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #119499]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #119499]	amylase	+
[Ref.: #119499]	beta-galactosidase	+	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #119499]	caseinase	+	3.4.21.50
[Ref.: #119499]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #119499]	DNase	-
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #119499]	gamma-glutamyltransferase	-	2.3.2.2
[Ref.: #119499]	gelatinase	+
[Ref.: #119499]	lecithinase	-
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #119499]	lipase	-
[Ref.: #68382]	lipase (C 14)	+		* from API zym
[Ref.: #119499]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #119499]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #119499]	oxidase	-
[Ref.: #119499]	phenylalanine ammonia-lyase	-	4.3.1.24
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #119499]	tryptophan deaminase	-
[Ref.: #119499]	tween esterase	+
[Ref.: #119499]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #119499]	10135	not determinedn.d.	-	-	-	-	+/-	-	-	-	-	+/-	-	+/-	-	+/-	-	-	-	-	-	-	-	-	-	-	+/-	-	-	-	-	-	+/-	-	-	-	-	-	-	-	-	+/-	+/-	+/-	-	-	-	-	-	-	+/-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #119499]	8825	-	-	+	+	+	+	-	-	-	-	-	+	-	-	-	+	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #11194]	Sample type/isolated from	soil

[Ref.: #119499]	Sample type/isolated from	Environment, Soil
[Ref.: #119499]	Geographic location (country and/or sea, region)	Leicester
[Ref.: #119499]	Country	United Kingdom
[Ref.: #119499]	Country ISO 3 Code	GBR
[Ref.: #119499]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Environmental

#Terrestrial

#Soil

[Ref.: #69479]

Global distribution of 16S sequence AF028245 (>99% sequence identity) for Thermobifida fusca subclade from Microbeatlas

Aquatic Samples	1658
Soil Samples	8508
Animal Samples	4336
Plant Samples	2067
Total Samples	16569

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #11194]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #19588]	Biosafety level	1	Risk group (German classification)
[Ref.: #119499]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #11194]	Thermomonospora fusca 16S ribosomal RNA gene, partial sequence	AF002264	1471	ENA	2021 tax ID
[Ref.: #11194]	Thermomonospora fusca 16S ribosomal RNA gene, partial sequence	AF028245	1480	ENA	2021 tax ID
[Ref.: #20218]	Thermobifida fusca gene for 16S ribosomal RNA, partial sequence	AB006171	1488	ENA	2021 tax ID	*

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Thermobifida fusca NBRC 14071 = JCM 3263	GCA_001592045	contig	GenBank	1223522 tax ID
[Ref.: #66792]	Thermobifida fusca NBRC 14071 = JCM 3263	GCA_001313945	contig	GenBank	1223522 tax ID	*
[Ref.: #66792]	Thermobifida fusca NBRC 14071 = JCM 3263	1223522.5	wgs	PATRIC	1223522 tax ID	*
[Ref.: #66792]	Thermobifida fusca NBRC 14071 = JCM 3263	1223522.4	wgs	PATRIC	1223522 tax ID	*
[Ref.: #66792]	Thermobifida fusca NBRC 14071, JCM 3263	2681813022	draft	JGI IMG/M	1223522 tax ID	*
[Ref.: #66792]	Thermobifida fusca NBRC 14071	2731957678	draft	JGI IMG/M	1223522 tax ID	*

[Ref.: #22957]	GC-content	66.2 mol%	high performance liquid chromatography (HPLC)
[Ref.: #67770]	GC-content	66.5 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #67770]	GC-content	67.5 mol%	genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Thermobifida fusca NBRC 14071 = JCM 3263 PATRIC: 1223522.5
[Ref.: #69480]	motile	no	93.521	no
[Ref.: #69480]	gram-positive	yes	91.734	yes
[Ref.: #69480]	anaerobic	no	98.485	no
[Ref.: #69480]	aerobic	yes	84.78	no
[Ref.: #69480]	halophile	no	56.156	no
[Ref.: #69480]	spore-forming	yes	87.036	yes
[Ref.: #69480]	thermophile	no	72.524	no
[Ref.: #69480]	glucose-util	yes	85.45	no
[Ref.: #69480]	flagellated	no	97.906	no
[Ref.: #69480]	glucose-ferment	no	91.374	no

Availability in culture collections External links