Nocardiopsis kunsanensis | Type strain | DSM 45168, KCTC 19473, JSM 073097, JCM 16954

Caption:

Medium 514 28°C

Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

Culture medium

BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)

Culture medium growth

Medium recipe at MediaDive

Culture medium link

Culture medium composition

Culture medium

ISP 2

Culture medium growth

Culture medium composition

Culture medium

ISP 3

Culture medium growth

Culture medium composition

Culture medium

ISP 4

Culture medium growth

Culture medium composition

Culture medium

ISP 5

Culture medium growth

Culture medium composition

Culture medium

ISP 6

Culture medium growth

Culture medium composition

Culture medium

ISP 7

Culture medium growth

Culture medium composition

What are isolation sources categories?

		Temperature
[Ref.: #15477]	growth	28 °C
[Ref.: #20172]	optimum	28 °C
[Ref.: #29257]	growth	20-35 °C
[Ref.: #29257]	optimum	25 °C
[Ref.: #67770]	growth	25 °C
[Ref.: #67771]	growth	28 °C

[Ref.: #15477]	Temperature range	mesophilic
[Ref.: #20172]	Temperature range	mesophilic
[Ref.: #29257]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #67771]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #29257]			optimum	8.5
[Ref.: #29257]			growth	6-10.5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #29257]	Oxygen tolerance	aerobe
[Ref.: #67771]	Oxygen tolerance	aerobe

[Ref.: #29257]	Ability of spore formation	yes
[Ref.: #69480]	Ability of spore formation	yes Confidence in %99.476

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #29257]		NaCl		growth	01-15	%
[Ref.: #29257]		NaCl		optimum	6	%

[Ref.: #29257]	Observation	aggregates in chains
[Ref.: #67770]	Observation	quinones: MK-10(H₄), MK-10(H₆), MK-10(H₈)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #29257]	alanine ChEBI	+	carbon source
[Ref.: #20172]	arabinose ChEBI	+
[Ref.: #20172]	cellulose ChEBI	+/-
[Ref.: #20172]	fructose ChEBI	+
[Ref.: #20172]	glucose ChEBI	+
[Ref.: #29257]	glucose ChEBI	+	carbon source
[Ref.: #20172]	mannitol ChEBI	+/-
[Ref.: #20172]	myo-inositol ChEBI	+/-
[Ref.: #20172]	raffinose ChEBI	+
[Ref.: #20172]	rhamnose ChEBI	+
[Ref.: #20172]	sucrose ChEBI	+
[Ref.: #29257]	sucrose ChEBI	+	carbon source
[Ref.: #20172]	xylose ChEBI	+
[Ref.: #29257]	xylose ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	+	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #29257]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #20172]	953	not determinedn.d.	+	+	+	-	+	+	-	-	+	+	+	-	-	-	+	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #15477]	Sample type/isolated from	sea anemone collected from a tidal flat
[Ref.: #15477]	Geographic location (country and/or sea, region)	South China Sea, Naozhou Island, near Zhanjiang City
[Ref.: #15477]	Country	China
[Ref.: #15477]	Country ISO 3 Code	CHN
[Ref.: #15477]	Continent	Asia

[Ref.: #67771]	Sample type/isolated from	From sea water
[Ref.: #67771]	Geographic location (country and/or sea, region)	Guangdong Province
[Ref.: #67771]	Country	China
[Ref.: #67771]	Country ISO 3 Code	CHN
[Ref.: #67771]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Homogenates of a sea anemone from a tidal flat on Naozhou Island in the South China Sea
[Ref.: #67770]	Geographic location (country and/or sea, region)	near Zhanjiang City, southern China
[Ref.: #67770]	Country	China
[Ref.: #67770]	Country ISO 3 Code	CHN
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Marine
#Environmental	#Terrestrial	#Tidal flat
#Host	#Invertebrates (Other)	#Cnidaria (Corals)

[Ref.: #69479]

Global distribution of 16S sequence EU583726 (>99% sequence identity) for Nocardiopsis from Microbeatlas

Aquatic Samples	28
Soil Samples	82
Animal Samples	376
Plant Samples	11
Total Samples	497

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #15477]	Nocardiopsis litoralis strain JSM 073097 16S ribosomal RNA gene, partial sequence	EU583726	1404	ENA	516701 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Nocardiopsis kunsanensis KCTC 19473	GCA_014651735	scaffold	GenBank	141693 tax ID	*
[Ref.: #66792]	Nocardiopsis litoralis strain KCTC 19473	516701.3	wgs	PATRIC	516701 tax ID	*

[Ref.: #15477]	GC-content	70.4 mol%	high performance liquid chromatography (HPLC)
[Ref.: #29257]	GC-content	70.4 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Nocardiopsis litoralis KCTC 19473 NCBI: GCA_014651735.1
[Ref.: #69481]	spore-forming	yes	78	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Nocardiopsis litoralis KCTC 19473 NCBI: GCA_014651735.1
[Ref.: #69480]	flagellated	no	97.995	yes
[Ref.: #69480]	gram-positive	yes	91.876	yes
[Ref.: #69480]	anaerobic	no	99.492	yes
[Ref.: #69480]	aerobic	yes	93.73	yes
[Ref.: #69480]	halophile	yes	82.609	yes
[Ref.: #69480]	spore-forming	yes	91.558	no
[Ref.: #69480]	glucose-util	yes	91.136	no
[Ref.: #69480]	thermophile	no	96.333	no
[Ref.: #69480]	motile	no	93.264	yes
[Ref.: #69480]	glucose-ferment	no	91.275	no

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