Name: Actinopolymorpha pittospori DSM 45354
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 11162

Type strain:

Species: Actinopolymorpha pittospori

Culture col. no.: DSM 45354, ACM 5288, JCM 18294, NRRL B-24810, PIP 143

Strain history: DSM 45354 <-- C. Franco PIP 143 <-- O. Kaewkla.

NCBI tax ID(s): 648752 (species)

SI-ID 403178

DSM 45354

Section

Actinopolymorpha pittospori DSM 45354 is a spore-forming, mesophilic bacterium that was isolated from surface-sterilized leave of the Australian native apricot tree Pittosporum phylliraeoides.

spore-forming
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Propionibacteriales*
	Family	*Actinopolymorphaceae*
	Genus	*Actinopolymorpha*
	Species	**Actinopolymorpha pittospori**
	Full Scientific Name (LPSN)	Actinopolymorpha pittospori Kaewkla and Franco 2011

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #69480]	Motility	no Confidence in %90.033

[Ref.: #69385]	Colony color	Beige (1001)
[Ref.: #69385]	Cultivation medium used	ISP 7

[Ref.: #69385]	Colony color	Ivory (1014)
[Ref.: #69385]	Cultivation medium used	ISP 5

[Ref.: #69385]	Colony color	Light ivory (1015)
[Ref.: #69385]	Cultivation medium used	ISP 2

[Ref.: #69385]	Colony color	Light ivory (1015)
[Ref.: #69385]	Cultivation medium used	ISP 6

[Ref.: #69385]	Colony color	Olive grey (7002)
[Ref.: #69385]	Cultivation medium used	suter with tyrosine

[Ref.: #69385]	Colony color	Olive yellow (1020)
[Ref.: #69385]	Cultivation medium used	suter without tyrosine

[Ref.: #69385]	Colony color	Oyster white (1013)
[Ref.: #69385]	Cultivation medium used	ISP 3

[Ref.: #69385]	Colony color	Oyster white (1013)
[Ref.: #69385]	Cultivation medium used	ISP 4

[Ref.: #69385]	Pigment production	yes
[Ref.: #69385]	Pigment name	Melanin
[Ref.: #69385]	Pigment production	yes
[Ref.: #69385]	Pigmentation color	Ocher brown (8001), beige (1001)
[Ref.: #69385]	Pigment name	soluble pigment

[Ref.: #69385]	Multicellular complex forming ability	no
[Ref.: #69385]	Multicellular complex name	Aerial mycelium
[Ref.: #69385]	Medium Name (multicellularity)	ISP 2

[Ref.: #69385]	Multicellular complex forming ability	no
[Ref.: #69385]	Multicellular complex name	Aerial mycelium
[Ref.: #69385]	Medium Name (multicellularity)	ISP 3

[Ref.: #69385]	Multicellular complex forming ability	no
[Ref.: #69385]	Multicellular complex name	Aerial mycelium
[Ref.: #69385]	Medium Name (multicellularity)	ISP 4

[Ref.: #69385]	Multicellular complex forming ability	no
[Ref.: #69385]	Multicellular complex name	Aerial mycelium
[Ref.: #69385]	Medium Name (multicellularity)	ISP 5

[Ref.: #69385]	Multicellular complex forming ability	no
[Ref.: #69385]	Multicellular complex name	Aerial mycelium
[Ref.: #69385]	Medium Name (multicellularity)	ISP 6

[Ref.: #69385]	Multicellular complex forming ability	no
[Ref.: #69385]	Multicellular complex name	Aerial mycelium
[Ref.: #69385]	Medium Name (multicellularity)	ISP 7

[Ref.: #69385]	Multicellular complex forming ability	no
[Ref.: #69385]	Multicellular complex name	Aerial mycelium
[Ref.: #69385]	Medium Name (multicellularity)	suter with tyrosine

[Ref.: #69385]	Multicellular complex forming ability	no
[Ref.: #69385]	Multicellular complex name	Aerial mycelium
[Ref.: #69385]	Medium Name (multicellularity)	suter without tyrosine

Multimedia content

[Ref.: #16346]

Caption:

Medium 65 28°C

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Intellectual property rights:

[Ref.: #69385]

Caption:

Plates (65, ISP2, ISP3, ISP4, ISP5, ISP7)

[Ref.: #69385]

Intellectual property rights:

Helmholtz-Zentrum für Infektionsforschung GmbH

[Ref.: #69385]

Caption:

Plates (65, ISP2, ISP3, ISP4, ISP5, ISP7)

[Ref.: #69385]

Intellectual property rights:

Helmholtz-Zentrum für Infektionsforschung GmbH

Information on culture and growth conditions Culture and growth conditions

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Culture medium

GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

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Culture medium

GPHF-MEDIUM (DSMZ Medium 553)

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Culture medium growth

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Culture medium link

Medium recipe provided by DSMZ

	Temperatures	Kind of temperature		Temperature
[Ref.: #16346]			growth	28 °C
[Ref.: #67770]			growth	28 °C

[Ref.: #16346]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #69481]	Ability of spore formation	yes Confidence in %90
[Ref.: #69480]	Ability of spore formation	yes Confidence in %96.578

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #69385]		NaCl		growth	0-2.5	%

[Ref.: #67770]

Observation

quinones: MK-9(H₄), MK-9(H₆), MK-10(H₄), MK-10(H₈)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #69385]	arabinose ChEBI	+	growth
[Ref.: #69385]	cellulose ChEBI	+/-	growth
[Ref.: #68379]	D-glucose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-mannitol ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-ribose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-xylose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	esculin ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #69385]	fructose ChEBI	+	growth
[Ref.: #68379]	gelatin ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #69385]	glucose ChEBI	+	growth
[Ref.: #68379]	glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #69385]	inositol ChEBI	+	growth
[Ref.: #68379]	lactose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	maltose ChEBI	-	fermentation	* from API Coryne
[Ref.: #69385]	mannose ChEBI	+/-	growth
[Ref.: #68379]	nitrate ChEBI	-	reduction	* from API Coryne
[Ref.: #69385]	raffinose ChEBI	+	growth
[Ref.: #69385]	rhamnose ChEBI	+	growth
[Ref.: #69385]	sucrose ChEBI	+	growth
[Ref.: #68379]	sucrose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	urea ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #69385]	xylose ChEBI	+	growth
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #68379]	alkaline phosphatase	+	3.1.3.1	* from API Coryne, API zym
[Ref.: #68379]	alpha-glucosidase	+	3.2.1.20	* from API Coryne, API zym
[Ref.: #68382]	alpha-mannosidase	+	3.2.1.24	* from API zym
[Ref.: #68379]	beta-galactosidase	+	3.2.1.23	* from API Coryne, API zym
[Ref.: #68379]	beta-glucosidase	+	3.2.1.21	* from API Coryne, API zym
[Ref.: #68379]	beta-glucuronidase	-	3.2.1.31	* from API Coryne, API zym
[Ref.: #68379]	gelatinase	+		* from API Coryne
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68379]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API Coryne, API zym
[Ref.: #68379]	pyrazinamidase	+	3.5.1.B15	* from API Coryne
[Ref.: #68379]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API Coryne
[Ref.: #68379]	urease	-	3.5.1.5	* from API Coryne
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #69385]	3878	-	+	-	+	-	+	+	+	+	-	+	not determinedn.d.	-	-	-	-	-	-	-	-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #69385]	6062	not determinedn.d.	+	+/-	+/-	+/-	+	+	+/-	+/-	+/-	+/-	+/-	+/-	+	-	+	+	+	+	+/-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #16346]	Sample type/isolated from	surface-sterilized leave of the Australian native apricot tree Pittosporum phylliraeoides
[Ref.: #16346]	Host species	Pittosporum phylliraeoides
[Ref.: #16346]	Geographic location (country and/or sea, region)	Adelaide, Flinders University campus
[Ref.: #16346]	Country	Australia
[Ref.: #16346]	Country ISO 3 Code	AUS
[Ref.: #16346]	Continent	Australia and Oceania

[Ref.: #67770]	Sample type/isolated from	Leaves of Pittosporum phylliraeoides growing on the campus of Flinders Univ.
[Ref.: #67770]	Host species	Pittosporum phylliraeoides
[Ref.: #67770]	Geographic location (country and/or sea, region)	Adelaide, South Australia
[Ref.: #67770]	Country	Australia
[Ref.: #67770]	Country ISO 3 Code	AUS
[Ref.: #67770]	Continent	Australia and Oceania
* marker position based on {}

Isolation sources categories

#Host	#Plants	#Tree
#Host Body-Site	#Plant	#Leaf (Phyllosphere)
#Host Body-Site	#Plant	#Sterilized plant part

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence FJ805429 (>99% sequence identity) for Actinopolymorpha pittospori from Microbeatlas

Aquatic Samples	37
Soil Samples	786
Animal Samples	47
Plant Samples	63
Total Samples	933

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

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Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #16346]	Actinopolymorpha pittospori strain PIP 143 16S ribosomal RNA gene, partial sequence	FJ805429	1407	ENA	648752 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Actinopolymorpha pittospori DSM 45354	GCA_014873875	contig	GenBank	648752 tax ID	*
[Ref.: #66792]	Actinopolymorpha pittospori strain DSM 45354	648752.3	wgs	PATRIC	648752 tax ID	*
[Ref.: #66792]	Actinopolymorpha pittospori DSM 45354	2880531579	draft	JGI IMG/M	648752 tax ID	*

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GC-content

69.6 mol%

high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Actinopolymorpha pittospori strain DSM 45354 PATRIC: 648752.3
[Ref.: #69481]	spore-forming	yes	90	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Actinopolymorpha pittospori strain DSM 45354 PATRIC: 648752.3
[Ref.: #69480]	gram-positive	yes	89.659	no
[Ref.: #69480]	anaerobic	no	98.861	no
[Ref.: #69480]	halophile	no	91.541	no
[Ref.: #69480]	spore-forming	yes	78.473	no
[Ref.: #69480]	glucose-util	yes	85.772	yes
[Ref.: #69480]	thermophile	no	97.442	yes
[Ref.: #69480]	flagellated	no	98.534	no
[Ref.: #69480]	aerobic	yes	93.697	no
[Ref.: #69480]	motile	no	90.712	no
[Ref.: #69480]	glucose-ferment	no	88.013	no

Availability in culture collections External links

[Ref.: #16346]

Culture collection no.

DSM 45354, ACM 5288, JCM 18294, NRRL B-24810, PIP 143

[Ref.: #80411]

SI-ID 403178

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Tenggerimyces mesophilus gen. nov., sp. nov., a member of the family Nocardioidaceae.	Sun HM, Zhang T, Wei YZ, Liu HY, Yu LY, Zhang YQ	Int J Syst Evol Microbiol	10.1099/ijsem.0.000421	2015	*
Phylogeny	Actinopolymorpha pittospori sp. nov., an endophyte isolated from surface-sterilized leaves of an apricot tree (Pittosporum phylliraeoides).	Kaewkla O, Franco CMM	Int J Syst Evol Microbiol	10.1099/ijs.0.029579-0	2010	*

References

#16346

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 45354

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68379

Automatically annotated from API Coryne .

#68382

Automatically annotated from API zym .

#69385

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#80411

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID403178.1 )

* These data were automatically processed and therefore are not curated

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Actinopolymorpha pittospori strain DSM 45354

Actinopolymorpha pittospori strain DSM 45354

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