Name: Nocardioides daejeonensis MJ31
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 11155

Type strain:

Species: Nocardioides daejeonensis

Strain Designation: MJ31

Culture col. no.: DSM 25320, JCM 16922, KCTC 19772

Strain history: M. K. Kim MJ31.

NCBI tax ID(s): 1046556 (species)

SI-ID 398561

DSM 25320

Section

Nocardioides daejeonensis MJ31 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from sludge sample of a sewage disposal plant.

Gram-positive
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Propionibacteriales*
	Family	*Nocardioidaceae*
	Genus	*Nocardioides*
	Species	**Nocardioides daejeonensis**
	Full Scientific Name (LPSN)	Nocardioides daejeonensis Woo et al. 2012

Information on morphological and physiological properties Morphology

[Ref.: #30315]	Gram stain	positive

[Ref.: #30315]	Cell length	0.8-2 µm

[Ref.: #30315]	Cell width	0.5-0.8 µm

[Ref.: #30315]	Cell shape	rod-shaped

[Ref.: #30315]	Motility	no

[Ref.: #69321]	Colony color	Beige (1001)
[Ref.: #69321]	Cultivation medium used	ISP 2

[Ref.: #69321]	Colony color	Beige (1001)
[Ref.: #69321]	Cultivation medium used	ISP 6

[Ref.: #69321]	Colony color	Beige (1001)
[Ref.: #69321]	Cultivation medium used	suter with tyrosine

[Ref.: #69321]	Colony color	Ivory (1014)
[Ref.: #69321]	Cultivation medium used	suter without tyrosine

[Ref.: #69321]	Colony color	Light ivory (1015)
[Ref.: #69321]	Cultivation medium used	ISP 7

[Ref.: #69321]	Colony color	Oyster white (1013)
[Ref.: #69321]	Cultivation medium used	ISP 3

[Ref.: #69321]	Colony color	Oyster white (1013)
[Ref.: #69321]	Cultivation medium used	ISP 4

[Ref.: #69321]	Colony color	Oyster white (1013), ivory (1014)
[Ref.: #69321]	Cultivation medium used	ISP 5

[Ref.: #30315]	Pigment production	yes
[Ref.: #69321]	Pigment production	no
[Ref.: #69321]	Pigment name	Melanin
[Ref.: #69321]	Pigment production	no
[Ref.: #69321]	Pigment name	soluble pigment

[Ref.: #69321]	Multicellular complex forming ability	no
[Ref.: #69321]	Multicellular complex name	Aerial mycelium
[Ref.: #69321]	Medium Name (multicellularity)	ISP 2

[Ref.: #69321]	Multicellular complex forming ability	no
[Ref.: #69321]	Multicellular complex name	Aerial mycelium
[Ref.: #69321]	Medium Name (multicellularity)	ISP 3

[Ref.: #69321]	Multicellular complex forming ability	no
[Ref.: #69321]	Multicellular complex name	Aerial mycelium
[Ref.: #69321]	Medium Name (multicellularity)	ISP 4

[Ref.: #69321]	Multicellular complex forming ability	no
[Ref.: #69321]	Multicellular complex name	Aerial mycelium
[Ref.: #69321]	Medium Name (multicellularity)	ISP 5

[Ref.: #69321]	Multicellular complex forming ability	no
[Ref.: #69321]	Multicellular complex name	Aerial mycelium
[Ref.: #69321]	Medium Name (multicellularity)	ISP 6

[Ref.: #69321]	Multicellular complex forming ability	no
[Ref.: #69321]	Multicellular complex name	Aerial mycelium
[Ref.: #69321]	Medium Name (multicellularity)	ISP 7

[Ref.: #69321]	Multicellular complex forming ability	no
[Ref.: #69321]	Multicellular complex name	Aerial mycelium
[Ref.: #69321]	Medium Name (multicellularity)	suter with tyrosine

[Ref.: #69321]	Multicellular complex forming ability	no
[Ref.: #69321]	Multicellular complex name	Aerial mycelium
[Ref.: #69321]	Medium Name (multicellularity)	suter without tyrosine

Multimedia content

[Ref.: #69321]

Caption:

(ISP6, ISP7)

[Ref.: #69321]

Intellectual property rights:

Helmholtz-Zentrum für Infektionsforschung GmbH

[Ref.: #69321]

Caption:

(ISP6, ISP7)

[Ref.: #69321]

Intellectual property rights:

Helmholtz-Zentrum für Infektionsforschung GmbH

Information on culture and growth conditions Culture and growth conditions

[Ref.: #17996]

Culture medium

R2A MEDIUM (DSMZ Medium 830)

[Ref.: #17996]

Culture medium growth

[Ref.: #17996]

Culture medium link

Medium recipe at MediaDive

[Ref.: #17996]

Culture medium composition

expand / minimize

[Ref.: #17996]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

[Ref.: #17996]

Culture medium growth

[Ref.: #17996]

Culture medium link

Medium recipe at MediaDive

[Ref.: #17996]

Culture medium composition

expand / minimize

[Ref.: #17996]

Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

[Ref.: #17996]

Culture medium growth

[Ref.: #17996]

Culture medium link

Medium recipe at MediaDive

[Ref.: #17996]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #17996]	growth	28 °C
[Ref.: #30315]	growth	15-37 °C
[Ref.: #30315]	optimum	30 °C
[Ref.: #67770]	growth	28 °C

[Ref.: #17996]	Temperature range	mesophilic
[Ref.: #30315]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #30315]			optimum	7.5-8
[Ref.: #30315]			growth	5.5-9

Information on physiology and metabolism Physiology and metabolism

[Ref.: #30315]

Oxygen tolerance

aerobe

	Salt		Salt conc.
[Ref.: #30315]	NaCl	growth	0-10	%
[Ref.: #30315]	NaCl	optimum	2	%
[Ref.: #69321]	NaCl	growth	0-5	%

[Ref.: #67770]

Observation

quinones: MK-8(H₄)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #30315]	2-dehydro-D-gluconate ChEBI	+	carbon source
[Ref.: #30315]	3-hydroxybenzoate ChEBI	+	carbon source
[Ref.: #30315]	4-hydroxybenzoate ChEBI	+	carbon source
[Ref.: #69321]	arabinose ChEBI	+	growth
[Ref.: #30315]	arabinose ChEBI	+	carbon source
[Ref.: #69321]	cellulose ChEBI	-	growth
[Ref.: #30315]	citrate ChEBI	+	carbon source
[Ref.: #68379]	D-glucose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-mannitol ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-ribose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-xylose ChEBI	-	fermentation	* from API Coryne
[Ref.: #30315]	decanoate ChEBI	+	carbon source
[Ref.: #30315]	esculin ChEBI	+	hydrolysis
[Ref.: #68379]	esculin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #69321]	fructose ChEBI	+	growth
[Ref.: #68379]	gelatin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #69321]	glucose ChEBI	+	growth
[Ref.: #68379]	glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #69321]	inositol ChEBI	+	growth
[Ref.: #30315]	lactate ChEBI	+	carbon source
[Ref.: #68379]	lactose ChEBI	-	fermentation	* from API Coryne
[Ref.: #30315]	malonate ChEBI	+	carbon source
[Ref.: #30315]	maltose ChEBI	+	carbon source
[Ref.: #68379]	maltose ChEBI	-	fermentation	* from API Coryne
[Ref.: #69321]	mannose ChEBI	+	growth
[Ref.: #30315]	nitrate ChEBI	+	reduction
[Ref.: #68379]	nitrate ChEBI	+	reduction	* from API Coryne
[Ref.: #69321]	raffinose ChEBI	+	growth
[Ref.: #30315]	rhamnose ChEBI	+	carbon source
[Ref.: #69321]	rhamnose ChEBI	+	growth
[Ref.: #30315]	serine ChEBI	+	carbon source
[Ref.: #69321]	sucrose ChEBI	+	growth
[Ref.: #68379]	sucrose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	urea ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #69321]	xylose ChEBI	+	growth
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #30315]	acid phosphatase	+	3.1.3.2
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #30315]	alkaline phosphatase	+	3.1.3.1
[Ref.: #68379]	alkaline phosphatase	+	3.1.3.1	* from API Coryne, API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68379]	alpha-glucosidase	-	3.2.1.20	* from API Coryne, API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68379]	beta-galactosidase	-	3.2.1.23	* from API Coryne
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68379]	beta-glucosidase	-	3.2.1.21	* from API Coryne
[Ref.: #68379]	beta-glucuronidase	-	3.2.1.31	* from API Coryne, API zym
[Ref.: #30315]	catalase	+	1.11.1.6
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68379]	gelatinase	-		* from API Coryne
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68379]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API Coryne, API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #68379]	pyrazinamidase	-	3.5.1.B15	* from API Coryne
[Ref.: #68379]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API Coryne
[Ref.: #68379]	urease	-	3.5.1.5	* from API Coryne
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #69321]	3814	+	-	-	+	-	-	-	-	-	-	-	not determinedn.d.	-	-	-	-	-	-	-	-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #69321]	5998	not determinedn.d.	+	+	+	+/-	+	+	+/-	+/-	-	+	+	-	+/-	-	-	+	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #17996]	Sample type/isolated from	sludge sample of a sewage disposal plant
[Ref.: #17996]	Geographic location (country and/or sea, region)	Daejeon
[Ref.: #17996]	Country	Republic of Korea
[Ref.: #17996]	Country ISO 3 Code	KOR
[Ref.: #17996]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Sludge at the Daejeon sewage-disposal plant
[Ref.: #67770]	Country	Republic of Korea
[Ref.: #67770]	Country ISO 3 Code	KOR
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Engineered	#Waste	#Water treatment plant
#Environmental	#Terrestrial	#Mud (Sludge)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence JF937066 (>99% sequence identity) for Nocardioides daejeonensis from Microbeatlas

Aquatic Samples	37
Soil Samples	53
Animal Samples	170
Plant Samples	14
Total Samples	274

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #17996]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #17996]	Nocardioides daejeonensis strain MJ31 16S ribosomal RNA gene, partial sequence	JF937066	1468	ENA	1046556 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Nocardioides daejeonensis MJ31	GCA_003194585	contig	GenBank	1046556 tax ID
[Ref.: #66792]	Nocardioides daejeonensis MJ31	2866264325	draft	JGI IMG/M	1046556 tax ID	*

[Ref.: #17996]	GC-content	71.2 mol%	high performance liquid chromatography (HPLC)
[Ref.: #30315]	GC-content	71.2 mol%

Availability in culture collections External links

[Ref.: #17996]

Culture collection no.

DSM 25320, JCM 16922, KCTC 19772

[Ref.: #80404]

SI-ID 398561

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Nocardioides houyundeii sp. nov., isolated from Tibetan antelope faeces.	Wang X, Yang J, Lu S, Lai XH, Jin D, Pu J, Zhang G, Huang Y, Zhu W, Wu X, Liang H, Xu J	Int J Syst Evol Microbiol	10.1099/ijsem.0.003076	2018	*
Phylogeny	Nocardioides gilvus sp. nov., isolated from Namtso Lake.	Zhang HX, Wang K, Xu ZX, Chen GJ, Du ZJ	Antonie Van Leeuwenhoek	10.1007/s10482-016-0735-0	2016	*
Phylogeny	Nocardioides pakistanensis sp. nov., isolated from a hot water spring of Tatta Pani in Pakistan.	Amin A, Ahmed I, Habib N, Abbas S, Xiao M, Hozzein WN, Li WJ	Antonie Van Leeuwenhoek	10.1007/s10482-016-0711-8	2016	*
Phylogeny	Nocardioides solisilvae sp. nov., isolated from a forest soil.	Sultanpuram VR, Mothe T, Mohammed F	Antonie Van Leeuwenhoek	10.1007/s10482-015-0455-x	2015	*
Phylogeny	Nocardioides echinoideorum sp. nov., isolated from sea urchins (Tripneustes gratilla).	Lin SY, Wen CZ, Hameed A, Liu YC, Hsu YH, Shen FT, Lai WA, Young CC	Int J Syst Evol Microbiol	10.1099/ijs.0.000206	2015	*
Phylogeny	Nocardioides daejeonensis sp. nov., a denitrifying bacterium isolated from sludge in a sewage-disposal plant.	Woo SG, Srinivasan S, Yang J, Jung YA, Kim MK, Lee M	Int J Syst Evol Microbiol	10.1099/ijs.0.033308-0	2011	*

References

#17996

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 25320

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#30315

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26656 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68379

Automatically annotated from API Coryne .

#68382

Automatically annotated from API zym .

#69321

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#80404

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID398561.1 )

#26656

IJSEM 1199 2012 ( DOI 10.1099/ijs.0.033308-0 , PubMed 21742819 )

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Nocardioides daejeonensis in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF