Name: Nocardioides hungaricus 1RaM5-12
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 11151

Type strain:

Species: Nocardioides hungaricus

Strain Designation: 1RaM5-12, RaM5-12

Culture col. no.: DSM 21673, JCM 19558, NCAIM 02330, NCAIM B.02330

Strain history: DSM 21673 <-- E. M. Tóth RaM5-12 (=1RaM5-12).

NCBI tax ID(s): 520089 (species)

SI-ID 396761

DSM 21673

Section

Nocardioides hungaricus 1RaM5-12 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from drinking water network.

Gram-positive
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Propionibacteriales*
	Family	*Nocardioidaceae*
	Genus	*Nocardioides*
	Species	**Nocardioides hungaricus**
	Full Scientific Name (LPSN)	Nocardioides hungaricus Tóth et al. 2011

Information on morphological and physiological properties Morphology

[Ref.: #29860]	Gram stain	positive

[Ref.: #29860]	Cell length	1.25 µm

[Ref.: #29860]	Cell width	0.5 µm

[Ref.: #29860]	Cell shape	rod-shaped

[Ref.: #29860]	Motility	no

[Ref.: #21355]	Colony color	Sand yellow (1002)
[Ref.: #21355]	Incubation period	10-14 days
[Ref.: #21355]	Cultivation medium used	ISP 2

[Ref.: #21355]	Colony color	Light ivory (1015)
[Ref.: #21355]	Incubation period	10-14 days
[Ref.: #21355]	Cultivation medium used	ISP 3

[Ref.: #21355]	Incubation period	10-14 days
[Ref.: #21355]	Cultivation medium used	ISP 4

[Ref.: #21355]	Incubation period	10-14 days
[Ref.: #21355]	Cultivation medium used	ISP 5

[Ref.: #21355]	Incubation period	10-14 days
[Ref.: #21355]	Cultivation medium used	ISP 7

[Ref.: #21355]	Incubation period	10-14 days
[Ref.: #21355]	Cultivation medium used	suter with tyrosine

[Ref.: #21355]	Incubation period	10-14 days
[Ref.: #21355]	Cultivation medium used	suter without tyrosine

[Ref.: #21355]	Multicellular complex forming ability	no
[Ref.: #21355]	Medium Name (multicellularity)	ISP 2

[Ref.: #21355]	Multicellular complex forming ability	no
[Ref.: #21355]	Medium Name (multicellularity)	ISP 3

[Ref.: #21355]	Multicellular complex forming ability	no
[Ref.: #21355]	Medium Name (multicellularity)	ISP 4

[Ref.: #21355]	Multicellular complex forming ability	no
[Ref.: #21355]	Medium Name (multicellularity)	ISP 5

[Ref.: #21355]	Multicellular complex forming ability	no
[Ref.: #21355]	Medium Name (multicellularity)	ISP 7

[Ref.: #21355]	Multicellular complex forming ability	no
[Ref.: #21355]	Medium Name (multicellularity)	Suter with tyrosine

[Ref.: #21355]	Multicellular complex forming ability	no
[Ref.: #21355]	Medium Name (multicellularity)	Suter without tyrosine

Information on culture and growth conditions Culture and growth conditions

[Ref.: #15878]

Culture medium

R2A MEDIUM (DSMZ Medium 830)

[Ref.: #15878]

Culture medium growth

[Ref.: #15878]

Culture medium link

Medium recipe at MediaDive

[Ref.: #15878]

Culture medium composition

expand / minimize

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Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

[Ref.: #15878]

Culture medium growth

[Ref.: #15878]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #21355]

Culture medium

ISP 2

[Ref.: #21355]

Culture medium growth

[Ref.: #21355]

Culture medium composition

expand / minimize

[Ref.: #21355]

Culture medium

ISP 3

[Ref.: #21355]

Culture medium growth

[Ref.: #21355]

Culture medium composition

expand / minimize

[Ref.: #21355]

Culture medium

ISP 4

[Ref.: #21355]

Culture medium growth

[Ref.: #21355]

Culture medium composition

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[Ref.: #21355]

Culture medium

ISP 5

[Ref.: #21355]

Culture medium growth

[Ref.: #21355]

Culture medium composition

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[Ref.: #21355]

Culture medium

ISP 7

[Ref.: #21355]

Culture medium growth

[Ref.: #21355]

Culture medium composition

expand / minimize

[Ref.: #21355]

Culture medium

suter without tyrosine

[Ref.: #21355]

Culture medium growth

[Ref.: #21355]

Culture medium composition

expand / minimize

[Ref.: #21355]

Culture medium

suter with tyrosine

[Ref.: #21355]

Culture medium growth

[Ref.: #21355]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #15878]	growth	28 °C
[Ref.: #29860]	growth	20-37 °C
[Ref.: #29860]	optimum	24 °C
[Ref.: #67770]	growth	28 °C

[Ref.: #15878]	Temperature range	mesophilic
[Ref.: #29860]	Temperature range	psychrophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #29860]			optimum	6.5
[Ref.: #29860]			growth	05-09

Information on physiology and metabolism Physiology and metabolism

[Ref.: #29860]

Oxygen tolerance

aerobe

[Ref.: #29860]

Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #21355]		NaCl		growth	0	%
[Ref.: #29860]		NaCl		growth	0-2.5	%

[Ref.: #15878]	Murein short key	A41.01
[Ref.: #15878]	Murein types	A3gamma LL-Dpm-Gly

[Ref.: #67770]

Observation

quinones: MK-8(H₄)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #21355]	arabinose ChEBI	-
[Ref.: #21355]	cellulose ChEBI	+
[Ref.: #68379]	D-glucose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-mannitol ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-ribose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-xylose ChEBI	-	fermentation	* from API Coryne
[Ref.: #29860]	esculin ChEBI	+	hydrolysis
[Ref.: #21355]	fructose ChEBI	-
[Ref.: #68379]	gelatin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #21355]	glucose ChEBI	-
[Ref.: #68379]	glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	lactose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	maltose ChEBI	-	fermentation	* from API Coryne
[Ref.: #21355]	mannose ChEBI	-
[Ref.: #21355]	myo-inositol ChEBI	-
[Ref.: #29860]	nitrate ChEBI	+	reduction
[Ref.: #68379]	nitrate ChEBI	-	reduction	* from API Coryne
[Ref.: #21355]	raffinose ChEBI	-
[Ref.: #21355]	rhamnose ChEBI	-
[Ref.: #21355]	sucrose ChEBI	-
[Ref.: #68379]	sucrose ChEBI	-	fermentation	* from API Coryne
[Ref.: #29860]	tween 80 ChEBI	+	carbon source
[Ref.: #68379]	urea ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #21355]	xylose ChEBI	-
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #29860]	acid phosphatase	+	3.1.3.2
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #29860]	alkaline phosphatase	+	3.1.3.1
[Ref.: #68379]	alkaline phosphatase	+	3.1.3.1	* from API Coryne, API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68379]	alpha-glucosidase	+	3.2.1.20	* from API Coryne, API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym
[Ref.: #68379]	beta-galactosidase	-	3.2.1.23	* from API Coryne
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68379]	beta-glucuronidase	-	3.2.1.31	* from API Coryne, API zym
[Ref.: #29860]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #29860]	gelatinase	+
[Ref.: #68379]	gelatinase	-		* from API Coryne
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68379]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API Coryne, API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #68379]	pyrazinamidase	-	3.5.1.B15	* from API Coryne
[Ref.: #68379]	pyrrolidonyl arylamidase	+	3.4.19.3	* from API Coryne
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68379]	urease	-	3.5.1.5	* from API Coryne
	Only first 10 entries are displayed. Click here to see all.

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #21355]	484	-	-	+	+	-	-	+	-	+/-	-	-	not determinedn.d.	-	-	-	-	-	-	-	-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #21355]	996	not determinedn.d.	+	+	+	+/-	+	+/-	-	-	-	+	+	-	+	-	+	+	-	-	+/-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #15878]	Sample type/isolated from	drinking water network
[Ref.: #15878]	Geographic location (country and/or sea, region)	Budapest
[Ref.: #15878]	Country	Hungary
[Ref.: #15878]	Country ISO 3 Code	HUN
[Ref.: #15878]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Drinking water supply system of Budapest
[Ref.: #67770]	Country	Hungary
[Ref.: #67770]	Country ISO 3 Code	HUN
[Ref.: #67770]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Environmental

#Aquatic

#Freshwater

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AM981198 (>99% sequence identity) for Nocardioides hungaricus subclade from Microbeatlas

Aquatic Samples	85
Soil Samples	560
Animal Samples	121
Plant Samples	110
Total Samples	876

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #15878]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #15878]	Nocardioides hungaricus partial 16S rRNA gene, type strain 1RaM5-12T	AM981198	1462	ENA	520089 tax ID

[Ref.: #15878]	GC-content	71.4 mol%	high performance liquid chromatography (HPLC)
[Ref.: #29860]	GC-content	71.4 mol%

Availability in culture collections External links

[Ref.: #15878]

Culture collection no.

DSM 21673, JCM 19558, NCAIM 02330, NCAIM B.02330

[Ref.: #80400]

SI-ID 396761

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Nocardioides hungaricus sp. nov., isolated from a drinking water supply system.	Toth EM, Keki Z, Makk J, Homonnay ZG, Marialigeti K, Schumann P	Int J Syst Evol Microbiol	10.1099/ijs.0.022939-0	2010	*

References

#15878

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 21673

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#21355

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#29860

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26234 (see below)

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68379

Automatically annotated from API Coryne .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#80400

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID396761.1 )

#26234

IJSEM 549 2011 ( DOI 10.1099/ijs.0.022939-0 , PubMed 20382790 )

* These data were automatically processed and therefore are not curated

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