Name: Nocardioides hankookensis DS-30
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 11133

Type strain:

Species: Nocardioides hankookensis

Strain Designation: DS-30

Culture col. no.: DSM 21479, CCUG 54522, KCTC 19246, JCM 15302

Strain history: <- JH Yoon, KRIBB

NCBI tax ID(s): 443157 (species)

SI-ID 323980

CCUG 54522, KCTC 19246, JCM 15302, DSM 21479

Section

Nocardioides hankookensis DS-30 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from soil.

Gram-positive
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Propionibacteriales*
	Family	*Nocardioidaceae*
	Genus	*Nocardioides*
	Species	**Nocardioides hankookensis**
	Full Scientific Name (LPSN)	Nocardioides hankookensis Yoon et al. 2008

Information on morphological and physiological properties Morphology

[Ref.: #32290]	Gram stain	positive
[Ref.: #67771]	Gram stain	positive

[Ref.: #32290]	Cell length	5.75 µm

[Ref.: #32290]	Cell width	0.6 µm

[Ref.: #32290]	Cell shape	rod-shaped

[Ref.: #32290]	Motility	no

[Ref.: #60212]

Incubation period

27 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #15730]

Culture medium

R2A MEDIUM (DSMZ Medium 830)

[Ref.: #15730]

Culture medium growth

[Ref.: #15730]

Culture medium link

Medium recipe at MediaDive

[Ref.: #15730]

Culture medium composition

expand / minimize

[Ref.: #15730]

Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

[Ref.: #15730]

Culture medium growth

[Ref.: #15730]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #15730]	growth	28 °C
[Ref.: #32290]	growth	10-34 °C
[Ref.: #60212]	growth	30 °C
[Ref.: #67770]	growth	28 °C
[Ref.: #67771]	growth	30 °C

[Ref.: #15730]	Temperature range	mesophilic
[Ref.: #60212]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #67771]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #32290]			optimum	6.5
[Ref.: #32290]			growth	5.5-8

Information on physiology and metabolism Physiology and metabolism

[Ref.: #32290]	Oxygen tolerance	aerobe
[Ref.: #60212]	Oxygen tolerance	aerobe
[Ref.: #67771]	Oxygen tolerance	aerobe

[Ref.: #32290]

Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #32290]		NaCl		growth	0-2	%
[Ref.: #32290]		NaCl		optimum	0.25	%

[Ref.: #67770]

Observation

quinones: MK-8(H₄)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #32290]	adipate ChEBI	+	carbon source
[Ref.: #32290]	arabinose ChEBI	+	carbon source
[Ref.: #32290]	galactose ChEBI	+	carbon source
[Ref.: #32290]	gluconate ChEBI	+	carbon source
[Ref.: #32290]	malate ChEBI	+	carbon source
[Ref.: #32290]	mannitol ChEBI	+	carbon source
[Ref.: #32290]	melibiose ChEBI	+	carbon source
[Ref.: #32290]	rhamnose ChEBI	+	carbon source

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #32290]		alkaline phosphatase	+	3.1.3.1
[Ref.: #32290]		cytochrome oxidase	+	1.9.3.1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #15730]	Sample type/isolated from	soil
[Ref.: #15730]	Geographic location (country and/or sea, region)	Dokdo
[Ref.: #15730]	Country	Republic of Korea
[Ref.: #15730]	Country ISO 3 Code	KOR
[Ref.: #15730]	Continent	Asia

[Ref.: #60212]	Sample type/isolated from	Soil
[Ref.: #60212]	Sampling date	2004-05-01
[Ref.: #60212]	Geographic location (country and/or sea, region)	Dokdo
[Ref.: #60212]	Country	Republic of Korea
[Ref.: #60212]	Country ISO 3 Code	KOR
[Ref.: #60212]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Soil

[Ref.: #67771]	Sample type/isolated from	From soil
[Ref.: #67771]	Geographic location (country and/or sea, region)	Dokdo
[Ref.: #67771]	Country	Republic of Korea
[Ref.: #67771]	Country ISO 3 Code	KOR
[Ref.: #67771]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental

#Terrestrial

#Soil

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence EF555584 (>99% sequence identity) for Nocardioides hankookensis from Microbeatlas

Aquatic Samples	164
Soil Samples	1533
Animal Samples	673
Plant Samples	55
Total Samples	2425

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #15730]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #15730]	Nocardioides hankookensis strain DS-30 16S ribosomal RNA gene, partial sequence	EF555584	1447	ENA	443157 tax ID

[Ref.: #15730]	GC-content	71.3 mol%
[Ref.: #67770]	GC-content	71.3 mol%	high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #15730]

Culture collection no.

DSM 21479, CCUG 54522, KCTC 19246, JCM 15302

[Ref.: #80382]

SI-ID 323980

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Nocardioides intraradicalis sp. nov., isolated from the roots of Psammosilene tunicoides W. C. Wu et C. Y. Wu.	Huang MJ, Huang HQ, Salam N, Xiao M, Duan YQ, Kim CJ, Li QQ, Chen W, Li WJ	Int J Syst Evol Microbiol	10.1099/ijsem.0.001274	2016	*
Phylogeny	Nocardioides soli sp. nov., a carbendazim-degrading bacterium isolated from soil under the long-term application of carbendazim.	Sun LN, Zhang J, Gong FF, Wang X, Hu G, Li SP, Hong Q	Int J Syst Evol Microbiol	10.1099/ijs.0.057935-0	2014	*
Phylogeny	Nocardioides endophyticus sp. nov. and Nocardioides conyzicola sp. nov., isolated from herbaceous plant roots.	Han JH, Kim TS, Joung Y, Kim MN, Shin KS, Bae T, Kim SB	Int J Syst Evol Microbiol	10.1099/ijs.0.054619-0	2013	*
Phylogeny	Nocardioides caricicola sp. nov., an endophytic bacterium isolated from a halophyte, Carex scabrifolia Steud.	Song GC, Yasir M, Bibi F, Chung EJ, Jeon CO, Chung YR	Int J Syst Evol Microbiol	10.1099/ijs.0.019919-0	2010	*
Phylogeny	Nocardioides hankookensis sp. nov., isolated from soil.	Yoon JH, Kang SJ, Lee MH, Oh TK	Int J Syst Evol Microbiol	10.1099/ijs.0.65267-0	2008	*

References

#15730

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 21479

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#32290

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #28530 (see below)

#60212 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 54522
#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#80382

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID323980.1 )

#28530

IJSEM 434 2008 ( DOI 10.1099/ijs.0.65267-0 , PubMed 18218945 )

* These data were automatically processed and therefore are not curated

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