Name: Nocardioides insulae DS-51
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 11120

Type strain:

Species: Nocardioides insulae

Strain Designation: DS-51

Culture col. no.: DSM 17944, CIP 109626, KCTC 19180, JCM 15308

Strain history: CIP <- 2007, DSMZ <- J.H. Yoon, KRIBB, Taejon, Korea: strain DS-51

NCBI tax ID(s): 1122610 (strain), 394734 (species)

SI-ID 297274

DSM 17944, KCTC 19180, CIP 109626, JCM 15308

Section

Nocardioides insulae DS-51 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from soil.

Gram-positive
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Propionibacteriales*
	Family	*Nocardioidaceae*
	Genus	*Nocardioides*
	Species	**Nocardioides insulae**
	Full Scientific Name (LPSN)	Nocardioides insulae Yoon et al. 2007

Information on morphological and physiological properties Morphology

[Ref.: #31895]	Gram stain	positive
[Ref.: #67771]	Gram stain	positive
[Ref.: #120139]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #31895]	Cell length	3.65 µm

[Ref.: #31895]	Cell width	0.8 µm

[Ref.: #31895]	Cell shape	rod-shaped
[Ref.: #120139]	Cell shape	rod-shaped

[Ref.: #31895]	Motility	no
[Ref.: #120139]	Motility	no
[Ref.: #69480]	Motility	no Confidence in %94.952

[Ref.: #18409]	Colony color	Lemon yellow (1012)
[Ref.: #18409]	Incubation period	10-14 days
[Ref.: #18409]	Cultivation medium used	ISP 2

[Ref.: #18409]	Colony color	Beige (1001)
[Ref.: #18409]	Incubation period	10-14 days
[Ref.: #18409]	Cultivation medium used	ISP 3

[Ref.: #18409]	Colony color	Beige (1001)
[Ref.: #18409]	Incubation period	10-14 days
[Ref.: #18409]	Cultivation medium used	ISP 4

[Ref.: #18409]	Colony color	Beige (1001)
[Ref.: #18409]	Incubation period	10-14 days
[Ref.: #18409]	Cultivation medium used	ISP 5

[Ref.: #18409]	Colony color	Lemon yellow (1012)
[Ref.: #18409]	Incubation period	10-14 days
[Ref.: #18409]	Cultivation medium used	ISP 6

[Ref.: #18409]	Colony color	Lemon yellow (1012)
[Ref.: #18409]	Incubation period	10-14 days
[Ref.: #18409]	Cultivation medium used	ISP 7

[Ref.: #31895]

Pigment production

yes

[Ref.: #18409]	Multicellular complex forming ability	no
[Ref.: #18409]	Medium Name (multicellularity)	ISP 2

[Ref.: #18409]	Multicellular complex forming ability	no
[Ref.: #18409]	Medium Name (multicellularity)	ISP 3

[Ref.: #18409]	Multicellular complex forming ability	no
[Ref.: #18409]	Medium Name (multicellularity)	ISP 4

[Ref.: #18409]	Multicellular complex forming ability	no
[Ref.: #18409]	Medium Name (multicellularity)	ISP 5

[Ref.: #18409]	Multicellular complex forming ability	no
[Ref.: #18409]	Medium Name (multicellularity)	ISP 6

[Ref.: #18409]	Multicellular complex forming ability	no
[Ref.: #18409]	Medium Name (multicellularity)	ISP 7

Information on culture and growth conditions Culture and growth conditions

[Ref.: #7280]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

[Ref.: #7280]

Culture medium growth

[Ref.: #7280]

Culture medium link

Medium recipe at MediaDive

[Ref.: #7280]

Culture medium composition

expand / minimize

[Ref.: #18409]

Culture medium

ISP 2

[Ref.: #18409]

Culture medium growth

[Ref.: #18409]

Culture medium composition

expand / minimize

[Ref.: #18409]

Culture medium

ISP 3

[Ref.: #18409]

Culture medium growth

[Ref.: #18409]

Culture medium composition

expand / minimize

[Ref.: #18409]

Culture medium

ISP 4

[Ref.: #18409]

Culture medium growth

[Ref.: #18409]

Culture medium composition

expand / minimize

[Ref.: #18409]

Culture medium

ISP 5

[Ref.: #18409]

Culture medium growth

[Ref.: #18409]

Culture medium composition

expand / minimize

[Ref.: #18409]

Culture medium

ISP 6

[Ref.: #18409]

Culture medium growth

[Ref.: #18409]

Culture medium composition

expand / minimize

[Ref.: #18409]

Culture medium

ISP 7

[Ref.: #18409]

Culture medium growth

[Ref.: #18409]

Culture medium composition

expand / minimize

[Ref.: #33314]

Culture medium

MEDIUM 328- for nutrient agar

[Ref.: #33314]

Culture medium growth

[Ref.: #33314]

Culture medium composition

expand / minimize

[Ref.: #120139]

Culture medium

CIP Medium 328

[Ref.: #120139]

Culture medium growth

[Ref.: #120139]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #7280]	growth	28 °C
[Ref.: #18409]	optimum	28 °C
[Ref.: #31895]	growth	10-34 °C
[Ref.: #31895]	optimum	30 °C
[Ref.: #33314]	growth	30 °C
[Ref.: #67770]	growth	28 °C
[Ref.: #67771]	growth	30 °C

[Ref.: #7280]	Temperature range	mesophilic
[Ref.: #18409]	Temperature range	mesophilic
[Ref.: #31895]	Temperature range	mesophilic
[Ref.: #33314]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #67771]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #31895]			optimum	8
[Ref.: #31895]			growth	6.5-8

Information on physiology and metabolism Physiology and metabolism

[Ref.: #31895]	Oxygen tolerance	aerobe
[Ref.: #67771]	Oxygen tolerance	aerobe
[Ref.: #120139]	Oxygen tolerance	obligate aerobe

[Ref.: #31895]

Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #31895]		NaCl		optimum	0-3	%

[Ref.: #67770]

Observation

quinones: MK-8(H₄)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #31895]	acetate ChEBI	+	carbon source
[Ref.: #31895]	cellobiose ChEBI	+	carbon source
[Ref.: #68379]	D-glucose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-mannitol ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-ribose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-xylose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	esculin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #31895]	galactose ChEBI	+	carbon source
[Ref.: #68379]	gelatin ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #31895]	glucose ChEBI	+	carbon source
[Ref.: #31895]	glutamate ChEBI	+	carbon source
[Ref.: #68379]	glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	lactose ChEBI	-	fermentation	* from API Coryne
[Ref.: #31895]	malate ChEBI	+	carbon source
[Ref.: #31895]	maltose ChEBI	+	carbon source
[Ref.: #68379]	maltose ChEBI	-	fermentation	* from API Coryne
[Ref.: #31895]	mannose ChEBI	+	carbon source
[Ref.: #31895]	nitrate ChEBI	+	reduction
[Ref.: #120139]	nitrate ChEBI	+	reduction
[Ref.: #68379]	nitrate ChEBI	-	reduction	* from API Coryne
[Ref.: #120139]	nitrite ChEBI	-	reduction
[Ref.: #31895]	pyruvate ChEBI	+	carbon source
[Ref.: #31895]	salicin ChEBI	+	carbon source
[Ref.: #68379]	sucrose ChEBI	+	fermentation	* from API Coryne
[Ref.: #31895]	trehalose ChEBI	+	carbon source
[Ref.: #68379]	urea ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #31895]	xylose ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #120139]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #31895]	alkaline phosphatase	+	3.1.3.1
[Ref.: #68379]	alkaline phosphatase	+	3.1.3.1	* from API Coryne, API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68379]	alpha-glucosidase	+	3.2.1.20	* from API Coryne
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68379]	beta-galactosidase	-	3.2.1.23	* from API Coryne, API zym
[Ref.: #68379]	beta-glucosidase	-	3.2.1.21	* from API Coryne, API zym
[Ref.: #68379]	beta-glucuronidase	-	3.2.1.31	* from API Coryne, API zym
[Ref.: #120139]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #31895]	cytochrome oxidase	+	1.9.3.1
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68379]	gelatinase	+		* from API Coryne
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68379]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API Coryne, API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #120139]	oxidase	+
[Ref.: #68379]	pyrazinamidase	+	3.5.1.B15	* from API Coryne
[Ref.: #68379]	pyrrolidonyl arylamidase	+	3.4.19.3	* from API Coryne
[Ref.: #120139]	urease	-	3.5.1.5
[Ref.: #68379]	urease	+	3.5.1.5	* from API Coryne
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #18409]	230	-	+	+	+	-	-	+	-	-	+	+	not determinedn.d.	-	-	-	-	-	-	+	-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #18409]	119	not determinedn.d.	+	+	+	-	+	-	-	+	-	+	+	-	-	-	+	-	-	-	-
[Ref.: #120139]	12011	-	+	+	+	-	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #7280]	Sample type/isolated from	soil
[Ref.: #7280]	Geographic location (country and/or sea, region)	Dokdo
[Ref.: #7280]	Country	Republic of Korea
[Ref.: #7280]	Country ISO 3 Code	KOR
[Ref.: #7280]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Soil

[Ref.: #67771]	Sample type/isolated from	From soil
[Ref.: #67771]	Geographic location (country and/or sea, region)	Dokdo
[Ref.: #67771]	Country	Republic of Korea
[Ref.: #67771]	Country ISO 3 Code	KOR
[Ref.: #67771]	Continent	Asia

[Ref.: #120139]	Sample type/isolated from	Environment, Soil
[Ref.: #120139]	Geographic location (country and/or sea, region)	Dokdo island
[Ref.: #120139]	Country	Republic of Korea
[Ref.: #120139]	Country ISO 3 Code	KOR
[Ref.: #120139]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental

#Terrestrial

#Soil

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #18409]	Biosafety level	1	German classification
[Ref.: #7280]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #120139]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #7280]	Nocardioides insulae strain DS-51 16S ribosomal RNA gene, partial sequence	DQ786794	1484	ENA	394734 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Nocardioides insulae DSM 17944	GCA_000422825	scaffold	GenBank	1122610 tax ID
[Ref.: #66792]	Nocardioides insulae DSM 17944	1122610.3	wgs	PATRIC	1122610 tax ID	*
[Ref.: #66792]	Nocardioides insulae DSM 17944	2524614651	draft	JGI IMG/M	1122610 tax ID	*

[Ref.: #7280]	GC-content	71.1 mol%
[Ref.: #67770]	GC-content	71.1 mol%	high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Nocardioides insulae DSM 17944 DSM 17944 NCBI: GCA_000422825.1
[Ref.: #69481]	spore-forming	no	80	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Nocardioides insulae DSM 17944 PATRIC: 1122610.3
[Ref.: #69480]	motile	no	97.236	yes
[Ref.: #69480]	flagellated	no	98.799	no
[Ref.: #69480]	gram-positive	yes	94.233	no
[Ref.: #69480]	anaerobic	no	99.099	no
[Ref.: #69480]	aerobic	yes	94.991	yes
[Ref.: #69480]	halophile	no	77.304	no
[Ref.: #69480]	spore-forming	no	81.438	yes
[Ref.: #69480]	glucose-util	yes	90.445	yes
[Ref.: #69480]	thermophile	no	98.465	yes
[Ref.: #69480]	glucose-ferment	no	94.061	yes

Availability in culture collections External links

[Ref.: #7280]

Culture collection no.

DSM 17944, CIP 109626, KCTC 19180, JCM 15308

[Ref.: #80369]

SI-ID 297274

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Nocardioides fonticola sp. nov., a novel actinomycete isolated from spring water.	Chou JH, Cho NT, Arun AB, Young CC, Chen WM	Int J Syst Evol Microbiol	10.1099/ijs.0.65810-0	2008	*
Phylogeny	Nocardioides insulae sp. nov., isolated from soil.	Yoon JH, Kang SJ, Lee CH, Oh TK	Int J Syst Evol Microbiol	10.1099/ijs.0.64612-0	2007	*

References

#7280

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 17944

#18409

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#31895

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #28154 (see below)

#33314 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#68379

Automatically annotated from API Coryne .

#68382

Automatically annotated from API zym .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#80369

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID297274.1 )

#120139 Collection of Institut Pasteur ; Curators of the CIP; CIP 109626

#28154

IJSEM 136 2007 ( DOI 10.1099/ijs.0.64612-0 , PubMed 17220455 )

* These data were automatically processed and therefore are not curated

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Nocardioides insulae DSM 17944 DSM 17944

Nocardioides insulae DSM 17944

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